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Citations for New England Biolabs :
1 - 50 of 2080 citations for 2 6 Amino 9H purin 9 yl ethanol d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Truncated EndoS1 lacking the first 9 amino acids was created by Q5 mutagenesis (New England Biolabs, NEB) by amplifying the full-length construct using primer pairs P154/P155 ...
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bioRxiv - Biochemistry 2024Quote: ... Truncated EndoS1 lacking the first 9 amino acids was created by Q5 mutagenesis (New England Biolabs, NEB) by amplifying the full-length construct using primer pairs P154/P155 ...
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bioRxiv - Microbiology 2020Quote: ... and performed 6–9 cycles of PCR with the NEBNext Ultra II Q5 Master Mix (NEB) using Illumina P7 and the Seq-Well P5-TSO hybrid primer (Gierahn et al. ...
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bioRxiv - Genomics 2021Quote: Purified RNA (9 μg) was reverse transcribed using Random Primer 9 (NEB) and SuperScript II reverse transcriptase under error prone conditions as described in Smola et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... Precipitated DNA was washed with 70% ethanol and resuspended in 1X NEBuffer #2 (B7002S, New England Biolabs) with 2 μl RNase A (20 mg/ml ...
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bioRxiv - Developmental Biology 2024Quote: ... Exon 9 of daf-2 was amplified with Phusion High-Fidelity DNA polymerase (New England Biolabs, M0530S) using primers F_Primer ...
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bioRxiv - Immunology 2021Quote: ... 9 U RNase Inhibitor (NEB) and 90 U Superscript II (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... A mixture containing 6 µl NEBuffer 2 (New England Biolabs, B7002S), 2 µl Klenow enzyme (5 units/µl ...
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bioRxiv - Microbiology 2023Quote: ... 6 ng of sample was treated with 2 units T4 Polynucleotide Kinase (NEB) and incubated at 37°C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were generated by 7-9 rounds of PCR amplification using NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541L), purified using SPRIselect reagent kit (Beckman Coulter ...
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bioRxiv - Systems Biology 2024Quote: ... We digested ∼2ug of each of 24 barcoded ORF plasmid pools (2 replicate pools of each of 9 hORFs and 3 vORFs) overnight with I-SceI (NEB) according to manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... and PCR amplified for 7-9 cycles depending on the samples using NEBNext High Fidelity 2× PCR master mix (New England Biolabs). The optimal cycle number was determined empirically each time by qPCR ...
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bioRxiv - Developmental Biology 2021Quote: ... 9 U of T4 Polynucleotide Kinase (NEB) and 1 U of Klenow fragment (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... 9 U of T4 Polynucleotide Kinase (NEB) and 1 U of Klenow fragment (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... 9 units of T4 Polynucleotide Kinase (NEB) and 1 unit of Klenow fragment (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... 9 U of T4 Polynucleotide Kinase (NEB) and 1 U of Klenow fragment (NEB) ...
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bioRxiv - Developmental Biology 2022Quote: ... 9 U of T4 Polynucleotide Kinase (NEB) and 1 U of Klenow fragment (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligos for side 1 and 2 were dimerized separately by mixing 9 μl of OligoA at 100 μM with 9 μl of OligoB at 100 μM and 2 μl of 10x DNA Ligase Buffer (NEB, M0202S) and heating to 95 °C for 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... and random 9-mer primers (New England Biolabs). Quantitative reverse transcriptase–PCR was performed using SYBR Green Master Mix (Roche ...
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bioRxiv - Biophysics 2023Quote: ... precipitated with ethanol and digested overnight with BsaI (NEB # R3733S). Digested biotinylated arms were purified by spin column chromatography using MicroSpin S-400 HR (Amersham # 27514001).
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bioRxiv - Microbiology 2021Quote: ... 9 Neuraminidase A (P0722, NEB, 4 units/µg protein). 10 µg glycoprotein ...
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bioRxiv - Biochemistry 2024Quote: ... 9 mU/μL Escherichia coli RNA polymerase holoenzyme (NEB), 10 μM rNTPs (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... BS3-d0/BS3-d4 ((bis(sulfosuccinimidyl)suberate-d0/d4, Thermoscientific, Ref. 21590 and 21595), DSBU (Disuccinimidyl Dibutyric Urea, Thermoscientific, Ref. A35459, SNAP-Cell® Block (New England Biolabs Ref. S9106S), glutaraldehyde ...
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bioRxiv - Neuroscience 2024Quote: ... tube 2: 20 µL VLP suspension + 6 µL water + 1 µL Proteinase K (200 µg/mL, NEB), and tube 3 ...
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bioRxiv - Microbiology 2020Quote: ... Purified RNAs were random primed using Random Primer 9 (NEB) by incubation at 65°C for 5 minutes followed by rapid cooling on ice ...
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bioRxiv - Biochemistry 2023Quote: ... pMCM8-9 was dephosphorylated with λ-phosphatase (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... Each transcription reaction consisted of 9 mM rNTPs (NEB N0466), 10 mM Dithiothreitol (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1X Non-Essential Amino Acids (NEAA, #SH30238.01, Nordic Biolabs), 2mM L-Serine (#S4311 ...
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bioRxiv - Genomics 2023Quote: ... and 200 ng/µl Cas 9 protein (New England BioLabs, M0386T). Injected individuals were screened (see below genotyping procedure ...
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bioRxiv - Genetics 2020Quote: ... A 6 µL mixture containing 2 µL of 40 µM Spy Cas9 NLS protein (New England Biolabs, MA, USA), 200 ng each of five sgRNAs (in 2 µL ...
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bioRxiv - Molecular Biology 2022Quote: ... Butyryl-Histone H3 (Lys 9) rabbit pAb (PTM Biolabs, SKU: PTM 305), Butyryl-Histone H3 (Lys 23 ...
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bioRxiv - Genomics 2021Quote: Purified RNA (5 ug) was reverse transcribed using Random Primer 9 (NEB) and SuperScript II reverse transcriptase under error prone conditions as described Smola et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... before 9 μl of 5 U/μl thermostable RNase H (M0523, NEB) were added and samples were incubated 1 h at 50 °C with 800 rpm shaking (15 s on/15 s off) ...
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bioRxiv - Molecular Biology 2023Quote: ... 9 μl of 5 U/μl of thermostable RNase H (M0523, NEB) were added and samples were incubated 1 h at 50 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 µL 200 ng/µL random nonamer (Random Primer 9, NEB). This mixture was heat denatured at 98° C for 1 minute ...
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bioRxiv - Plant Biology 2024Quote: ... 2 µg of total RNA was circularised in a reaction containing 6 U T4 RNA Ligase 1 (New England Biolabs), 50 µM ATP ...
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bioRxiv - Biochemistry 2020Quote: ... first by chloroform extraction and ethanol precipitation followed by Monarch RNA cleanup kit (NEB).
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bioRxiv - Plant Biology 2024Quote: ... The RNA was ethanol precipitated and phosphorylated with T4 polynucleotide kinase (New England Biolabs) prior to library construction using the NEXTFLEX Small RNA-Seq Kit v4 (Perkin Elmer) ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5 μL of SYTO-9 dsDNA dye (NEB BioLabs, for LAMP fluorescent curve) was used to monitor the real-time amplification signals ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5 μL of SYTO-9 dsDNA dye (NEB BioLabs, for LAMP fluorescent curve) was used to monitor the real-time amplification signals ...
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bioRxiv - Molecular Biology 2024Quote: ... 6 and 4 μL of 6× Gel Loading Dye (B7025S, New England Biolabs) and 1.5 and 1 μL of 2.5 mg/mL EtBr were added ...
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bioRxiv - Biochemistry 2020Quote: ... Cross-links were reversed from eluted chromatin by adding 6 μL of 5 M NaCl and 2 μL Proteinase K (NEB; P8107S) and incubation overnight at 65°C ...
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bioRxiv - Cell Biology 2022Quote: ... The RNA/ethanol mixture was transferred to a miniprep kit RNA purification column (NEB, T2010S), and RNA was isolated according to the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... The ethanol precipitated DNA constructs were digested with either HpaII or HhaI (New England BioLabs), which are methylation sensitive restriction enzymes ...
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bioRxiv - Developmental Biology 2024Quote: ... dissociated cells were ethanol-fixed and incubated with 1:100 anti-H3K27me3 antibody (NEB, 9733S) as described previously (34) ...
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bioRxiv - Bioengineering 2020Quote: ... Single amino acid substitutions were introduced utilizing the KLD Enzyme Mix (NEB) following PCR amplification with mutagenic primers (Genewiz) ...
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bioRxiv - Cancer Biology 2021Quote: ... Biotinylated nucleotides from the non-ligated DNA ends were removed by incubating the Hi-C libraries (2 μg) in the presence of 6 U of T4 DNA polymerase (NEB; Cat#: M0203L) in NEBuffer 2.1 supplied with 0.025 mM dATP (Thermo Fisher ...
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bioRxiv - Plant Biology 2023Quote: ... The up- and downstream flanks were obtained by PCR on gDNA of IPO323 with primer pairs 1&2 and 5&6 (Table S7) respectively using Q5 Hot Start High fidelity DNA polymerase (NEB, Evry, France). The hph was amplified from pCAMBIA0380 with the primers 3 and 4 (Table S7) ...
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bioRxiv - Microbiology 2021Quote: ... purple (6×) (New England Biolabs). Samples were loaded into a 4-15% precast polyacrylamide gel ...
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bioRxiv - Molecular Biology 2020Quote: ... Random Primer 6 (NEB, #S1230S), RNasin Ribonuclease inhibitor (Promega ...