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Citations for New England Biolabs :
651 - 700 of 1836 citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The lysate and the precipitates were treated with Lambda Protein Phosphatase (NEB) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... 1200 μg protein was mixed with 15 μL Glycoprotein Denaturing Buffer (NEB) in 150 μL total volume and incubated at room temperature for 10 min ...
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bioRxiv - Developmental Biology 2022Quote: ... while the other was treated overnight with Protein Deglycosylation Mix II (NEB) using non-denaturing reaction conditions according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... the MBP-fusion proteins were purified using amylose resin (New England Biolabs) and GST-fusions by glutathione-resin (GE Healthcare) ...
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bioRxiv - Plant Biology 2022Quote: ... incubated with λ protein phosphatase (40 units/μL) (P0753S, New England Biolabs) or recombinant TOPP4-GST/HIS for 1 h at 30□ ...
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bioRxiv - Plant Biology 2022Quote: ... MBP-fusion proteins were purified using Amylose Resin (New England Biolabs, E8021), following the manufacturer’s instruction with minor modification ...
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bioRxiv - Microbiology 2023Quote: ... and then associated with EnGen Spy Cas9 NLS protein (New England BioLabs) using the 4D-Nucleofector system (Lonza) ...
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bioRxiv - Cell Biology 2023Quote: ... and MBP-tagged proteins were purified on Amylose resin (New England Biolabs).
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins were purified on glutathione-Sepharose beads (New England Biolabs), and MBP-tagged proteins were purified on Amylose resin (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, E6800L) was used as described previously (31) ...
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bioRxiv - Cell Biology 2023Quote: ... 25 µl protein A magnetic beads S1425S (New England Biolabs; Ipswich, MA) were washed 3 times in PBS and incubated overnight with 2-3 µg of antibody directed against CISD34 at 4°C with gentle mix ...
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bioRxiv - Developmental Biology 2023Quote: ... sgRNA was incubated at room temperature with Cas9 protein (NEB, Cat# M0646T). AB* embryos were injected at the one-cell stage with the sgRNA and Cas9 cocktail in a 1nL volume at 25pg sgRNA/nL ...
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were lysed and subsequently incubated with λ-protein phosphatase (NEB) for 1 h at 30°C according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... The proteins were purified by NEBExpress Ni Spin Columns (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... 2.5 μL 10 mM MnCl2 and 2 μL Lambda Protein Phosphatase (NEB). The dephosphorylation reaction was allowed to occur at 30 °C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins with MBP-tag were loaded onto amylose resin (New England Biolabs) after elution from Ni-NTA ...
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bioRxiv - Neuroscience 2023Quote: ... This complex was co-injected with Cas9 protein (M0386M, NEB, Ipswich USA) into the yolk of 1-cell stage WT zebrafish embryos ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL of 2X protein synthesis buffer (New England Biolabs, Beverly, MA), 0.25 μL of RNaseOUT RNAse inhibitor (Thermo-Fisher ...
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Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2023Quote: ... The diluted sample was treated with 2 µl Lambda protein phosphatase (NEB) and incubated in water bath at 30 °C for 4 hrs ...
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bioRxiv - Molecular Biology 2024Quote: ... including a Blue Pre-stained Broad Range Protein Standard (New England Biolabs). Gels were either stained (with 0.5% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... The molecular weight standards used were unstained broad range protein standards (NEB) and then the gel was run at 100 volts until the dye front was 1 cm from the bottom of the gel and then stained one hour in Coomassie blue stain (50% methanol ...
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bioRxiv - Cell Biology 2020Quote: ... samples were cooled and then digested with DpnII for one hour at 37° C (NEB, R0543). These ligated pools were then amplified using AdR_PCR oligonucleotides as primer (5′-GGTCGCGGCCGAGGATC-3′ ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants were thawed at 37°C and mixed with 20 µL of a 500U DNaseI (NEB), 10X buffer and nuclease free water mixture and incubated at 37°C for 30 minutes ...
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bioRxiv - Biophysics 2021Quote: ... 72 °C for 5 minutes) using Phusion High-Fidelity PCR Master Mix with HF buffer (NEB), 0.1 µM of PE1.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... were incubated for 12 hours at 30°C with 7.5 Units of Phi29 polymerase (NEB M0269) in Phi29 NEB buffer 1X ...
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bioRxiv - Systems Biology 2019Quote: ... 30°C and the growth was continuously monitored online using Cell Growth Quantifier (Aquila Biolabs GmBH). Samples were withdrawn at regular intervals for analyzing the spent metabolites through High-Performance Liquid Chromatography (HPLC).
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bioRxiv - Microbiology 2019Quote: ... which was placed towards the centre of a C760 Innova – 70 °C freezer (New England Biolabs) for 1 h before being thawed in a 37 °C waterbath for approximately two min.
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bioRxiv - Molecular Biology 2020Quote: ... After incubation for min at 55°C 1 μl T4 ligase (400,000 units/ml, NEB, M0202) was added ...
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bioRxiv - Genetics 2019Quote: The product was digested overnight at 37°C using 20 U PmlI (New England Biolabs, USA), 5 uL accompanying buffer ...
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bioRxiv - Microbiology 2019Quote: ... and 4 μL was ligated at 16°C overnight with the T4 DNA ligase (NEB M0202S). After transformation into chemically-competent Top10 E ...
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bioRxiv - Cell Biology 2020Quote: ... pH 8.6) overnight at 37 °C in the presence of non-tritiated SAM (New England Biolabs). Samples were resolved via SDS-PAGE ...
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bioRxiv - Genomics 2019Quote: ... Tagmented DNA was End-Repaired for 30 minutes at 37°C (5U Klenow Exo-(NEB #M2012S), 1 × NEB Buffer 2 ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were stored at -20° C before PCR amplification of cDNA with Q5 (New England Biolabs) or SuperFiII (ThermoFischer).
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bioRxiv - Molecular Biology 2022Quote: ... and were ligated over night at 16 °C using T4 DNA ligase (New England Biolabs, UK). The ligation mix was transformed into E ...
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bioRxiv - Microbiology 2021Quote: ... after ligation overnight at 4°C with the T4 DNA ligase (New England Biolabs, Evry, France).The sequences of the cloned fragments in the resulting plasmids pYES2::MlpCSP1 ...
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bioRxiv - Microbiology 2021Quote: ... immediately rescued in 1 ml of 37°C SOC outgrowth medium (New England Biolabs, cat#B9020S) and incubated with shaking at 37°C for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... PCR products were incubated overnight at 60 °C with either BstNI restriction enzyme (New England Biolabs) or a control buffer solution without enzyme ...
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bioRxiv - Molecular Biology 2022Quote: ... and were ligated over night at 16 °C using T4 DNA ligase (New England Biolabs, UK). The ligation mix was transformed into E ...
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bioRxiv - Microbiology 2022Quote: ... Samples were treated twice for 45 minutes at 37°C with DNase I (New England Biolabs) with heat inactivation at 75°C for 10 minutes and DNA was further removed using a RNeasy Mini kit (Qiagen) ...
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bioRxiv - Microbiology 2019Quote: ... Individual fragments were amplified at 60°C with Phusion® High-Fidelity Polymerase (New England Biolabs) using T ...
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bioRxiv - Physiology 2021Quote: ... The pNB1u vector was digested overnight at 37 °C with PacI and Nt.BbvCI (New England Biolabs) to generate 8-nt overhangs ...
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bioRxiv - Biochemistry 2019Quote: ... 4°C) and the supernatant was added to 24 mL of amylose resin (NEB, Ipswich MA) pre-equilibrated with buffer C and nutated for 2 hr at 4°C ...
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bioRxiv - Genomics 2021Quote: ... followed by an overnight incubation at 16°C with 3 µL T4 DNA ligase (NEB, M0202). Samples were purified with phenol-chloroform and used as 3C templates for Taqman-qPCR ...
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bioRxiv - Biochemistry 2020Quote: ... Reaction mixtures were treated with proteinase K at 37 °C for 10 min (New England Biolabs) and the RNA samples were purified by RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Genetics 2021Quote: ... for 1 hour at 37°C into pPB_U6::gRNA_EF1a::BFP-Puro digested with BlpI (NEB #R0585S) and BstXI (NEB #R0113S) ...
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bioRxiv - Genomics 2019Quote: ... samples were cooled and then digested with DpnII for one hour at 37°C (NEB, R0543). These ligated pools were then amplified using AdR_PCR oligonucleotides as primer (5′ -GGTCGCGGCCGAGGATC-3′ ...
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bioRxiv - Neuroscience 2020Quote: ... After overnight incubation at 4°C in PBS-Perm with rabbit anti-GFP (1:1000, Biolabs) and mouse anti-MAP2 (1:2000 ...
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bioRxiv - Cell Biology 2020Quote: ... pFA6a-mto2S338N-C-KanMX6 was generated using NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs) in an assembly containing 5 fmol of each fragment ...
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bioRxiv - Biochemistry 2022Quote: In vitro coupled transcription-translation was performed at 37 °C using the PURExpress ΔIleRS Kit (NEB) following manufacturer’s protocol supplemented with [35S]-methionine (EasyTag ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid and insert were then ligated overnight at 16 °C (T4 DNA ligase, New England Biolabs). After transformation into chemically competent E.coli and subsequent plasmid purification ...