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Citations for New England Biolabs :
1 - 50 of 653 citations for Brucella Blood Agar with Hemin and Vitamin K 15x100mm plate 26ml fill since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Subsequent steps (end fill-in with T4 DNA polymerase (NEB), ligation to biotinylated adaptors ...
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bioRxiv - Molecular Biology 2022Quote: ... gap fill enzymes were added (1U Sulfolobus DNA Polymerase IV NEB cat# M0327S and 10U E ...
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bioRxiv - Genetics 2020Quote: ... followed by fill-in with T4 DNA polymerase (New England Biolabs, Beverly, MA, USA), by adding to the annealing mix 2.5 µl 10 mM dNTPs ...
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bioRxiv - Microbiology 2024Quote: ... Colony PCR was performed on Salmonella-positive colonies on blood agar using Taq DNA polymerase (New England Biolabs®) with an annealing temperature of 55°C and an extension time of 2.5 min ...
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bioRxiv - Microbiology 2024Quote: ... coli DH5α F’Iq cells and transformants were selected on LB agar plates containing carbenicillin (NEB). The DNA sequence of the plasmid inserts for each transformant was verified before and after transformation into STm by electroporation ...
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bioRxiv - Genomics 2020Quote: ... followed by a fill-in reaction with adapter sequences (Bst DNA polymerase, New England Biolabs; 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a fill-in reaction using DNA polymerase I large (Klenow) fragment (New England Biolabs), and re-ligation with T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Biochemistry 2022Quote: ... The annealed product was labeled by fill-in synthesis using 5 U Klenow exo-(New England Biolabs) and a dNTP mix containing 40 µM each of dATP ...
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bioRxiv - Cancer Biology 2021Quote: ... proteinase K (NEB) and heat for decrosslinking followed by Ampure XP beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... Proteinase K (NEB) (digests most peptide bonds ...
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bioRxiv - Immunology 2020Quote: ... Proteinase K (NEB) for 1 hour at 50°C ...
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bioRxiv - Microbiology 2023Quote: ... Proteinase K (NEB) and incubated at 56 degrees C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... proteinase K(NEB) and RNase A (Solarbio ...
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bioRxiv - Microbiology 2023Quote: ... proteinase K (NEB) (10 μl of 20mg/mL ...
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bioRxiv - Genomics 2023Quote: ... proteinase K (NEB) for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... The fill-in reaction consisted of a 25 μl Phusion High-Fidelity Master Mix 2X (New England Biolabs), 23 μl of water and 1ul of each oligo at a concentration of 10 μM ...
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bioRxiv - Genetics 2024Quote: ... KAPA HiFi Uracil+ DNA polymerase (KapaBiosystems) was used to fill in the gaps and Taq DNA ligase (NEB) was applied to seal the nicks ...
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bioRxiv - Genomics 2023Quote: ... Transposed DNA was gap-filled by addition of 4μL gap-fill mix (3.2 μL Q5 reaction buffer (NEB), 0.07 μL 1M MgCl2 ...
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bioRxiv - Genomics 2023Quote: ... Gaps in the transposed DNA were filled by 15μL gap-fill mix (7 μL Q5 reaction buffer (NEB), 7 μL Q5 high GC enhancer (NEB) ...
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bioRxiv - Genomics 2020Quote: ... is added to the Proteinase K (NEB Proteinase K #P8107S; ProK) reactions to inactive the ProK prior to coupling to streptavidin beads ...
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bioRxiv - Genomics 2021Quote: DNA ends were marked with biotin-14-dATP by adding 60μL of biotin fill-in master mix (1X NEB 3.1 ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was labeled by fill-in of EcoRI overhangs with the DNA polymerase I Klenow fragment (New England Biolabs) in the presence of [α-32P] dATP (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2022Quote: ... and proteinase K (NEB). The substrate was obtained by PCR on CML cells derived gDNA using Q5 DNA polymerase (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... Protease K (P8107S, NEB). Antibodies used were ...
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bioRxiv - Microbiology 2023Quote: ... and Proteinase K (NEB). Genomic DNA was sonicated (Q800R2 QSONICA ...
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bioRxiv - Genomics 2023Quote: ... and proteinase K (BioLabs) treatment ...
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bioRxiv - Genetics 2024Quote: ... the proteinase K (NEB) was added and incubated for 2 h at 55 °C to digest protein and the RNase A (Thermo ...
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bioRxiv - Plant Biology 2024Quote: ... Proteinase K (NEB, P8107S) and RNase A (20 mg/ml ...
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bioRxiv - Biochemistry 2024Quote: ... and proteinase K (NEB), followed by phenol–chloroform extraction and ethanol precipitation ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteinase K (NEB P8107S) incubation was also performed overnight at 65 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... sufficient colonies were scraped from agar plates for >400-fold library coverage and DNA was extracted (T1010L, New England Biolabs). DNA concentrations were measured using a NanoPhotometer N60 (Implen).
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bioRxiv - Biochemistry 2020Quote: ... The substrate was designed such that the digested strand is 6 nt shorter than the undigested strand to allow 3′ fill-in by Klenow Fragment (3′-5′ exo-; NEB) with α[32P]-dCTP and cold dATP ...
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bioRxiv - Molecular Biology 2021Quote: ... the samples were treated with Klenow exo- at 37 °C for 30 min to fill the ends of adaptors and then trimmed with T4 DNA polymerase (NEB) at 12 °C for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Biotin fill in was performed by addition of 52uL fill-in mix (0.288mM dCTP/dTTP/dGTP, 50U DNA Polymerase I, Large Klenow fragment (NEB M0210) and 0.288mM biotin-dATP (Thermo 19524016)) ...
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bioRxiv - Molecular Biology 2020Quote: ... the ligated DNA was subjected to a Fill-in reaction by resuspending the beads in 40 μL of 1X phi29 buffer (NEB) containing 0.2 mg/mL BSA (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... Klenow was then used to fill in restriction fragment overhangs and mark the DNA ends with biotin (M0210, New England Biolabs). Proximity ligation contact (PLC ...
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bioRxiv - Systems Biology 2020Quote: ... The full volume of each fill-in reaction was put into a dilute blunt-end ligation reaction (640 U T4 DNA Ligase, NEB) and allowed to incubate overnight at 15° C ...
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bioRxiv - Bioengineering 2022Quote: ... a gene-specific oligonucleotide was annealed to a constant oligonucleotide encoding the CRISPR-Cas9 scaffold and then a fill-in reaction was performed using T4 DNA polymerase (New England Biolabs). For the tyr_1 and tyr_2 sites ...
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bioRxiv - Immunology 2023Quote: ... were incorporated onto the mab-oligos via a 50 μl gap-fill ligation reaction consisting of 40 U Taq ligase (New England Biolabds) 3 U T4 DNA polymerase (New England Biolabs), 100 μM dNTPs ...
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bioRxiv - Biophysics 2021Quote: Polymerised gels were removed from the coverslip chamber and placed into 6-well plates to undergo digestion in 0.2 mg/ml proteinase K (New England Biolabs) dissolved in digestion buffer (50 mM Tris pH 8.0 (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was discarded and the nuclei were resuspended in 300 μl Fill-in Buffer containing 30 μl Klenow Buffer 10X (NEB, M0210L), 15 μl1 mM Biotin-16-dCTP (Jena Bioscience ...
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bioRxiv - Neuroscience 2021Quote: Proteinase K (New England Biolabs) was diluted 1:100 to 8 units/mL in digestion buffer (50 mM Tris (pH 8) ...
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bioRxiv - Genomics 2020Quote: ... Proteinase K (New England Biolabs) was added to a final concentration of 4 units/ml and incubated 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... and 0.8U Proteinase K (NEB) for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Proteinase K (New England BioLabs) and dithiothreitol (DTT).
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bioRxiv - Biochemistry 2022Quote: ... Thermolabile Proteinase K (NEB, P8111S) with 15 mM EDTA was added to the reaction ...
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bioRxiv - Microbiology 2020Quote: ... 12.5 μl Proteinase K (NEB), and 24 μl H2O and incubated samples for 1.5 hours at 55°C ...
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bioRxiv - Immunology 2020Quote: ... supplemented with Proteinase K (NEB), and incubated 1?h at 56°C ...
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bioRxiv - Microbiology 2020Quote: ... 2 μl Proteinase K (NEB) was added and samples were incubated at 56°C for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... 1% Proteinase K (NEB, P8107S) solution ...