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Citations for New England Biolabs :
151 - 200 of 9345 citations for Alkaline Phosphatase Colorimetric Activity Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA or immunoprecipitated RNA (MIWI or MILI) was de-phosphorylated with Shrimp Alkaline Phosphatase (M0371, NEB) and end-labeled using T4 polynucleotide kinase (M0201 ...
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bioRxiv - Bioengineering 2019Quote: pRM02-Spec was linearized by AgeI and simultaneously dephosphorylated by Shrimp Alkaline Phosphatase (New England Biolabs, Cat. # M0371S). The 4.6 kb PSF expression cassette was PCR amplified from pPSF using primer pair PSF-AgeI-For/ PSF-AgeI-Rev and double digested with DpnI/AgeI ...
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bioRxiv - Bioengineering 2019Quote: pRM01-Spec was linearized by AgeI and simultaneously dephosphorylated by Shrimp Alkaline Phosphatase (New England Biolabs, Cat. # M0371S). The RSLB1 expression cassette was PCR amplified from plasmid pRSLB1 using primer pair R32CerLBAgeIfor/newPCFAgeIrev (PCR product size ...
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bioRxiv - Molecular Biology 2022Quote: ... Approximately 3-5μg of native genomic DNA were dephosphorylated with 0.05U/μl of shrimp alkaline phosphatase (rSAP) (New England BioLabs) to reduce the amount of off-target molecules in the final SMRTbell® library ...
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bioRxiv - Molecular Biology 2020Quote: ... the amplified products were cleaned using Exonuclease-Shrimp Alkaline Phosphatase (Exo-SAP) mixture (New England Biolabs, Ipswich, Massachusetts) and sequenced bidirectionally using BigDye v3.1 terminator kit in ABI 3500XL Genetic Analyzer (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... pWPI Puro was digested by PmeI and dephosphorylated using 20 U of calf alkaline phosphatase (New England Biolabs) for 1 h at 37°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Amplified PCR product was cleaned prior to cycle sequencing by exonuclease I/shrimp alkaline phosphatase (New England Biolabs) and used for bi-directional Sanger sequencing on an ABI 3730 automated sequencer (MCLAB) ...
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bioRxiv - Cell Biology 2022Quote: Size markers 19 and 35 nucleotides long were dephosphorylated using 0.5 U/ μl CIP alkaline phosphatase (NEB M0290L) by incubating at 37°C for 15 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... and treated with alkaline phosphatase and then annealed protospacer sequences were cloned in using T4 DNA ligase (NEB). gRNAs were cloned into the pU6-Cj-sgRNA expression plasmid (Addgene #89753 ...
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bioRxiv - Microbiology 2023Quote: ... plasmid was pre-treated with Alkaline phosphatase and amplicons were treated with T4 polynucleotide kinase (New England Biolabs). Cloned plasmids were subjected to colony PCR using Taq polymerase (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The backbones of pKB11 and pKB12 were treated with calf intestinal alkaline phosphatase (CIP) (New England Biolabs, M0525S) to prevent self ligation ...
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bioRxiv - Bioengineering 2019Quote: ... plasmid pRM03-Spec was linearized by AgeI and simultaneously dephosphorylated by Shrimp Alkaline Phosphatase (New England Biolabs, Cat. # M0371S). A 4.2 kb PCM expression cassette was PCR amplified form plasmid pPCM using primer pair MMorCF-AgeIfor/newPCFAgeIrev and double digested with DpnI/AgeI ...
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bioRxiv - Microbiology 2020Quote: ... 15 μg of DNA-depleted RNA was incubated with 25 units of calf intestinal alkaline phosphatase (CIP) (#M0290, NEB) in a 50 μl reaction volume for 1 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... RNAs were precipitated in cold ethanol and 0.3 M of sodium acetate and dephosphorylated using Calf-intestinal alkaline phosphatase (New England Biolabs), according to manufacturer protocol ...
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bioRxiv - Microbiology 2019Quote: ... all restriction enzyme digested plasmids were treated with Calf Intestinal Alkaline Phosphatase (New England Biolabs, Inc., Cat. No. M0290S) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Following this, 2μL Exonuclease 1 (20U/μL, Enzymatics X8010L) and 1μL Shrimp Alkaline Phosphatase (rSAP) (1U/μL, NEB M0371L) was added to each reaction followed by vortexing and incubation in a thermocycler at 37°C for 30min followed by 4°C forever.
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bioRxiv - Biochemistry 2023Quote: ... The 5’ ends of the CRISPR leader and array fragments were dephosphorylated using Quick Calf Intestinal alkaline Phosphatase (NEB), and the DNAs were purified away from protein using a DNA Clean & Concentrator kit (Zymo Research) ...
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bioRxiv - Genomics 2023Quote: ... Following this, 2μL Exonuclease 1 (20U/μL, Enzymatics X8010L) and 1μL Shrimp Alkaline Phosphatase (rSAP) (1U/μL, NEB M0371L) was added to each reaction followed by vortexing and incubation in a thermocycler at 37°C for 30min followed by 4°C forever.
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... 50 pmol of the in-vitro -transcribed RNA were dephosphorylated using 25 U of calf intestine alkaline phosphatase (NEB) in a 50 µL reaction volume ...
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bioRxiv - Microbiology 2023Quote: ... followed by a dephosphorylation step using the shrimp alkaline phosphatase (Phusion High-fidelity DNA polymerase, New England Biolabs, France). hcp and tssB flanking regions were PCR-amplified from CFBP13503 with the Phusion High-Fidelity DNA polymerase (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was stopped by incubating at 65°C for 5 min followed by addition of 5 mM MgCl2 and 0.5 units of Shrimp Alkaline Phosphatase (rSAP, NEB M0371S). The phosphatase reaction was incubated at 37°C for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were rinsed three times with NP40 lysis buffer and treated with 0.5 U/μL calf intestinal alkaline phosphatase (New England BioLabs, M0290) in dephosphorylation buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... The RNA bound to Gag was first dephosphorylated at the 5’ end with calf intestinal alkaline phosphatase (New England BioLabs, NEB) followed by T4 polynucleotide kinase-catalyzed (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... This fragment was ligated into pGEM-3Zf (+) that had been digested with BamHI and SalI and dephosphorylated with calf intestinal alkaline phosphatase (NEB). 6XHis tag was added to the N-terminus of Upf1 in pGEM-3Zf (+ ...
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bioRxiv - Microbiology 2020Quote: ... The pKAS32 vector was restriction digested using SacI and XbaI at 37°C for 1 hour followed by an additional 30 minutes at 37°C with alkaline phosphatase from calf intestine (CIP) (New England Biolabs). Vector backbone and upstream and downstream segments were joined using Gibson assembly (New England Biolabs ...
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bioRxiv - Immunology 2022Quote: ... Beads were washed and resuspended in NEBuffer 3 containing Calf Intestinal Alkaline Phosphatase at a concentration of 0.5 U/μl (NEB, M0290) to dephosphorylate the RNA ...
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bioRxiv - Evolutionary Biology 2022Quote: ... These radioactively labeled RNAs were prepared by mixing 1 μM of RNA with 1X of Shrimp Alkaline Phosphatase buffer (rSAP reaction buffer, New England BioLabs), 1 U/μL of rSAP enzyme (New England BioLabs ...
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bioRxiv - Molecular Biology 2023Quote: Radioactive labelling of the in-vitro-transcribed RNA was carried out by first dephosphorylating 50 pmol of RNA with 25 U of calf intestine alkaline phosphatase (NEB) in a 50 µL reaction and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... Gene inserts were cloned into linearized pWSK29 (46) sequentially digested with the appropriate restriction endonucleases and de-phosphorylated with Shrimp Alkaline Phosphatase (New England Biolabs). Inserts were ligated into the linearized plasmid overnight at 16°C using T4 DNA ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’ triphosphates on the synthesis products were converted into 5’ monophosphates: samples were treated with recombinant shrimp alkaline phosphatase (NEB) (10 µl reaction in 1× NEB buffer 2.1 ...
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bioRxiv - Microbiology 2023Quote: Lysates of HEp-2/ι1hUNGs cells mock infected or infected with wild-type HSV-1(F) at an MOI of 10 for 24 h were treated with alkaline phosphatase (CIP) (New England BioLabs) as described previously48.
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bioRxiv - Bioengineering 2023Quote: ... Phosphorylated and annealed arrays were then cloned into the respective Esp3I-digested and alkaline phosphatase treated crRNA cloning backbone (described above) using T4 DNA ligase (NEB). crRNA arrays were verified by Sanger sequencing ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR amplicons were run by electrophoresis on a 2% agarose gel to ensure the absence of primer-dimers and PCR samples for each primer pair-opsin target were purified using Exonuclease (EXO) and Shrimp alkaline phosphatase (SAP) (NEB) prior to Sanger Sequencing to confirm gene-specific amplification ...
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bioRxiv - Microbiology 2023Quote: ... 50 pmol of each RNA were dephosphorylated by incubation for 1 h at 37°C with 25 U of calf intestine alkaline phosphatase (NEB) in a final volume of 50 µL ...
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bioRxiv - Microbiology 2023Quote: Lysates of U2OS cells infected with wild-type HSV-2 186 at an MOI of 3 for 24 h were treated with calf intestinal alkaline phosphatase (CIP) (New England BioLabs) as described previously60.
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bioRxiv - Biophysics 2023Quote: ... Restriction digestion of the pLentiCRISPRv2 vector was performed using FD-Esp3I (Thermo) and dephosphorylation was done later using the Shrimp alkaline phosphatase (rSAP, NEB). An overnight ligation was performed by mixing digested vector and annealed gRNA inserts using T4 DNA ligase (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were washed, treated with T4 Polynucleotide Kinase (PNK, with no 3’-phosphatase activity) (NEB, M0236S), and miRNA-RNA chimeras were produced with T4 RNA Ligase I (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... nucleoplasm fractions (100 µg) were incubated with either mock (distilled water) or 50 Units of calf intestinal alkaline phosphatase (QuickCIP, NEB M0525L) for 1 hour at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... nucleoplasm fractions (100 µg) were incubated with either distilled water (mock) or 50 Units of calf intestinal alkaline phosphatase (QuickCIP, NEB M0525L) and brought up to 100 uL with PBS ...
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bioRxiv - Microbiology 2019Quote: ... The RNA bound to Gag was first dephosphorylated at the 5’ end with calf intestinal alkaline phosphatase (New England BioLabs, NEB) followed by T4 polynucleotide kinase-catalyzed (NEB ...
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bioRxiv - Genomics 2022Quote: ... Approximately 3 μg of input DNA was dephosphorylated with alkaline phosphatase (ONT, cat SQK-CS9109) in CutSmart Buffer (NEB, cat B7204). Following enzyme inactivation of alkaline phosphatase ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated at 37°C for 20 min followed by dephosphorylation with 1 U Shrimp Alkaline Phosphatase (rSAP, New England Biolabs, #M0371S) and incubation for another 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... 10 mM MgCl2 and 1 mM DTT) and treated with 0.5 U/mL calf intestinal alkaline phosphatase (New England Biolabs, cat#M0290) in dephosphorylation buffer for 10 minutes at 37 ℃ ...
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bioRxiv - Molecular Biology 2024Quote: ... 250 pmol of each RNA oligo was treated with 10 units calf intestinal alkaline phosphatase (CIP, New England Biolabs Inc., M0290S) in 37°C for 30 minutes ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We cleaned samples in preparation for sequencing using Shrimp Alkaline Phosphatase (USB, Swampscott, MA) and Exonuclease I (New England Biolabs, Ipswich, Maryland) and cycle sequenced in the forward and reverse direction using an ABI 3730 DNA Sequencer (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: Linker ligation at 3’ ends was set up per sample after washes following alkaline phosphatase treatment by preparing a T4 RNA ligase 1 (NEB, cat. #M0204S) reaction in 40μL following the manufacturers’ instructions with 100pmol of radiolabeled L32 RNA linker ...
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bioRxiv - Cell Biology 2022Quote: ... 15 μL of cell lysate was further treated for 1 h at 37°C with 40 units of Quick calf intestinal alkaline phosphatase (CIP, New England Biolabs, Ipswich, MA, USA) per the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... WarmStart Colorimetric LAMP 2X Master Mix (NEB) was used with 0.4 μM SYTO-9 (Thermo Scientific).
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bioRxiv - Genetics 2019Quote: ... phosphatase treated (Antarctic phosphatase, NEB M0289S), and ligated together using T4 ligase (NEB ...