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Citations for New England Biolabs :
451 - 500 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... TCRs were then amplified by PCR (20 cycles with the Phusion from NEB) with a single primer pair binding to the constant region and the adapter linked to the TRAV/TRBV primers added during the reverse transcription ...
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bioRxiv - Biochemistry 2021Quote: ... 500 µL supernatant was applied to 20 µL amylose resin (New England Biolabs) and incubated for two hours at 4°C ...
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bioRxiv - Genetics 2022Quote: ... Each reaction contained 20 μL and used Q5 polymerase (New England Biolabs, #M0491). The reagents were distributed into each well of a 96 well plate as follows ...
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bioRxiv - Microbiology 2019Quote: ... cholerae RNA at 37°C with 20 units of T4 RNA ligase (NEB) in a 20 µL reaction containing 1X T4 RNA ligase buffer ...
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bioRxiv - Microbiology 2019Quote: ... 20] and LongAmp™ Taq 2X Master Mix (New England Biolabs, Ipswich, MA). Amplification was performed using an Applied Biosystems Veriti™ Thermal Cycler (Thermo Fischer Scientific ...
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bioRxiv - Genomics 2019Quote: ... 2 µg of genomic DNA was digested with 20 units of MspI (NEB) overnight at 37C in a volume of 100 µl ...
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bioRxiv - Microbiology 2019Quote: ... 20 µl of 10x DNase buffer and 4 µl DNase I (NEB Inc.) were added and incubated at 37°C for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... Sticky ends were created by injecting 20 units of USER enzyme (NEB, M5505S) and incubating with 50 μL of 1X TA buffer (Teknova ...
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bioRxiv - Genomics 2021Quote: ... samples were incubated with 20 μl Oligo d(T) Magnetic Beads (NEB, S1419S) in PCR strips for selection of polyadenylated (poly(A) ...
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bioRxiv - Microbiology 2019Quote: ... and 5’ caps were removed by incubation with 20 U of RppH (NEB) for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were digested with 20 units of XmnI and XcmI respectively (NEB) to distinguish cleavable mutant from un-cleavable wild-type amplicons ...
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bioRxiv - Genetics 2020Quote: ... and a degenerate 20-mer (primer jklab0172) was inserted by HiFi Assembly (NEB). The resulting barcoded clone pools were transformed ...
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bioRxiv - Genomics 2020Quote: ... 10 µ of 20 mg/mL BSA (New England Biolabs, catalog no. B9000S), and 930 µ of nuclease-free water ...
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bioRxiv - Genomics 2020Quote: ... followed by addition of 20 μl Ligation master mix (containing: 15 μl NEB Quick ligation buffer ...
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bioRxiv - Biochemistry 2021Quote: ... 300 nM MS2-MBP was prebound to 20 μL of amylose resin (NEB) in EMB 300 Buffer ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was passed through a 20 ml amylose resin (New England BioLabs) and washed with 10 column volumes (CVs ...
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bioRxiv - Genomics 2020Quote: ... 15 mM HEPES pH 8) and 20 μl of DNase I (M0303S, NEB). The Nuclease Flushing mix was loaded into the flow cell and incubated for 30 min ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA (20 ng) was PCR amplified using LongAmpTaq 2X master mix (NEB). The PCR amplicons of selected regions were subjected to Sanger sequencing and BLAST analysis to confirm the presence of eight variants using the primers listed in Supplemental Table 1.
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bioRxiv - Immunology 2022Quote: ... ∼50 μg of protein were incubated with 20 units of λPP (NEB, USA) for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... after which the template was removed by treatment 20 U of DpnI (NEB), a methylation-sensitive restriction enzyme ...
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bioRxiv - Synthetic Biology 2022Quote: ... for assembly into levels 2 in 20 μl 1x T4 ligase buffer (NEB). The reaction condition was ...
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bioRxiv - Molecular Biology 2022Quote: 5’ end phosphorylation (1x PNK buffer, 20 U T4 PNK (NEB, Cat# M0201L), 80 U RNaseOUT ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.0125 μl Exonuclease I (20 U/ml; New England Biolabs, cat. no. M0293S) and 2.36 μl of water were added ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 µL supernatant was applied to 20 µL amylose resin (New England Biolabs) and incubated for two hours at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... 0.05% Tween-20) containing 10 µMnt of circular ssDNA (PhiX174 virion DNA, NEB) immobilised on magnetic Streptavidin-coated beads (Dynabeads M-280 ...
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bioRxiv - Bioengineering 2023Quote: ... and 20 units/mL of Vent® (exo-) DNA Polymerase (NEB, cat# M0257L) or alternatively with Taq (NEB ...
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bioRxiv - Immunology 2023Quote: ... 500ng of gDNA was digested with 20 units XbaI (New England Biolabs, Germany) for one h ...
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bioRxiv - Genomics 2023Quote: ... 2X hybridisation buffer (4X SSC, 20% dextran sulphate, 2 mg/mL BSA (NEB), 1/10 volume nuclease free water and 1/10 volume vanadyl-ribonucleoside complex (VRC ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by addition of 20 µl of 0.5 mg/ml RNase A (NEB) and incubation at 37°C for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μg genomic DNA was digested with 20 units Sty I (NEB, R3500S) at 37 °C for 3 h ...
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bioRxiv - Genomics 2024Quote: ... After adding 10 µL of 20 mg/µL proteinase K (New England Biolabs), proteins were degraded for 30 minutes at 55°C followed by an overnight incubation at 68°C with shaking to reverse crosslinks ...
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bioRxiv - Molecular Biology 2024Quote: ... with the addition of 20 U of Murine Rnase Inhibitor (New England Biolabs). qPCR reactions were run in 12 μl with PowerUpTM SYBRTM Green Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... Ligations were performed in 96 well-plates with 1U T4 Ligase (NEB) and incubated at room temperate overnight ...
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bioRxiv - Cell Biology 2023Quote: ... the plate libraries were pooled together and AmpureXP beads (New England BioLabs) were used to purify the cDNA ...
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bioRxiv - Microbiology 2021Quote: ... 8 μL of RNA was added per section to step 2.1 through to 2.11.11 of the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB) using NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg of RNA was used for cDNA synthesis using the ProtoScript® II kit (New England BioLabs). qPCR reactions were carried out with FastStart Essential DNA Green Master (Roche ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 μg of DNA library was used as a template for mRNA synthesis by HiScribe T7 kit (NEB). The product was purified by NH4Ac precipitation and dissolved in RNAse-free water to a final concentration of 3 μg/ul.
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bioRxiv - Developmental Biology 2022Quote: ... and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1, NEB, E7335L). Conditions for PCR used are as follows ...
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bioRxiv - Developmental Biology 2022Quote: ... and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1, NEB, E7335L). Conditions for PCR used are as follows ...
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bioRxiv - Molecular Biology 2023Quote: 9N_VRA3 adapter oligonucleotide (Supplementary Table 1) was pre-adenylated with the 5’ DNA Adenylation Kit (New England Biolabs) according to the manufacturer’s protocol at a 5X scale ...
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bioRxiv - Molecular Biology 2023Quote: CDKN1A intron 1 APA 3’ splice site was deleted using Q5 Site-Directed Mutagenesis Kit (New England Biolabs) as per the manufacturer’s instructions using forward primer (5’-TCCCCACCCCAAAATGACGCGCAGCC-3’ ...
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bioRxiv - Cell Biology 2020Quote: ... 800ng were treated for 20’ at 37°C with 50u ShortCut Rnase III (NEB), in the digestion buffer supplied by the manufacturer ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 ng of genomic DNA was subjected to MspI digestion (NEB, Beverly, MA, USA), with subsequent end repair/dA-tailing reaction using Klenow Fragment (3’-5’ exo- ...
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bioRxiv - Evolutionary Biology 2021Quote: ... This 20 μL 10-cycle PCR reaction was performed using Q5 polymerase (NEB M0491L) following the manufacturer’s guidelines ...
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bioRxiv - Genetics 2020Quote: ... A 500 ng aliquot of DNA was digested with 20 units of AflIII (NEB) overnight at 37°C and subjected to Southern blot analysis (1% agarose gel in 1xTAE ...
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bioRxiv - Systems Biology 2020Quote: ... reaction components were added for second strand synthesis (70 μL water, 20 μL NEB second strand buffer ...
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bioRxiv - Immunology 2020Quote: ... Samples (20 μL) were loaded in DNA loading buffer (5 μL; New England Biolabs) and gels were run at 60 V for 120 mins.
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bioRxiv - Genomics 2019Quote: ... then 20 µL of the end-repair mix [3.5X NEB ligation buffer (NEB B0202S), 17.5 mM dNTP mix ...
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bioRxiv - Genetics 2021Quote: ... Radioactive end-labeling was performed with the addition of T4 PNK (20 Units, NEB) and 2 μl [γ-32P]ATP (6000Ci/mmol ...
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bioRxiv - Genetics 2021Quote: ... heiBE4-Gam was assembled into pCS2+20 by NEBuilder® HiFi DNA Assembly (NEB) with four inserts using Q5 polymerase PCR products (NEB) ...