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Citations for New England Biolabs :
251 - 300 of 3865 citations for Borrelia burgdorferi sensu stricto B31 CRASP 1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Experiments with PURExpress in vitro protein synthesis kit (NEB, E6800) were performed as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The Cas9nls protein was obtained from NEB (cat number M0646T).
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bioRxiv - Synthetic Biology 2022Quote: We used PURExpress In Vitro Protein Synthesis Kit (NEB, E6800L) and the reaction was set up with the following manufacturer’s protocol ...
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bioRxiv - Systems Biology 2019Quote: ... 100 ng of T4 gene 32 protein (New England Biolabs), and 3× PrimeScript enzyme mix (Cat# RR037A ...
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bioRxiv - Molecular Biology 2020Quote: ... Cas9 protein (IDT) was suspended with a Diluent B (NEB) to make 1 μM solution ...
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bioRxiv - Synthetic Biology 2020Quote: We analyzed protein digests of purified ribosome (New England BioLabs) and purified LacZ (FUJIFILM Wako Pure Chemical Corporation ...
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bioRxiv - Developmental Biology 2019Quote: ... Blue pre-stained protein standard (11-190kDa) (New England Biolabs) was used ...
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bioRxiv - Genomics 2020Quote: ... The APOBEC3A protein was produced by (NEB E7133, Ipswich, MA).
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bioRxiv - Biophysics 2020Quote: ... Fusion proteins were purified in batch by amylose affinity (NEB), eluting in buffer B (buffer A with 0.02% DDM ...
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bioRxiv - Microbiology 2021Quote: ... and Nico (λDE3) with reduced histidine-rich background proteins (NEB). The RNase I precursor contains a signal peptide (amino acid residues 1-23 ...
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bioRxiv - Immunology 2021Quote: ... These two proteins were also treated with PNGase-F (NEB) under the reduced condition to remove N-glycans and loaded on the gel to assess the impact of the glycans on the protein size ...
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bioRxiv - Cell Biology 2021Quote: ... Protein A magnetic beads (New England Biolabs, Ipswich, MA, USA) were then added to the cell lysate and antibody mixture ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant hACE2 protein was digested using EndoH (New England Biolabs) and thrombin (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... and 2 μL of lambda protein phosphatase (NEB, Catalog #P0753S) were added to the washed FRQ-coupled V5 resin ...
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bioRxiv - Microbiology 2022Quote: ... the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs) was used to translate the synthetic transcriptome described above ...
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bioRxiv - Immunology 2022Quote: ... recombinant proteins were treated with PNGase F (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... A pre-stained protein standard (New England Biolabs, MA, USA) was used to estimate molecular weight ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were expressed in BL21(DE3) (New England Biolabs) in LB grown in 2 L baffled shake flasks ...
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bioRxiv - Microbiology 2023Quote: ... proteins were removed using the Monarch RNA Cleanup Kit (NEB) and 5’-mono-phosphorylated v51_mut_S templates were specifically digested with a terminator 5’-phosphate-dependent exonuclease (Biosearch technologies ...
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bioRxiv - Plant Biology 2023Quote: ... and Lambda Protein Phosphatase (Lambda PP) was purchased from NEB. After kinase reaction in kinase buffer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The unbound fraction was dephosphorylated using lambda protein phosphatase (NEB), calf intestinal phosphatase (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... and 5 µl of prestained protein ladder (New England Biolabs). The gel was electrophoresed at 150 V ...
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bioRxiv - Cell Biology 2023Quote: ... in the presence of protein kinase buffer (New England Biolabs) with 3 μCi [γ-32P]ATP ...
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bioRxiv - Cell Biology 2023Quote: ... kit and co-injected with Cas9 protein (New England Biolabs) into one-cell stage zebrafish eggs ...
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bioRxiv - Microbiology 2024Quote: ... 50 μL of protein G beads (New England Biolabs (NEB), S1430S ...
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bioRxiv - Microbiology 2024Quote: ... 50 μL of protein G beads (New England Biolabs (NEB), S1430S ...
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bioRxiv - Cell Biology 2024Quote: ... STAT1 proteins were dephosphorylated by lambda phosphatase (NEB, Ipswich MA) followed by the addition of protease and phosphatase inhibitors (Thermo Fisher) ...
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bioRxiv - Biochemistry 2022Quote: ... purified KRAS protein was mixed with GMP-PNP (molar ratio of 10:1 GMP-PNP:KRAS) and calf intestinal alkaline phosphatase (NEB cat# M0290, 3 units per mg of KRAS). The reaction mixture was incubated for 3 hours at room temperature and then purified by size-exclusion chromatography on a 10/300 Superdex 75 GL column (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... The protein dephosphorylation was performed on total WT and MLP knockout heart lysates by using Alkaline Phosphatase (rSAP)(M0371S NEB, 1U of rSAP/10mg of protein). The protein lysate containing phosphatases ...
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bioRxiv - Microbiology 2020Quote: ... The soluble proteins were mixed with amylose resin (New England Biolabs) according to the manufacturer’s protocols.
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bioRxiv - Molecular Biology 2020Quote: The protein was expressed in NiCo21 (DE3) cells (New England Biolabs) grown in Overnight Express Instant TB Medium (EMD Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Maltose Binding Protein monoclonal antibody IgG2a (New England Biolabs), mouse anti-Histone H3 [Trimethyl Lys9] 6F12-H4 (Novus Biologicals) ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with DTT and proteins were degraded using proteinase K (NEB). Total cellular RNA was purified using the RNeasy maxi kit (QIAGEN ...
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bioRxiv - Developmental Biology 2021Quote: ... Cas9 protein (New England Biolabs, #M0646M, EnGen Cas9 NLS 20 μM) and 300 pg of sgRNAs along with 5 ng of GFP cRNA were co-injected into the X ...
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bioRxiv - Developmental Biology 2021Quote: ... in equimolar ratios and EnGen®Spy Cas9 NLS protein (NEB) into 1-cell zebrafish embryos.
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bioRxiv - Immunology 2021Quote: ... Deglycosylation was performed on beads using Protein Deglycosylation Mix II (NEB) in denaturing conditions according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... associated with EnGen™ Spy Cas9 NLS protein (New England BioLabs), using the 4D-Nucleofector™ System (Lonza ...
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bioRxiv - Physiology 2020Quote: ... were co-injected with 0.5 μl Cas9 protein (NEB, M0386, 20μM), diluted 1:10 ...
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bioRxiv - Cell Biology 2019Quote: ... MBP-MKLP1 proteins were purified on amylose resin (New England Biolabs), washed 5 times with Washing Buffer (50 mM Tris-HCl pH 7.5 ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... MBP-fusion proteins were purified using Amylose resin (New England Biolabs), washed in lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Molecular weights were identified using a protein standard (BioLabs, Cat# P7712S). Beta-actin was used as the loading control ...
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bioRxiv - Biochemistry 2020Quote: ... morsitans salivary proteins were treated with PNGase F (New England Biolabs), resolved by SDS-PAGE and transferred onto a PVDF membrane (GE Healthcare ...
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bioRxiv - Bioengineering 2021Quote: Recombinant RfxCas13d proteins were expressed in E.coli NiCo21(DE3) (NEB C2529). Cells were grown in 1L of lysogeny (Luria-Bertrani ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were then coupled to protein G beads (New England Biolabs). After beads were washed ...
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bioRxiv - Plant Biology 2020Quote: ... the purified proteins (0.125 mg) or RNase If (1.25 U; NEB) were incubated at 25°C for 60 minutes with a fluorescent-labeled RNA substrate (5’-fluorescein isothiocyanate (FITC)-CUUUUAG(A20) ...
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bioRxiv - Molecular Biology 2022Quote: ... and α1-3,4 fucosidase (New England Biolabs, 4 U/μg protein). All enzymatic reactions were performed as a 1-step reaction with 1x Glycobuffer 2 (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... Molecular weights were determined using protein standards (New England Biolabs P7719).
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bioRxiv - Molecular Biology 2021Quote: ... The protein was then digested with LysC endoproteinase (0.25 µg; NEB) for 4 hours at 37°C ...
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bioRxiv - Biochemistry 2019Quote: ... MBP fusion proteins were purified on amylose beads (New England Biolabs) in column buffer (20mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was first bound to amylose resin (New England Biolabs). Afterwards ...