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Citations for New England Biolabs :
1 - 50 of 4849 citations for 1 Ethylbenz cd indol 2 1H ylidene malononitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... followed by 1h DpnI (NEB) treatment at 37°C plus inactivation at 80°C for 20 minutes (1 μL enzyme per 50 μL PCR reaction) ...
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bioRxiv - Molecular Biology 2023Quote: ... zHmgn2 CDS-FLAG and oHmgn2 CDS-FLAG were cloned into a pCS2 vector using NEBuilder HiFi DNA Assembly (New England Biolabs). The mRNAs for these constructs were synthesized from linearized plasmids using the mMessage mMachine SP6 Kit (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... 700 bp upstream and downstream were amplified using the A–B and C–CD primer pairs from Table 2 (Phusion polymerase, GC buffer, New England Biolabs). The 2×myc tag was added to the B primers as overhangs ...
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bioRxiv - Molecular Biology 2023Quote: ... the pUbi63E-KDM5-HA plasmid was generated by replacing the Flag-Ubiquitin CDS sequence with the KDM5-HA CDS sequence using Gibson assembly technology provided by NEB (# E5510S).
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bioRxiv - Plant Biology 2022Quote: ... including the stop codon and (2) the CDS and native promoter region 1024 bp upstream using Phusion High Fidelity DNA polymerase (NEB, USA) and TA-ligated into the entry vector pCR8/GW/TOPO (Thermo ...
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bioRxiv - Plant Biology 2021Quote: ... pTNT-CDS vectors were generated through Gibson assembly (NEB).
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bioRxiv - Developmental Biology 2023Quote: ... H2B CDS was obtained from pSNAPf-H2B (New England Biolabs). CDS of Ol-RanT27N and miRFP670-Nano3 was synthesized by eurofin ...
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bioRxiv - Microbiology 2023Quote: ... or deglycosylated by an 1h treatment with PNGase F (NEB) then eluted in 2X Laemmli as previously described.
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bioRxiv - Synthetic Biology 2021Quote: ... Mixes were subsequently digested 1h at 37 °C with DpnI (NEB) and used for electroporation in E ...
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bioRxiv - Plant Biology 2021Quote: ... and MRE11 CDS product were then merged using NotI and NdeI (NEB) restriction cloning to form the MR cassette (Supplementary Figures 13b and 12b) ...
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bioRxiv - Synthetic Biology 2021Quote: The recombinant CBX8-CD was expressed in BL21 (DE3) (New England Biolabs) Escherichia coli cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Endo H digestion was performed at 37°C for 1h in the presence of 1X GlycoBuffer 3 and 1 µL of Endo H enzyme (NEB). The lysates were boiled and subjected to SDS-PAGE and western transfer ...
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bioRxiv - Bioengineering 2024Quote: ... a ligation reaction (1h at room temperature) with T4 DNA ligase (NEB) was performed using a 3:1 cap insert to vector molar ratio ...
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bioRxiv - Cell Biology 2024Quote: ... PCR reactions were digested with DpnI (New England Biolabs, 37°C, 1h) and column purified with standard kits (NucleoSpin from Macherey-Nagel or PureLink from Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... CDS of ATG6 was cloned into the pMal-C2X vector (New England Biolabs) for MBP fusion using infusion cloning (Takara Korea) ...
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bioRxiv - Bioengineering 2021Quote: ... 2 μL of 2 U L-1 ExoI (NEB) was added and incubated at 37 °C for 30 minutes then 80 °C for 20 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... The AB and CD fragments were ligated with T4 DNA ligase (New England Biolabs) (1:1 molar ratio ...
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bioRxiv - Biochemistry 2021Quote: ... The AB and CD fragments were ligated with T4 DNA ligase (New England Biolabs) (1:1 molar ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was phosphorylated for 1h at 37°C with T4 Polynucleotide kinase (NEB or made in-house by the Molecular Biology Service ...
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bioRxiv - Microbiology 2021Quote: ... in 1× NEBuffer 2 (NEB) at 37°C for 1 hour ...
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bioRxiv - Systems Biology 2020Quote: ... The resulting PCR products were digested for 1h at 37°C with DpnI (NEB) and transformed in competent Escherichia coli MC1061 cells ...
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bioRxiv - Synthetic Biology 2023Quote: ... Backbones were linearized with PCR or with restriction enzymes (NEB, 1h at 37°C). PCR-amplified or digested products were purified (Monarch PCR & DNA Cleanup Kit ...
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bioRxiv - Plant Biology 2023Quote: ... Digested DNA was dephosphorylated for 1h by 10U of antartic phosphatase (New England Biolabs). DNA adaptator was ligated overnight with 2,000 u of T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Cell Biology 2023Quote: ... in 1 x GlycoBuffer-2 (Biolabs). After incubation for 60 min at 37 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... (2) 1 µL of DpnI (NEB) was added and incubated at 37°C for two hours ...
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bioRxiv - Genetics 2023Quote: ... The T7 promoter and mtdTomato CDS were amplified from pmrPTRE-AAV using PTRE_floxed_F/R and Phusion High-Fidelity PCR Master Mix (NEB). NotI and SalI sites added by the primers were used to subclone this amplicon into pRM1506_TMM432 ...
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bioRxiv - Genomics 2023Quote: ... an exonuclease treatment was carried out at 37°C for 1h with 10U of exonuclease I (NEB) in 1X exonuclease buffer (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μl 1M MgCl2 and 2 μl of 1 U/μl Xrn1 (M0338, NEB) were added after RNase H digest ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl 10x NEBuffer 1 (New England Biolabs), 2 μl 50 mM MnCl2 ...
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bioRxiv - Cell Biology 2021Quote: ... was cloned into phCMV3 vector by assembling two CDS fragments amplified from phCMV3-CatSpertS:WT (phCMV3-CatSpertS:Mut) using NEBuilder® HiFi DNA Assembly Kit (NEB). ORF sequences of CatSperτS:WT (phCMV3-CatSpertS:WT ...
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bioRxiv - Molecular Biology 2023Quote: ... Deletion variants of ATI2 were amplified from ATI2 (AT4G00355.1) CDS by overlap extension PCR using Phusion High Fidelity DNA polymerase (NEB) and subcloned into pCR™4/TOPO™ vectors (Invitrogen) ...
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bioRxiv - Synthetic Biology 2022Quote: ... An Esp3I restriction site was introduced immediately upstream of the CHIPΔTPR CDS and GSGSG linker via the KLD Enzyme Mix (NEB) following PCR amplification with mutagenic primers (Genewiz) ...
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bioRxiv - Plant Biology 2024Quote: ... Supernatant was recovered after centrifugation (12000 xg, 4°C, 1h) and incubated with 0.002% equilibrated amylose resin (NEB) for 2h on a rotating wheel at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl 10 mM dNTPs and 1 μl Bst 2 WarmStart DNA polymerase (NEB M0538S) was added and sample incubated 30 min at 65°C before precipitation with 12.5 μl 10 M NH4OAc ...
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bioRxiv - Neuroscience 2024Quote: ... 2 ml of virus was incubated with 1 ml DNase I (NEB, 2 units/ml), 4 µL DNase I buffer ...
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bioRxiv - Bioengineering 2022Quote: ... 1X of a restriction enzyme mix [1/2 EcoRI-HF® (NEB #R3101, 2/5 Nuclease-free water, 1/10 CutSmart Buffer 10X (NEB)] ...
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bioRxiv - Microbiology 2021Quote: ... Restriction enzyme-cut pTOX3 was incubated with purified AB and CD PCR products along with a half-reaction of HiFi DNA Assembly Master Mix (NEB) according to manufacturer’s directions ...
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bioRxiv - Bioengineering 2020Quote: ... Respective peptide DNA coding sequences (CDS) were amplified from hACE2 via PCR and inserted using HiFi DNA Assembly Master Mix (NEB) for Gibson Assembly into the pcDNA3-SARS-CoV-2-S-RBD-Fc backbone linearized by digestion with NheI and BamHI ...
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bioRxiv - Microbiology 2020Quote: ... A plasmid containing Ptet-sigB was generated by cloning the 630Δerm sigB CDS amplified with oWKS-1498 and oWKS-1499 in pMiniT (NEB E1202) per manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... CDS plus 7–9 bp of untranslated sequence were amplified using High Fidelity Phusion Taq (New England BioLabs, NEB, USA), 3% DMSO vol/vol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... CDS plus 7–9 bp of untranslated sequence were amplified using High Fidelity Phusion Taq (New England BioLabs, NEB, USA), 3% DMSO vol/vol ...
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bioRxiv - Cell Biology 2021Quote: ... Gene specific primers with restriction sites added to the 5’ end were used to amplify the full CDS or gene fragments using Q5 DNA Polymerase (NEB). Primer sequences and restriction sites used for each construct can be found in Table S8 ...
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bioRxiv - Cell Biology 2020Quote: ... pCS2-luciferase CDS or pCS2-luciferase with G3BP1 5’UTR and 3’UTR were linearized with Sal I (New England Biolabs) and gel purified (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: The EEF1A2 CDS was cloned into pcDNA3.1-FLAG (gift from Saunders Lab) using NEBuilder® HiFi DNA Assembly Cloning Kit (NEB) according to the protocol of the manufacturer ...
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Ferroportin Depletes Iron Needed for Cell Cycle Progression in Head and Neck Squamous Cell CarcinomabioRxiv - Cancer Biology 2022Quote: ... The pLVX-TetOne-puro vector was digested with BamHI and EcoRI and combined with the FPN CDS using NEB HiFi DNA assembly master mix (New England Biolabs) to create the pLVX-tet-FPN vector ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cRFXa CDS was amplified from cDNA using primers MC252 and MC253 and Q5 DNA polymerase (NEB Cat. No. M0491L), with 2 µl of cDNA template in a 50 µl PCR reaction and 35x cycles ...
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bioRxiv - Genetics 2022Quote: SLITRK3 constructs were cDNA synthesised by Genescript (USA) into pCMV-3Tag-4A by replacing the CDs for these inserts via using restriction enzymes BamHI and XhoI (NEB), giving rise to all SLITRK3 plasmid variants ...
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bioRxiv - Synthetic Biology 2023Quote: ... was replaced with a Renilla luciferase CDS via Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) to generate a dual-luciferase reporter containing luc2P and Renilla expression cassettes (pGL4.33R) ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were washed in CutSmart buffer, and incubated (1h, RT) in 150 µL blunting mix (112.5 µL ddH2O, 15 µL 10x blunting buffer, NEB, 15 µL 100 µM dNTPs ...
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bioRxiv - Synthetic Biology 2023Quote: ... the parts were assembled with Gibson assembly (NEBuilder HiFi DNA Assembly Master Mix from NEB, 1h at 50°C) with the linkers providing sequence overlaps ...