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Citations for New England Biolabs :
1 - 50 of 109 citations for Borrelia burgdorferi sensu stricto B31 Surface Lipoprotein P27 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... burgdorferi cells (62) using QuickLoad 2x Taq Master Mix (NEB, M0271) and primers 49 and 50 (Table 1) ...
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bioRxiv - Microbiology 2022Quote: The full-length badR gene was amplified from the genome DNA of the wild-type Borrelia using High-Fidelity Taq Polymerase Fusion (NEB). The fragment was subsequently ligated into the pMAL-c2X (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... the p27 allele and mVenus were synthesized as gBlocks (IDT) and cloned via Gibson assembly (NEB) into a modified pGIPz lentiviral expression vector (Open Biosystems) ...
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bioRxiv - Cancer Biology 2023Quote: ... the p27 allele and mVenus were synthesized as gBlocks (IDT) and cloned via Gibson assembly (NEB) into a modified pGIPz lentiviral expression vector (Open Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... SNAP-surface-Alexa549 (NEB) was applied to label surface SNAP-Fzd5 for 15 min at RT in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... or SNAP-surface-Alexa488 (NEB) were added at the secondary antibody staining stage ...
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bioRxiv - Cell Biology 2022Quote: ... SNAP-Surface 594 (NEB, #S9134S) in the following abbreviated as BG-ATTO594 and SNAP-Cell 647-SiR (NEB ...
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bioRxiv - Microbiology 2021Quote: ... The open reading frame (lacking the putative lipoprotein signal sequence) of each gene was PCR amplified using Q5 Hot Start Master Mix (New England Biolabs). Each PCR fragment ...
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bioRxiv - Cell Biology 2023Quote: ... Receptors were surface labeled by addition of SNAP-Surface Alexa Fluor 546 (1:1000, New England Biolabs) to DMEM F12 without serum and phenol red (21041-025 ...
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bioRxiv - Cell Biology 2019Quote: ... or SNAP-Surface Alex Fluor 647 (NEB), as appropriate ...
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bioRxiv - Biophysics 2022Quote: ... SNAP-Surface ATTO488 (S9124S, New England Biolabs), Tetraspeck Microspheres 0.2 µm (T7280 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and SNAP-surface dye 647 (NEB, #S9102S) were purchased from Sigma Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... All proteins were purified and labeled with BG-surface Alexa 647 and BC-surface Dy547 dyes (New England Biolabs) as previously described (Murayama and Uhlmann ...
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bioRxiv - Developmental Biology 2022Quote: ... and SNAP-Surface Block at 13 μM (NEB) for 20 min at 25°C (for intracellular labeling ...
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bioRxiv - Neuroscience 2020Quote: ... 1:1000 concentrations of SNAP-Surface 488 (NEB #S9124 ...
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bioRxiv - Biophysics 2021Quote: ... SNAP labelling (SNAP-Surface® 549, S9112S, NEB) of capping protein-beta and Rng2 1-189 was performed as per the manufactures protocol.
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bioRxiv - Biochemistry 2023Quote: ... and labelled using SNAP-Surface AlexaFluor 488 (NEB) using manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: SNAP-Surface® Alexa Fluor® 488 (NEB) were dissolve in DMSO to yield a labeling stock solution of 1 mM tags’ substrate ...
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bioRxiv - Biophysics 2023Quote: ... Purified SNAP-CP was incubated with 5x excess of SNAP-surface-549 dye or SNAP-surface-649 dye (New England Biolabs, USA) overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... and SNAP-Surface Alexa Fluor 647 (NEB, catalog S9136S) at 37 °C/ 5% CO2 for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... and SNAP-Surface 647 (1 μM, New England Biolabs) for 60 minutes at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1M cells were labeled with SNAP-Surface 647 (NEB) following manufacturer’s recommendation (50 μM concentration of SNAP-Surface 647 in complete cell media ...
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bioRxiv - Cell Biology 2019Quote: ... or SNAP-Surface Alexa Fluor647 (New England Biolabs S9136S), respectively ...
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bioRxiv - Biophysics 2020Quote: ... permeabilised and stained with the SNAP-Surface AlexaFluor647 (NEB) at 4 µM for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... SNAP-Surface Alexa Fluor 647 (S9136S, New England Biolabs), SNAP-Surface ATTO488 (S9124S ...
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bioRxiv - Molecular Biology 2022Quote: ... and 8 µM BC-647 (CLIP-Surface 647, NEB) in a base solution of Passive Lysis Buffer was rotated overnight at 4°C to label CLIP-tagged proteins ...
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bioRxiv - Cell Biology 2022Quote: ... 50 μM of SNAP-Surface Alexa Fluor 647 (NEB) was added and incubated for another 30 min on ice to saturate the remaining SNAP-tag binding sites ...
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bioRxiv - Immunology 2019Quote: ... cells were labeled with CLIP-Surface 547 (NEB, catalog S9233S) and SNAP-Surface Alexa Fluor 647 (NEB ...
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bioRxiv - Biophysics 2022Quote: ... and 2 µM of SNAP-Surface Alexa Fluor 647 (NEB) in extracellular buffer for 30 minutes at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... The benzylguanine (BG)-conjugated AF647 (SNAP-Surface; NEB; cat.# S9136S) was diluted to 1 μM in blocking solution (0,5% (w/v ...
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bioRxiv - Biophysics 2023Quote: SNAP-Surface BG-AF647 (New England Biolabs, cat. no. S9136S)
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bioRxiv - Immunology 2022Quote: ... 5 mol% labelled with SNAP-Surface Alexa Fluor 488 (NEB, S9128S), to 25% v/v HeLa lysate in a final volume of 20 µl (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2022Quote: ... and CLIP-tag ligands (CLIP surface 647 - BC 647 [NEB, S9234S]) at final concentrations of 1 μM in 0.3% PBT ...
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bioRxiv - Biochemistry 2023Quote: ... and SNAP-Surface® Alexa Fluor® 647 (New England Biolabs). Protein (5 µM ...
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bioRxiv - Biophysics 2020Quote: ... The fluorescent probe SNAP-Surface Alexa Fluor 546 (#S9132S) was from NEB Biotech ...
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bioRxiv - Cell Biology 2019Quote: ... Shi-SNAP was labeled fluorescently with SNAP-Surface 488 (New England Biolabs), and Shi-SNAP-Surface 488-mediated actin bundles were visualized by TIRF imaging ...
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bioRxiv - Cell Biology 2020Quote: ... and then labeled with SNAP-surface-549 (New England Biolabs, Ipswich, MA) overnight at 4°C following the manufacturers’ protocols ...
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bioRxiv - Biochemistry 2020Quote: ... by SDS-PAGE after labeling with SNAP-Surface 549 (New England Biolabs). The strains had doubling times similar to their parental strains YF702 or YF4 (Fig ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were labeled with 500nM SNAP-Surface 649 (New England Biolabs, #S9159S) for 5 minutes at 37°C for TIR-FM imaging or 1μM permeable SNAP-Cell 647-SiR (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... cells were labelled in suspension with SNAP-Surface 549 (New England Biolabs) for 30 minutes at 37°C and FACS-sorted to select for SNAP-GLP-1R-expressing ones ...
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bioRxiv - Biophysics 2024Quote: ... SNAP-Surface Alexa Fluor 647 (catalog no. S9136S) was obtained from NEB. 40 nm and 90 nm diameter gold nanoparticles (catalog no ...
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bioRxiv - Microbiology 2024Quote: ... beads were incubated with 10 μM SNAP-Surface Alexa Fluor 488 (NEB) for 1 hour at 4°C prior to elution ...
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bioRxiv - Cell Biology 2024Quote: ... Transfected cells were labeled with 1 µM SNAP-Surface 674 dye (NEB) for 30min followed by washing two times for 10 min with 300 µl MES300 per well ...
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bioRxiv - Neuroscience 2019Quote: ... 1 µM NGFR121W-SNAP was coupled with 3 µM BG549 surface (NEB # S9112) for 1 hour at 37°C in calcium imaging (CIB ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were labelled in full media with SNAP-Surface 549 (New England Biolabs) for 30 minutes at 37°C and imaged in RPMI without phenol red in a Nikon Eclipse Ti spinning disk confocal microscope with an ORCA-Flash 4.0 camera (Hamamatsu ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were labelled with 1 μM SNAP-Surface 549 (New England Biolabs) for 15 minutes at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with SNAP-Surface® 549 (DY549P1) (S9112S, New England Biolabs) for 15 min in a 37° incubator with 5%CO2 and sorted for the fluorescent signal.
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bioRxiv - Cell Biology 2020Quote: ... dCas9-SNAP protein samples were incubated with SNAP-Surface Alexa Fluor 647 (NEB S9136) at 37°C for 30 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... 1:1000 concentrations of SNAP-Surface 488 (NEB #S9124, New England Biolabs, Ipswich, MA), TMR Halo (G8252 ...
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bioRxiv - Bioengineering 2019Quote: Deglycosylation of yeast surface displayed tau was performed using PNGase (New England Biolabs (NEB), Cat ...