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Citations for New England Biolabs :
501 - 550 of 997 citations for Anti PI3 kinase p150 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: Assays were conducted by incubating 0.1 and 1 μM SidJ in 1X Protein Kinase buffer (NEB), with 10 mM CaCl2 ...
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bioRxiv - Microbiology 2019Quote: ... adjacent primers containing the mutation of interest were 5′-phosphorylated using T4 polynucleotide kinase (NEB, M0201S) using the buffer from the T4 DNA ligase ...
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bioRxiv - Genetics 2019Quote: ... and phosphate groups added to the 5’ends using T4 Polynucleotide Kinase (New England Biolabs, M0201S) by first incubating for 30 minutes without ATP and then 30 min with ATP to remove and add the phosphate group ...
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bioRxiv - Microbiology 2021Quote: ... mutagenic oligonucleotides for each CDR were mixed and phosphorylated by T4 polynucleotide kinase (New England BioLabs) in 70mM Tris–HCl (pH7.6) ...
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bioRxiv - Cell Biology 2021Quote: ... The complexes on the beads were incubated with 40 units T4 Polynucleotide kinase (New England Biolabs), first with P32 labelled ATP for 45 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Annealed oligonucleotides containing the homology region were phosphorylated with T4 polynucleotide kinase (New England Biolabs, M201) and then ligated into the Bbs1 site of the vectors pX335 and pKN7 (Appendix Tables S1 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1µL of each Fw and Rv were annealed and phosphorylated with T4 Polynucleotide Kinase (PNK, NEB) using the following program ...
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bioRxiv - Molecular Biology 2019Quote: ... a master mix was prepared containing 2 µl 10x T4 polynucleotide kinase buffer without ATP (NEB) and 1 µl murine RNase inhibitor per sample and 3 µl were added to each sample together with 2 µl truncated T4 polynucleotide kinase (#M0201 ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein-RNA complexes were radiolabeled on-bead in 40 µl reactions with T4 polynucleotide kinase (NEB) and 2 µl [γ-32P]ATP (6,000 Ci/mmol ...
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bioRxiv - Biophysics 2020Quote: ... and were 5’ end-radiolabeled with [Ɣ-32P]-ATP using 10 U T4 polynucleotide kinase (NEB). Radiolabeled transcripts were purified on polyacrylamide gel (6% acrylamide:bisacrylamide 19:1 ...
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bioRxiv - Microbiology 2019Quote: ... phosphorylated by the T4 Polynucleotide Kinase and then incubated with T4 DNA Ligase (New England Biolabs) and transformed in E ...
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bioRxiv - Microbiology 2019Quote: ... for each sample 3 μg of DNase-digested RNA was treated with T4 Polynucleotide Kinase (NEB) for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2019Quote: ... the 3’ ends of RNA fragments were dephosphorylated with T4 polynucleotide kinase (New England BioLabs #M0201S). A preadenylated DNA adaptor sequence was ligated to the 3’-hydroxyl ends of the RNA fragments using T4 RNA Ligase (T4 RNA Ligase 2 ...
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bioRxiv - Microbiology 2021Quote: ... T4 polynucleotide kinase (T4 PNK) used in EMSAs and all other enzymes were purchased from NEB and used according to manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... denatured and phosphorylated with 10 U of 3’-phosphatase-minus T4 polynucleotides kinase (New England Biolabs) at 37 °C for 30 min ...
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bioRxiv - Genomics 2023Quote: ... the DNA was treated in a 30 µL reaction containing 12 U T4 polynucleotide kinase (NEB), 1 mM ATP (NEB) ...
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bioRxiv - Genetics 2023Quote: ... All probes were 5’ end-labeled with [alpha-32P]-ATP and T4 polynucleotide kinase (NEB Inc.). The gels were exposed to a PhosphorImager ...
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bioRxiv - Molecular Biology 2023Quote: ... RNAI was detected by a DNA probe (JW198) labelled with T4 polynucleotide kinase (New England Biolabs).
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bioRxiv - Microbiology 2024Quote: ... or INRKKR cRNA/mRNA (5’-CATGATTGTGTCAACCTGCTCTG) were radioactively labelled using T4 polynucleotide kinase (New England Biolabs) and ɣ-32P-ATP (PerkinElmer) ...
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bioRxiv - Genetics 2024Quote: ... All probes were 5’ end-labeled with [alpha-32P]-ATP and T4 polynucleotide kinase (NEB Inc.). The gels were exposed to a Phosphor Imager ...
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bioRxiv - Microbiology 2019Quote: ... and detected using anti-MBP antibodies (New England Biolabs).
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bioRxiv - Developmental Biology 2019Quote: ... incubated overnight with anti-SNAP antibody (NEB, Ipswich, MA), washed and probed with goat-anti-rabbit horseradish peroxidase secondary antibody (Invitrogen ...
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bioRxiv - Developmental Biology 2019Quote: ... and detected using the anti-MBP-HRP antibody (NEB). The bound protein (~42kDa ...
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bioRxiv - Molecular Biology 2022Quote: Anti-M13-pIII monoclonal antibody (E8033S, New England Biolabs), sheep anti-mouse IgG (H/L):HRP (AAC10P ...
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bioRxiv - Plant Biology 2023Quote: ... after which an anti-p42/p44-erk antibody (NEB) was employed to detect the activated MAPKs on western blots.
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bioRxiv - Cell Biology 2023Quote: ... anti-SNAP-tag antibody (New England Biolabs, Ref. P9310S), anti-clathrin heavy chain antibody (BD Bioscience ...
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bioRxiv - Microbiology 2024Quote: ... and detected using anti-MBP antibodies (New England Biolabs). As a negative control ...
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bioRxiv - Microbiology 2021Quote: ... After dephosphorylation of the 3′ ends of the recovered RNA using T4 polynucleotide kinase (T4 PNK; NEB), RNA was ligated to Linker-1 (5′-App CTGTAGGCACCATCAAT ddC-d′ ...
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bioRxiv - Biophysics 2022Quote: ... The donor-labeled DNA was phosphorylated at the 5’ end using T4 Polynucleotide Kinase (New England BioLabs) for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were washed, treated with T4 Polynucleotide Kinase (PNK, with no 3’-phosphatase activity) (NEB, M0236S), and miRNA-RNA chimeras were produced with T4 RNA Ligase I (NEB ...
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bioRxiv - Microbiology 2019Quote: ... and phosphorylated for 30 minutes at 37 °C with 4U of T4 Polynucleotide Kinase (New England Biolabs) in a total volume of 20 µl according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... These peptides were used as the substrate in a PKA kinase assay kit (New England Biolabs, #P6000S) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... two oligos (see Supplementary Table 3) were annealed and 5’ phosphorylated (T4 Polynucleotide Kinase kit, M0201S, NEB) as described previously (LentiGuide-Puro and LentiCRISPRv2) ...
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bioRxiv - Bioengineering 2021Quote: ... 5′ phosphorylation was performed either during synthesis (for tRNAfMet) or following synthesis using T4 Polynucleotide kinase (NEB)(for tRNALys and tRNAPhe ...
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bioRxiv - Biochemistry 2020Quote: ... 8 μL of the de-phosphorylated DNA was incubated with 2 μL T4 Polynucleotide Kinase (NEB M0203S), 3 μL γ32P-dATP (Perkin Elmer) ...
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bioRxiv - Cancer Biology 2021Quote: ... End-repairing of the sheared DNA (using T4 DNA polymerase, T4 DNA polynucleotide kinase, Klenow (all NEB)) was followed by dATP addition (Klenow exo- ...
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bioRxiv - Molecular Biology 2021Quote: An RNA oligonucleotide (5’-GGCATGTGATTGGTGGGTC) was 5’ labelled with gamma 32P ATP by T4 Polynucleotide Kinase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... These peptides were used as the substrate in a PKA kinase assay kit (New England Biolabs, #P6000S) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... oligos listed in Table S2 were purchased from IDT and phosphorylated using T4 Polynucleotide Kinase (M0201L; NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... In brief, size selected ribosome footprints were treated with T4 Polynucleotide Kinase (#M0201L, New England Biolabs (NEB)) followed by ligation to a DNA linker using T4 RNA ligase 2 ...
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bioRxiv - Plant Biology 2021Quote: ... The antisense strand with a 3′ phosphate was radiolabeled by T4 Polynucleotide Kinase (3’ phosphatase minus) (NEB) and [γ-32P]ATP ...
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bioRxiv - Microbiology 2020Quote: The 3′ ends of the ribosome footprint RNA fragments were then treated with T4 polynucleotide kinase (NEB) to allow ligation of a pre-adenylated DNA linker with T4 Rnl2(tr ...
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bioRxiv - Biochemistry 2020Quote: ... T4 polynucleotide kinase (T4 PNK) used in EMSAs and all other enzymes mentioned were purchased from NEB and used according to manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The minimal promoter oligos were phosphorylated and annealed using T4 Ligation Buffer and T4 Polynucleotide Kinase (NEB) at 37 °C for 30 minutes followed by 95 °C for five minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... 400 ng of eluted DNA fragments were end-labeled with T4 Polynucleotide Kinase (T4 PNK) (NEB M0201L) and 10 µCi of [α-32P]-ATP (Perkins Elmer NEG002A ...
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bioRxiv - Biochemistry 2021Quote: ... and 2 µl of 10x Promega T4 ligase buffer (contains ATP) with 1µl of PNK kinase (NEB) and incubated at 37°C for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: Radiolabeling of oligonucleotides was carried out for 1 h at 37 °C using T4 polynucleotide kinase (NEB), 0.25 μM oligonucleotide ...
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bioRxiv - Cell Biology 2022Quote: ... Hybridized oligonucleotides were phosphorylated with 5 U T4 polynucleotide kinase in T4 Ligation buffer (New England Biolabs) for 30 min at 37 °C ...
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bioRxiv - Genetics 2021Quote: ... The kinase-dead MELKT167A mutant was constructed using a Q5 site-directed mutagenesis kit (New England Biolabs) with 5′-aggattaccatctacaggcatgctgtgggagtctg-3′ forward and 5′-cagactcccacagcatgcctgtagatggtaatcct-3′ reverse primers.
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bioRxiv - Molecular Biology 2021Quote: ... Table 4 was end labeled using gamma-ATP and T4 Polynucleotide Kinase radioactive labeling protocol from NEB. Labelled oligos were purified using GE Healthcare illustra ProbeQuant G-50 Micro Columns and the membrane was probed overnight ...