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Citations for New England Biolabs :
451 - 500 of 3154 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... DNA was ligated by incubation at 16°C for 5h in 4 ml volume using 100U of T4 DNA ligase (NEB). After reverse crosslinking and Proteinase K treatment (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: Protein samples were generated by collecting the pellet for 1 ml of cells at OD600 ∼ 1.0 and lysing with 100 µl 1X SDS sample buffer (New England Biolabs) and DTT by boiling for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... 1× CutSmart buffer (50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate,100 μg/mL BSA, pH 7.9, NEB), 20 U RiboLock ...
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bioRxiv - Microbiology 2021Quote: ... the inserts and the plasmids were mixed with Gibson master mix 2x (100 µL 5X ISO Buffer, 0.2 µL 10,000 U/mL T5 exonuclease (NEB #M0363S), 6.25 µL 2,000 U/mL Phusion HF polymerase (NEB #M0530S) ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 µL 100 µM dNTPs, 0.3 µL 50 mg/mL BSA, 6 µL blunting enzyme mix from quick blunting kit, NEB). Prior to ligation ...
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bioRxiv - Molecular Biology 2023Quote: ... and 0.5 μl of 100 mM phenylmethylsulfonyl fluoride] and incubated with micrococcal nuclease (2 × 103 U/ml; New England Biolabs) for 2 hours at 4°C ...
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bioRxiv - Genomics 2024Quote: ... 500 µl lysed cells were then transferred to a tube containing 4.5 ml digestion buffer (1% Triton X-100, 1x CutSmart buffer (New England Biolabs)) ...
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bioRxiv - Microbiology 2021Quote: ... 250 ng of plasmids pBR322 or pUC19 (NEB) were combined with 15 µl of protein fractions ...
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bioRxiv - Immunology 2020Quote: ... 50 ng/µL lambda DNA (New England Biolabs), 5 pmol/µL methylation or nonmethylation-specific probe ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 ng/μl ET SSB (New England Biolabs), 3 mM MnCl2 (Fisher Scientific) ...
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bioRxiv - Genetics 2023Quote: ... 0.5 ng of unmethylated phage-λ DNA (NEB) was spiked into each DNA sample prior to shearing to allow assessment of the efficiency of the bisulfite-conversion reaction ...
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bioRxiv - Developmental Biology 2021Quote: ... Dissected tissue was then washed 2X in chilled nuclei extraction buffer (NEB: 10 mM HEPES ...
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bioRxiv - Genomics 2019Quote: ... Nuclei were extracted by incubation of cells in Nuclear Extraction Buffer (NEB) containing 10 mM Tris-HCl ...
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bioRxiv - Microbiology 2019Quote: ... The Monarch® DNA Gel Extraction Kit (New England BioLabs, Ipswitch, MA) was used to isolate PCR products during extension overlay PCR ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: ... RNA extraction was performed using the Monarch Total RNA Miniprep Kit (NEB), according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The Monarch® DNA Gel Extraction Kit (New England BioLabs, Ipswitch, MA) was used to isolate PCR products during PCR ...
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bioRxiv - Immunology 2022Quote: ... purified by agarose gel isolation (Monarch DNA Gel Extraction Kit, NEB (T1020L)) and ligated into LMP-destGFP (Lindner et al ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified using the Monarch DNA Gel Extraction Kit (NEB) and eluted in 20 µl ddH2O ...
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bioRxiv - Molecular Biology 2021Quote: RNA extraction was performed with Monarch Total RNA Miniprep Kit (NEB #T2010) following the manufacturer’s indications ...
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bioRxiv - Microbiology 2022Quote: ... RNA extraction was performed with the Monarch Total RNA Miniprep Kit (NEB). DNAse treatment was performed as described above and cDNA was synthesized using LunaScript RT Supermix Kit (NEB) ...
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bioRxiv - Genomics 2022Quote: RNA extraction was performed using the Monarch Total RNA Miniprep Kit (NEB) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2022Quote: 20 μl of in extraction buffer equilibrated streptavidin magnetic beads (S1420, NEB) were added to 1 ml of the free biotin depleted protein extracts ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA extractions were DNase treated (RQ1 RNase-free DNase, New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... using the Monarch® DNA Gel Extraction Kit (New England Biolabs, USA). In vitro transcription of this template was performed using the mMESSAGE mMACHINE T7 kit (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... RNA extraction was performed using Monarch® Total RNA Miniprep kit (NEB) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... they were purified using Monarch DNA Gel Extraction kit (New England Biolabs) and assembled by two-step PCR (supplementary methods ...
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bioRxiv - Bioengineering 2024Quote: ... gDNA extraction using Monarch® Genomic DNA Purification Kit (New England Biolabs), and histology sectioning.
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bioRxiv - Bioengineering 2024Quote: ... gDNA extraction using Monarch® Genomic DNA Purification Kit (New England Biolabs), and histology sectioning.
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bioRxiv - Molecular Biology 2023Quote: ... Inserts were acquired by PCR using KOD Hotstart Mastermix from NovaGen and purified from gel using Monarch DNA Gel Extraction kit from NEB. All cloning was performed in E ...
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bioRxiv - Microbiology 2019Quote: ... Colonies were selected using a gentamycin marker and blue/white colony screening in a background of dH5-alpha cells (New England Biolabs, UK). The resulting construct was transformed into PA14 and the clone used for assays was verified by Sanger sequencing (University of Sheffield ...
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bioRxiv - Molecular Biology 2020Quote: ... The amplicons were analyzed by 1.5% agarose gel electrophoresis with ethidium bromide staining and using a DNA ladder marker (2 log DNA ladder from New England Biolabs, USA). Amplicon bands were observed under UV light ...
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bioRxiv - Cell Biology 2021Quote: ... on a Mini Gel Tank (Thermo #A25977) using MES SDS Running Buffer (Thermo #B0002) alongside broad range markers (11-245KDa, NEB #P7712S). Each lane contained 20 μg of protein ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the plasmids for the Blasticidin and Zeocin resistance marker-based systems were introduced to T7 Express chemically competent cells (NEB #C2566I) according to the manufacturer’s high-efficiency transformation protocols ...
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bioRxiv - Evolutionary Biology 2022Quote: ... a total of 575 embryos were injected with a mixture containing 300 ng/μL of sgRNA (one guide) and 600 ng/μL of Cas9 protein (NEB, M0641) while for Dll ...
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bioRxiv - Developmental Biology 2021Quote: ... After annealing the complex an equimolar amount was mixed with 1000 ng Cas9 recombinant protein (NEB; final concentration 20 ng/μL) and incubated at RT for 15 min ...
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bioRxiv - Genomics 2021Quote: ... 510 ng (6 µl at 85 ng/µl) of DNA was digested using endonucleases EcoRI and MseI (New England BioLabs, Inc.) after which barcoded (EcoRI cut site ...
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bioRxiv - Genomics 2023Quote: ... The gel-purified DGP-4 fragments (200 ng) and p200 vector (200 ng) were mixed and ligated in a 50 µL T4 ligation reaction (NEB, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Recombination was performed by incubating the linearized vector (330 ng) with the amplicons pool (50 ng) and the NEBuilder HiFi DNA Assembly (NEB #E2621) 50°C for 15 minutes to one hour ...
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bioRxiv - Genetics 2024Quote: ... 400 nM of each of the flanking primers PS1057-NGS-F and PS1057-NGS-R (S2 File) and 0.1 units/µl of LongAmp Taq DNA polymerase (NEB Cat. # M0323L). In a second amplification step ...
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bioRxiv - Cell Biology 2024Quote: ... following the manufacturer protocols (100-6472 B1, PN 100-5875 C1) and reagents (Fluidigm,100-6297; Fluidigm, 100-5580; New England Biolabs, M0293S; TEKnova, 10221). Real-time PCR data was collected using the Biomark HD system with a 96.96 IFC chip using Fluidigm Delta-Gene Assays on the pre-amplified cDNA ...
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bioRxiv - Microbiology 2021Quote: ... Firefly luciferase (FLuc) RNA served as the spike in control and was generated using HiScribe T7 Quick RNA Synthesis kit (NEB, Ipswich, MA, USA) using linearized plasmid DNA encoding FLuc gene as template ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 mL of culture was removed and treated with DNase I (New England Biolabs; 4 µL and 25µL of DNaseI buffer) for 1 h at 30 °C to remove any extracellular DNA ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were centrifuged for 5 minutes at 5000rpm at 4°C and supernatant treated with 5 mg/ml of proteinase K (New England Biolabs #P8102) for 1 hour at 50°C ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μl of the diluted samples was then denatured by addition of 4 M urea and Proteinase K (40 U/ml; New England Biolabs #P8107S), incubated for 5 min at 65°C ...
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bioRxiv - Microbiology 2023Quote: ... Digestion products were diluted by the addition of 4 mL 1.1× T4 DNA Ligase Reaction Buffer (New England BioLabs® Inc) with 1% Triton X-100 and incubated for 1 h at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... Fluorescent probes were diluted in Hybridization Buffer (10% dextran sulfate, 0.1mg/ml salmon sperm ssDNA, 100 µl vanadyl ribonucleoside (NEB biolabs), 20ug/ml RNAse-free BSA ...
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bioRxiv - Cell Biology 2021Quote: One plug per sample was melted at 68°C in 1 mL 100 mM MES buffer pH 6.5 prior to addition of β-agarase (New England Biolabs) and incubation overnight at 42°C ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μl of MBP-SYCP1N at 2 mg/ml was incubated for 20 minutes on ice with 100 ul amylose resin slurry (NEB) equilibrated in 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... Bacterial lysis was completed by incubating for 3 h at 55°C in the presence of 1% SDS and 100 μg/ml proteinase K (NEB). Lysates from both methods were extracted twice with equal volumes of phenol– chloroform–isoamyl alcohol (25:24:1) ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorescent probes diluted in Hybridization Buffer (10% dextran sulfate, 0.1mg/ml salmon sperm ssDNA, 100 μl vanadyl ribonucleoside (NEB biolabs), 20ug/ml RNAse-free BSA ...