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Citations for New England Biolabs :
1 - 50 of 5364 citations for Carbamic acid 2 4 hexadienyl 1 1 dimethylethyl ester E E 9ci since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: The recombinant constructs (pETDuet-1-TaPHB-1 and pETDuet-1-TaPHB-1-RUVBL1 were separately transformed into E. coli Lemo21(DE3) (NEB) chemical competent cells ...
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bioRxiv - Cancer Biology 2020Quote: ... and E-cadherin (24E10, Cell Signaling Technology, NEB, Frankfurt, Germany, #3195, 1:400) were used for immunohistochemistry (IHC ...
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bioRxiv - Cancer Biology 2022Quote: ... AAK1(S-676D/E/A) and UVRAG (T-518D/E/A) using Q5-site directed mutagenesis Kit (NEB). Manufacturer’s instructions for mutagenic primer design were followed ...
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bioRxiv - Molecular Biology 2024Quote: Plasmid pHERD30t with and without overlapping AluI/BREX site was purified from BREX+ (E. coli HS) or BREX- cells (E. coli BW25113) using Monarch Plasmid Purification Kit (NEB). 100 ng of plasmid was restricted with AluI (ThermoScientific ...
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bioRxiv - Neuroscience 2020Quote: ... 0.09 μL DNA ligase (E. coli) (NEB, M0205) and 0.09 μL Ambion RNase H (E ...
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bioRxiv - Microbiology 2021Quote: ... T7 SHuffle (C3026, E. coli K strain, NEB) and Nico (λDE3 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5ul DNA ligase (E. coli, NEB cat. M0205S), 2ul DNA polymerase I (E.coli ...
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bioRxiv - Genetics 2024Quote: ... coli (NEB® 5-alpha Competent E. coli), and successful genetic manipulation was confirmed with sequencing ...
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bioRxiv - Evolutionary Biology 2021Quote: Both HLS-C and HLS-E protein at 1 mg/mL were incubated with human Furin (EC 3.4.21.75, P09958, obtained from NEB P8077) in digestion buffer (10 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2022Quote: ... and transformed into competent DH5a E. coli (5-alpha Competent E. coli) following the protocol provided by the company (New England Biolabs, Ipswich, MA). For colony selection ...
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bioRxiv - Neuroscience 2020Quote: ... 0.35 μL DNA polymerase I (E. coli) (NEB, M0209), 0.09 μL DNA ligase (E ...
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bioRxiv - Immunology 2021Quote: ... E coli C2566 cells (New England Biolabs, Ipswich, MA) were transformed with the CuMVTT coat protein (CP ...
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bioRxiv - Molecular Biology 2024Quote: Escherichia coli (E. coli) strains such as DH5α (NEB), BL21 DE3 (NEB) ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... MBP-CENP-E was cloned into pMal-C2X (NEB). Bacterially-expressed constructs of GST-CENP-E were cloned in pET-3aTr (Tan ...
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Metabolomics reveals nucleoside analogs for regulating mucosal-associated invariant T cell responsesbioRxiv - Immunology 2023Quote: ... Escherichia coli (E. coli strain BL21, New England BioLabs) and Listeria monocytogenes (L ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli strain K12 (Turbo Competent E. coli, NEB #C2984) was used for cloning ...
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bioRxiv - Microbiology 2024Quote: ... coli Stable cells (NEB® Stable Competent E. coli) for amplification.
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bioRxiv - Cancer Biology 2024Quote: ... and transformation (chemically competent NEB 5-alpha E. coli) were performed according to manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: Library E was transformed into 5-alpha E.coli (NEB, C2987H), recovered in SOC medium (NEB ...
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bioRxiv - Microbiology 2022Quote: ... coli dam+/dcm+ (NEB 5-alpha competent E. coli, #C2987) and dam−/ dcm− (dam−/dcm− competent E ...
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bioRxiv - Immunology 2024Quote: ... The construct was transformed in T7 Express E coli (NEB) and grown to O.D ...
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bioRxiv - Synthetic Biology 2021Quote: ... Ni-NTA resin elute was immediately diluted with PBS containing 1 mM EDTA (PBS-E) and incubated with amylose resin (New England Biolabs) for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µL of linearised backbone and 1 µL digested pMB.BIG1a-e mix were used to set up a 20 µL Gibson Assembly (NEB #E2611) reaction ...
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bioRxiv - Biophysics 2023Quote: ... the mixtures were deproteinized by adding pronase E (4 μg/μl) and 1X purple gel loading dye (New England BioLabs) and incubated at 55°C for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: Escherichia coli (NEB® 10-beta Electrocompetent E. coli or NEB® 5-alpha Competent E ...
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bioRxiv - Microbiology 2024Quote: Quantification of SARS-CoV-2 E gene subgenomic mRNA (sgmRNA) was conducted using Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) on a Step One Plus Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli strain K12 (NEB Turbo Competent E. coli, New England Biolabs #C2984) was used for routine cloning ...
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bioRxiv - Biophysics 2024Quote: ... in Escherichia coli (E. coli) BL21(DE3) cells (New England Biolabs, MA) and protein purification was performed as described previously (61) ...
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bioRxiv - Microbiology 2021Quote: ... Yields of viral RNA were quantified by real-time qPCR by using SARS-CoV-2 specific primers targeting the E gene with the Luna®Universal One-Step RT-qPCR Kit (New England Biolabs) in a LightCycler 480 thermocycler (Roche ...
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bioRxiv - Bioengineering 2022Quote: Escherichia coli (E. coli) strain NEB 5-α (New England Biolabs, Hertfordshire, UK) was used for generation of genetic constructs ...
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bioRxiv - Physiology 2024Quote: ... or with live DH5α Escherichia coli (E. coli; New England Biolabs, catalog #C2987H). Doses are described in the text ...
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bioRxiv - Genomics 2024Quote: ... 5 units of PolyA polymerase (E. coli or yeast as appropriate) (NEB#M0276S). The reaction was incubated at 37°C for either 7 or 30 minutes and stopped by purification with a MEGAclear column (Termofisher AM1908 ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 μl of the assembly reaction was transformed into 5 μl chemically competent cells (E. coli NEB 5-alpha, New England Biolabs). Circuit constructs were analyzed by PCR ...
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bioRxiv - Bioengineering 2024Quote: ... containing 0.06 % pluronic acid (F-127, final concentration) and 1 µL of each disulfide bond enhancer 1 and 2 (NEB #E6820). We used droplet oil consisting of 3M™ Novec™ HFE7500 Engineered Fluid (3M ...
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bioRxiv - Microbiology 2022Quote: Escherichia coli DNA adenine methyltransferase (dam)+/dcm+ (NEB 5-alpha competent E. coli, #C2987) and dam−/dcm− (dam−/dcm− competent E ...
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bioRxiv - Evolutionary Biology 2024Quote: ... pBBR1-E and pBBR-EV were extracted using Monarch Plasmid Miniprep kit (#T1010; NEB) and following supplier’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Gibson assembly mix was transformed into competent cells (NEB, NEB Stable Competent E. coli), cells were spread on antibiotic resistant plates ...
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bioRxiv - Neuroscience 2024Quote: ... Gibson assembly mix was transformed into competent cells (NEB, NEB Stable Competent E. coli), cells were spread on antibiotic resistant plates ...
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bioRxiv - Biochemistry 2024Quote: ... the plasmid was transformed into commercially available Escherichia coli (E. coli) DH5α cells (NEB). The DNA was extracted following the QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µL ligation mix (2 µL 10x T4 ligase buffer, NEB, 1 µL T4 RNA ligase 2, truncated, NEB, 1 µL Ribolock inhibitor) was added and incubated (1 h ...
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bioRxiv - Developmental Biology 2024Quote: ... and the NEBNext Multiplex Oligos from Illumina (Index Primers Set 1, 2, 3 and 4, NEB, E7335S, E7500S, E7710S, E7730S), following the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2021Quote: ... C or E antibodies and the immunoprecipitates were treated with PNGase F (New England Biolabs) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... the pABA plasmid (from E. coli INV110) was methylated with Dam methylase (New England BioLabs), following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... The PCR fragment and pSLQ1651-sgTelomere (F+E) were digested with NheI and EcoRI (NEB), ligated and transformed into Stbl3 competent bacteria (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: Escherichia coli strain K12 (NEB Turbo Competent E. coli, New England Biolabs, catalog no. C2984) was used for routine cloning ...
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bioRxiv - Biochemistry 2021Quote: Xenopus laevis GINS was expressed in BL21 (DE3) Competent E coli Cells (C2527H, New England Biolabs) bacteria ...
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bioRxiv - Physiology 2024Quote: ... Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega. E. coli NEB 5α (NEB) was used as a host for plasmids ...
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bioRxiv - Physiology 2024Quote: ... Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega. E. coli NEB 5α (NEB) was used as a host for plasmids ...
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bioRxiv - Developmental Biology 2024Quote: ... et al.49 2mU6>sgRNA(F+E) plasmids were digested with BsaI-HF (NEB R3535, discontinued) in rCutsmart buffer for optimal positivity rates ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 1×Buffer 4 (NEB). The reaction was stopped (6 mM EGTA ...