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Citations for New England Biolabs :
1 - 50 of 682 citations for 6H BENZO CD PYREN 6 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... digested for 6h by SspI (20 U) (NEB), loaded on a 1% agarose gel (15×20 cm ...
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bioRxiv - Genomics 2021Quote: ... and 6h hours at 37°C with 20 U of MspI (New England Biolabs) in 30 μl of 1x NEBuffer 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... zHmgn2 CDS-FLAG and oHmgn2 CDS-FLAG were cloned into a pCS2 vector using NEBuilder HiFi DNA Assembly (New England Biolabs). The mRNAs for these constructs were synthesized from linearized plasmids using the mMessage mMachine SP6 Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... the pUbi63E-KDM5-HA plasmid was generated by replacing the Flag-Ubiquitin CDS sequence with the KDM5-HA CDS sequence using Gibson assembly technology provided by NEB (# E5510S).
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bioRxiv - Plant Biology 2021Quote: ... pTNT-CDS vectors were generated through Gibson assembly (NEB).
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bioRxiv - Genomics 2021Quote: ... 100 ng of genomic DNA were digested for 6h at 65°C with 20 U TaqI (New England Biolabs) and 6h hours at 37°C with 20 U of MspI (New England Biolabs ...
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bioRxiv - Developmental Biology 2023Quote: ... H2B CDS was obtained from pSNAPf-H2B (New England Biolabs). CDS of Ol-RanT27N and miRFP670-Nano3 was synthesized by eurofin ...
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bioRxiv - Genetics 2019Quote: Total genomic DNA (4 μg) of cells collected at each time point was digested for 6h with EcoRV (40 U) (NEB) loaded on a 1% agarose gel (15×20 cm ...
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bioRxiv - Plant Biology 2021Quote: ... and MRE11 CDS product were then merged using NotI and NdeI (NEB) restriction cloning to form the MR cassette (Supplementary Figures 13b and 12b) ...
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bioRxiv - Synthetic Biology 2021Quote: The recombinant CBX8-CD was expressed in BL21 (DE3) (New England Biolabs) Escherichia coli cells ...
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bioRxiv - Plant Biology 2022Quote: ... CDS of ATG6 was cloned into the pMal-C2X vector (New England Biolabs) for MBP fusion using infusion cloning (Takara Korea) ...
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bioRxiv - Microbiology 2019Quote: ... NEB One Taq One-Step RT-PCR Kit (NEB # E5310S) was used for nucleic acid amplification ...
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bioRxiv - Biochemistry 2021Quote: ... The AB and CD fragments were ligated with T4 DNA ligase (New England Biolabs) (1:1 molar ratio ...
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bioRxiv - Biochemistry 2021Quote: ... The AB and CD fragments were ligated with T4 DNA ligase (New England Biolabs) (1:1 molar ratio ...
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bioRxiv - Genomics 2021Quote: ... One μL APOBEC3A (NEB #E7120S) was added directly to the reaction with 10 μL of 10x APOBEC3A reaction buffer and 1 μL BSA (10 mg/mL) ...
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bioRxiv - Cell Biology 2023Quote: ... and one in-solution (NEB) DNase digestions were performed ...
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bioRxiv - Neuroscience 2021Quote: ... Genes of interest were amplified with the One Taq One-Step RT-PCR kit (NEB) using 1 μg of RNA ...
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bioRxiv - Microbiology 2021Quote: ... with Luna Universal One-Step Rt-qPCR & Probe One-Step RT-qPCR Kits (NEB, USA), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... One-step qRT-PCR was performed using the Luna Universal One-Step RT-qPCR Kit (NEB) and 5 ng RNA per reaction in technical duplicate with a StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Genetics 2020Quote: ... One was to use a kit to generate sequencing libraries in one-tube reactions (NEB, E7103S). Another modification was to spike-in the panel of synthetic nucleosomes carrying major histone methylations (EpiCypher ...
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bioRxiv - Genomics 2023Quote: ... one female and one male HRFI) were artificially methylated using the M.Sss1 Enzyme (New England BioLabs) following the manufacturer instruction ...
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bioRxiv - Microbiology 2021Quote: ... One microliter of RNase H (NEB) was added to each tube ...
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bioRxiv - Cell Biology 2023Quote: ... and one in-solution DNase (NEB) digestions were performed ...
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bioRxiv - Genetics 2023Quote: ... The T7 promoter and mtdTomato CDS were amplified from pmrPTRE-AAV using PTRE_floxed_F/R and Phusion High-Fidelity PCR Master Mix (NEB). NotI and SalI sites added by the primers were used to subclone this amplicon into pRM1506_TMM432 ...
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bioRxiv - Microbiology 2019Quote: ... lectin (70 to 1599-bp CDS of ACA1_164810) without their signal sequences into pMAL-p2x vector (New England Biolabs) for periplasmic expression in BL21-CodonPlus(DE3)-RIPL (Agilent Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... was cloned into phCMV3 vector by assembling two CDS fragments amplified from phCMV3-CatSpertS:WT (phCMV3-CatSpertS:Mut) using NEBuilder® HiFi DNA Assembly Kit (NEB). ORF sequences of CatSperτS:WT (phCMV3-CatSpertS:WT ...
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bioRxiv - Synthetic Biology 2022Quote: ... An Esp3I restriction site was introduced immediately upstream of the CHIPΔTPR CDS and GSGSG linker via the KLD Enzyme Mix (NEB) following PCR amplification with mutagenic primers (Genewiz) ...
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bioRxiv - Molecular Biology 2023Quote: ... Deletion variants of ATI2 were amplified from ATI2 (AT4G00355.1) CDS by overlap extension PCR using Phusion High Fidelity DNA polymerase (NEB) and subcloned into pCR™4/TOPO™ vectors (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: ... An Esp3I restriction site was introduced immediately upstream of the CHIPΔTPR CDS and flexible GSGSG linker via the KLD Enzyme Mix (NEB) following PCR amplification with mutagenic primers (Genewiz) ...
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bioRxiv - Neuroscience 2022Quote: ... One-step RT-qPCR was performed using the Luna universal One-Step RT-qPCR kit (New England BioLabs) in a total volume of 20 µl and a template concentration of 50 ng/µl according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2023Quote: ... NEB One Taq RT-PCR kit (One Taq® RT-PCR Kit, New England Biolabs INC, Frankfurt, Germany) was used for cDNA synthesis ...
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bioRxiv - Neuroscience 2024Quote: One-step RT-qPCR was performed using the Luna universal One-Step RT-qPCR kit (New England Biolabs) in a total volume of 10 µl and a template concentration of 50 ng/µl according to manufacturer’s recommendation ...
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bioRxiv - Genomics 2021Quote: ... One microliter of QuickCIP (New England Biolabs) was added and the solution was incubated at 37 °C for 10 min ...
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bioRxiv - Evolutionary Biology 2021Quote: ... One microliter of T7 endonuclease Ⅰ (NEB) was added to the sample ...
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bioRxiv - Bioengineering 2022Quote: ... and one with BseYI (NEB cat# R0635S) according to manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2024Quote: ... 6 and 4 μL of 6× Gel Loading Dye (B7025S, New England Biolabs) and 1.5 and 1 μL of 2.5 mg/mL EtBr were added ...
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bioRxiv - Plant Biology 2019Quote: The promoter region and full-length CKX2I or coding DNA sequence (CDS) were amplified by PCR (Table S2) from genomic DNA or cDNA using Q5 High-Fidelity DNA Polymerase (NEB) and cloned either alone or under of the 35S promoter together with GFP and mScarlet-i into pPLV03 or pGEX5x3 using Gibson Assembly (NEB) ...
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bioRxiv - Biochemistry 2021Quote: ... 700 bp upstream and downstream were amplified using the A–B and C–CD primer pairs from Table 2 (Phusion polymerase, GC buffer, New England Biolabs). The 2×myc tag was added to the B primers as overhangs ...
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bioRxiv - Microbiology 2021Quote: ... Restriction enzyme-cut pTOX3 was incubated with purified AB and CD PCR products along with a half-reaction of HiFi DNA Assembly Master Mix (NEB) according to manufacturer’s directions ...
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bioRxiv - Bioengineering 2020Quote: ... Respective peptide DNA coding sequences (CDS) were amplified from hACE2 via PCR and inserted using HiFi DNA Assembly Master Mix (NEB) for Gibson Assembly into the pcDNA3-SARS-CoV-2-S-RBD-Fc backbone linearized by digestion with NheI and BamHI ...
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bioRxiv - Microbiology 2020Quote: ... A plasmid containing Ptet-sigB was generated by cloning the 630Δerm sigB CDS amplified with oWKS-1498 and oWKS-1499 in pMiniT (NEB E1202) per manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... CDS plus 7–9 bp of untranslated sequence were amplified using High Fidelity Phusion Taq (New England BioLabs, NEB, USA), 3% DMSO vol/vol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... CDS plus 7–9 bp of untranslated sequence were amplified using High Fidelity Phusion Taq (New England BioLabs, NEB, USA), 3% DMSO vol/vol ...
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bioRxiv - Cell Biology 2021Quote: ... Gene specific primers with restriction sites added to the 5’ end were used to amplify the full CDS or gene fragments using Q5 DNA Polymerase (NEB). Primer sequences and restriction sites used for each construct can be found in Table S8 ...
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bioRxiv - Cell Biology 2020Quote: ... pCS2-luciferase CDS or pCS2-luciferase with G3BP1 5’UTR and 3’UTR were linearized with Sal I (New England Biolabs) and gel purified (Qiagen) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cRFXa CDS was amplified from cDNA using primers MC252 and MC253 and Q5 DNA polymerase (NEB Cat. No. M0491L), with 2 µl of cDNA template in a 50 µl PCR reaction and 35x cycles ...
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Ferroportin Depletes Iron Needed for Cell Cycle Progression in Head and Neck Squamous Cell CarcinomabioRxiv - Cancer Biology 2022Quote: ... The pLVX-TetOne-puro vector was digested with BamHI and EcoRI and combined with the FPN CDS using NEB HiFi DNA assembly master mix (New England Biolabs) to create the pLVX-tet-FPN vector ...
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bioRxiv - Neuroscience 2023Quote: The EEF1A2 CDS was cloned into pcDNA3.1-FLAG (gift from Saunders Lab) using NEBuilder® HiFi DNA Assembly Cloning Kit (NEB) according to the protocol of the manufacturer ...
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bioRxiv - Genetics 2022Quote: SLITRK3 constructs were cDNA synthesised by Genescript (USA) into pCMV-3Tag-4A by replacing the CDs for these inserts via using restriction enzymes BamHI and XhoI (NEB), giving rise to all SLITRK3 plasmid variants ...
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bioRxiv - Synthetic Biology 2023Quote: ... was replaced with a Renilla luciferase CDS via Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) to generate a dual-luciferase reporter containing luc2P and Renilla expression cassettes (pGL4.33R) ...