Labshake search
Citations for New England Biolabs :
401 - 450 of 8749 citations for Butyric Acid BA CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... NEBuilder HiFi DNA Assembly Cloning Kit (NEB #E5520) was used to introduce upstream and downstream homology arms (1000bp each ...
-
bioRxiv - Genetics 2023Quote: ... with Phusion high-fidelity DNA Polymerase Kit (NEB), Common sgRNA-R and Primer 1 or Primer 2 (see below).
-
bioRxiv - Plant Biology 2023Quote: ... with Gibson Assembly cloning kit (NEB, Evry, France). Plasmids were introduced into NEB 5α competent E ...
-
bioRxiv - Microbiology 2023Quote: ... were annealed with the Quick Ligation Kit (NEB). The DNA was then digested with PacI (NEB ...
-
bioRxiv - Microbiology 2023Quote: ... The Monarch PCR and DNA Cleanup Kit (NEB) and Monarch DNA Gel Extraction Kit (NEB ...
-
Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... assembly with the NEBuilder HiFi kit (NEB #E2621S), and heat-shock transformation into competent E ...
-
bioRxiv - Microbiology 2023Quote: ... using the NEB Next RNA Library kit (NEB) with indexing ...
-
bioRxiv - Cancer Biology 2023Quote: ... Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
-
bioRxiv - Molecular Biology 2023Quote: ... HiScribe T7 High Yield RNA Synthesis Kit (NEB) was used for in vitro transcription ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Recovered fragments were assembled using NEBuilder kit (NEB). Assembled plasmids were sequenced by the Medical Research Council Protein Phosphorylation and Ubiquitylation Unit’s DNA sequencing service at the University of Dundee.
-
bioRxiv - Molecular Biology 2023Quote: ... with NEBNext rRNA Depletion Kit V2 (NEB E7400). RNA-seq samples were prepared with single index primers (NEB ME6609S) ...
-
bioRxiv - Cancer Biology 2023Quote: ... cloned using the TOPO Blunt cloning kit (NEB), and sent for Sanger sequencing ...
-
bioRxiv - Molecular Biology 2023Quote: ... combined with the NEBNext rRNA Depletion kit (NEB). Complementary DNA quality and concentrations were assessed using the High Sensitivity dsDNA kit (Agilent ...
-
bioRxiv - Synthetic Biology 2023Quote: ... miniprepped (Monarch Plasmid Miniprep Kit, New England Biolabs), and transformed into E ...
-
bioRxiv - Genetics 2023Quote: ... using the Gibson Assembly Cloning Kit (NEB, E2611S). pGADT7-Rnf212bC9A ...
-
bioRxiv - Microbiology 2023Quote: ... the Q5® Site-Directed Mutagenesis Kit (NEB) was used with primer pairs designed using the manufacturer’s online software (nebasechanger.neb.com) ...
-
bioRxiv - Immunology 2023Quote: ... using an NEBuilder HiFi DNA Assembly kit (NEB). The gmlABC genes used to make serotype O2v2 were PCR-amplified from NTUH gDNA using primers gmlABC_1F/gmlABC_1R and assembled with XbaI restriction enzyme-linearized pACT3 vector ...
-
bioRxiv - Microbiology 2023Quote: ... The Q5 NEB Mutagenesis Kit (New England Biolabs) was used to add an NES (LQKKLEELEL ...
-
bioRxiv - Genetics 2023Quote: ... using the Q5 site-directed mutagenesis kit (NEB). mScarlet-I was paired with a HygR marker (Dickinson et al. ...
-
bioRxiv - Genetics 2024Quote: ... with Phusion high-fidelity DNA Polymerase Kit (NEB), Common sgRNA-R and Primer 1 or Primer 2 (see below).
-
bioRxiv - Developmental Biology 2023Quote: ... site-directed mutagenesis (Q5 Kit, New England Biolabs) of pCS2(+)-vg1-sfGFP was performed to truncate the full-length construct into respective domains (as depicted in Figure 3) ...
-
bioRxiv - Plant Biology 2024Quote: ... or the Q5 Site Directed mutagenesis Kit (NEB) according to the supplieŕs instructions were used.
-
bioRxiv - Synthetic Biology 2024Quote: ... The Quick Ligation™ Kit (New England Biolabs) was used to ligate the vector and backbone fragments ...
-
bioRxiv - Microbiology 2023Quote: ... using the Quick Ligase kit (New England Biolabs), and the ligation mix was used to transform the Escherichia coli M15 strain ...
-
bioRxiv - Molecular Biology 2023Quote: ... using the Q5-site directed mutagenesis kit (NEB). The 3×HA epitope tag was amplified from the pPR2-HA3 plasmid (Katris et al ...
-
bioRxiv - Biochemistry 2024Quote: A Q5 Site-Directed Mutagenesis Kit (NEB, E0554S) was used to introduce mutations in amino acid residues predicted to play a role in stabilizing zinc ions ...
-
bioRxiv - Cancer Biology 2024Quote: ... and purification with Monarch RNA Cleanup Kit (NEB). RNAs were then used for MeRIP assays.
-
bioRxiv - Bioengineering 2024Quote: ... and a Monarch PCR & DNA Cleanup kit (NEB) with the following changes ...
-
bioRxiv - Biophysics 2024Quote: ... Gibson Assembly Cloning Kit (NEB, Ipswich, MA, USA) and 5-alpha E ...
-
bioRxiv - Molecular Biology 2019Quote: ... 1 µg of purified total RNA’s was used for rRNA depletion using Ribo-Zero Gold rRNA Removal Kit (Illumnia, MRZG 12324) or NEBNext rRNA Depletion Kit (NEB, E6310). Library preparation was done according to the manufacturer’s instructions with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The library of each sample was prepared separately with the NEBNext® Ultra (tm) II FS DNA Library Preparation Kit and the Unique Dual Indexing kit (New England Biolabs), with reagent volumes reduced to one-fifteenth of that advised by the manufacturer ...
-
bioRxiv - Neuroscience 2022Quote: ... was then gel purified using the Qiagen MinElute Gel Extraction Kit (28604) and subjected to Gibson assembly via the New England Biosciences HiFi DNA Assembly Cloning Kit (NEB: E5520S) to insert the amplified region into the hSyn-eGFP vector following its linearization with BamHI (NEB ...
-
bioRxiv - Molecular Biology 2020Quote: ... Libraries were qualified on Agilent 2100 Bioanalyzer using High Sensitivity DNA kit and quantified by qPCR using NEBNext Library Quant kit for Illumina (NEB, E7630). Equal molarity of each library were pooled and subjected to sequencing on Illumina HiSeq 4000 and NovaSeq 6000 platforms at the McGill University and Génome Québec Innovation Centre to generate 50 bp paired-end reads.
-
bioRxiv - Pathology 2021Quote: ... The kit used for library construction was the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA). Total RNA extracted from the samples was subject to mRNA enrichment using poly-T oligo-attached magnetic beads ...
-
bioRxiv - Genomics 2021Quote: ... We individually indexed samples to be pooled within the same sequencing lane during the PCR enrichment step using NEBNext® Multiplex Oligos for Illumina® (single-indexed with E7335 and E7500 kits, or dual-indexed with E6440 kit, New England Biolabs). We performed in-solution hybridization and target enrichment using our 12,049 tiled RNA probes ...
-
bioRxiv - Genetics 2021Quote: ... Vector components were purified using the Monarch® DNA Gel Extraction Kit or the Monarch® PCR & DNA Cleanup Kit (NEB) and assemblies were performed using NEBuilder® HiFi DNA Assembly Master Mix (NEB) ...
-
bioRxiv - Molecular Biology 2022Quote: RNA-seq libraries for RIP-TRIBE and polysome were prepared in triplicates from 150 ng of total RNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with the NEBnext rRNA depletion Kit (NEB #E6310L). cDNA libraries were sequenced on an Illumina NovaSeq6000 using S-prime or S1 flow cells with paired-end 150-bp reads ...
-
bioRxiv - Biochemistry 2022Quote: ... RNA-seq libraries were generated using the NEBNext Globin&rRNA depletion kit and the NEBNext UltraII Directional RNA Library prep kit according to the manufacturer’s instructions (New England Biolabs, MA, USA). Sequencing experiments were performed with NextSeq 500 (Illumina ...
-
bioRxiv - Cancer Biology 2022Quote: ... RNA-seq libraries were generated using the NEBNext Globin&rRNA depletion kit and the NEBNext UltraII Directional RNA Library prep kit according to the manufacturer’s instructions (New England Biolabs, MA, USA). Sequencing experiments were performed using NextSeq 500 (Illumina ...
-
bioRxiv - Genomics 2022Quote: We constructed NEBNext libraries with 200- and 10-ng RNA by following the guideline of NEBNext Ultra II RNA Library Prep Kit for Illumina kit (NEB, E7770). SmartSeq2 libraries with single cells were prepared following the protocol that was reported by Picelli ...
-
bioRxiv - Molecular Biology 2022Quote: ... Library quality was checked by Agilent BioAnalyzer 2100 using the High Sensitivity DNA kit (Part number 5067-4626) and libraries were quantified using the Library Quant Kit for Illumina (NEB #7630). Libraries were then sequenced using a NextSeq500 platform (75-base-pair (bp ...
-
bioRxiv - Molecular Biology 2022Quote: ... Library quality was checked by Agilent BioAnalyzer 2100 using the DNA 1000 kit (Part number 5067-1504) and libraries were quantified using the Library Quant Kit for Illumina (NEB #7630). Libraries were then sequenced using a NextSeq500 platform (75-base-pair (bp ...
-
bioRxiv - Biochemistry 2023Quote: ... loci were amplified using the NGSgo®-MX6-1 kit (GenDx) and multiplexed sequencing libraries were prepared using the Ultra™ II FS DNA Library Prep Kit for Illumina (NEB). Libraries were sequenced on a NovaSeq 6000 system (Illumina ...
-
bioRxiv - Immunology 2023Quote: ... These amplicons were used as templates for in vitro transcription using the HiScribe T7 mRNA Kit with CleanCap® Reagent AG kit (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Reverse transcription into cDNA was performed using a cDNA synthesis kit (ProtoScript First Strand cDNA Synthesis Kit, New England BioLabs, Ipswich) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were generated using the NEBNext Globin&rRNA depletion kit and the NEBNext UltraII Directional RNA Library prep kit according to the manufacturer’s protocols (New England Biolabs, MA, USA), as described previously58 ...
-
bioRxiv - Molecular Biology 2023Quote: Libraries were qualified on Agilent 2100 Bioanalyzer using High Sensitivity DNA Kit and quantified by qPCR using NEBNext Library Quant Kit for Illumina (NEB, E7630). Equal molarity of each library was pooled and subjected to sequencing on Illumina NovaSeq 6000 platform at the McGill University and Génome Québec Innovation Centre to generate 100 bp paired-end reads.
-
bioRxiv - Biophysics 2023Quote: ... Vector DNA was linearized with NheI or MluI and cRNA synthesized in vitro using the SP6 or T7 AmpliCap Max High Yield Message Maker Kit (Cellscript, USA) or HiScribe® T7 ARCA mRNA Kit (New England Biolabs) and stored at −20°C (for frequent use ...
-
bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized with RevertAid First Strand cDNA Synthesis Kit (ThermoScientific) reverse transcription kit and PCR was carried out using OneTaq® DNA Polymerase reagents (NEB) using conditions previously detailed and oligonucleotides described in Suppl ...
-
bioRxiv - Cell Biology 2024Quote: ... samples were run on a 1% agarose gel and areas corresponding to the expected size were excised and purified from the gel using a gel extraction kit (Monarch DNA Gel Extraction Kit, NEB, USA). This PCR product was used for a second round of amplification followed by gel purification ...