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Citations for New England Biolabs :
651 - 700 of 10000+ citations for Cow Fibrinogen Like Protein 1 FGL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: The final microinjection mix contained 322 ng/μL spCas9 protein (New England Biolabs) with 40 ng/μL of each sgRNA and 127 ng/μL of the ssDNA repair template ...
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bioRxiv - Biophysics 2021Quote: ... pH 8.4 using cAMP-dependent protein kinase A (New England Biolabs, Frankfurt, Germany). The phosphorylated vimentin was mixed at the desired ratios of 1 ...
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bioRxiv - Cell Biology 2020Quote: All in vitro kinase assays were done in NEBuffer for protein kinase (NEB) at 30°C ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were affinity-purified using amylose resin using the manufacturer’s protocol (NEB) and eluted with 20 mM maltose ...
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bioRxiv - Biochemistry 2022Quote: ... Purified Vg was dephosphorylated with Lambda Protein Phosphatase (New England BioLabs, MA, USA). The samples were incubated with 1 µL Lambda Protein Phosphatase ...
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bioRxiv - Cell Biology 2021Quote: ... solubilized proteins were treated with Endo H and PNGase F (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... and PODXL2 were mixed with Protein Deglycosylation Mix II (New England Biolabs, #P6044S). The suggested protocol by the supplier was used with the exception of incubation time (the proteins were only incubated at RT for 20 h).
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bioRxiv - Plant Biology 2020Quote: ... Immunoprecipitation was performed using protein A magnetic beads (New England BioLabs, Ipswich, MA). Following the Proteinase K treatment of each sample ...
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bioRxiv - Microbiology 2021Quote: ... was incubated with 10 U/ul Lambda Protein Phosphatase(New England Biolabs, #P0753) at 30°C for 30 minutes ...
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bioRxiv - Cancer Biology 2019Quote: Purified hTERT_191-306 proteins were incubated with CDK1-cyclin B (New England Biolabs) or purified IKK2_2-664 proteins ...
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bioRxiv - Microbiology 2020Quote: ... Molecular weights were determined using protein standards (New England Biolabs P7712, or P7719). Puromycin incorporation assays were performed essentially as described in (38) ...
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bioRxiv - Cell Biology 2021Quote: ... and biotin-pupE labelled proteins were enriched by streptavidin magnetic beads (NEB, S1420S) and identified by mass spectrometry analysis (Liu et al. ...
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bioRxiv - Biochemistry 2020Quote: All proteins were expressed in Escherichia coli NiCo21 (DE3) cells (New England BioLabs). To co-express multiple proteins ...
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bioRxiv - Molecular Biology 2021Quote: ... The recombinant protein was sequentially purified using an amylose column (New England Biolabs) followed by a nickel-nitrilotriacetic acid column (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... ∼50 μg of protein were incubated with 20 units of λPP (NEB, USA) for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... following the final wash proteins were eluted in Glycoprotein Denaturing Buffer (NEB, P0702S) for 10 mins at 100°C and then resuspended in EndoH (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... 4.2 ng/μl extreme thermostable single-stranded DNA binding protein (New England Biolabs) and 0.02 U/μl SuperFi DNA polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... cross-linked virion samples were deglycosylated using Protein Deglycosylation Mix II (P6044, NEB) under denaturing conditions according to manufacturer’s instructions ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The MBP tagged proteins were purified by using Amylose Resin (New England Biolabs) and Ultrafiltration tubes (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... the REC114-MEI4 protein-containing supernatants were loaded onto an Amylose resin (NEB). After extensive washing with a buffer containing 20mM Tris pH 8 ...
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bioRxiv - Developmental Biology 2023Quote: ... The two gRNAs were injected together with Cas9-NLS protein (New England Biolabs) into one-cell stage embryos ...
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bioRxiv - Plant Biology 2023Quote: ... MBP-tagged and GST-tagged proteins were detected using anti-MBP (E8032S, NEB) and anti-GST antibody (60-021 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted by the addition of 1uL TEV Protease (New England Biolabs) and incubated for 2 hours at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein A magnetic beads (Cat#S1425S, New England Biolabs Inc, Ipswich, MA, USA) were aliquoted (20 □l ...
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bioRxiv - Developmental Biology 2023Quote: ... 2ng gRNA was injected together with 1.9nM Cas9 protein (New England Biolabs M0386T) and 10% Phenol Red (Sigma- Aldrich P0290 ...
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bioRxiv - Developmental Biology 2023Quote: ... Freshly laid wild-type TU-strain embryos were injected with SpCas9 protein (NEB) mixed with gRNAs (~300 ng/ul) ...
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bioRxiv - Biochemistry 2023Quote: ... and (MBP)2-HSPC300 proteins were purified through amylose beads (New England Biolabs). The Sra1/Nap1 dimer was obtained by co-expressing His6-Sra1 and untagged Nap1 in Tni insect cells (Expression Systems) ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was pre-cleared using protein G magnetic beads (New England Biolabs). The cleared supernatant was then incubated with 3 µg anti-myc antibody (SIGMA M4439 monoclonal anti c-myc or Abcam ab206486 rat mAb to myc tag (9E10) ...
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bioRxiv - Plant Biology 2023Quote: ... The MBP-fused protein was purified with Amylose Resin (E8021V; New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... either 1.8 mg USP2 or 800 U Lambda protein phosphatase (New England BioLabs) was added as indicated ...
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bioRxiv - Cell Biology 2024Quote: Proteins were expressed in Escherichia coli T7 Express lysY/Iqcells (New England Biolabs) in 2×TY medium at 37°C to an OD600 of 0.8–1.2 before inducing protein expression by addition of 0.4 mM isopropyl β-D-thiogalactopyranoside (IPTG) ...
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bioRxiv - Microbiology 2024Quote: ... His- tagged proteins were purified by NEBExpress® Ni-NTA Magnetic Beads (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... the protein-bound RNA was dephosphorylated with Quick CIP (M0525S, New England Biolabs) for 10 min in 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted proteins were loaded onto a 6 mL amylose column (New England Biolabs), washed in 50 mM HEPES-KOH (pH 7.5) ...
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bioRxiv - Synthetic Biology 2024Quote: All protein expression constructs (Supplementary Table S1) were cloned using Gibson assembly (NEB, where double-stranded DNA fragments are either ordered from IDT as gBlocks or PCR amplified from existing constructs with at least 25-bp overlap in sequence ...
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bioRxiv - Biochemistry 2024Quote: ... the protein bound RNA was dephosphorylated with Quick CIP (M0525S, New England Biolabs) for 10 min at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... GAL4-Creb5(1-128)T59/T61A and GAL4-Creb5(1-128)C18/C23S were generated by inducing point mutations in the GAL4-Creb5(1-128) vector using the Q5® Site-Directed Mutagenesis Kit (NEB, Cat#: E0554S). The control vector GAL4(1-147 ...
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bioRxiv - Microbiology 2020Quote: ... amplified with the primers Dhm1275-1 and −2 (Table 2) using a NEBuilder HiFi DNA Assembly Kit (New England BioLabs, Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 – 1 μg DNA template were in vitro transcribed using HiScribe® T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA), following manufacturer’s instruction ...
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bioRxiv - Genetics 2023Quote: ... 1 μg of RNA was used to generate sequencing libraries using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following polyA selection ...
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bioRxiv - Genomics 2023Quote: ... and single colonies were picked 1 day later to grow up and extract plasmid DNA using a Monarch Plasmid Miniprep Kit (New England Biolabs, Cat. No. T1010L). Extracted plasmid DNA was sequence confirmed via long-read Nanopore sequencing (Primordium Labs ...
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bioRxiv - Genomics 2023Quote: ... The cDNA libraries were constructed from 1 ug input RNA using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB), following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... Adaptor ligation was performed with 1:25 diluted adaptor and 15 cycles were used for library amplification using dual indices (NEB dual index kit). Paired-end 2×25 bp sequencing was performed on a NextSeq500 Illumina Sequencer.
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bioRxiv - Cell Biology 2023Quote: ... Adaptor ligation was performed with 1:25 diluted adaptor and 15 cycles were used for library amplification using dual indices (NEB dual index kit). Paired-end 2×25 bp sequencing was performed on a NextSeq500 Illumina Sequencer.
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bioRxiv - Cancer Biology 2023Quote: ... The sequencing libraries were prepared following Chapter 1 of the NEB® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs, NEB) protocol ...
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bioRxiv - Genomics 2023Quote: ... We then ligated barcodes to the end-prepped DNA using the Native Barcoding Expansion 1–12 kit (EXP-NBD104, Oxford Nanopore Technologies, Oxford, UK) and Blunt/TA Ligase Master Mix (New England Biolabs, Ipswich, MA, USA). We cleaned the barcoded samples using 0.9X AMPure XP beads ...
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bioRxiv - Genomics 2023Quote: ... using the Adapter Mix II from the Native Barcoding Expansion 1–12 kit and NEBNext Quick Ligation Reaction Buffer (New England Biolabs, Ipswich, MA, USA) as well as Quick T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Evolutionary Biology 2024Quote: We prepared dual indexed libraries according to the instruction manual using the NEBNext Ultra II DNA Library Prep Kit for Illumina and the NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1, New England Biolabs, Ipswich, Massachusetts, USA) and following the recommended conditions of bead-based size selection according to distribution of DNA fragments per sample ...
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bioRxiv - Genomics 2021Quote: ... using the Golden Gate assembly kit (NEB® Golden Gate Assembly Kit #E1601). Each guide sequence was cloned with its own U6 promoter and was followed by a sgRNA scaffold ...
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bioRxiv - Microbiology 2023Quote: ... A RNA cleanup kit (Monarch RNA Cleanup Kit T2050, New England Biolabs, France) was used to further clean the RNA samples ...