Labshake search
Citations for New England Biolabs :
551 - 600 of 5241 citations for 5 OXO 5 6 7 8 TETRAHYDRO NAPHTHALENE 1 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2022Quote: ... 5 U/μl T7 RNA polymerase (New England Biolabs), 1 U/μl SUPERase In (Thermo Fisher Scientific) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... and 5 U/µl T7 RNA polymerase (NEB M0251) in 1x RNAPol buffer (NEB) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 5 μM Mth RNA Ligase (New England Biolabs) was split into two 50 μl aliquots in thin-walled PCR tubes and incubated at 65°C in a thermal cycler with a heated lid set to 105°C for 1 hour ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 5 U/μl Protoscript II reverse transcriptase (NEB, M0368X), 1x Protoscript II reverse transcriptase buffer ...
-
bioRxiv - Plant Biology 2023Quote: ... 5 µl of 2X Luna qPCR Master Mix (NEB), 0.02 mM of forward and reverse primer ...
-
bioRxiv - Biophysics 2023Quote: ... myosin-5 was incubated with apyrase (New England Biolabs) at 25 units / mL working concentration at room temperature for 10 min before being added to the well ...
-
bioRxiv - Bioengineering 2023Quote: ... coli strains: NEB 5-alpha (NEB, C2987; not authenticated), BL21-AI (Thermo Fisher ...
-
bioRxiv - Cancer Biology 2023Quote: ... and transformation (chemically competent NEB 5- alpha E. coli) were performed according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... 3 μL 5× Phusion HF buffer (New England Biolabs), 2.7 μL dH2O ...
-
bioRxiv - Molecular Biology 2023Quote: ... followed by 5’ phosphorylation with T4 Polynucleotide Kinase (NEB) and self-ligation with T4 DNA Ligase (NEB).
-
bioRxiv - Genomics 2023Quote: ... +28 μl M.EcoGII at 5 U/μl (NEB M0603S). The DNA was purified using Qiagen PCR columns and quantified by measuring A260 ...
-
bioRxiv - Cancer Biology 2023Quote: ... NU-1611-Cy3),1.5ul of Klenow Fragment (3’→5′ exo-, NEB, M0212S) and 1.5ul of T4 Polynucleotide Kinase (NEB ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmids were transformed into NEB 5-alpha F’Iq (NEB). Standard chemically competent Escherichia coli transformation protocols were used to construct plasmid host strains ...
-
bioRxiv - Molecular Biology 2023Quote: ... followed by addition of 5 units Antarctic Phosphatase (NEB) and 2.5 µL 10 × Antarctic Phosphatase buffer ...
-
bioRxiv - Synthetic Biology 2024Quote: ... After 5 PCR cycles using Q5 polymerase (NEB #M0491) with 5X GC Buffer with a melting temperature of 72°C ...
-
bioRxiv - Developmental Biology 2024Quote: ... 5 U BsmBI (New England Biolabs, Cat. No. R0580), 3 U BglII (New England Biolabs ...
-
bioRxiv - Microbiology 2024Quote: ... and 5% v/v PEG 8000 (New England Biolabs) during ligation.
-
bioRxiv - Biophysics 2024Quote: ... and Monarch PCR&DNA Cleanup Kit (5 μg) (NEB). The purified dsDNA templates were transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB ...
-
bioRxiv - Microbiology 2024Quote: ... then incubated with 5 units of Antarctic phosphatase (NEB) at 37°C overnight ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1.5ul of Klenow Fragment (3’→5′ exo-, NEB, M0212S) and 1.5ul of T4 Polynucleotide Kinase (NEB ...
-
bioRxiv - Molecular Biology 2020Quote: ... using 8 units of T4 RNA ligase 1 (NEB) and 8 nmol of ATP in a final volume of 8 μl for 1 h at 37°C ...
-
bioRxiv - Bioengineering 2022Quote: ... 1X of a restriction enzyme mix [1/2 EcoRI-HF® (NEB #R3101, 2/5 Nuclease-free water, 1/10 CutSmart Buffer 10X (NEB)] ...
-
bioRxiv - Immunology 2024Quote: ... Insert and vector were combined at a 5:1 ratio and held at 50 °C for 1 hour in NEBuilder® HiFi DNA Assembly Master Mix (NEB). Gibson assembly products were purified using the MinElute® PCR Purification Kit (Qiagen) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 8 μL of solution A and 6 μL of solution B were mixed with 16 U RNase Inhibitor (NEB), gBlock templates (2 nM each ...
-
bioRxiv - Neuroscience 2020Quote: ... with TdTomato was amplified by PCR with primers (5’- ggcgcgCCCCCCTCTCCCTCCCCCCC -3’ and 5’- ggcgcgccTTACTTGTACAGCTCGTCCATGCCGTACAG -3’) using Hot start Q5® polymerase (NEB, Frankfurt, Germany) from LeGO-iT (a gift from Boris Fehse ...
-
bioRxiv - Molecular Biology 2021Quote: ... 8 μg of digested nucleic acids were treated or not with 10 μl of RNase H (New England BioLabs, M029L) overnight at 37 °C in 1x RNAse H buffer and 1/10 of the samples were used as input ...
-
bioRxiv - Genomics 2021Quote: ... the purified products are treated with Klenow fragment (3’ → 5’ exo-) (Cat. No. M0212L; NEB; use 1 uL) and Taq DNA polymerase (Cat ...
-
bioRxiv - Microbiology 2021Quote: ... the cap-1 structure was added to the 5’ end using the Vaccinia capping enzyme (New England Biolabs) and Vaccinia 2’-O-methyltransferase (New England Biolabs) ...
-
bioRxiv - Biochemistry 2022Quote: 3’-PUA-DNA was generated by incubation of 1 μM 5’-FAM-labeled AP-DNA (FAM_U_35) with 20 U EndoIII/Nth (NEB) in Buffer B pH 7.0 at 37 °C for 60 m ...
-
bioRxiv - Cancer Biology 2019Quote: ... and then resuspended in sorting buffer (PBS with 0.5% BSA, 2.5 mM MgCl2, 0.5 mM CaCl2, 1 µg/mL DAPI and 100 U/mL DNaseI, NEB) and incubated for 30 minutes at room temperature before sorting ...
-
bioRxiv - Biophysics 2021Quote: ... The phosphorylated 5’-ends were subjected to RNA ligation by 20 units of T4 RNA ligase 1 (NEB) in the presence of 10 mM DNA/RNA rP5_RND oligo (Supplemental Table 3 ...
-
bioRxiv - Molecular Biology 2023Quote: 9N_VRA3 adapter oligonucleotide (Supplementary Table 1) was pre-adenylated with the 5’ DNA Adenylation Kit (New England Biolabs) according to the manufacturer’s protocol at a 5X scale ...
-
bioRxiv - Molecular Biology 2023Quote: ... The DNA probes (Extended Table 1) at 500 nM were 5’-[32P]-labeled using T4 Polynucleotide Kinase (NEB) and hybridized to the membrane overnight at 37°C in PerfectHyb™ Plus Hybridization Buffer (Sigma-Aldrich) ...
-
bioRxiv - Microbiology 2023Quote: In vitro transcribed CHIKV RNA (nt’s 1-337) was 5’ dephosphorylated using Quick CIP according to the manufacturer’s instructions (NEB) before purification using an RNA Clean & Concentrator column (Zymo Research) ...
-
bioRxiv - Molecular Biology 2023Quote: ... followed by on-bead 5’ ligation of a biotinylated REL5 linker sequence using T4 RNA ligase 1 (NEB) for 3 h at 37⁰C ...
-
bioRxiv - Microbiology 2024Quote: ... 5 μl of 10 mM MnCl2 and 1 μl (400 units) of λPP (New England Biolabs, Ipswich, MA). Untreated lysates received 1 μl of H2O in place of λPP ...
-
bioRxiv - Cell Biology 2020Quote: ... 5 µl 10x buffer for Protein MetalloPhosphatases (New England Biolabs), and 5 µl 10 mM MnCl2 for 30 min at 30 °C ...
-
bioRxiv - Molecular Biology 2021Quote: ... 5 μl 400 U/μl T4 DNA Ligase (NEB, M0202L) and 969 μl water ...
-
bioRxiv - Genomics 2022Quote: Library E was transformed into 5-alpha E.coli (NEB, C2987H), recovered in SOC medium (NEB ...
-
bioRxiv - Molecular Biology 2019Quote: ... with 5 units of calf intestinal alkaline phosphatase (CIP, NEB) for 30 min at 37°C ...
-
bioRxiv - Microbiology 2019Quote: ... coli strains NEB 5-alpha F’lq (New England Biolabs, US) and MC1061 with plasmid pKD46 which carries ARA-inducible Red recombination system24 ...
-
bioRxiv - Biochemistry 2020Quote: ... After a treatment with antarctic phosphatase (5 Units, NEB Inc.) for 30 minutes at 4°C ...
-
bioRxiv - Genomics 2021Quote: ... Adenylation was performed with 3’-5’ Klenow Fragment (NEB M0212L). Adaptors were ligated with NEB Quick Ligase for 10 minutes at 30°C before two rounds of cleanup with homemade beads ...
-
bioRxiv - Bioengineering 2021Quote: ... 5 μL of 5X Quick Ligation Reaction buffer (NEB: B6058S), 6 μL of the RMX adapter and 3 μL of T4 DNA ligase (2,000,000 units/mL) ...
-
bioRxiv - Genetics 2020Quote: ... 5 μl of Klenow exo- (NEB M0212S/L, 5U/μl) was added ...
-
bioRxiv - Genetics 2020Quote: ... and 5 μl of 400 U/μl T4 ligase (NEB). To maximize the ligation efficiency for the low input Tri-HiC protocol ...
-
bioRxiv - Genomics 2021Quote: ... and 4 μl 5 U/μL I-SceI (NEB, #R0694L) in a 50 μL final volume for 3 hours at 37°C ...
-
bioRxiv - Plant Biology 2021Quote: ... using a Klenow fragment (3′ → 5′ exo−) (New England Biolabs). Hybridization was performed at 65°C overnight in the pre-hybridization solution containing 6x saline-sodium citrate buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... 125 to 75 U/ 5 μg RNase B (Cat.P7817S, NEB) and fetuin (Cat ...
-
bioRxiv - Plant Biology 2020Quote: ... 5 μl of 2X RNA loading dye (New England Biolabs) was added ...