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Citations for New England Biolabs :
1 - 50 of 4567 citations for 3 1 Pyrrolidinylmethyl phenyl magnesium bromide solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... 3 μL 1:1,249 diluted Fe2+ solution (NEB)) at 37°C for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... Amplified PCR products were ran on 2% agarose gel and stained with ethidium bromide solution (New England Biolabs Inc.). Gel photographs were taken using ChemiDoc MP gel documentation system (BIO-RAD).
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bioRxiv - Bioengineering 2024Quote: ... 6 mM Magnesium Sulfate (MgSO4 – NEB #B1003), 1.4 mM deoxynucleotide (dNTP ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified ribodepleted RNA samples were fragmented for 3 minutes at 94°C in Magnesium RNA Fragmentation Module (New England Biolabs) and 3’ ends were dephosphorylated with T4 polynucleotide kinase (Lucigen) ...
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bioRxiv - Biochemistry 2020Quote: ... Purified embryo mRNA was fragmented to 100-200 nt RNA fragments by magnesium-buffer based degradation using the NEBNext Magnesium RNA Fragmentation Module (NEB, E6150S). Fragmentation was optimized for time and RNA input amount monitoring RNA size using the mRNA Pico Chip (Agilent ...
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bioRxiv - Plant Biology 2022Quote: ... and was fragmented using Magnesium RNA Fragmentation Module (NEB) at 86 °C for 7 min ...
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bioRxiv - Immunology 2022Quote: ... A first round of PCR was performed using 1× Standard-Taq magnesium free reaction buffer pack (NEB) with 2 mM MgCl2 (NEB) ...
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bioRxiv - Genomics 2020Quote: ... was fragmented using NEBNext Magnesium RNA fragmentation module (NEB, USA) for 1 minute at 94°C ...
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bioRxiv - Microbiology 2020Quote: ... 45 s using the NEBNext Magnesium RNA Fragmentation Module (NEB) followed by RNA purification with the Zymo RNA Clean & Concentrator kit ...
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bioRxiv - Synthetic Biology 2024Quote: ... 20 mM Tris-acetate, 10 mM magnesium acetate, 100 μg mL−1 BSA at pH 7.9; New England Biolabs). The reactions were incubated at 37 °C for 20-45 min ...
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bioRxiv - Bioengineering 2024Quote: ... GTP was added to a final concentration of 2 mM along with a buffer including magnesium (50 mM Tris-HCl, 10 mM MgCl2, 1 mM DTT, pH = 7.5; New England Biolabs) into circRNA solution ...
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bioRxiv - Molecular Biology 2024Quote: ... mRNA was fragmented using the NEBNext Magnesium RNA fragmentation module (NEB, E6150S) and purified using Monarch RNA Cleanup Kit (NEB ...
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bioRxiv - Genomics 2023Quote: ... This 17 µl of DNA solution was incubated with 3 µl digestion mixture containing 1 µl BciVI (New England Biolabs, cat. no. R0596S) and 2 µl CutSmart buffer (New England Biolabs ...
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bioRxiv - Immunology 2020Quote: ... After fragmentation of the RNA in a magnesium buffer (NEB Next RNA fragmentation buffer), the RNA fragments were purified using Agencourt RNAClean XP beads ...
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bioRxiv - Genomics 2022Quote: ... Extracted RNA was fragmented using the NEBNext Magnesium RNA Fragmentation module kit (NEB, USA), heating for 2min ...
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bioRxiv - Molecular Biology 2024Quote: ... mRNA samples were fragmented using the NEBNext® Magnesium RNA Fragmentation Module (NEB, E6150S) at 94°C for 4 minutes ...
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bioRxiv - Genetics 2020Quote: ... The PCR products were run on a 1% agarose gel stained with ethidium bromide alongside a 100 bp ladder (New England Biolabs, Ipswitch, MA, USA).
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bioRxiv - Molecular Biology 2022Quote: ... then RNA was fragmentated to ~150 nt by magnesium RNA fragmentation buffer (New England Biolabs) for 4 min at 94°C ...
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bioRxiv - Genomics 2023Quote: ... 1.5 μL 1:1,249 diluted Fe2+ solution (NEB), and 8.4 μL water ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μl dATP solution 100 mM (NEB; # N0440S), 1 μl BSA (20 mg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was PCR amplified (primers hFUR Exon 1-3 PCR For and hFUR Exon 1-3 PCR Rev) and purified (Monarch PCR & DNA cleanup kit, NEB). The resulting PCR product was sequenced using the hFur Exon 1 Seq primer.
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bioRxiv - Genomics 2024Quote: ... 2.67 uL of 10X RNA Fragmentation Buffer from the NEBNext Magnesium RNA Fragmentation Module (E6150S, NEB) was added ...
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bioRxiv - Genomics 2023Quote: ... 2.5 µl of 100 mM dATP solution and 2.5 µl of Klenow Fragment (3′->5′ exo-) (NEB, #M0212L) were added to the mixture ...
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bioRxiv - Genomics 2020Quote: Fragmentation of plasma RNA was done by using an NEBNext Magnesium RNA Fragmentation Module (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... we dilute the stock DNA to a final concentration of 6 mg/mL with 1/10th volume of 10× CutSmart Buffer (0.5 M Potassium Acetate, 0.2 M Tris-acetate, 0.1 M Magnesium Acetate; New England BioLabs), 0.1% Tween (to prevent non-specific interactions) ...
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bioRxiv - Plant Biology 2023Quote: ... 2µg of polyA enriched RNA was fragmented using NEBNext® Magnesium RNA Fragmentation Module (New England Biolabs) with incubation at 94°C for 1 min ...
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bioRxiv - Bioengineering 2024Quote: ... the poly (a) RNA was fragmented into small pieces using a Magnesium RNA Fragmentation Module (NEB, e6150) at 94℃ for 5-7 min ...
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bioRxiv - Immunology 2023Quote: ... 1μl Cas9 (diluted 1:3 in diluent B, NEB), 1μl water (water was replaced with 100ng/μl trib1 RNA for cop1 experiments) ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-cleaved caspase-3 1:100 (9661, NEB), rat anti-p21 1:100 (ab107099 ...
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bioRxiv - Genomics 2021Quote: ... 0.5 μL of 1 mg/mLbovine serum albumin solution (NEB), 1.2 μL of 50 mM NAD+ (NEB) ...
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bioRxiv - Genomics 2022Quote: ... 1% bovine serum albumin (BSA) solution (NEB, 20 mg/mL). Nuclei were pelleted at 600 RCF for 5 minutes at 4C ...
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bioRxiv - Neuroscience 2023Quote: ... Amplicons were then quantified on ethidium bromide stained agarose gels against a DNA marker (100 bp ladder, NEB), pooled and then Illumina sequenced (paired end ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were fragmented at 94°C for 2.75 min as per the NEBNext Magnesium RNA Fragmentation Module (NEB) with subsequent RNA purification using the Zymo RNA Clean & Concentrator kit ...
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bioRxiv - Microbiology 2024Quote: ... The cross-linked vRNA was digested with the NEBNext Magnesium RNA Fragmentation Module (New England Biolabs, Ipswich, MA) at 94°C for 2.5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Fragmentation of mRNA was performed using divalent cations under elevated temperature (Magnesium RNA Fragmentation Module (NEB, cat.e6150, USA) under 94℃ 5-7 min) ...
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bioRxiv - Genomics 2020Quote: ... 1 μl (3 U/μl) of T4 DNA polymerase (NEB), 1 μl (10 U/μl ...
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bioRxiv - Genetics 2023Quote: ... 1 μl T4 PNK (3′ phosphatase minus, New England BioLabs)) ...
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bioRxiv - Biochemistry 2022Quote: ... 1× CutSmart buffer (50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate,100 μg/mL BSA, pH 7.9, NEB), 20 U RiboLock ...
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bioRxiv - Genetics 2020Quote: The long fractions (with and without rRNA depletion) were further processed with the NEBNext Magnesium RNA Fragmentation Module (NEB), as described in the manual ...
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bioRxiv - Cancer Biology 2023Quote: ... the poly(A)+ RNA was sheared into 100–200-nt fragments using Magnesium RNA Frag mentation Module (NEB, E6150S). A portion of poly(A)+ RNA fragment was directly used as input ...
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bioRxiv - Synthetic Biology 2024Quote: ... 20 mM Tris-acetate, 10 mM magnesium acetate, 100 µg/mL recombinant albumen at pH 7.9; New England Biolabs) and 0.75 µL DpnI (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... Enriched poly(A) RNA was fragmented into small pieces using Magnesium RNA Fragmentation Module (New England Biolabs, cat.e6150, USA) under 86℃ for 7 m ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 1 μl of 10mM dATP and 3 μl of 5 U/μl Klenow fragment 3’ -> 5’ exo- (NEB M0212) in the thermocycler with the following program ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 nl of a solution containing 10µM EnGen Cas9 NLS (NEB) and 100 ng/µl of gRNAs was injected at the one-cell stage ...
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bioRxiv - Genomics 2021Quote: ... 2 μL of 1 mg/ml bovine serum albumin solution (NEB), 1 μL of hOGG1 (ProSpec TechnoGene Ltd. ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were fixed in 1:3 glacial acetic acid:methanol (Biolabs-chemicals) solution and the G-banding karyotype was determined ...
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bioRxiv - Molecular Biology 2024Quote: ... steps 1–3: GELase was replaced by β-agarase I (NEB). The reaction (1 U per plug ...
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bioRxiv - Molecular Biology 2020Quote: ... Ribodepleted RNA were then ligated to 10 pmol of a biotinylated 3’ adapter, (3’-Adap TAIL-seq, Supplementary Table 7) using 10 units of T4 RNA ligase 1 (NEB) in a final volume of 10 μl for one h at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... FP: 5’-AGCAAGGCTAGCCAAGACAAGTTTGTAC-3’ and RP: 5’-ACTCACGGGCCCTAGTGGGCAGATCTT-3’ and cloned between Nhe-1 and Apa1 (NEB, Ipswich, MA, USA) sites in mec4p::Lamp-1::GFP.
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bioRxiv - Microbiology 2023Quote: ... the gRNA cassette carrying the human U6 promoter and the invariant scaffold sgRNA sequence was inserted into the HIV-1 NL4-3 and HIV-1 CH077 pro-viral DNA between separated Nef and 3’LTR region using homologous recombination (NEB builder Hifi DNA assembly mastermix ...