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Citations for New England Biolabs :
1 - 50 of 1891 citations for 2 p sec Butylphenyl propionic Acid d3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... coli competent cells (D3,NEB-C2527H) were transformed with a pSANG10-3F-BG4 plasmid (Addgene #55756) ...
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bioRxiv - Biochemistry 2024Quote: ... E.coli BL21 (D3) strains (New England Biolabs) were transformed with a T7-driven effector plasmid and an E.coli constitutive promoter J23119 to drive sgRNA expression ...
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bioRxiv - Cell Biology 2024Quote: ... was introduced into BL21 cells (D3,NEB-C2527H) by heat shock ...
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bioRxiv - Cell Biology 2021Quote: ... ∼600-bp homology arms for each target locus were amplified from N2 genomic DNA and then inserted into the GFP^SEC^3xFlag vector pDD282 or the TagRFP^SEC^3xMyc vector pDD286 via Gibson Assembly (New England BioLabs). Hygromycin was used to select the knock-in candidate lines based on the strategy described in Dickinson et al [37].
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bioRxiv - Cancer Biology 2024Quote: ... was PCR amplified (14 cycles: 10sec 98°C, 30 sec 57°C, 20 sec 72°C) with Phusion polymerase (New England Biolabs (NEB)) ...
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bioRxiv - Microbiology 2022Quote: ... The resulting nucleic acids were treated with 2 U DNase I (New England Biolabs), 10 U S1 nuclease (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... and 72°C for 30 sec) with Q5 polymerase (NEB Inc.) and appropriate primers (S2 Table).
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bioRxiv - Molecular Biology 2024Quote: ... and 72°C for 30 sec) with Q5 polymerase (NEB Inc.) and appropriate primers (Supp ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 sec 72°C) with Phusion polymerase (New England Biolabs (NEB)) ...
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bioRxiv - Microbiology 2022Quote: ... aliquots of 200 ng nucleic acid were treated with 2 U DNase I (New England Biolabs), 10 U S1 nuclease (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... To digest sialic acid: 2 μL of α2-3,6,8 Neuraminidase (50U/mL, New England Biolabs, NEB) with GlycoBuffer 1 (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... To digest sialic acid: 2 μL of α2-3,6,8 Neuraminidase (50U/mL, New England Biolabs, NEB) with GlycoBuffer 1 (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... and cloned into an SEC backbone plasmid (NEBuilder HiFi DNA Assembly Master Mix, NEB). The modified Cas9/sgRNA plasmid ...
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bioRxiv - Cell Biology 2021Quote: 500mM p-Nitrophenyl Phosphate (pNPP, NEB, P0757S) substrate solution was diluted to 20 mM in phosphatase reaction buffer (PRB ...
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bioRxiv - Bioengineering 2024Quote: ... M13mp18 circular DNA (P- 107, Bayou Biolabs) was used as the scaffold for the staples (IDT) ...
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bioRxiv - Biophysics 2021Quote: ... of 0.1M sodium hydroxide: 0.02M 2-(n-morpholino) ethanesulfonic acid (MES) or 1X NEB DNase I reaction buffer (NEB B0303S ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was prepared from total RNA using the rflP specific primer (P-LCM621) and template-switching oligonucleotide (P-LCM619) using RT Template Switching Enzyme Mix (New England Biolabs). The 5’-end end of the rflP cDNA was enriched by PCR using the primers P-LCM620 and P-LCM622 ...
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bioRxiv - Neuroscience 2022Quote: ... p-GSK-3β (S9) (NEB D85E12, 1:50,000), GSK-3β (NEB 27C10 ...
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bioRxiv - Bioengineering 2024Quote: ... containing 0.06 % pluronic acid (F-127, final concentration) and 1 µL of each disulfide bond enhancer 1 and 2 (NEB #E6820). We used droplet oil consisting of 3M™ Novec™ HFE7500 Engineered Fluid (3M ...
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bioRxiv - Neuroscience 2023Quote: ... and p{ywing2+} were combined by HiFi assembly (NEB). The product was transformed into DH5α Chemically Competent Cells (ThermoFisher ...
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bioRxiv - Plant Biology 2024Quote: The hydrolysis rate of p-nitrophenyl phosphate (pNPP, NEB) was determined based on (Wu et al. ...
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bioRxiv - Neuroscience 2020Quote: TEV protease (TEV-P) was purchased from NEB (Cat#P8112S). PFFs were prepared as above and premixed 1:10 (for a final concentration of 15 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’P was created by T4 PNK treatment (NEB, M0201S), done according to the NEB T4 PNK protocol ...
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bioRxiv - Biochemistry 2021Quote: ... Dephosphorylated Ycf1 for these experiments was generated by treating SEC-purified Ycf1 with Lambda phosphatase (Lambda PP, NEB) for 45 min at 4°C as previously described for CFTR26 ...
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bioRxiv - Microbiology 2024Quote: Reverse transcription was carried out with 8 μl of total nucleic acid added to 2 μl of NEB Lunascript RT Supermix (New England Biolabs Cat no. E3010). Nuclease free water was used as a no template control (NTC ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellets containing intact nuclei were vortexed at maximum setting for 15 sec with the nuclear extraction buffer (NEB) and then incubated at 4°C for 30 min with gentle mixing ...
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bioRxiv - Biochemistry 2023Quote: ... formic acid was purchased from Biolabs ltd ...
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bioRxiv - Microbiology 2020Quote: ... we used a colorimetric assay using p-Nitrophenyl phosphate (pNPP) (NEB) as a substrate ...
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bioRxiv - Synthetic Biology 2022Quote: Rectangle: 10 nM of M13mp18 ssDNA scaffold (Bayou Biolabs, P-107) was thermo-ramped in 20x excess of ssDNA staples strands ...
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bioRxiv - Cell Biology 2021Quote: ... Homology arms were then cloned into an SEC backbone plasmid (pJF250, pDD282, or pLC019) with Gibson assembly (NEBuilder HiFi DNA Assembly Master Mix, NEB). A mixture of the modified SEC repair plasmid (25 ng/μl ...
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bioRxiv - Microbiology 2020Quote: ... RNA was fragmented via ultrasound (4 pulses a 30 sec, 4 °C) and subsequently treated with T4 Polynucleotide Kinase (NEB). Half of the samples were then treated with terminator exonuclease (TEX ...
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bioRxiv - Molecular Biology 2023Quote: ... pooled total RNA was fragmented with ultrasound (4 pulses of each 30 sec at 4°C) and then treated with T4 Polynucleotide kinase (NEB). The RNA of each sample was then divided in half ...
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bioRxiv - Microbiology 2024Quote: ... the total RNA samples were fragmented using ultrasound (4 pulses of 30 sec at 4°C) followed by a treatment with T4 Polynucleotide Kinase (New England Biolabs). The RNA samples were then split into two halves and one half was subjected to Terminator Exonuclease treatment (+TEX) ...
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bioRxiv - Biophysics 2024Quote: ... On-column phosphatase treatment was performed by incubating the resin with 10 mL SEC buffer supplemented with 1 mM MnCl2 and 4,000 units (10 μL) Lambda Protein Phosphatase (LPP) (New England BioLabs P0753L) standing at 4°C overnight ...
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bioRxiv - Genomics 2024Quote: ... followed by a nested PCR reaction (5 cycles for 70 sec at 72◦C) with individual sample-barcoded i5 and i7 Illumina TruSeq primers (New England Biolabs NEBNext Multiplex Oligos for Illumina ...
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bioRxiv - Biophysics 2024Quote: ... Nucleic acid was treated with DNase (NEB), purified using RNA SPRI beads ...
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bioRxiv - Microbiology 2020Quote: ... the source plasmid (p-retronWT) was mutagenized through PCR using a kit (NEB; Q5-Site-Directed Mutagenesis Kit ...
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bioRxiv - Plant Biology 2022Quote: ... and 6 µM p-Nitrophenyl Phosphate (pNPP) (New England Biolabs Catalog Number P0757S) at 25°C at a pH of 5.0 ...
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bioRxiv - Microbiology 2024Quote: A colorimetric assay using the substrate p-Nitrophenyl phosphate (PNPP) (New England Biolabs) was used to quantify alkaline phosphatase activity in colonies grown on MOPS agar plates with no added BCIP using the inoculation regime described above ...
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bioRxiv - Neuroscience 2022Quote: ... 601bp and 722bp homology arms flanking the N-terminus of msi-1 were PCR amplified from N2 genomic DNA and inserted into the mNG^SEC&3xFLAG vector pDD283 using NEBuilder Hifi DNA assembly (New England Biolabs, Ipswich, MA). The Cas9 target site was selected using the Sequence Scan for CRISPR database (http://cistrome.org/SSC/ ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μL GlycoBuffer 2 10X (NEB), 2 μL NP-40 ...
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bioRxiv - Cell Biology 2023Quote: ... gel extracted using Monarch Nucleic Acid Purification Kits (NEB) and submitted for Sanger Sequencing ...
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bioRxiv - Cancer Biology 2024Quote: ... 1X Non-Essential Amino Acids (NEAA, #SH30238.01, Nordic Biolabs), 2mM L-Serine (#S4311 ...
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bioRxiv - Neuroscience 2020Quote: ... and annealed (denaturation at 95°C for 5 min and then slow cooling of the samples at the rate of -2°C/sec from 95°C to 85°C and then - 0.1°C/sec from 85°C to 25°C for annealing of heteroduplexes) before addition of T7 Endonuclease I (NEB, Ipswich, Massachusetts, USA, M0302S). Heteroduplexes containing small mutations at the intended site should be cleaved into two fragments ...
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bioRxiv - Genomics 2021Quote: ... to 3’-P presenting in the RNA that was dephosphorylated using T4 PNK (NEB, #M0201S). Linker and RNA ligation was performed by ligating pre-adenylated linker to the 3’-OH of RNA using RNA ligase2 ...
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bioRxiv - Bioengineering 2023Quote: ... Rectangle DNs were folded with 10 nM of M13mp18 ssDNA scaffold (Bayou Biolabs, P-107) and annealed in 20x excess staples ...
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bioRxiv - Genomics 2024Quote: ... to 3’-P presenting in the RNA that was dephosphorylated using T4 PNK (NEB, #M0201S). Linker and RNA ligation was performed by ligating pre-adenylated linker to the 3’-OH of RNA using RNA ligase2 ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μl GlycoBuffer 2 (10X) (NEB, B3704S), 2 μl 10% NP-40 (NEB ...
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bioRxiv - Plant Biology 2022Quote: Nucleic acids were digested using the Nucleoside Digestion Mix (NEB) with the following modifications ...
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bioRxiv - Synthetic Biology 2020Quote: ... the Monarch Nucleic Acid Purification Kits (T1020 and T1030, NEB) were used ...