Labshake search
Citations for New England Biolabs :
1 - 50 of 4933 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2022Quote: ... 5 mM EDTA) + 4 μl of Proteinase K (20 mg/ml, NEB) at 55°C (300 rpm continuous shaking in a thermomixer) ...
-
bioRxiv - Systems Biology 2020Quote: ... 22 μL of 10 mM/each of four dNTPs (NEB), 5.5 μL of TSO primer (100 μM) ...
-
bioRxiv - Genetics 2020Quote: ... We mixed 20 µg of insert or 15 µg plasmid library with 5 µL AscI (NEB R0558L), 5 µL SbfI-HF (NEB R3642L) ...
-
bioRxiv - Genomics 2020Quote: ... 1 μL of 10 mM dATP and 15 units of Klenow 3’ → 5’ exo- (NEB M0212L) was added to blunted ends and incubated at 37°C for 30 minutes ...
-
bioRxiv - Neuroscience 2022Quote: ... 15-20 µL of amylose beads (New England Biolabs) were mixed with (MBP)2-WRC (bait ...
-
bioRxiv - Microbiology 2022Quote: ... 15 μl 20% SDS and 3 μl 20 mg/ml Proteinase K (NEB #P8107) was added to the sample and the mixture was incubated at 37 °C for 30 min with agitation at 1400 rpm to completely lyze the cells ...
-
bioRxiv - Molecular Biology 2022Quote: ... and Phusion DNA polymerase after 20 cycles of amplification (1.2×10−5) (NEB, product literature). Because the error rate from the RNA polymerase was much higher than the RT and PCR steps ...
-
bioRxiv - Neuroscience 2024Quote: ... The UNC13A CE was amplified with a forward primer in exon 19 5’-CAGACGATCATTGAGGTGCG-3’and reverse primer in exon 22 5’-ATACTTGGAGGAGAGGCAGG-3’using Q5 High Fidelity Master Mix (NEB). PCR products were resolved on a TapeStation 4200 (Agilent ...
-
bioRxiv - Synthetic Biology 2024Quote: ... All reactions were mixed to 22 µl including 10 U of HindIII-HF (NEB, Catalog #R3104L) and up to 2 µl of QuickExtract lysates ...
-
bioRxiv - Molecular Biology 2021Quote: ... 5 µL ExoI (20 U/µL, NEB), 7 µL HinFI (10U/µL ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 15 units of Klenow Fragment (3’→5’ exo-, NEB). Following extension reactions ...
-
bioRxiv - Genomics 2024Quote: ... 2 μL of 10% Tween-20 and 5 μL NEBNext High-fidelity 2× PCR Master Mix (NEB, M0541) was added to each well sequentially ...
-
bioRxiv - Cancer Biology 2021Quote: ... 10% NP-40 (20 μL, NEB), G7 Reaction buffer (20 μL ...
-
bioRxiv - Biochemistry 2023Quote: ... 15% PEG8000 and 20 U T4 RNA ligase 2 truncated KQ (NEB)) ...
-
bioRxiv - Neuroscience 2020Quote: ... either purified directly (insert) or from agarose gel (vector) and ligated (16-18 h, 20-22 °C) with T4 DNA ligase (NEB) according to the manufacturer’s protocol.
-
bioRxiv - Genomics 2023Quote: ... followed by the addition of 22 µL of ligation mix (20 µL quick ligase buffer and 2 µL of quick ligase, NEB) and incubation at 25°C for 10 minutes ...
-
bioRxiv - Systems Biology 2023Quote: ... 22 μL of a mixture containing 10 mM of each of the four deoxynucleotide triphosphates (dNTPs) (NEB), 5.5 μL of TSO primer (100 μM) ...
-
bioRxiv - Microbiology 2023Quote: ... 5 μL 20 μM Cas9 (New England BioLabs), and 5 μL of 40 μM transcribed dgRNAs ...
-
bioRxiv - Cell Biology 2023Quote: ... Klenow fragment (5 U) & T4 polynucleotide kinase (15 U) (NEB; M0201). The A-tailing was performed with Klenow exo-fragment (15 U ...
-
bioRxiv - Genomics 2020Quote: ... followed by addition of 20 μl Ligation master mix (containing: 15 μl NEB Quick ligation buffer ...
-
bioRxiv - Genomics 2020Quote: ... 15 mM HEPES pH 8) and 20 μl of DNase I (M0303S, NEB). The Nuclease Flushing mix was loaded into the flow cell and incubated for 30 min ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 10 or 20 mM of NH4SO4) (NEB), 0.625 ng/µL of purified DNAP ...
-
bioRxiv - Developmental Biology 2021Quote: ... PCR amplification was run for 15 cycles and NEBNext Index1-24 primers for Illumina were used (New England Biolabs).
-
bioRxiv - Genomics 2023Quote: ... or 40,000 nuclei per well in 22 µL of NSB and 2 µL of 10 mM dNTP mix (NEB).
-
bioRxiv - Molecular Biology 2024Quote: ... 5 μl 4× Template Switching RT buffer (NEB), 1 µl of 75 μM T7-TSO (5’-/5Biosg/ACTCTAATACGACTCACTATAGGGAGAGGGCrGrGrG-3’) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 15 µL of labeling mix (4 µL 10X ThermoPol Reaction buffer (NEB, B9004S), 2 µL of 5 mM aa-dUTP/dNTP mix (5 mM each dATP ...
-
bioRxiv - Genomics 2022Quote: ... and resolved on a 4–20% SDS-polyacrylamide gel (NEB). The presence of MeCP2 was assayed by western blotting using anti-MeCP2 monoclonal antibody M6818 (Sigma ...
-
bioRxiv - Genomics 2020Quote: ... 10 µ of 20 mg/mL BSA (NEB), and 950 µ of nuclease-free water ...
-
bioRxiv - Biochemistry 2021Quote: ... 5 nM tritiated pSP1 was nicked either using 50 nM Cas12a RNP (WT protein with crRNA 24) at 25°C in Buffer RB for 10 s or using 10 units Nt.BspQI (New England Biolabs) per µg DNA ...
-
bioRxiv - Plant Biology 2020Quote: ... Library preparation began with digestion of 100 ng cleaned genomic DNA at 37°C for 4 h in 15 μL containing 4 U FspEI (NEB), 1X CutSmart Buffer (NEB) ...
-
bioRxiv - Plant Biology 2023Quote: ... 5% (v/v) BsaI at 20 U/μL (NEB #R3733), 10% (v/v ...
-
Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... we performed 2 reactions per sample (20 µl 5× NEB Q5 buffer ...
-
bioRxiv - Bioengineering 2024Quote: ... The plasmid pool was then digested with the outer guides M1 and P1 (5 μg plasmid pool, 10 μL M1+P1 Cas9 RNP, 4 μL 10X rCutSmart buffer (NEB, B6004S), and H2O to 40 μL incubated at 37°C for 1 hour) ...
-
bioRxiv - Cell Biology 2022Quote: ... 4 μL of 20 mg/mL Proteinase K (New England BioLabs) was added and incubated for 1 h at 55 °C ...
-
bioRxiv - Bioengineering 2022Quote: ... 4 μL of 20 mg/mL Proteinase K (New England BioLabs) was added and incubated for 1 hours at 55°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... 4 µL of 20 mg/mL Proteinase K (New England BioLabs) was added and incubated for 1 h at 55 °C ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5 μl 10× blunting buffer (NEB; # B1201SVIAL), 5 μl dNTP 1 mM (NEB ...
-
bioRxiv - Biochemistry 2020Quote: ... 20 μl of 10 mM dNTPs (New England Biolabs), 10 μl of Sulfolobus DNA polymerase IV (New England Biolabs ...
-
Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... 20 µl phi29 DNA Polymerase (10 U/µl, NEB) and incubated for 12 h at 30 °C ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10 µl of 20 mg/ml RNase A (NEB) was added ...
-
bioRxiv - Genetics 2024Quote: ... 10 µL Proteinase K (NEB #P8107, 20 mg/ml), and 10 µL 1M DTT (GoldBio #DTT in dH2O ...
-
bioRxiv - Cell Biology 2024Quote: ... 4 µL 10 mM ATP (NEB, Cat #P0756S), 40U (2 µL ...
-
bioRxiv - Molecular Biology 2021Quote: ... 5’ ends were phosphorylated by 20 U polynucleotide kinase (PNK; NEB) by adding 1 mM ATP (Thermo Scientific) ...
-
bioRxiv - Molecular Biology 2020Quote: 5’ end phosphorylation (1x PNK buffer, 20 u T4 PNK (NEB), 80 u RNaseOUT ...
-
bioRxiv - Genetics 2024Quote: ... An additional 20-minute digestion was then performed with 5 µL RNase A (NEB #T3018, 20 mg/ml). The rest of the protocol remained unchanged ...
-
bioRxiv - Genomics 2020Quote: ... 4 μL of 20 mg/mL RNase A (New England Biolabs #T3018L) is added to the RNase positive aliquot of non-lysed TSB from 1-gram cannabis flower homogenate ...
-
bioRxiv - Genomics 2021Quote: ... and 4 μl 5 U/μL I-SceI (NEB, #R0694L) in a 50 μL final volume for 3 hours at 37°C ...
-
bioRxiv - Genomics 2020Quote: ... 10 µ of 20 mg/mL BSA (New England BioLabs), and 10 µ of nuclease-free water ...
-
bioRxiv - Microbiology 2021Quote: ... 10 μl of proteinase-K (NEB, stock 20 mg/ml) was added and incubated at 37°C for 1 hour ...
-
bioRxiv - Microbiology 2024Quote: ... 10 µl of proteinase-K (NEB, stock 20 mg/ml) was added and incubated at 37 ℃ for 1 h ...