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Citations for New England Biolabs :
1 - 50 of 1023 citations for Dig 6C SAH 1a b c since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Five POWV-LI9 fragments were amplified from viral cDNA (Figure 1A,B) using high-fidelity Phusion polymerase (NEB) and corresponding paired primers (Table S2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Variants were subsequently cloned into pGW1-6C by Gibson Assembly (NEB). Nonsense mutations were generated by site-directed mutagenesis using primers onk046 and onk047 to change the rpoB start codon TTG to stop codon TAG ...
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bioRxiv - Microbiology 2020Quote: pCrPV-3 and pCrPV-1A-DcDV-1A were linearized by digestion with BamHI-HF (New England Biolabs, Ipswich, Massachusetts). Wild-type and CrPV-DcDV RNA was prepared by in vitro transcription of 1 µg linearized plasmid using the mMESSAGE mMACHINE T7 transcription kit (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... B and C oligonucleotides were phosphorylated with T4 polynucleotide kinase (NEB, Cat #M0201L) according to the manufacturer recommendations ...
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bioRxiv - Molecular Biology 2023Quote: Expression vectors encoding His6/SUMO-tagged SAHS proteins were obtained from Twist Bioscience and transformed into LEMO21(DE3) competent cells (New England Biolabs) according to the provider’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Coli vectors expressing SAHS proteins without SUMO tags were ordered and synthesized from Twist Bioscience and transformed into LEMO21(DE3) competent cells (NEB). To test for the effects of intracellular SAHS expression ...
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bioRxiv - Developmental Biology 2020Quote: ... and dlk-1a were cloned into the two-hybrid vectors using HiFi Assembly (NEB).
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bioRxiv - Developmental Biology 2021Quote: ... RNA Probes were labeled with DIG (New England Biolabs) or DNP-11-UTP (Perkin Elmer ...
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bioRxiv - Microbiology 2022Quote: ... CrPV 5’UTR-1A-GFP-3’ UTR was generated using Gibson assembly (NEB Gibson assembly). The respective mutants were generated using Site directed mutagenesis ...
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bioRxiv - Microbiology 2021Quote: ... two constructs (Fig. 1A) were made using a modified pgRNA-bacteria plasmid (NEB, Addgene plasmid # 44251). This plasmid ...
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bioRxiv - Microbiology 2022Quote: ... with the following modifications (Fig. 1A): The input RNAs were treated with Antarctic Phosphatase (NEB, Ipswich, MA) for 1 h at 37°C to remove any 5’end phosphates ...
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bioRxiv - Biochemistry 2021Quote: ... 1000 base pairs upstream and downstream from the clpP2 gene were amplified by PCR using the A–B and C–D primer pairs from table 3 (Phusion polymerase, GC buffer, New England Biolabs). The PCR products were purified with E.Z.N.A ...
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bioRxiv - Biochemistry 2021Quote: ... 700 bp upstream and downstream were amplified using the A–B and C–CD primer pairs from Table 2 (Phusion polymerase, GC buffer, New England Biolabs). The 2×myc tag was added to the B primers as overhangs ...
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bioRxiv - Microbiology 2023Quote: ... These PCRs were performed with the specified primer sets (a, c and e or b, d and f) and Q5 High-Fidelity Polymerase (NEB) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Full length human NHSL1-A1 including Exon 1a and excluding exons 3 and 6 was generated by NEB HIFI assembly using a synthetic DNA fragment (gBlocksTM ...
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bioRxiv - Cancer Biology 2024Quote: ... whereas SPARC and GCDH were cloned in pCMV-3Tag-1A overexpression plasmid (amplified using Phusion Polymerase, NEB, M0530S). YEATS2 was cloned between SalI and SmaI sites ...
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bioRxiv - Microbiology 2022Quote: ... Briefly 5 µg pAc CrPV 5’UTR-1A-GFP-3’ or pCrPV-3 plasmids were linearized with Eco53KI (NEB) or 5 µg pTOPO dsRNA plasmids with EcoR1 (NEB ...
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bioRxiv - Biochemistry 2022Quote: The standard N-glycoprotein bovine pancreatic ribonuclease B (RNase B, New England Biolabs, USA) was used as a substrate to measure NGLY1 activity ...
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bioRxiv - Molecular Biology 2021Quote: ... DIG-labelled probes were synthesized by in vitro transcription (New England BioLabs Inc.) with appropriate polymerases ...
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bioRxiv - Biophysics 2021Quote: ... coli B ER2566 from NEB; ampicillin ...
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bioRxiv - Cell Biology 2022Quote: ... EIF2AK2 and EIF2S1 sequences were inserted into pET-His 1a vector using the Gibson Assembly Cloning Kit (New England Biolabs) as described by the manufacturer.
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bioRxiv - Biochemistry 2021Quote: ... 1000 base pairs upstream and downstream from the C-terminus of clpP1 were amplified by PCR using the A–B and C–D primer pairs from Table 2 (Phusion polymerase, GC buffer, New England Biolabs, Ipswich, United States). For clpP2 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Single guide RNA (sgRNA) targeting pOXA-48 pemK gene (Fig.1A) was introduced into pLC10-Kan by golden gate assembly34 (New England Biolabs, UK). pLC10-Apra was constructed by exchanging the aph(3’)-Ia gene (Kanamycin resistance ...
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bioRxiv - Molecular Biology 2024Quote: TeloTags were prepared in 100μL reactions with 5mM of each of the 6 permutations of telomere splint Extended Data Fig.1A) and 30 mM of biotinylated adapter in HiFi Taq DNA Ligase Reaction Buffer (NEB, Cat. M0647S). Annealing was done by heating to 99 degrees and slowly decreasing the temperature 1°C /min in a Veriti™ 96-Well Thermal Cycler (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... the 181-bp and 376-bp amplicon products (Figure 1-figure supplement 1A) were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA) and sent for Sanger DNA sequencing at the OHSU Vollum Sequence Core (Portland ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin (DIG)-labeled RNA probes were synthesized using T7 or T3 RNA polymerases (NEB, USA) with the DIG RNA Labeling Mix (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... corresponding to a portion of PfRNF1 (RP2; Fig. 1A) was expressed as a maltose-binding fusion protein using the pMAL™c5X-vector (New England Biolabs, Ipswich, USA). To this end ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Specific DIG-labelled RNA probes were produced using HiScribe Sp6 and T7 in vitro transcription kits (NEB). The sequences of the probes are in the Supplementary Table 5 ...
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bioRxiv - Biochemistry 2021Quote: ... bovine RNase B and fetuin (New England Biolabs) by PNGase F (New England Biolabs ...
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bioRxiv - Developmental Biology 2024Quote: ... gel purified and transcribed with DIG RNA labeling mix with either T7 RNA polymerase (NEB, M0251S; antisense probe), or SP6 RNA polymerase (NEB ...
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bioRxiv - Microbiology 2020Quote: ... B are ligated first by T4 DNA ligase (NEB) in 40μl system ...
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bioRxiv - Immunology 2023Quote: ... 1μl Cas9 (diluted 1:3 in diluent B, NEB), 1μl water (water was replaced with 100ng/μl trib1 RNA for cop1 experiments) ...
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bioRxiv - Immunology 2024Quote: ... using BsmBI (New England Biolabs, supporting table I-b). The plasmid containing the gRNA targeting mSppl3 or mUgcg (lentiCRISPR-v2-mSppl3gRNA-GFP or lentiCRISPR-v2-mUgcggRNA-GFP ...
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bioRxiv - Genomics 2024Quote: ... (b) PURExpress T7 recombinant IVT system (NEB Cat.#E6800L), for inserts in pTH6838 ...
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bioRxiv - Developmental Biology 2023Quote: ... The next day samples were washed twice with dig-wash (wash buffer with 0.025% digitonin) and then resuspended in 150 µl dig-wash containing pA-MNase (New England Biolabs 700 ng/ml ...
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bioRxiv - Biochemistry 2021Quote: ... 125 to 75 U/ 5 μg RNase B (Cat.P7817S, NEB) and fetuin (Cat ...
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bioRxiv - Molecular Biology 2020Quote: ... Cas9 protein (IDT) was suspended with a Diluent B (NEB) to make 1 μM solution ...
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bioRxiv - Developmental Biology 2020Quote: ... Plasmids were linearized with appropriate restriction enzymes and cRNA preparation was carried out using Roche DIG-labeling reagents and RNA polymerases (NEB). We used previously published probes for olig2 (H.-C ...
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bioRxiv - Cell Biology 2022Quote: Template DNA for producing DIG-labelled RNA probes were obtained by PCR by using Q5 High-Fidelity DNA polymerase (M0491S, New England Biolabs) with the primers listed in Table S2 ...
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bioRxiv - Genomics 2022Quote: The 2.2 kb DNA was ligated with 1 kb biotin- and dig-labeled DNA through SbfI- and XbaI-overhangs by T4 DNA ligase (NEB Biolabs). The ligated products were attached to the anti-dig treated surface of reaction chamber and streptavidin-coated magnetic beads ...
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bioRxiv - Developmental Biology 2021Quote: ... A T7 RNA polymerase binding site with short 5’-tail (aaaaTAATACGACTCACTATAG) was added to reverse primers for transcription using T7 RNA polymerase incorporating DIG labelled ribonucleotides (NEB, Roche). PCR amplified probe templates were confirmed by sanger sequencing.
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bioRxiv - Biophysics 2021Quote: ... The ligation of the DIG-handles and biotinylated DNA fragments was carried out overnight using T4 DNA ligase (New England Biolabs, UK).
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bioRxiv - Cell Biology 2023Quote: ... The odf3l2a template was amplified from total RNA with T3 and T7 sites on the forward and reverse primers respectively and the DIG-labeled antisense probe was synthesized directly from purified PCR product using T7 RNA polymerase (NEB M0251S). The ankef1a and saxo2 probe templates were amplified from total RNA with gene specific primers and cloned into the pCR4-TOPO vector (Thermo Fisher 450071) ...
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bioRxiv - Genomics 2022Quote: The 2.2 kb DNA was ligated with 1 kb biotin- and dig-labeled DNA through SbfI- and XbaI-overhangs by T4 DNA ligase (NEB Biolabs). The ligated products were attached to the anti-dig treated surface of reaction chamber and streptavidin-coated magnetic beads ...
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bioRxiv - Biochemistry 2022Quote: ... RNase B from bovine pancreas (New England Biolabs, Ipswich MA, USA), and horse radish peroxidase (HRP ...
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bioRxiv - Microbiology 2024Quote: ... coli B strain was dephosphorylated with 0.4 U/µL QuickCIP (NEB) in 1 x rCutSmart buffer (NEB ...
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bioRxiv - Microbiology 2024Quote: ... (b) Heat labile Shrimp Alkaline Phosphatase (rSAP) (1 µl) (NEB #M0371S) was used to remove terminal phosphates that could interfere with terminal extension by incubating in rCutSmart buffer (3.5 µl ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli cells were incubated at 37°C (30°C for NEB stable), 200 min-1 either in 5 ml (for a high copy ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli cells were incubated at 37°C (30°C for NEB stable), 200 min-1 either in 5 ml (for a high copy ...
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bioRxiv - Cancer Biology 2024Quote: ... was PCR amplified (14 cycles: 10sec 98°C, 30 sec 57°C, 20 sec 72°C) with Phusion polymerase (New England Biolabs (NEB)) ...