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Citations for New England Biolabs :
101 - 150 of 4058 citations for Breast Cancer Type 1 Susceptibility Protein BRCA1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: 1 μg of N protein was dissolved in 36 μL H218O and 2 μL 10x glycobuffer (NEB). To this solution 2 μL PNGase F was added and the reaction mixture was incubated at 37 °C for 16 h ...
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bioRxiv - Biochemistry 2021Quote: ... with and without the addition of ACE2 (~1:1.25 S-protein trimer:ACE2 molar ratio) (New England Biolabs). Vitrified samples of S-protein constructs with and without ACE2 were prepared by first glow discharging Quantifoil R1.2/1.3 300 mesh holey carbon copper grids for 1 minute using a Pelco easiGlow glow discharge unit (Ted Pella ...
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bioRxiv - Biochemistry 2023Quote: ... Bound protein was detected by western blot using mouse anti-MBP diluted 1:10,000 (New England Biolabs), fluorescent secondary antibodies (Li-Cor Biosciences) ...
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bioRxiv - Cell Biology 2023Quote: ... 1-cell stage embryos were injected with two guides for each gene with Cas9 protein (NEB, M0646T). Tyrosinase was an injection control to validate the efficacy of the Cas9 protein ...
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bioRxiv - Genomics 2021Quote: ... gDNA was digested with the CspCI Type IIB restriction enzyme (IIB-REase - New England BioLabs, Inc.) which has shown to yield a high marker density in triatomine65 ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by digestion of the wild-type strand with the enzyme BsurI (New England Biolabs, R0581S)[27] ...
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bioRxiv - Microbiology 2020Quote: ... The wild-type Chlamydia trachomatis ct276 gene was amplified by PCR with Phusion DNA polymerase (NEB) using 100 ng C ...
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bioRxiv - Synthetic Biology 2023Quote: ... Golden gate (Type-IIS) cloning was conducted using chemically competent Escherichia coli DH5α cells (NEB, C2987H). Construction of destination vectors with the toxic selection marker ccdB was done with chemically competent One Shot ccdB Survival 2 T1R E ...
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bioRxiv - Cancer Biology 2021Quote: ... we used 50 μL Protein A or Protein G Magnetic beads (NEB) and washed twice with PBS with 5 mg/ml BSA and 4 μg of antibody coupled in 500 μl PBS with 5 mg/ml BSA overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant Cas9 protein (NEB) and sgRNA prepared by in vitro transcription were added to the mixture and incubated at 37℃ for ∼ 4h to remove rRNA ...
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bioRxiv - Neuroscience 2021Quote: ... Lambda Protein Phosphatase (NEB) assay was performed as described by the supplier ...
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bioRxiv - Biochemistry 2020Quote: ... Cas9 protein (NEB M0641S) and phenol red injection dye ...
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bioRxiv - Developmental Biology 2021Quote: Cas9 protein (M0646, NEB) and sgRNAs against antisense of exons 14 ...
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bioRxiv - Neuroscience 2020Quote: ... + 5 % non-fat milk for 1 hour at room temperature before incubating with primary antibody: anti-GFP (1:1000, Biolabs) or anti-actin (1:5000 ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed three times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (anti-rabbit IgG HRP, 1:5000 dilution, 7074S, NEB) in TBS-T with 1% (w/v ...
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bioRxiv - Plant Biology 2021Quote: Purified LPR1 protein was analyzed using Protein Deglycosylation Mix II (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Protein (200 µg) was precleared with protein G magnetic beads (New England Biolabs) and incubated overnight (4° C ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleaved G protein was dephosphorylated by lambda protein phosphatase (NEB, Frankfurt, Germany), calf intestinal phosphatase (NEB ...
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bioRxiv - Microbiology 2023Quote: ... The protein ladder used was Colour Protein Standard Broad Range (NEB, Ipswich, US).
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bioRxiv - Cancer Biology 2020Quote: Specific antibodies against Slug (C19G7, Cell Signaling Technology, NEB, Frankfurt, Germany, #9585, 1:400), pan-cytokeratine (polyclonal ...
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Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... Cells were then incubated for 1 hour with anti-MBP antibody (New England BioLabs) solution diluted in calcium containing blocking buffer ...
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bioRxiv - Evolutionary Biology 2021Quote: Both HLS-C and HLS-E protein at 1 mg/mL were incubated with human Furin (EC 3.4.21.75, P09958, obtained from NEB P8077) in digestion buffer (10 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... Cas9 RNPs were formed by incubating 1 pmol of sgRNA with 0.5 pmol of Cas9 protein in 1X NEBuffer™ 3.1 or 2.1 (NEB) at room temperature for 10 min ...
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bioRxiv - Bioengineering 2023Quote: ... 0.27 nmol ErCas12a/LbCas12aU protein and 0.45 nmol crRNA were assembled in 1 X Nuclease Reaction Buffer (NEB). The protein and RNA were mixed and incubated for 10 minutes at room temperature and used for transformation of embryogenic C ...
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bioRxiv - Molecular Biology 2021Quote: ... Mutations were introduced into UNC-45B wild-type using the Q5® Site-Directed Mutagenesis Kit (NEB). Recombinant protein expression was induced in BL21 DE3 when optical density (OD600 ...
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bioRxiv - Microbiology 2019Quote: ... Genes of interest were amplified from Synechocystis 6803 wild type genomic DNA with the Phusion Polymerase (NEB) according to manufacturer’s guidelines ...
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bioRxiv - Plant Biology 2019Quote: ... the sgRNA vectors were digested with the type IIS restriction enzyme BsaI (New England Biolabs, cat#R0535S) and dephosphorylated with Calf Intestinal Alkaline Phosphatase (Takara ...
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bioRxiv - Microbiology 2019Quote: ... The resulting plasmid pFN_7_37_2k_kanRn was generated by the type IIS restriction enzyme BbsI and T4 DNA ligase (both NEB, USA). Another version of kanR with slightly different 5’ and 3’UTR was generated by PCR using the primer pair F13 ...
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bioRxiv - Cell Biology 2022Quote: ... the wild-type and OGG1 (G245A) coding sequences were amplified by PCR with Phusion DNA polymerase (NEB) (primers indicated in Table S2 ...
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bioRxiv - Immunology 2023Quote: ... we pooled all the eBlocks at equal molar ratio and used PaqCI Type IIs restriction enzyme (NEB), NEBridge Ligase Master Mix (NEB) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The variants were created from the wild type plasmids using the Q5 Site-Directed Mutagenesis Kit (NEB) and transformed into MegaX cells made chemically competent (Mix & Go E ...
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bioRxiv - Developmental Biology 2023Quote: ... and ligated into the BioID plasmid using type II restriction enzymes BamHI and XhoI (New England BioLabs) and T4 DNA ligase ...
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bioRxiv - Biochemistry 2024Quote: The cloning of human wild type Mad2 and the mutant Mad2R133A by the USER® method (NEB) into the pRSFDuet-1 vector (71341-3 ...
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bioRxiv - Cell Biology 2024Quote: ... wild-type or mutant VPS35L sequences were amplified using Q5 High-Fidelity 2X Master Mix (NEB, M0492) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Protein molecular ladder (Color Prestained Protein Standard, Broad Range 11–245 kDa, NEB, P7712S). Dry transfer was performed using an iBlot™ 2 gel transfer device (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... purified proteins were directly digested by O-glycosidase (P0733, NEB, 4,000 units/µg protein) and α2-3 ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extracts were also treated with Lambda Protein Phosphatase (Lambda PP, New England BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: Protein expression was performed with the PURExpress In Vitro Protein Synthesis kit (E6800, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... 15µl to 25µl of extracted proteins in NuPAGE buffer and 0.5µl protein ladder (BioLabs) were loaded into a Bio-Rad 4-15% Mini-PROTEAN precast polyacrylamide gel ...
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bioRxiv - Molecular Biology 2021Quote: ... Lambda protein phosphatase (λPP) (NEB) was used to dephosphorylate the purified chromatin ...
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bioRxiv - Developmental Biology 2021Quote: ... 1.9nM Cas9 protein (NEB M0386T) and 14% Phenol Red ...
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bioRxiv - Genetics 2019Quote: ... Cas9 protein (20 µM, NEB) was mixed in a 1:1 ratio with the gRNA complex and incubated for 5-10 minutes at room temperate to produce the Cas9:gRNA RNP complex at a final concentration of 10 µM ...
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bioRxiv - Biochemistry 2022Quote: ... Lambda protein phosphatase (P0753, NEB), AZD8055 (S1555 ...
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bioRxiv - Immunology 2022Quote: ... Protein G magnetic beads (NEB) were incubated with anti-2A (Novus ...
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bioRxiv - Developmental Biology 2019Quote: ... and Cas9 protein (NEB M0386T) into the yolk of 1-cell stage embryos in a volume of 1nl ...
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bioRxiv - Developmental Biology 2021Quote: ... or Cas9 protein (NEB, M0646), and 200 ng/ul of guide RNA ...
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bioRxiv - Molecular Biology 2022Quote: ... with Protein Kinase K (NEB) and incubated at 37 °C for 1 h ...
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bioRxiv - Physiology 2023Quote: ... Cas9 protein (New England Biolabs) and PAGE purified ssHDR template (Integrated DNA Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... Cas9 protein (NEB, Ipswich, MA), and ssODN (Integrated DNA Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... coli Protein Synthesis System (NEB) [48] at 2 x the reaction scale ...