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Citations for New England Biolabs :
1 - 50 of 244 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... 8 μL of solution A and 6 μL of solution B were mixed with 16 U RNase Inhibitor (NEB), gBlock templates (2 nM each ...
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bioRxiv - Molecular Biology 2024Quote: ... containing a hygromycin resistance cassette using BamHI-HF and NotI-HF (NEB) restriction enzymes ...
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bioRxiv - Genetics 2024Quote: ... The cell suspension was subsequently treated with an equal amount of Solution B containing 100 IU/mL of DNase I (New England Biolabs), 0.5% BSA (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: The standard N-glycoprotein bovine pancreatic ribonuclease B (RNase B, New England Biolabs, USA) was used as a substrate to measure NGLY1 activity ...
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bioRxiv - Biophysics 2021Quote: ... coli B ER2566 from NEB; ampicillin ...
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bioRxiv - Biochemistry 2021Quote: ... bovine RNase B and fetuin (New England Biolabs) by PNGase F (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... into the HindIII and SpeI restriction sites of the pSEVA23a1 vector79 where a hygromycin resistance cassette had been inserted in BamHI and XbaI (all restriction enzymes were from New England Biolabs). Correct cloning was confirmed by Sanger sequencing (Eurofins) ...
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bioRxiv - Microbiology 2020Quote: ... B are ligated first by T4 DNA ligase (NEB) in 40μl system ...
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bioRxiv - Immunology 2023Quote: ... 1μl Cas9 (diluted 1:3 in diluent B, NEB), 1μl water (water was replaced with 100ng/μl trib1 RNA for cop1 experiments) ...
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bioRxiv - Immunology 2024Quote: ... using BsmBI (New England Biolabs, supporting table I-b). The plasmid containing the gRNA targeting mSppl3 or mUgcg (lentiCRISPR-v2-mSppl3gRNA-GFP or lentiCRISPR-v2-mUgcggRNA-GFP ...
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bioRxiv - Genomics 2024Quote: ... (b) PURExpress T7 recombinant IVT system (NEB Cat.#E6800L), for inserts in pTH6838 ...
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bioRxiv - Genetics 2020Quote: ... and cloned it into a lentiviral transfer pLEX vector carrying the hygromycin resistance gene using Gibson Assembly Master Mix (NEB E2611L). A 2A epitope tag was added to hACE2 at the C-terminus ...
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bioRxiv - Developmental Biology 2021Quote: ... IRES-Puro and an FRT-flanked PGK-hygromycin selection cassette to generate the targeting vector using the NEBuilder High-Fidelity DNA Assembly Cloning Kit (NEB, E5520). Twenty-one bp downstream of the start codon ...
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bioRxiv - Plant Biology 2024Quote: ... Flanking sequences and the hygromycin resistance gene were amplified from either genomic DNA or from plasmid pCHYG and using Phusion polymerase (NEB, UK). Fragments were gel purified using QIAquick Gel Extraction Kit (QIAGEN ...
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bioRxiv - Biochemistry 2021Quote: ... 125 to 75 U/ 5 μg RNase B (Cat.P7817S, NEB) and fetuin (Cat ...
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bioRxiv - Molecular Biology 2020Quote: ... Cas9 protein (IDT) was suspended with a Diluent B (NEB) to make 1 μM solution ...
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bioRxiv - Biochemistry 2022Quote: ... RNase B from bovine pancreas (New England Biolabs, Ipswich MA, USA), and horse radish peroxidase (HRP ...
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bioRxiv - Microbiology 2024Quote: ... coli B strain was dephosphorylated with 0.4 U/µL QuickCIP (NEB) in 1 x rCutSmart buffer (NEB ...
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bioRxiv - Microbiology 2024Quote: ... (b) Heat labile Shrimp Alkaline Phosphatase (rSAP) (1 µl) (NEB #M0371S) was used to remove terminal phosphates that could interfere with terminal extension by incubating in rCutSmart buffer (3.5 µl ...
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bioRxiv - Cell Biology 2023Quote: ... and one in-solution (NEB) DNase digestions were performed ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 3.2 µL solution A (NEB), 1 µL factor mix (NEB) ...
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bioRxiv - Neuroscience 2020Quote: ... Cas9 was diluted to 7 μM with diluent buffer B (NEB, Ipswich USA) on arrival and stored at −20 °C ...
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bioRxiv - Cell Biology 2022Quote: ... B and C oligonucleotides were phosphorylated with T4 polynucleotide kinase (NEB, Cat #M0201L) according to the manufacturer recommendations ...
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bioRxiv - Plant Biology 2020Quote: ... and 30X Enzyme Activator Solution (NEB). Digestion was verified on a TapeStation 2200 with a D1000 ScreenTape ...
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bioRxiv - Immunology 2020Quote: ... and Ribonucleotide Solution Set (NEB, USA). During development phase ...
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bioRxiv - Immunology 2024Quote: ... Deoxynucleotide (dNTP) Solution Mix (N0447L, NEB); Dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2023Quote: ... and one in-solution DNase (NEB) digestions were performed ...
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bioRxiv - Cell Biology 2023Quote: ... The pet30-B vector was digested with Nde1 (R0111, New England BioLabs, Waltham, MA) and EcoRI-HF (R3101 New England BioLabs ...
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bioRxiv - Biochemistry 2020Quote: ... deoxynucleotide solution mix (dNTP, 2.5 mM, NEB) and Phusion HF Buffer (1x ...
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bioRxiv - Biochemistry 2020Quote: ... deoxynucleotide solution mix (dNTP, 2.5 mM, NEB) and Phusion HF Buffer (1x ...
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bioRxiv - Cell Biology 2022Quote: ... 1.25µl of EnGen Cas9 NLS solution (NEB), 1.75 µl H2O ...
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bioRxiv - Bioengineering 2023Quote: ... 1.4 mM dNTP solution (New England Biolabs), 1.6 μM FIP/BIP ...
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bioRxiv - Genetics 2020Quote: ... 2.5 μL of Taq Buffer B (Mg-free; 10X) (New England Biolabs, Ipswich, MA, USA), 2.5 μL of dNTPs (2.5 mM of each base) ...
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bioRxiv - Bioengineering 2023Quote: These inserts were then cloned into the pSECRETS-B cassette via USER cloning (NEB #M5505S). To maintain library diversity ...
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bioRxiv - Systems Biology 2021Quote: ... 0.4μL 10 mM dNTP Solution (New England Biolabs), 4μL 5x Phusion HF Buffer (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... 200 μM dNTP Solution Mix (New England Biolabs), and a concentration of biotin-11-dNTPs (PerkinElmer ...
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bioRxiv - Developmental Biology 2022Quote: ... 20μl of extraction solution (25μl of PK (NEB) /ml of NP40 lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: Proteinase solution: 4 μL proteinase K (NEB, P8107S) was added into 1 mL 0.1 M Tris-HCl 0.05 M EDTA (pH 8.0 ...
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bioRxiv - Biophysics 2023Quote: ... with the following: 3.2 μL solution A (NEB), 1 μL factor mix (NEB) ...
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bioRxiv - Cancer Biology 2023Quote: ... 2.5μl Deoxynucleotide (dNTP) Solution Mix (New England Biolabs), 0.5μl P5 primer pool ...
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bioRxiv - Genomics 2023Quote: ... 1.5 μL 1:1,249 diluted Fe2+ solution (NEB), and 8.4 μL water ...
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bioRxiv - Genomics 2023Quote: ... 3 μL 1:1,249 diluted Fe2+ solution (NEB)) at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μl dATP solution 100 mM (NEB; # N0440S), 1 μl BSA (20 mg/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 of α2-3,6,8,9 Neuraminidase A (New England Biolabs, Fig. 2d and Extended Data Fig. 2a,b) were added with 1.5 µL of 10× GlycoBuffer 1 (New England Biolabs ...
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bioRxiv - Systems Biology 2020Quote: ... 0.4 μL 10 mM dNTP Solution (New England Biolabs), 4 μL 5x Phusion HF Buffer (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... and deoxynucleotide solution (both from New England Biolabs, NEB). The resulting PCR product was integrated into the plasmid via the Gibson Assembly cloning system (New England Biolabs-NEB) ...
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bioRxiv - Microbiology 2022Quote: ... and deoxynucleotide solution (both from New England Biolabs, NEB). The resulting PCR product was integrated into the plasmid via the Gibson Assembly cloning system (New England Biolabs-NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... 2 uL dNTP Solution Mix (10 mM stock, NEB), 2 μl Taq DNA ligase (80 U ...
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bioRxiv - Immunology 2023Quote: ... and 25ul 10X DNaseI enzyme reaction solution (NEB, M0303L) were added and incubated at 37°C for 20 min ...
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bioRxiv - Systems Biology 2023Quote: ... 0.4 μL 10 mM dNTP solution (New England Biolabs), 4 μL 5× phusion HF buffer (Thermo Scientific) ...