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Citations for New England Biolabs :
151 - 200 of 3275 citations for Collagen 17 alpha 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The following cloning strains were used: NEB Stable (lentiviral and piggybac vectors) and NEB 5-alpha (all other plasmids) (New England Biolabs). For cloning of base editor constructs ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids carrying the dcas9/lacI cassette were recovered and propagated in the NEB 5-alpha F’ Iq strain (New England Biolabs). Cloning and/or propagation of plasmids containing the dcas9/lacI cassette in host E ...
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bioRxiv - Molecular Biology 2022Quote: ... following the manufacture’s protocol. Plasmids were transformed in DH5-alpha or DH10-beta chemo-competent Escherichia coli (E. coli) cells (New England Biolabs). Transformed bacteria were grown in LB medium supplemented with 50 μg/mL kanamycin or 100 μg/mL carbenicillin ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 μl of the assembly reaction was transformed into 5 μl chemically competent cells (E. coli NEB 5-alpha, New England Biolabs). Circuit constructs were analyzed by PCR ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was extracted from the heads of 20 male adults or 17 female adults using a Monarch Total RNA Miniprep Kit (NEB). 3 replicates for male and 3 replicates for female flies were done for each genotype ...
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bioRxiv - Microbiology 2020Quote: ... Circularised product is subjected to two rounds of rRNA depletion using biotinylated oligos [17] and streptavidin-coated magnetic beads (NEB) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The rRNA-subtracted circular product is subjected to a final round of PCR amplification with Illumina adaptor primers [17] using Phusion polymerase (NEB). All the libraries were then quantified ...
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bioRxiv - Cell Biology 2023Quote: ... sgRNA cassettes were isolated by two rounds of PCR using a previously published strategy (17) with NEBNext Ultra II Q5 Master Mix (New England Biolabs) and primer pairs listed in Extended Data Table 2.
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bioRxiv - Immunology 2022Quote: ... N-glycans were enzymatically removed from the underlying peptides with PNGase F (NEB) overnight at 37 °C ...
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bioRxiv - Immunology 2019Quote: ... A 7-mer random peptide library (E8100S, Ph.D. -7, New England Biolabs, USA) was panned overnight at 4°C on pooled human IgM adsorbed on polystyrene plates at a concentration of 0.1 mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... The Peptide-N-Glycosidase F (PNGase F) enzyme (New England Biolabs, catalog #: P0704L)-treated samples served as a control for unglycosylated α1 subunits ...
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bioRxiv - Immunology 2023Quote: ... N-glycans were enzymatically removed from the tryptic peptides with PNGase F (NEB) overnight at 37°C ...
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bioRxiv - Genetics 2019Quote: ... coli was performed using DH5-alpha or its commercial derivative NEB 10-Beta (New England Biolabs product number C3019I or C3020). Genomic DNA of BY4741 ...
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bioRxiv - Microbiology 2019Quote: ... Colonies were selected using a gentamycin marker and blue/white colony screening in a background of dH5-alpha cells (New England Biolabs, UK). The resulting construct was transformed into PA14 and the clone used for assays was verified by Sanger sequencing (University of Sheffield ...
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bioRxiv - Bioengineering 2021Quote: ... dsDNA fragments were inserted into PCR-amplified (primers 17-18 in Table S3) and purified pRSET vector fragment by Gibson Assembly (NEB, USA) to add sequence parts necessary for cDNA display generation to the DNA library ...
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bioRxiv - Microbiology 2021Quote: ... the sgRNA-encoding region was amplified from 500 ng genomic DNA with 17 cycles of PCR using NEBNext Ultra II Q5 master Mix (NEB #M0544L). Each PCR reaction contained a pool of forward primers (0.5 μM final concentration ...
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bioRxiv - Microbiology 2022Quote: ... The sgRNA-encoding region was amplified from 500ng genomic DNA with 17 cycles of PCR using NEBNext Ultra II Q5 master mix (NEB M0544L) as described in ...
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bioRxiv - Genetics 2019Quote: ... DBT1 loci were first amplified with 17 cycles of PCR using a touchdown protocol and the NEBnext 2x master mix (New England Biolabs M0541). The resulting product served as input to a second PCR ...
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bioRxiv - Physiology 2020Quote: ... Mutagenesis of Ser125 to Arg in GPT and Ser153 to Arg in GPT2 17 was performed with the Q5 site-directed mutagenesis kit from NEB (#E0554). Adeno-associated viruses (AAV ...
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bioRxiv - Evolutionary Biology 2020Quote: Phage display experiments were performed using the PhD-12 peptide phage display kit (NEB). All steps involving the pipetting of phage-containing samples was done using filter tips (Rainin) ...
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bioRxiv - Biochemistry 2020Quote: ... tsetse saliva was treated with peptide-N-glycosidase A (PNGase A, New England Biolabs), which releases all N-linked glycans ...
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bioRxiv - Cell Biology 2019Quote: ... The PfGRP170 transit peptide PCR was digested with Nhe1 and AatII (New England Biolabs) and the GFP PCR was digested with AatII and BglII (New England Biolabs) ...
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bioRxiv - Neuroscience 2021Quote: ... Deglycosylation was performed with peptide-N-glycosidase F (PNGase F; New England Biolabs, USA) following the manufacturer’s instructions [28] ...
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bioRxiv - Neuroscience 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabsⓇ Inc., MA, USA) was preferred in order to select aggregation inhibitory peptide selection ...
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bioRxiv - Bioengineering 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabs□ Inc., MA, USA) was preferred ...
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bioRxiv - Bioengineering 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabs□ Inc., MA, USA) was used ...
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bioRxiv - Cell Biology 2022Quote: ... and transformed into competent DH5a E. coli (5-alpha Competent E. coli) following the protocol provided by the company (New England Biolabs, Ipswich, MA). For colony selection ...
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bioRxiv - Cell Biology 2021Quote: ... 1 uL of the Gibson Assembly reaction mixture was transformed via heat shock into chemically competent NEB 5-alpha cells (NEB, Catalog #C2988J), and cells were incubated on lysogeny broth with 10 μg/mL tetracycline (LB-Tet10 ...
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bioRxiv - Microbiology 2021Quote: ... The amplicon was cloned into the pTXTL-T7p14-aH plasmid (replacing alpha hemolysin, Daicel Arbor Biosciences, Ann-Arbor, MI) using NcoI-HF and SmaI (New England Biolabs, Ipswitch, MA). The new plasmid construct (pSLP15 ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 μl of the assembly reaction was transformed into 5 μl chemically competent cells (E. coli NEB 5-alpha, New England Biolabs). Circuit constructs were analyzed by PCR ...
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bioRxiv - Cell Biology 2022Quote: ... End-repair/A-tailing was performed on 17 μL of ChIPed DNA using NEBNext® Ultra™ II End Repair/dA-Tailing Module (New England BioLabs), followed by ligation (MNase_F/MNase_R ...
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bioRxiv - Microbiology 2020Quote: ... pCrPV-1A-DcDV was linearized by inverse PCR with primers 17 and 18 and the linearized plasmid was circularized by blunt end ligation with T4 DNA ligase (New England Biolabs, Ipswich, Massachusetts) according to the manufacturer’s instructions to create pCrPV-1A-DcDV-1A ...
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bioRxiv - Bioengineering 2022Quote: ... End-repair/A-tailing was performed on 17 μL of ChIPed DNA using NEBNext® Ultra™ II End Repair/dA-Tailing Module (New England BioLabs), followed by ligation (MNase_F/MNase_R ...
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bioRxiv - Molecular Biology 2022Quote: ... end-repair/A-tailing was performed on 17 µL of ChIPed DNA using NEBNext® Ultra™ II End Repair/dA-Tailing Module (New England BioLabs), followed by adapter ligation using T4 DNA Ligase (New England BioLabs) ...
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bioRxiv - Immunology 2020Quote: ... We ran the genotyping PCR for the Rosa26 and the collagen status with the Phusion® High Fidelity DNA Polymerase (New England Biolabs) and the same primers as described previously and indicated in the Supplementary Table 2 [6].
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bioRxiv - Biochemistry 2020Quote: Tsetse salivary proteins were treated with peptide-N-glycosidase F (PNGase F, New England Biolabs), which cleaves all N-linked glycans except those with an α-1,3 fucose modification of the chitobiose core [20] ...
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bioRxiv - Neuroscience 2019Quote: ... protein samples were treated with peptide-N-Glycosidase F (PNGase) (New England Biolabs, Whitby, ON) following manufacturers guidelines ...
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bioRxiv - Neuroscience 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabs® Inc., MA, USA) was amplified as following the recommended protocols by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... to prevent exoIII digestion on the arrest peptide side and then with AvrII (NEB, R0174S) to allow for exoIII digestion towards the candidate protein ...
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bioRxiv - Evolutionary Biology 2023Quote: ... g165 and g166 without the predicted signal peptides were PCR-amplified with Phusion polymerase (NEB) from genomic DNA of P ...
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bioRxiv - Genomics 2021Quote: ... Cells were centrifuged and resuspended in 600 μl Blocking buffer (PBS + 2 % BSA (B9000S, New England Biolabs)) and incubated for 5 min on ice ...
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bioRxiv - Biochemistry 2019Quote: ... we used a commercial assay kit (Ph.D.-7 Phage Display Peptide Library Kit, New England Biolabs) and followed the recommended protocol for “solution phase panning with affinity bead capture” with the following modifications ...
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bioRxiv - Synthetic Biology 2021Quote: ... DNA templates for MS-QBiC peptide synthesis were amplified by PCR using Taq DNA polymerase (NEB) with a T7 promoter primer (5′-GGGCCTAATACGACTCACTATAG-3′ ...
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bioRxiv - Biochemistry 2020Quote: ... Desalted peptides were dissolved at a concentration of 3 µg/µL in 1x CutSmart buffer (NEB, 50 mM potassium acetate ...
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bioRxiv - Cell Biology 2022Quote: ... enzyme digestion or Peptide-N-Glycosidase F (PNGase F) (New England Biolabs, Ipswich, MA, catalog #: P0704L) enzyme digestion was performed according to manufacturer’s instruction and the published procedure [49].
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Systematic Analysis of Lysine Succinylation in Vero cells infected with Small Ruminant MorbillivirusbioRxiv - Genomics 2021Quote: Lysine-succinylated peptides were enriched using agarose-conjugated pan anti-succinyllysine antibody (PTM Biolabs, Hangzhou, China). In brief ...
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bioRxiv - Genetics 2023Quote: ... following which they were incubated in blocking buffer composed of 0.5 mg/ml BSA (New England Biolabs, #B9001S), 80 units of RNAse-OUT RNAse inhibitor (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... pUHES:UmPit2+SP:mCherry with its natural signal peptide was generated via NEBuilder HiFi DNA Assembly Cloning Kit (NEB) by combining PCR-amplified UmPit2+SP:mCherry using primers 2127 + 2128 and PCR-amplified pUHESdest using primers 2125 + 2126 without UhAvr1 signal peptide ...
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bioRxiv - Cell Biology 2020Quote: ... These peptides were used as the substrate in a PKA kinase assay kit (New England Biolabs, #P6000S) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... These peptides were used as the substrate in a PKA kinase assay kit (New England Biolabs, #P6000S) according to the manufacturer’s instructions ...