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Citations for New England Biolabs :
201 - 250 of 1817 citations for Dengue Virus serotype 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... in NEBuffer 2 (NEB) for 2 h at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM ATP (NEB) in a total volume of 25 μl ...
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bioRxiv - Genomics 2023Quote: ... 1X NEBuffer 2 (NEB), 0.5 μM P5 primer ...
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bioRxiv - Genetics 2020Quote: ... followed by gap repair by adding 2 µl of 10× NEBuffer 2 (NEB), 3 µl of dNTPs (2.5 mM each ...
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bioRxiv - Molecular Biology 2021Quote: ... and 2’O-methylated using Vaccinia VP39 (2’O Methyltransferase) (New England Biolabs), then purified by phenol-chloroform extraction and ethanol precipitation.
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bioRxiv - Molecular Biology 2020Quote: ... Lysate was incubated with 150 μl of oligo (dT)25 cellulose beads (New England BioLabs, cat no. S1408S) for 15 min at room temperature ...
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bioRxiv - Physiology 2022Quote: ... Diluted lysate was then incubated with 9 ml of packed amylose resin (catalog no. E8021S, New England Biolabs) and incubated at 4°C for 2h with gentle rotation ...
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bioRxiv - Microbiology 2022Quote: Cell lysates were prepared as described above used as DNA template for PCR amplification using Phusion polymerase (NEB) and oligonucleotides that anneal 150 bp outside of wzc ...
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bioRxiv - Cell Biology 2019Quote: ... 5-10 µg of total protein (9 µl of lysate) were treated with Endo Hf (New England Biolabs) for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... A 10% fraction of the chromatin lysate was digested using 200 U of Taq I (New England Biolabs) at 60°C for 7 h ...
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bioRxiv - Biochemistry 2021Quote: ... The second fraction of the clarified lysate was loaded onto 200 μl of Amylose Resin (New England Biolabs) equilibrated in Amy-Strep wash buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... E protein glycosylation status was determined by treating RVP lysates with PNGase F (Cat#P0704S, New England Biolabs) for 3 hours at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... DNA lysate was used as genotyping PCR template with Q5 Hot Start High-Fidelity 2X Master Mix (NEB) and enhancer specific genotyping primer pair spanning the upstream and downstream Cas9-guide RNA cleavage sites (Table S19) ...
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bioRxiv - Developmental Biology 2019Quote: Pacy-2::acy-2 genomic was generated with a Gibson assembly cloning kit (NEB) by assembly of the following two DNA fragments ...
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bioRxiv - Immunology 2021Quote: ... Samples were added to second-strand synthesis mix containing 2× NEB buffer 2 (NEB), 625 nM dNTP Mixture (NEB) ...
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bioRxiv - Developmental Biology 2022Quote: ... 50 ng PCR product was incubated with 2 µL 10X NEBuffer 2 (NEB, B7002S) and nuclease-free water adding up to 19 µL using the following program ...
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bioRxiv - Biochemistry 2019Quote: 2 μg of nucleosomes were incubated with 2 Kunitz units of Micrococcal nuclease (NEB) in buffer containing 10 mM Tris HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μl 1M MgCl2 and 2 μl of 1 U/μl Xrn1 (M0338, NEB) were added after RNase H digest ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 units DNase I (NEB), or both for 30 min at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... α2-3,6,8,9 neuraminidase A (NEB), LB Broth (BD Difco™) ...
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bioRxiv - Microbiology 2020Quote: ... 2) NEB Q5 (NEB M0491), 3 ...
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bioRxiv - Molecular Biology 2022Quote: ... T4 RNA ligase 2 (NEB) and RiboLock RNase inhibitor (ThermoFisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... 2 µL DpnI (NEB # R0176L) is then added and the reaction incubated 1h at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 units DNase I (NEB) or both for 1 hour at 37°C and resolved on a denaturing urea polyacrylamide gel (15% ...
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bioRxiv - Immunology 2020Quote: ... 2 U DNase I (NEB) was added to IVT mixture and further incubated at 37 °C for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 units DNase I (NEB), or both for 1 h at 37°C and resolved on a denaturing urea polyacrylamide gel (20% ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 μL DpnI (NEB # R0176L) is then added and the reaction incubated 30 min at 37°C ...
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bioRxiv - Biochemistry 2019Quote: ... 2 U UDG (NEB, M0280S), and 1X UDG buffer (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... in 1× NEBuffer 2 (NEB) at 37°C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... in 1x NEBuffer 2 (NEB) at 37°C for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... 2 μl Proteinase K (NEB) was added and samples were incubated at 56°C for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... 2 µL factor mix (NEB), 250 nM pre-incubated 50S subunit ...
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bioRxiv - Plant Biology 2023Quote: ... 2 μL ATP (NEB, B0202S), 2 μL 10X restriction digest buffer (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... and set 2 (NEB #7500S) according to the manufacturer’s protocol with minor modifications as noted below ...
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bioRxiv - Developmental Biology 2023Quote: ... and Set 2 (E7500S, NEB). Quality of the final library preparation was analysed on the Bioanalyzer using the High Sensitivity DNA reagents kit and cassettes (5067-4626 ...
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bioRxiv - Biochemistry 2023Quote: ... and 2 mM ATP) (NEB) at 30 °C for 1 h ...
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bioRxiv - Immunology 2022Quote: ... with 2 mM MgCl2 (NEB), 0.2mM dNTPs (made in house) ...
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bioRxiv - Genomics 2024Quote: ... 2 μl of BSA (NEB), 15 μl of dNTPs 10 mM ...
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bioRxiv - Cell Biology 2020Quote: ... 40 µl of the cleared lysates were incubated with 1 µl Lambda Protein Phosphatase (λ phosphatase) (New England Biolabs), 5 µl 10x buffer for Protein MetalloPhosphatases (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mg of cell lysate was cleared with protein A magnetic beads to remove non-specific interactions (S1425S, NEB) and incubated overnight with anti-ZAP antibody (Proteintech 16820-1-AP ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were centrifuged at 30,597xg and 4°C for 20min and the supernatant was applied to amylose resin (NEB). The column was washed with 10 column volumes (CV ...
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bioRxiv - Cell Biology 2021Quote: Whole cell lysates or eluted proteins after affinity purification were treated with Protein Deglycosylation Mix II (NEB, Cat# P6044), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was incubated with 150 μl of oligo (dT)25 cellulose beads (New England BioLabs, cat no. S1408S) for 15 min at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and protein was purified on amylose resin (NEB) including a high salt wash with buffer containing 25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... Then homogenized lysate incubated with 0.80 mL of pre- equilibrated oligo(dT)25 magnetic beads (New England Biolabs, #S1419S) for 1 h at 4°C with gentle rotation ...
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bioRxiv - Cell Biology 2021Quote: ... COS-7 cell lysates from FLAG-ACBD4(wACBD5_FFAT)/5 (mFFAT) expressing cells were treated for 1 h with λPP (New England BioLabs) as described above ...
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bioRxiv - Cancer Biology 2019Quote: ... 25ug of each protein lysate were diluted into 3xSDS gel loading buffer (New England Biolabs Cat# B7703S, Lpswich, MA) and heated to 95°C for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Lysates containing 6xHis-MBP-OmpW and 6xHis-MBP-EF-Tu were incubated on amylose resin (New England Biolabs, E8021L) (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... and the remaining cytoplasmic lysate was supplemented with 0.4M CaCl2 and 4000 gel units micrococcal nuclease (New England Biolabs), and incubated at 37°C (30 min ...
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bioRxiv - Cell Biology 2023Quote: ... total proteins isolated from cell lysates were digested with Endoglycosidase H (endo H) enzyme (New England Biolabs, catalog #: P0703L) with G5 reaction buffer at 37°C according to manufacturer’s instruction and the published procedure 15 ...