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Citations for New England Biolabs :
1 - 50 of 1819 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Protein lysates were generated using cell lysis buffer (NEB) and brief sonication on ice ...
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bioRxiv - Microbiology 2021Quote: ... cell lysates were mixed with glycoprotein denaturing buffer (New England Biolabs) and incubated at 100 °C for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell lysates were incubated with H4K5 Bu antibody (PTM Biolabs) or preimmune IgG per sample and 25 μl of magnetic protein G Dynabeads (catalog no ...
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bioRxiv - Biochemistry 2021Quote: Whole cell lysate or 5 μL of PURExpress (New England BioLabs) reaction was loaded onto a nitrocellulose membrane (Pall Corporation ...
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bioRxiv - Molecular Biology 2024Quote: ... cell lysates were first incubated with Deglycosylation Mix Buffer 2 (NEB) at 75°C for 10 min ...
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bioRxiv - Physiology 2021Quote: ... Enzymatic antigen retrieval with proteinase K (Biolabs, 2μg/mL) was used prior to incubation with antibodies ...
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bioRxiv - Microbiology 2021Quote: ... cell lysates were treated with endoglycosidase H (Endo H) (New England BioLabs) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were supplemented with 1X Protein MetalloPhosphatase buffer (New England Biolabs) and 1 mM MnCl2 ...
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bioRxiv - Biochemistry 2019Quote: ... The cleared cell lysate was applied to Amylose resin (New England Biolabs) followed by washing with buffer A and elution with buffer A containing 10 mM maltose ...
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bioRxiv - Cell Biology 2023Quote: ... After 24 h cell lysates were treated with PNGase-F (NEB, P0704L) or water by following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared cell lysate was applied to Amylose resin (New England Biolabs), followed by washing with buffer A and elution with buffer A containing 10 mM maltose ...
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bioRxiv - Cell Biology 2020Quote: ... 50 µg cell lysate was treated with 80 units of lambda-phosphatase (NEB) at 30°C for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... Cell lysates were applied to a column containing amylose resin (New England Biolabs). The beads were washed with at least ten column volumes of buffer containing 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: HeLa cell lysates were incubated with PNGase F (New England Biolabs, NEB-P0704S) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: HeLa cell lysates were incubated with PNGase F (New England Biolabs, NEB-P0704S) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... cell lysates were incubated in 40 μg/ml PNGase F (New England Biolabs, MA) for 2 hr at 37 °C to cleave all N-glycans before immunoblotting 62 ...
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bioRxiv - Plant Biology 2019Quote: ... and 300mL of the cell lysate was mixed with amylose resin (New England Biolabs) for the MBP-tagged proteins or glutathione sepharose resin (GE Healthcare Life Sciences ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were additionally treated with 16 units of PNGase F (New England Biolabs) per μL of lysate complemented with 1% NP-40 ...
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bioRxiv - Molecular Biology 2023Quote: ... rAAV titering was performed on crude cell lysates using dye-based qPCR (NEB, M3003L) with primers against ITR regions ...
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bioRxiv - Microbiology 2023Quote: ... lysates (1 mg) were incubated with MNase (10 units/1 mg lysate; NEB #M0247S) at 37°C for 60 min ...
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bioRxiv - Cell Biology 2020Quote: ... The cell lysate was treated with 200 mg/ml of DNAse I (New England BioLabs) for 10 min at 37°C followed by 10 min at room temperature and then clarified by centrifugation at 1500 g for 15 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Glycoprotein denaturation of the cell lysate was performed with 1x glycodenaturing buffer (New England Biolabs) at 95°C for 10 mins ...
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bioRxiv - Cell Biology 2019Quote: ... 50μg of mitotic cell lysates were treated with 400U of λ-phosphatase (New England Biolabs) at 30°C for 1 hour in a total volume of 50μl PMP phosphatase buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Denatured cell lysate was then deglycosylated using 500 U of PNGase F (New England Biolabs) in 1x glycobuffer 2 (New England Biolabs) ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg cell lysate was mixed with 3,200 U λ protein phosphatase (New England Biolabs) and incubated at 37 °C for 1 h to achieve complete protein dephosphorylation ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were denatured by boiling with 3X SDS Blue Loading Buffer (New England Biolabs). Samples were resolved on SDS-PAGE gel and transferred onto nitrocellulose membranes (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin in the cell lysate was digested using Microccocal nuclease (MNase, New England BioLabs, M0247S) at room temperature for 5 min and 5 ul 0.25 mM EDTA was added to stop the reaction ...
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bioRxiv - Pathology 2020Quote: ... Heat mediated antigen retrieval pretreatment using 10mM Sodium Citrate buffer (Vectors Biolabs) was used ...
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bioRxiv - Cell Biology 2021Quote: ... COS-7 cell lysates from FLAG-ACBD4/5 (mFFAT) expressing cells were treated for 1 h with λPP (New England BioLabs) as described above ...
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bioRxiv - Cell Biology 2022Quote: ... cleared lysate was incubated without or with PNGase F (NEB, 1 μl per 40 μl lysate) for 30 min at 37 °C ...
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bioRxiv - Developmental Biology 2019Quote: ... Crude cell lysate was passed through a Sepharose column containing 5mL amylose resin (New England Biolabs) and the MBP-tagged GFP-NT-Lysenin fusion protein was eluted with fresh 10 mM maltose in 1X PBS (pH 7.4) ...
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bioRxiv - Biochemistry 2022Quote: ... Deglycosylated cell lysates or conditioned media were prepared by treatment of PNGase F (New England Biolabs). Samples containing around 30 μg proteins was loaded to SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... cell and virus particle lysates were treated with PNGase F and Endo Hf (New England Biolabs) following the manufacturer’s protocol for 1 hour at 37°C prior to Western blotting.
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bioRxiv - Genomics 2023Quote: RNA was extracted from the cell lysates using the Monarch Total RNA Miniprep kit (NEB T2010) and its quality was spot-checked for random samples using the Bioanalyzer High Sensitivity RNA Kit (Agilent 5067-1513) ...
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bioRxiv - Molecular Biology 2019Quote: ... Heat mediated antigen retrieval pretreatment using 10 mM Sodium Citrate buffer (Vectors Biolabs) were used ...
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bioRxiv - Molecular Biology 2021Quote: Env solubilized from cell lysates and VLPs was denatured by boiling in denaturing buffer (New England Biolabs) for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: Cellular RNA was extracted from cell lysates using the Monarch Total RNA Miniprep Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Cleared cell lysates were incubated with PNGase F (500–1,000 U per 20 μl of lysats, NEB) for 30–45 min at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from cell lysates using the Monarch Total RNA Miniprep Kit (T2010S, New England BioLabs) according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2021Quote: MBP-Stwl antigen was purified from induced bacterial culture using Amylose Resin (NEB: E8021L). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were treated with PNGase F (NEB) following the manufacturer’s instructions or mock treated as a control ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were incubated with denaturing buffer (NEB) 95°C for 10 minutes and equal amounts of lysates (180 ug ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were incubated with amylose resin (NEB) at 4 °C for 1 h before beads were washed ...
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bioRxiv - Developmental Biology 2022Quote: ... PTH1R was deglycosylated by 1hour (secondary cell lines) or overnight incubation (primary cells or tissue lysate) with PNGaseF at 37°C (NEB, cat no, P0704S). 1µl of 1M DTT was added to 50µl of cell lysate to ensure complete dissociation of disulphide bonds linking cleaved extracellular domain of PTH1R ...
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bioRxiv - Microbiology 2022Quote: Cell lysates were prepared as described above used as DNA template for PCR amplification using Phusion polymerase (NEB) and oligonucleotides that anneal 150 bp outside of wzc ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were incubated with 0.9 mM MnCl2 and 7 U/μl lysate lambda protein phosphatase (λPP; New England BioLabs), or 0.9 mM MnCl2 and H2O as control ...
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bioRxiv - Cell Biology 2021Quote: ... the lysates were treated with endoglycosidase H (NEB).
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bioRxiv - Plant Biology 2021Quote: ... coli lysates were immobilized on amylose resins (NEB), incubated for 1 h at 4 °C with purified GST-XopS ...
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bioRxiv - Molecular Biology 2020Quote: ... lysate was first treated with DNAse I (NEB) to mitigate genomic DNA viscosity when loading into SDS-PAGE gels ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mg of cell lysate was cleared with protein A magnetic beads to remove non-specific interactions (S1425S, NEB) and incubated overnight with anti-ZAP antibody (Proteintech 16820-1-AP ...