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Citations for New England Biolabs :
551 - 600 of 1353 citations for 7 Bromo 3 methyl 1H indole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... such that following digestion (3 units of BsrI (New England BioLabs, R0527) in NEB Buffer 3.1 (B7203) ...
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bioRxiv - Genetics 2020Quote: ... 3’-end RNA dephosphorylation was performed on beads with PNK (NEB M0201L) for 20 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Linker-2 (5′-GAGTCTGCGTGTGATTCGGGTTAGGTGTTGGGTTGGGCCA-3′) was ligated using T4 RNA Ligase1 (NEB). Reaction mixtures were resolved on a 10% TBE-Urea gel ...
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bioRxiv - Molecular Biology 2021Quote: ... and 3 µl Ultra II End-prep enzyme mix (New England Biolabs). Samples were incubated at 20°C for 5 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... 3’ adaptor was ligated to the cDNA using T4 ligase (NEB; M0202S). The cDNA was purified using Dynabeads myOne SILANE (life Technologies ...
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bioRxiv - Immunology 2021Quote: ... Step 3 was cloned into Step 4 with T4 DNA ligase (NEB) and then this recominbeering fragment (5’ arm of homology ...
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bioRxiv - Genomics 2021Quote: ... The 3’ ends were dephosphorylated with T4 polynucleotide kinase (T4 PNK; NEB) in the following reaction mix ...
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bioRxiv - Molecular Biology 2021Quote: ... Adenylated 3’ adapter was prepared using the 5’ DNA adenylation kit (NEB) and ligated using T4 RNA ligase 2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Adenylated 3’ adapter was prepared using the 5’ DNA adenylation kit (NEB) and ligated using T4 RNA ligas’ 2 ...
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bioRxiv - Genomics 2022Quote: ... 3′ Adapter ligation was done using T4 RNA Ligase 1 (NEB, M0204L). A first binding to streptavidin beads (NEB ...
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bioRxiv - Systems Biology 2023Quote: ... The second strand was synthesized using Klenow Fragment (3’ → 5’ exo-) (NEB). The dsDNA library was digested with Mlul-HF and Pad restriction enzymes (NEB ...
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bioRxiv - Microbiology 2022Quote: ... and 3 μL of Ultra-prep enzyme mix (New England Biolabs, UK), and completed with molecular biology grade water ...
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bioRxiv - Cell Biology 2022Quote: ... these cells were treated with 3 µM SNAP-Cell TMR-Star (NEB) for 30 min at 37°C and washed in 10% FBS DMEM for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... using Phusion High Fidelity polymerase with HF buffer and 3% DMSO (NEB) under the following cycling conditions ...
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bioRxiv - Cell Biology 2023Quote: ... and for A-tailing with Klenow Fragment (3’->5’ exo–) (NEB; M0212). A biotinylated primer (ENDseq-adaptor-1 ...
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bioRxiv - Biophysics 2023Quote: ... The sample was treated with 3 U/μL of micrococcal nuclease (NEB) in 50 mM HEPES-KOH pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM DTT) were treated with 3 ul (15 units) RNaseH1 (NEB) or H20 as a control and incubated at 37°C for 2 hr with slight agitation (300 rpm) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 3 µg were DNAsed with DNase I treatment (New England Biolabs). RNA was directly ribodepleted with the riboPOOL kit (siTOOLs ...
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bioRxiv - Genomics 2023Quote: ... 10 μL of Digestion-3 mix (5 U NotI-HF (NEB, R3189L) in 1X CutSmart buffer ...
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bioRxiv - Cell Biology 2024Quote: ... The 3′ cDNA adapter was ligated by T4 RNA ligase 1 (NEB) by incubating at 25 °C for 16h ...
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bioRxiv - Bioengineering 2023Quote: ... catalog #70015-3) using T4 DNA ligase (New England Biolabs, catalog #M0202L). We then packaged ligation reactions using T7Select415-1b cloning kit (Novagen (EMD Millipore) ...
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bioRxiv - Bioengineering 2024Quote: ... 2 and 3 were assembled by PCR assembly using Vent polymerase (NEB), with each reaction consisting of 1 x ThermoPol buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Adenylated 3’ adapter was prepared using the 5’ DNA adenylation kit (NEB) and ligated using T4 RNA ligase 2 ...
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bioRxiv - Cell Biology 2023Quote: ... Digested DNA was treated with Klenow Fragment (3’→5’ exo-) (NEB, M0212) in a final volume of 250 µL of 1x NEBNext dA-tailing reaction buffer (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... 3 μM CaCl2) and digested with 1/100 MNase (New England Biolabs). After addition of 250 µL sonication buffer (90 mM Hepes pH 7.9 ...
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bioRxiv - Plant Biology 2024Quote: ... ble cassette and 1 kb 3’ homologous arm onto the BamHI (NEB) digested backbone of pUC57 ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3 μl NEBNext Ultra II End Prep Enzyme Mix (NEB, E7546), incubated for 10 min at room temperature followed by 10 min at 65 °C ...
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bioRxiv - Genomics 2021Quote: ... and 7 PCR cycles were used to amplified libraries using NEBnext High-Fidelity 2X PCR Master Mix (New England Biolabs, Ipswich MA, Catalog #M0541S) and SYBR Green I (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: 3′ linker ligation (1x PNK buffer, 20 U T4 RNA ligase I (NEB), 20 U T4 RNA Ligase II truncated K227R ...
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bioRxiv - Cell Biology 2020Quote: ... the old H4-SNAP pool was first quenched with 3 μM BTP (NEB) for 30 min in complete medium ...
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bioRxiv - Genomics 2020Quote: ... and 3 μL of Uracil-Specific Excision Reagent (USER) Enzyme (NEB, Beijing, China) was then used with the size-selected and adaptor-ligated cDNA at 37°C for 15 min ...
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bioRxiv - Systems Biology 2022Quote: ... total RNA was first ligated to adaptors at the 3’ end by NEB 3’ SR adaptor and 5’ end by T4 RNA ligase followed by reverse transcription into cDNA using M-MuLV Reverse Transcriptase ...
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bioRxiv - Immunology 2022Quote: ... plates were washed 3 times with PBS-T and TMB substrate (Denovo Biolabs) was added for development of color ...
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bioRxiv - Genomics 2020Quote: ... 4 μL of 3 U/μL NEB T4 DNA Polymerase (New England Biolabs), 1 μL of 5U/μL Klenow (New England Biolabs) ...
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bioRxiv - Genomics 2019Quote: ... The mixture was supplemented with 5 µL DNA polymerase (3 U/µL, NEB) and 6 µL Klenow (5 U/µL ...
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bioRxiv - Neuroscience 2019Quote: ... 1 µM NGFR121W-SNAP was coupled with 3 µM BG549 surface (NEB # S9112) for 1 hour at 37°C in calcium imaging (CIB ...
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bioRxiv - Genetics 2020Quote: ... 3 µl of dNTPs (2.5 mM each) and 5 U Klenow exo- (NEB) and incubating for 1 h at 30°C ...
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bioRxiv - Biochemistry 2021Quote: 100 μl extension reactions were performed with Klenow Fragment (3’→5’ exo-) (NEB) (Figures 2B ...
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bioRxiv - Biophysics 2021Quote: ... where GRN-3 was expressed in Escherichia coli SHuffle cells (New England Biolabs) while other GRNs were expressed in Origami 2 DE3 cells (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The reaction was supplemented with 3 μl of T4 RNA ligase I (NEB), 1.5 μl of 10X reaction buffer ...
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bioRxiv - Biophysics 2021Quote: ... and poly(A)-tailed at their 3’ ends with poly(A) polymerase (NEB), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... We ligated a 3’ adapter ligation using T4 RNA Ligase 1 (NEB, M0204L). We performed a second bead binding followed by a 5’ decapping with RppH (NEB ...
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bioRxiv - Genomics 2022Quote: ... then perform A-tailing with Klenow Fragment (3’-> 5’ exo-) (NEB, Cat. M0212S) provided with dATP ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were preserved in a 1:3 glacial acetic acid: methanol (Biolabs-chemicals) solution and karyotyped using g-banding.
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bioRxiv - Molecular Biology 2020Quote: ... RNA was removed 3’phosphoryl groups using T4 Polynucleotide Kinase (New England Biolabs) in the buffer (70 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 ng of DNA was used per qPCR reaction (qPCR Master Mix, NEB). PCR was performed using an Applied Biosystems 7500 instrument (software version 2.3) ...
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bioRxiv - Genomics 2019Quote: ... and 3 µl of NEBNext Ultra II End Prep Enzyme Mix (E7545L, NEB) were mixed gently in 1.5 ml Eppendorf tube ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’ phosphorylation and 3’ dephosphorylation were performed with T4 PNK (NEB, Cat: M0201S) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and A-tailed using Klenow 3’-5’exo-enzyme (NEB, Cat. No. M0212). Illumina sequencing library adapters were subsequently ligated to DNA ends ...
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Glucocorticoid receptor collaborates with pioneer factors and AP-1 to execute genome-wide regulationbioRxiv - Genomics 2021Quote: ... In-line barcoded 3′ adapters were ligated with T4 RNA ligase I (NEB) for 12 hours at 20°C ...