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Citations for New England Biolabs :
351 - 400 of 1180 citations for Chloroethane D5 98% 1000 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 0.2U/mL RNase Inhibitor (NEB, M0314), Complete protease inhibitor cocktail (Roche ...
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bioRxiv - Genomics 2024Quote: ... 200 µg/mL rAlbumin (New England Biolabs B9200S), and 1 U/µL Phi29 DNA polymerase (Thermo Fisher Scientific EP0091) ...
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bioRxiv - Genomics 2024Quote: ... 200 µg/mL rAlbumin (New England Biolabs B9200S), 0.4 U/µL RNase H (Enzymatics Y9220L) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.5 µL BsaI-HFv2 (NEB, 20,000 U/mL) and 0.5 µL T4 DNA Ligase (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.025 U/ml Taq polymerase (NEB, #M0267) in a final reaction volume of 15 µl and published thermal cycling parameters [17] ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1 mg/ml recombinant albumin (New England Biolabs), 0.1 mg/ml RNase A (QIAGEN) ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1 mg/ml recombinant albumin (New England Biolabs), and 0.1 mg/ml RNase A (QIAGEN)] ...
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bioRxiv - Biochemistry 2023Quote: ... 50 μg/ml BSA (New England Biolabs, Inc.), 0.005% Triton X-100 ...
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bioRxiv - Developmental Biology 2020Quote: ... mRNA was enriched from 1000 ng of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and 6 cDNA libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Genomics 2022Quote: ... The particles-tethered-magnetic-beads (hereafter referred to as ‘P_beads’) were then either digested with 1000 U of HindIII-HF (NEB) in 1 ml of WB1 at 37 °C with mild rotation for over 6 hrs ...
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bioRxiv - Neuroscience 2019Quote: ... PBT was removed and samples were incubated in SNAP substrate diluted in PBT (SNAP-Surface649, NEB S9159S; 1:1000) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The DNA was then digested using 1000 units of either MluCI or HpaII in 1× NEB1 digestion buffer (NEB) and 1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... 1000 ng of total RNA were used for mRNA enrichment by using NEBNext mRNA polyA Isolation Module (NEB, #E7490L), and libraries for Illumina sequencing were performed using the NEBNext Ultra II RNA kit (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... 50 mM Tris-Cl pH 7.4, 12 mM MgCl2, 1% NP-40, 0.5 mM DTT, 0.1 mg/ml cycloheximide, 80 U/ml NEB murine RNase inhibitor). Beads were transferred to a fresh tube and immunoprecipitated RNA extracted by incubating beads in buffer RLT (Qiagen ...
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bioRxiv - Developmental Biology 2022Quote: aPSM and pPSM specimens were separately diced and incubated with proteinase K (10μL at 20mg/mL U/mL, New England Biolabs, Ipswich, MA) at 37 °C - overnight to digest protein components ...
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bioRxiv - Biophysics 2022Quote: ... A low-pressure chromatography column connected to a peristaltic pump (5 mL/min flow rate) was prepared with 5 mL amylose resin (NEB #E8021S), followed by equilibration with 50 mL Amylose A Buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The plugs were washed in 20 ml of Milli-Q water for 30 minutes and then 10 ml of 1x rCutSmart buffer (New England Biolabs, B6004S) for 1 hour followed by a further hour in 5 ml of 1x rCutSmart buffer ...
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bioRxiv - Genomics 2020Quote: ... Libraries were prepared from 1000 ng total RNA using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) by the Transcriptional Regulation and Expression Facility at Cornell University.
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bioRxiv - Biochemistry 2019Quote: ... The pellet was resuspended in 28 µl hypertonic buffer and incubated at room temperature for 15 min with 1000 gel units of micrococcal nuclease (NEB). Chromatin-bound proteins were released from the DNA by addition of 7 µl of 1 M ammonium sulfate on ice for 5 minutes ...
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bioRxiv - Plant Biology 2019Quote: ... 1000 ng of extracted RNA was used as a template for cDNA synthesis using MMLV-reverse transcriptase (New England Biolabs) and random hexamers to prime cDNA synthesis ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we added 150 – 1000 ng of DNA to 1 μl of prepared MBD2-Fc/protein A beads (New England Biolabs), then washed and eluted the DNA as described by Chiou and Bergey (2018) ...
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bioRxiv - Genetics 2020Quote: ... A 1000 pmol sample of PAGE-purified sgRNA was treated with 20 U of calf intestine phosphatase (M0525L; New England BioLabs) at 37°C for 3 h to remove the 5’ phosphate group to prevent triggering innate immune responses(35) ...
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bioRxiv - Neuroscience 2020Quote: ... + 5 % non-fat milk for 1 hour at room temperature before incubating with primary antibody: anti-GFP (1:1000, Biolabs) or anti-actin (1:5000 ...
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bioRxiv - Plant Biology 2023Quote: ... from 100 to 1000 ng of total RNA using a stranded kit (Illumina TruSeq Stranded Total RNA Kit or NEB Next Ultra™ II Directional RNA Library Prep Kit for Illumina) ...
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bioRxiv - Molecular Biology 2023Quote: ... heated to 95°C and slowly cooled to 55°C and put on ice before the addition of 1000 units of T4 DNA ligase in 1x buffer (New England Biolabs (NEB)) and 10 mM dithiothreitol and incubated at room temperature overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Receptors were labelled both at the surface and intracellularly by addiction of the cell permeable SNAP-Cell 647 SiR ligand (1:1000, New England Biolabs) in DMEM F12 without serum and phenol red (21041-025 ...
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bioRxiv - Neuroscience 2023Quote: ... libraries were constructed from 1000 ng total RNA (NEBNext Multiplex Small RNA library prep set for Illumina, NEB-E7560S, New England Biolabs) and the small RNA fraction sequenced (NextSeq 500/550 High Output Kit v2.5 75 Cycles ...
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bioRxiv - Genetics 2023Quote: ... and SWI4 3’UTR (1000 bases downstream of ORF) were cloned into a LEU2 single integration vector by Gibson assembly (NEB) (Gibson et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... adding nuclease-free water to a final volume of 1 L) with 1:1000 (vol/vol) proteinase K (New England Biolabs) at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were treated with RNAse (100 μg/ml) at room temperature for 15 min and then with proteinase K (825 μg/ml, NEB™, P8107S) for 1 hour at 65°C ...
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bioRxiv - Genetics 2021Quote: ... and 0.1 mg/ml Proteinase K (New England Biolabs). DNA was electrophoresed in 1.2% agarose gels in 1xTAE (Tris-acetate-EDTA ...
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bioRxiv - Biochemistry 2021Quote: ... Supernatant was incubated with 0.75 mL amylose resin (NEB) for 2 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... and 0.1 mg/ml Proteinase K (New England Biolabs) and incubated at 37 °C for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 700µl G2 and 50µl Proteinase K (800U/ml, NEB) was added to each tube and incubated at 50°C for 2h with occasional tube inversions ...
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bioRxiv - Genomics 2020Quote: ... One microlitre of T4 DNA ligase (NEB, 400,000U/mL) was added to the reaction and incubated at 16 °C to ligate inserts ...
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bioRxiv - Genomics 2020Quote: ... 0.25 μl SbfI-HiFi (NEB 20,000 U/ml R3642L) and 4 μl of 10X cutsmart buffer ...
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bioRxiv - Genomics 2020Quote: ... 0.5 μl T4 Ligase (NEB, 400,000 U/ml m0202L), 6 μl of 10X T4 ligase buffer and 11.5μl water were added directly to the digested DNA ...
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bioRxiv - Genomics 2020Quote: ... using 0.25 μl MspI (NEB, 20,000 U/ml R0106S), 0.25 μl SbfI-HiFi (NEB 20,000 U/ml R3642L ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.5 µL 1 mg/mL bovine serum albumin (NEB), 0.25 µL T4 DNA ligase at 400 U/µL (NEB) ...
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bioRxiv - Cancer Biology 2019Quote: ... 8 μL of BSA (20 mg/mL B9000S, NEB,) and 100 Units of ligase (M0202M ...
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bioRxiv - Microbiology 2020Quote: ... A DNase I RNAse free 2000 U/ml (NEB) treatment was done using 1 μl for 15 minutes at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in 5 ml 1x NEB Buffer 2 (NEB) and drop frozen in liquid nitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... 0.04 units/mL DNase I (New England Biolabs, Inc.) pH 7.5-8.0) ...
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bioRxiv - Molecular Biology 2020Quote: 5 μl Bst 3.0 (NEB, M0374L, 8,000 units/ml),
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bioRxiv - Biophysics 2021Quote: ... 0.2 unit/mL yeast inorganic pyrophosphatase (New England Biolabs) [28] ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μL of 5000 units/mL RNaseH (NEB, M0297L) was used per µg of DNA and incubated for 48 hours at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... and RNasin (New England BioLabs UK, 100 U/mL).16 For nested PCR experiments ...
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bioRxiv - Genomics 2021Quote: ... 1 μL of Endonuclease IV (10,000 U/ml, NEB) was added to the reaction ...
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bioRxiv - Microbiology 2020Quote: ... 1 µL of T4 DNA ligase (400,000U/mL, NEB) and 2 µL ddH2O ...
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bioRxiv - Microbiology 2020Quote: ... packed with 2 ml amylose resin (New England Biolabs) equilibrated with buffer AF ...