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Citations for New England Biolabs :
51 - 100 of 2127 citations for Adenovirus Type 5 Particles CMV β galatosidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... These SETD3 mutants were introduced into either EcoRV-digested pLenti-CMV-Puro-Dest (w118-1) or EcoRV-digested pLenti-CMVTRE3G-Puro-DEST (w811-1) by Gibson Assembly (New England Biolabs).
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bioRxiv - Biophysics 2022Quote: ... The SETD3 V266D mutant was introduced into EcoRV-digested pLenti-CMV-Puro-Dest (w118-1) by Gibson Assembly (New England Biolabs). The SETD3 V266D construct was then used as a template to sequentially incorporate Q257A and Y288P mutations by Phusion site-directed mutagenesis using the following primer pairs ...
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bioRxiv - Biophysics 2022Quote: ... This SETD3 mutant was introduced into EcoRV-digested pLenti-CMV-Puro-Dest (w118-1) by Gibson Assembly (New England Biolabs).
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bioRxiv - Biophysics 2022Quote: ... These SETD3 mutants were introduced to either EcoRV-digested pLenti-CMV-Puro-Dest (w118-1) or EcoRV-digested pLenti-CMVTRE3G-Puro-DEST (w811-1) by Gibson Assembly (New England Biolabs).
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bioRxiv - Biophysics 2022Quote: ... These SETD3 mutants were introduced to either EcoRV-digested pLenti-CMV-Puro-Dest (w118-1) or EcoRV-digested pLenti-CMVTRE3G-Puro-DEST (w811-1) by Gibson Assembly (New England Biolabs).
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bioRxiv - Biophysics 2022Quote: ... The SETD3 G361R mutant was introduced into EcoRV-digested pLenti-CMV-Puro-Dest (w118-1) by Gibson Assembly (New England Biolabs). The SETD3 G361R construct was then used as a template to incorporate F409A or F409A and N413A mutations by Phusion site-directed mutagenesis using the following primer pairs ...
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bioRxiv - Neuroscience 2021Quote: ... and the product was inserted into a lentiviral expression vector (pSIH-H1) containing CMV promotor using NEBbuilder HIFI kit (NEW ENGLAND Biolabs). To co-express GFP-actin and mCherry-KDEL ...
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bioRxiv - Cell Biology 2019Quote: ... N-cadherin-EGFP-αE-catenin fusions were moved in to pShuttle-CMV using NEBBuilder HiFi DNA Assembly Master Mix (New England Biolabs). Positive clones were transformed into AdEasier E ...
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bioRxiv - Microbiology 2021Quote: ... The fragment of sNTurboID was PCR amplified from pLX304 CMV FKBP-V5-sTurboID (N) and cloned into the pcDNA5-VP35-HA using NEBuilder HiFi DNA Assembly Master Mix (NEB). The fragment of VP35-sNTurboID ...
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bioRxiv - Microbiology 2023Quote: ... pLENTI-CMV-GPRC5A was mutated to remove m6A sites (A57G, G120T, C174T, A264G) using Q5 site directed mutagenesis kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... A cDNA clone for the 2b protein of Ho-CMV was recapitulated by site-directed mutagenesis of the coding sequence of the Fny-CMV 2b protein using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). The resultant DNA product encoding the recapitulated Ho-CMV 2b protein contained the amino acid substitutions S47A ...
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bioRxiv - Cell Biology 2023Quote: ... flag tag and SunTag reporter were cloned from the SINAPs construct to replace the CMV promoter and tdTomato reporter of the tdTomato-FL2 plasmid using NEBuilder HiFi DNA Assembly technology (NEB).
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bioRxiv - Neuroscience 2023Quote: ... the CMV PSAM4 GlyR WPRE BGH pA plasmid was subjected to an overnight restriction digestion reaction using BsaI-HF v2 (NEB). The restriction reaction products were run on a gel ...
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bioRxiv - Cell Biology 2024Quote: ... or N-terminus of PEmax (PEmax-hP53DD-P2A-hMLH1dn) under the constitutive expression from a CMV promoter using Hifi DNA assembly (NEB). To generate the other two all-in-one prime editor with linkages in between the three components ...
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bioRxiv - Immunology 2022Quote: ... Particle titers were determined by isolation of genomic RNA and quantitation by RT-qPCR (NEB # E3031) using an Applied Biosystems vii7A™ Real-Time PCR system ...
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bioRxiv - Genomics 2022Quote: ... PIP particles were suspended in buffer with 29 U/mL Proteinase K Proteinase K (NEB #P8107S) and 70 mM DTT (Sigma #D9779 ...
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bioRxiv - Microbiology 2023Quote: ... cell and virus particle lysates were treated with PNGase F and Endo Hf (New England Biolabs) following the manufacturer’s protocol for 1 hour at 37°C prior to Western blotting.
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bioRxiv - Molecular Biology 2020Quote: ... and digested overnight with <ι>β-agarase (NEB M0392). DNA combing was performed using commercially available coverslips (Genomic Vision COV-001) ...
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bioRxiv - Biochemistry 2022Quote: ... coli strain NEB 10-β competent cells (New England Biolabs) and plated on LB agar containing 50 μg/mL kanamycin ...
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bioRxiv - Immunology 2019Quote: ... 2 μl of T4 bacteriophage β-glucosyltransferase (T4-BGT, NEB) and ultrapure water to a final volume of 30 μl ...
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bioRxiv - Molecular Biology 2023Quote: ... The agarose was destructed by incubation with β-agarase (NEB) overnight at 42’c and transferred to slides to stretch DNA fibers ...
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bioRxiv - Cell Biology 2023Quote: ... the agarose plugs were dissolved with β-agarase (NEB; M0392) enzyme according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Plugs were then dissolved with β-Agarase I (M0392S, NEB). Molecular combing was performed using the Molecular Combing System (Genomic Vision ...
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bioRxiv - Microbiology 2019Quote: ... All fragments contained ~20bp overhangs and were assembled into EcoRV cut pLenti CMV Puro DEST (w118-1) using NEBuilder® HiFi DNA Assembly Master Mix (New England BioLabs) or Gibson-like master mix (Berkeley MacroLabs ...
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bioRxiv - Cell Biology 2021Quote: ... Amplified cDNA fragments and HindIII-digested pFLAG-CMV-6a were subjected to the In-Fusion or NEBuilder reaction (New England BioLabs, E2621).
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bioRxiv - Genomics 2021Quote: ... Produced insert was cloned into the abovementioned pENTR vector from Twist Biosciences bearing GSH1 and GSH2 300bp homology arms as well as CMV promoter sequence using Gibson Assembly Master Mix (NEB, #E2611L). HDR donors were amplified from these plasmids using biotinylated primers with phosphorothioate bonds between the first 5 nucleotides on both 5’ and 3’ ends ...
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bioRxiv - Synthetic Biology 2022Quote: The PCR insert was assembled into 1600 ng of a linearized mRNA selection vector (AAV9-CMV-Express) with NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621L) at a 3:1 insert:vector Molar ratio in a 80 μL reaction volume ...
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bioRxiv - Molecular Biology 2022Quote: ... The plasmids for overexpressing the long and short isoforms of HERPUD2 were generated by cloning corresponding sequences into p3×Flag-CMV between the EcoRI and BamHI (NEB, R3136S) restriction sites ...
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bioRxiv - Biophysics 2020Quote: Recombinant wild-type human histone H1.0 (New England Biolabs, cat. # M2501S) was used for experiments with fluorescently-labeled nucleosomes ...
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bioRxiv - Synthetic Biology 2022Quote: ... and sRNA plasmids using BsaI Type IIS Enzyme (New England Biolabs) and transformed into E ...
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bioRxiv - Bioengineering 2023Quote: ... Remnant wild-type DNA was degraded via DPN1 (New England Biolabs) digestion for two hours at 37°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... the LMP agarose gel was hydrolysed using β-Agarase I (NEB) for 30 min at 42°C ...
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bioRxiv - Genomics 2023Quote: ... 1.56 µL of 500 µM β-Nicotinamide adenine dinucleotide (NAD+) (NEB), and 15U of E ...
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bioRxiv - Biochemistry 2024Quote: The DNA encoding full-length β-galactosidase was amplified from NEB Escherichia coli (E ...
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bioRxiv - Cell Biology 2022Quote: ... mTagBFP2 and Cas9-Avidin provided by Max Wilson into an expression vector bearing a CMV promoter and flanking PiggyBac transposase-compatible inverted terminal repeats using Gibson Assembly (New England BioLabs Inc., E2611L) according to supplier instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Amplicons were purified by agarose gel extraction and inserted into pLVX-CMV-MCS-10N-mPGK-puro at EcoRI and NotI sites by using NEBuilder HiFi DNA Assembly Master Mix (NEB Cat# E2621L). At least 3 individual E.coli clones were picked for plasmid extraction and plasmids were sequenced with CMV-Forward and mPGK-Reverse primers (See Key resource table ...
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bioRxiv - Biochemistry 2020Quote: ... m7G(5′)ppp(5′)G (NEB, #S1404S) and m7G(5′)ppp(5′)A (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... Golden Gate Assembly protocol was applied with type IIS endonuclease Esp3I (NEB). The product was transformed into competent E ...
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bioRxiv - Immunology 2023Quote: ... standard restriction cloning using the Type IIS restriction enzyme BsmBI-v2 (NEB) was employed to insert the leader peptides and variable regions into pVITRO1 (mouse IgG1 κ) ...
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bioRxiv - Genomics 2020Quote: ... Agarose was subsequently degraded by adding 2 μl of β-agarase (Biolabs). To stretch DNA fibers ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli strain DH10-β cells (45 μL each; C3019I, New England Biolabs) thawed on ice for 10 minutes and mixed gently by tapping ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were grown in DH10-β outgrowth medium (New England Biolabs, B9035S) for transformation ...
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bioRxiv - Microbiology 2023Quote: ... One unit of β-agarase I (New England Biolabs; Ipswich, Massachusetts, USA) and 10 µL of 10x reaction buffer was added to 100 μL of cell suspension and incubated at 42°C for 1.5 h ...
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bioRxiv - Physiology 2023Quote: ... Primary antibodies used targeted pan anti-β-hydroxybutyryllysine (PTM Biolabs, PTM-1201) or anti-amyloid-β1-16 (BioLegend 6E10 ...
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bioRxiv - Biochemistry 2020Quote: ... and m7G(5′)ppp(5′)A (NEB, #S1405S) also referred throughout the manuscript as N7-meGpppG and N7-meGpppA for consistency ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µL RNase H (5 U/µL, NEB), 2.5 µL RNase III (1 U/µL ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’-hydroxyl (5’HO-RNA30-FAM-3’) or 5’-Gppp (5’Gppp-RNA30-FAM-3’) in 1x NEBuffer 3 (NEB; B7003), in 20% denaturing polyacrylamide gels ...
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bioRxiv - Molecular Biology 2020Quote: The wild-type fragments were amplified with Phusion High-Fidelity DNA Polymerase (NEB) using HEK293T cDNA ...
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bioRxiv - Cell Biology 2019Quote: Purified Wss1 wild type (3.1 μM) was mixed with purified histone H3 (NEB) (3.3 μM ...
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bioRxiv - Synthetic Biology 2022Quote: ... 10,000 units Type IIs restriction enzymes (T7 DNA ligase, Esp31/BsaI-v2, NEB), and 1 μL T4 DNA ligase (NEB ...