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Citations for New England Biolabs :
151 - 200 of 324 citations for Acetylated Lysine Rabbit Polyclonal HRP labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Receptors were surface labeled by addition of Snap-Cell 647 SiR (1:1000, New England Biolabs) to the media for 20 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Ribosomes with a spytag labeled L17 subunit was used during in vitro translation system (NEB, E3313S) to generate the stalled RNC ...
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bioRxiv - Plant Biology 2023Quote: ... 4 pmol of double-stranded DNA was labeled with 1 unit of Klenow fragment polymerase (NEB) and 8 pmol Cy5-dCTP (Cytiva ...
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bioRxiv - Genetics 2023Quote: ... All probes were 5’ end-labeled with [alpha-32P]-ATP and T4 polynucleotide kinase (NEB Inc.). The gels were exposed to a PhosphorImager ...
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bioRxiv - Cell Biology 2023Quote: ... cells were labeled with 5 μM cell-impermeable SNAP-Surface Alexa Fluor 488 (New England Biolabs) in complete media (DMEM + 10% FBS ...
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bioRxiv - Genetics 2024Quote: ... All probes were 5’ end-labeled with [alpha-32P]-ATP and T4 polynucleotide kinase (NEB Inc.). The gels were exposed to a Phosphor Imager ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was probed with horseradish peroxidase (HRP)-conjugated anti-MBP antibody (NEB) and HRP-conjugated anti-FLAG antibody to detect MBP-TcpB and FBXO22 ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitation experiments were performed with 15 μL of Pan anti-glycine lysine antibody conjugated to agarose beads (PTM Biolabs, Chicago, IL, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Oligonucleotide probes were end-labeled with γ-32P ATP (Perkin-Elmer) and T4 PNK (New England Biolabs) per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The annealed product was labeled by fill-in synthesis using 5 U Klenow exo-(New England Biolabs) and a dNTP mix containing 40 µM each of dATP ...
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bioRxiv - Biophysics 2022Quote: ... The donor-labeled DNA was phosphorylated at the 5’ end using T4 Polynucleotide Kinase (New England BioLabs) for 1 hour at 37°C ...
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bioRxiv - Bioengineering 2019Quote: ... the cells were first labeled with cell-permeable substrate SNAP-Cell Fluorescein (New England Biolabs, Cat. # S9107S) overnight at 240 nM concentrations ...
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bioRxiv - Cell Biology 2021Quote: ... FHIP1B or FHIP2A-coated IgG beads were labeled with 5 µM HaloTag-Alexa-660 (New England Biolabs) in the column for 10 minutes at room temperature and unbound dye was removed with a 300 mL wash with TEV buffer at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... and tRNA-Gly-CCC (Table S1) were radioactively labeled with gamma-32P-ATP using T4 PNK (NEB). Pre-hybridization and hybridization were performed in OligoHyb Buffer (Applied Biosystems ...
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bioRxiv - Biophysics 2022Quote: Cells used for calcium imaging experiments were labeled using 1 µM SNAP-Surface Alexa Fluor 647 (NEB) and 4 µM Oregon Green 488 BAPTA-1 (Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... The labeled primer was purified by G-25 microspin column (Cytvia) and Monarch DNA purification kit (NEB). The successful conjugation of three digoxigenin moieties was confirmed by the shift of the molecular weight using denaturing urea polyacrylamide gel electrophoresis ...
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bioRxiv - Molecular Biology 2019Quote: FLAG-TEV-SNAP-Ago2 expressed in HEK293T cells was labeled with SNAP-Surface Alexa Fluor 647 (NEB) and immunopurified by an anti-FLAG antibody conjugated onto Dynabeads Protein G (Invitrogen) ...
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bioRxiv - Genomics 2019Quote: ... The nicked DNA was labeled with a fluorescent-dUTP nucleotide analog using Taq DNA Polymerase (NEB, #M0267) for one hr ...
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bioRxiv - Molecular Biology 2019Quote: ... 400 ng of eluted DNA fragments were end-labeled with T4 Polynucleotide Kinase (T4 PNK) (NEB M0201L) and 10 µCi of [α-32P]-ATP (Perkins Elmer NEG002A ...
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bioRxiv - Microbiology 2020Quote: ... suspended in 200 µL of phosphate saline buffer (PBS) and labeled with TMR-Star (New England Biolabs) for 30 min at 37°C in the dark at a final concentration of 250 nM ...
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bioRxiv - Molecular Biology 2021Quote: ... Table 4 was end labeled using gamma-ATP and T4 Polynucleotide Kinase radioactive labeling protocol from NEB. Labelled oligos were purified using GE Healthcare illustra ProbeQuant G-50 Micro Columns and the membrane was probed overnight ...
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bioRxiv - Molecular Biology 2022Quote: miRNAs were synthesized by IDT and radio-labeled at the 5’ end with T4 polynucleotide kinase (NEB) and (γ-32P ...
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bioRxiv - Microbiology 2022Quote: ... The glpFKD promoter fragments were labeled with 32P using DNA Polymerase I Large Fragment (Klenow fragment) (NEB) to fill in the 5’ overhang with [α-32P]dATP ...
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bioRxiv - Developmental Biology 2023Quote: ... Digoxigenin-labeled antisense Alk3 RNA probe was prepared by linearization with EcoR1 (New England Biolabs, Ipswich, MA) and transcription with T7 RNA polymerase (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... Receptors were surface labeled by addition of SNAP-Surface Alexa Fluor 546 (1:1000, New England Biolabs) to DMEM F12 without serum and phenol red (21041-025 ...
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bioRxiv - Molecular Biology 2023Quote: ... Digested DNA fragments were filled in with biotin-labeled dATP by incubating with Klenow enzyme (NEB, M0212), biotin-14-dATP ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was 5’-end-labeled with 32P using T4 polynucleotide kinase (New England Biolabs catalogue number M0201L). Samples were separated using 4–12% BisTris-PAGE and transferred to a nitrocellulose membrane (Protran) ...
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bioRxiv - Molecular Biology 2022Quote: ... rabbit anti-DYKDDDDK (#D6W5B; NEB) and rabbit anti-myc (#71D10 ...
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bioRxiv - Synthetic Biology 2022Quote: ... rabbit anti-SNAP-tag (NEB; P9310 ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... The membrane was incubated with HRP-conjugated (1/2,500 New England Biolabs, Ipswich, MA) or IRDye® (1/10,000 LI-COR Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNA was then 5’ end labeled in 24μL PNK wash buffer with 16μL of 1x PNK buffer (NEB) containing 150μCi of gamma P32-ATP ...
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bioRxiv - Molecular Biology 2019Quote: ... The 5’-monophosphate standard was generated by treating the 5’-triphosphate/3’-FAM-labeled 25mer with Apyrase (NEB). The 5’-hydroxyl standard was generated by treating the 5’-triphosphate/3’-FAM-labeled 25mer with calf intestinal phosphatase (NEB) ...
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bioRxiv - Biochemistry 2022Quote: 3’-PUA-DNA was generated by incubation of 1 μM 5’-FAM-labeled AP-DNA (FAM_U_35) with 20 U EndoIII/Nth (NEB) in Buffer B pH 7.0 at 37 °C for 60 m ...
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bioRxiv - Biophysics 2019Quote: ... Biotin- and digoxygenin-labeled handles were amplified with a non-proofreading Taq DNA polymerase (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Biophysics 2019Quote: ... Biotin- and digoxygenin-labeled handles were amplified with a non-proofreading Taq DNA polymerase (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Genomics 2019Quote: ... Nicked DNAs were labeled at 72°Cfor 60 minutes using 15 U Taq DNA Polymerase (New Engand Biolabs) in 1× each Labeling Buffer and Labeling Mix (Bionano Genomics) ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... was detected by addition of 1 nM Europium-labeled anti-p53 phosphoserine 15 antibody (New England Biolabs/ Cisbio) and 40 nM streptavidin-conjugated APC (Prozyme ...
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bioRxiv - Immunology 2020Quote: SNAP-ASC-CARD and CASP1-CARD-SNAP monomers that were labeled with Alexa Fluor 647 (New England Biolabs) were co-incubated with CARD domain only filament seeds (CARD8-CARD without SNAP ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA probes (Extended Table 1) at 500 nM were 5’-[32P]-labeled using T4 Polynucleotide Kinase (NEB) and hybridized to the membrane overnight at 37°C in PerfectHyb™ Plus Hybridization Buffer (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... this extension could be radioactively labeled by filling in the overhangs using 32P-dCTP and Klenow enzyme (NEB). Sequences of the +-strand oligonucleotides used are described in Table 1.
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bioRxiv - Cell Biology 2023Quote: ... Snap-tagged human β1AR and β2AR constructs were labeled with Snap-cell 647 SiR (New England Biolabs, S9102S) at 1:1000 concentration for 20 min at 37°C in DMEM without phenol red supplemented with 30 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... A stock solution of these oligos (1μl of a 10μM) was 5’-end-labeled using T4PNK (NEB®) and 5μl of ATP ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were hybridized with primary and HRP coupled secondary antibodies (New England Biolabs, Ipswich, MA). Membranes were revealed by chemiluminescence with SuperSignal West Dura or Femto reagents and data acquired using the G-BOX-iChemi Chemiluminescence Image Capture system (Syngene ...
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bioRxiv - Microbiology 2019Quote: ... rabbit α-Gaussia Luc (E80235, NEB), rabbit α-PB1 (PA5-34914 ...
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bioRxiv - Molecular Biology 2022Quote: ... and rabbit anti-myc (#71D10; NEB) primary antibodies were used ...
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bioRxiv - Molecular Biology 2021Quote: ... The immunoprecipitated RNA was 5’-end-labeled with 32P using T4 polynucleotide kinase (New England Biolabs catalogue number M0201L), separated using 4–12% BisTris-PAGE ...
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bioRxiv - Microbiology 2019Quote: ... 1 pmol of DNA template was mixed with 10 pmol of oligonucleotide 9 labeled with Yakima Yellow and 1µL of HemoKlen Taq DNA Polymerase (New England Biolabs) in a 6µL reaction mixture containing 50 mM Tris-HCl pH 9.0 ...
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bioRxiv - Biochemistry 2021Quote: ... S121A or triple mutant (AAA)) was incubated and labeled in vitro with 2U of CK2 (New England Biolabs, P60105) in a 30µl reaction containing 3pM adenosine triphosphate (ATP) ...
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bioRxiv - Biophysics 2021Quote: ... All proteins were purified and labeled with BG-surface Alexa 647 and BC-surface Dy547 dyes (New England Biolabs) as previously described (Murayama and Uhlmann ...
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bioRxiv - Microbiology 2022Quote: ... Oligonucleotides complimentary to the target were end-labeled with γ-32P-ATP by T4 polynucleotide kinase (New England Biolabs). Labeled oligonucleotides were added to the membrane and incubated overnight at 45°C ...