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Citations for New England Biolabs :
101 - 131 of 131 citations for 7 Azabicyclo 2.2.1 heptane 7 ethanol 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The radioactive probes were prepared by end labelling 10 pmoles of DNA oligonucleotide complementary to the sfRNA (Supplementary table 7) with [γ-32P]-ATP (Perkin-Elmer, USA) using T4 polynucleotide kinase (NEB, USA). Unincorporated nucleotides were then removed by gel filtration on Illustra MicroSpin G-25 Columns (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... The PCR enrichment of adaptor-ligated DNA was conducted with 7 cycles and NEBNext® Multiplex Oligos for Illumnina® (NEB, USA) for single end barcoding ...
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bioRxiv - Microbiology 2020Quote: ... as previously described[7] Phage DNA libraries were prepared using NEBNext Ultra II FS library Prep and Kit for Illumina (New England Biolabs, Ipswich, USA). The sequencing was performed on the Illumina iSeq platform as part of a flowcell (2 x 151 ...
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bioRxiv - Genomics 2021Quote: ... 1000 ng samples of high quality total RNA (RIN≥ 7) was used for mRNA isolation using NEBNext Ploy(A) mRNA Magnetic Isolation module (New England Biolabs, catalog # E7490), followed by RNA library construction with NEBNext Ultra II Directional Lib Prep (New England Biolabs ...
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bioRxiv - Genomics 2020Quote: ... and once the solution is clear 48 uL of the elution was transferred to a 2mL Lo-Bind tube with End-Repair and A-tail buffer (7 uL) and enzymes (3 uL) premixed (NEBNext® Ultra™ II kit, New England Biolabs). The solution was then mixed by gentle tapping and transferred to a 0.2 mL PCR tube and incubated at 20 degrees C for 30 minutes and 65 degrees C for 30 minutes in a thermocycler ...
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bioRxiv - Immunology 2023Quote: ... PCR was performed with 2 ng of library input per reaction and 7 cycles of amplification using Q5 DNA polymerase (New England Biolabs, catalog # M0491S). Libraries were quantified using a Qubit 4 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA was isolated from 50ng of total RNA (RIN value >7) using the NEBNext® Poly(A) mRNA magnetic isolation module, (New England BioLabs, Hitichin, Herts, UK) (NEB, #E7490) and the sequencing libraries were prepared using the NEB® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared from 7 ng of RNA per sample using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB, E6420). Libraries were pooled and sequenced using a P3-100 kit from Illumina in a NextSeq2000 sequencer following manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... allowing a minimum RNA integrity number (RIN) of 7 (Schroeder et al., 2006) before fragmentation using NEBNext® Magnesium RNA Fragmentation Module (NEB, Ipswich, MA) into short fragments using divalent cations under high temperature ...
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bioRxiv - Synthetic Biology 2022Quote: In vitro transcription/translation by codon skipping of the short FLAG tag-containing peptides X-Val-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (XV-Flag) where X = 7, 13, 14, or 15 was carried out using the PURExpress® Δ (aa, tRNA) Kit (New England Biolabs, E6840S) based on a previous protocol with slight modifications 16 ...
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bioRxiv - Physiology 2024Quote: ... Total RNA was extracted from 30-35 sets of MTs isolated from 5-7-day old female flies in five independent biological replicates using the Monarch Total RNA Miniprep kit (New England BioLabs, Whitby, ON, Canada). Total RNA was quantified using a UV spectrophotometer (Synergy 2 microplate reader ...
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bioRxiv - Genomics 2021Quote: ... and 7 PCR cycles were used to amplified libraries using NEBnext High-Fidelity 2X PCR Master Mix (New England Biolabs, Ipswich MA, Catalog #M0541S) and SYBR Green I (ThermoFisher ...
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bioRxiv - Genomics 2023Quote: ... nuclei from 2 million MCF-7/NA12878 cells at a viability of ∼95% were resuspended in 200 μl of reaction buffer (1× NEB CutSmart buffer, 0.3 M sucrose). Nuclei were then treated with EcoGII by adding 200 U of EcoGII (NEB ...
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bioRxiv - Biophysics 2023Quote: ... precipitated with ethanol and digested overnight with BsaI (NEB # R3733S). Digested biotinylated arms were purified by spin column chromatography using MicroSpin S-400 HR (Amersham # 27514001).
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bioRxiv - Biochemistry 2020Quote: ... first by chloroform extraction and ethanol precipitation followed by Monarch RNA cleanup kit (NEB).
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bioRxiv - Plant Biology 2024Quote: ... The RNA was ethanol precipitated and phosphorylated with T4 polynucleotide kinase (New England Biolabs) prior to library construction using the NEXTFLEX Small RNA-Seq Kit v4 (Perkin Elmer) ...
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bioRxiv - Cell Biology 2022Quote: ... The RNA/ethanol mixture was transferred to a miniprep kit RNA purification column (NEB, T2010S), and RNA was isolated according to the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... The ethanol precipitated DNA constructs were digested with either HpaII or HhaI (New England BioLabs), which are methylation sensitive restriction enzymes ...
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bioRxiv - Microbiology 2023Quote: ... the pellet was washed with 80% ethanol and resuspended in 12 μl nuclease free water (NEB). 1 μl of the prepared cDNA library was subjected to Bioanalyzer analysis.
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bioRxiv - Biophysics 2022Quote: ... DNA was recovered by electroelution and ethanol precipitation and subsequently was treated with Nt.BbvCI (New England Biolabs) to introduce two nicks on the same stand near the middle of the molecule ...
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bioRxiv - Molecular Biology 2023Quote: ... Precipitated DNA was washed with 70% ethanol and resuspended in 1X NEBuffer #2 (B7002S, New England Biolabs) with 2 μl RNase A (20 mg/ml ...
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... After phenol/chloroform extraction and ethanol precipitation the samples were digested overnight with the secondary enzyme DpnII (NEB) followed by phenol/chloroform extraction ...
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bioRxiv - Synthetic Biology 2023Quote: ... The purified flexizyme was desalted using a Monarch RNA purification kit using 2X volume ethanol (New England Biolabs), concentration determined using a Nanodrop ND-1000 ...
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bioRxiv - Microbiology 2022Quote: ... RNAs were precipitated in cold ethanol and 0.3 M of sodium acetate and dephosphorylated using Calf-intestinal alkaline phosphatase (New England Biolabs), according to manufacturer protocol ...
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bioRxiv - Bioengineering 2022Quote: ... RNA was isolated by precipitation in isopropanol-ethanol 75% and resuspended in 10 μL nuclease free water (NEB, B1500) before quantification by BioDrop μLITE (Biodrop) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was eluted from the gel overnight in 400 µl of 0.4 M NaCl with rotation at RT and precipitated with 96% ethanol in the presence of linear acrylamide (NEB).
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bioRxiv - Genetics 2021Quote: ... The digest products were then ethanol precipitated and self-ligated at room-temperature overnight using T4 DNA Ligase (New England Biolabs). The resulting PCR products were restriction-digested with EagI (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... DNA was purified using standard Phenol:chloroform:isoamyl alcohol extraction and ethanol precipitation and used for library preparation with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) with 9 (H3K4me1 ...
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bioRxiv - Plant Biology 2021Quote: ... Forty-five micrograms of Erythronium and five micrograms of Potentilla gDNAs (concentrated by ethanol precipitation to 0.5 μg/μl) were digested with ApoI-HF enzyme (New England Biolabs, Hertfordshire, UK), loaded onto 1% agarose gel ...
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bioRxiv - Synthetic Biology 2024Quote: ... Purification of PCR products for downstream applications was accomplished using either phenol/chloroform ethanol precipitation or the Monarch® PCR & DNA Cleanup Kit (New England Biolabs) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Cleaved and purified DNA was purified by PCI and ethanol precipitation and was subjected to dA-Tailing using Klenow Fragment (NEB, no. E6053). Reactions were incubated for 30 min at 37 °C and immediately subjected to adapter ligation as per the manual of Oxford Nanopore Ligation-based library prep kit (ONT ...