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Citations for New England Biolabs :
151 - 200 of 5585 citations for 1 2 Bis Pentabromophenyl Ethane Unlabeled 25 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... 10% Dextran sulfate Sigma D8906; 0.02% BSA Ambion AM2616; 1 mg/ml E.coli tRNA Sigma R1753; 2 mM Vanadyl-ribonucleoside complex NEB S1402S; 2XSSC) containing diluted probes and incubated over night at 30°C ...
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bioRxiv - Plant Biology 2022Quote: ... The cell lysate was collected and incubated for 1 h with 2 ml of 50% slurry of chitin resin (New England Biolabs) before loading onto an empty EconoPac gravity-flow column (Bio-Rad Laboratories ...
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bioRxiv - Genomics 2022Quote: ... for 2 hours and then crosslinked with p19 siRNA binding protein (1 μg/ml in depc-PBS; New England Biolabs) or anti-N6-methyladenosine (m6A ...
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bioRxiv - Molecular Biology 2023Quote: ... Clarified lysate was applied to Glutathione Sepharose 4B affinity resin (1 ml bed volume per 2 l culture; New England Biolabs), and incubated with rotation for
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bioRxiv - Genomics 2019Quote: ... The embryos were treated with protease (1 µL of 25 µg/µL Qiagen Protease, 1 µL of 10x NEB Buffer 4 ...
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bioRxiv - Biophysics 2021Quote: ... 25 μL of PCR reaction was mixed with 1 μL of DpnI (NEB, cat#R0176) at 37°C for 4 hours ...
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bioRxiv - Biophysics 2019Quote: ... 25 µL of PCR reaction was mixed with 1 µL of DpnI (NEB, cat# R0176), 1 µL of T4 PNK (NEB ...
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bioRxiv - Genomics 2022Quote: ... Each “PCR#1” reaction contained 25 µL of Q5 High-Fidelity 2X Master Mix (NEB), 2.5 µL of Nuc PCR#1 Fwd Primer (10 µM) ...
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were undertaken in 25 ul consisting of 1 x Long amp Taq buffer (NEB), 300 μM dNTP mix ...
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bioRxiv - Microbiology 2021Quote: ... acnes lysates were incubated with 0.5 ug plasmid DNA together with different buffers (NEB) supplemented with or without ATP or SAM ...
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bioRxiv - Genomics 2021Quote: Restriction digestion was carried out by adding 25 μL of 10 ×NEBuffer 2 and 100 U of the MluCI restriction enzyme (NEB, R0538) and incubating for ≥2 hours at 37°C in a Thermomixer at 900 rpm ...
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bioRxiv - Immunology 2021Quote: ... Add 50 μl of 10X NEB Buffer 2 and 375 U (15 μl of 25 U/ μl) of MboI restriction enzyme (NEB, R0147), and digest chromatin for 2 hours at 37°C with rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.5 uL each 10 uM forward and reverse primers (cTF223, cTF218 - see Supp. Table 2) and 25 uL NEBNext Q5U Master Mix (NEB, M0597) and ran the following thermocycling protocol ...
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bioRxiv - Microbiology 2021Quote: ... Then homogenized lysate incubated with 0.80 mL of pre- equilibrated oligo(dT)25 magnetic beads (New England Biolabs, #S1419S) for 1 h at 4°C with gentle rotation ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were treated with 25 µg Proteinase K (1.25 µL of 20 mg/mL stock solution, New England Biolabs) and incubated at 50 °C for 20 minutes ...
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bioRxiv - Biophysics 2024Quote: ... Then the suspension was incubated at room temperature for 1.5 h after adding apyrase (25 mU/mL; New England Biolabs). The supernatant was removed by ultracentrifugation at 40,000 rpm for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... after which 2 µL calf intestinal phosphatase (10,000 U/mL, NEB) was added to the digest and incubated for 1h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... An additional 2 μl RNase A (20 mg/ml, NEB #T3018) was added and followed by 5 min incubation at 56 °C to remove residual RNA in the sample ...
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bioRxiv - Genetics 2023Quote: ... diluted to 2 mg/ml in Nuclease-Free Water (NEB, B1500L), at 37°C for 15 minutes each ...
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bioRxiv - Cell Biology 2019Quote: ... stained with oligo-conjugated WGA at a concentration of 2-5 μg/mL in 1× HBSS with 2000× diluted murine RNase inhibitor (New England Biolabs, M0314L) at 37 °C for 20 min ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5XSSC in H2O) on a light table for 1 hour and treated with 2 μg/mL Protease K (NEB, Cat PB107S) in PBS containing 0.3% Triton-X for 10 minutes followed by post-fixation in 4% PFA for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... A 1 ml aliquot of cells was incubated with 1 μl M13KO7 (1011 plaque-forming units per ml, pfu/ml) (NEB) for 10 min on ice to allow attachment ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μl 1M MgCl2 and 2 μl of 1 U/μl Xrn1 (M0338, NEB) were added after RNase H digest ...
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bioRxiv - Microbiology 2021Quote: ... PCRs were performed in 25 μL reaction volumes and contained 13 μL Q5® Hot Start High-Fidelity 2× Master Mix (New England Biolabs, US), 0.5 μM of each primer and 0.4 μL of 25 mg/mL BSA ...
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bioRxiv - Genomics 2021Quote: ... 25 ng of cDNA was digested with 0.5 µl 1:40 diluted RNaseH (New England Biolabs) for 20 min at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 µl aliquots were 5’ adapter ligated (25 µl of 1× T4 RNA ligase buffer (NEB), 1 mM ATP ...
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bioRxiv - Molecular Biology 2023Quote: ... Four ug of fragmented gDNA was digested with 20 U of RNase H (NEB #M0297S) at 37°C for 6 hours to serve as a negative control ...
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bioRxiv - Cell Biology 2021Quote: ... 1% NP-40, 1 mM DTT, 0.1 mg/ml cycloheximide, 1.25 mg/ml heparin, 400 U/ml NEB murine RNase inhibitor) and dounced ...
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bioRxiv - Genomics 2020Quote: rNTP mix @ 25 mM each (NEB)
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bioRxiv - Immunology 2021Quote: ... at 25 °C (Creative-Biolabs Inc.). RNA-1 conjugated with biotin (synthesized by IDT lnc. ...
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bioRxiv - Molecular Biology 2020Quote: ... 18 μL 25 mM dNTPs (NEB), 30 μL 0.1 M DTT (Invitrogen) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Reagents added were 1 μl of 1 mg/ml BSA (diluted from 20 mg/ml - NEB), 1 μl T4 DNA ligase buffer (NEB or Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl 10x NEBuffer 1 (New England Biolabs), 2 μl 50 mM MnCl2 ...
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bioRxiv - Cell Biology 2022Quote: ... followed by 2 μL of 20 mg/mL RNaseA (New England BioLabs) for 30 min at 37 °C ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was then passed over a 2 mL amylose column (NEB) and washed with 3 CV of Flag Wash Buffer ...
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bioRxiv - Biochemistry 2020Quote: ... 186 bp DNA (0.4 mg/ mL) in 1x NEBuffer 2 (NEB: B7002) was incubated with dATP (100 μM) ...
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bioRxiv - Biochemistry 2022Quote: ... and further passivated with 2 mg/ml Bovine serum albumin (BSA, NEB:B9000S) to prevent non-specific binding of proteins and DNA to the surface ...
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bioRxiv - Bioengineering 2022Quote: ... followed by 2 μL of 20 mg/mL RNaseA (New England BioLabs) for 30 min at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was rocked with 2 mL of amylose resin slurry (NEB) for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by 2 µL of 20 mg/mL RNaseA (New England BioLabs) for 30 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... 2 μL of T4 RNA Ligase (10 000 U/mL, NEB, M0204S), and 10 μL of PEG 8000 50% (NEB ...
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bioRxiv - Genomics 2022Quote: ... 1 mg/ml Proteinase K (NEB) and 1 mM Biotin
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bioRxiv - Genomics 2022Quote: ... 1% BSA (20 mg/ml, NEB)) + hash oligos (1 uM ...
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bioRxiv - Biophysics 2023Quote: ... Streptavidin (1 mg/mL, NEB N7021S) was diluted 1:10 in DPBS and applied to passivated flow chambers for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl 10 mM dNTPs and 1 μl Bst 2 WarmStart DNA polymerase (NEB M0538S) was added and sample incubated 30 min at 65°C before precipitation with 12.5 μl 10 M NH4OAc ...
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bioRxiv - Cancer Biology 2024Quote: ... and chromatin was digested overnight by adding 25 µL 10X NEBuffer 2 and 100 U Mbo I (New England Biolabs, catalog No. R0147). Digestion efficiency was confirmed by isolating DNA from a 25-µL aliquot of the suspension and performing agarose gel electrophoresis ...
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bioRxiv - Bioengineering 2022Quote: ... 1X of a restriction enzyme mix [1/2 EcoRI-HF® (NEB #R3101, 2/5 Nuclease-free water, 1/10 CutSmart Buffer 10X (NEB)] ...
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bioRxiv - Microbiology 2021Quote: SYBR-based qPCR reactions were set up in duplicate as follows: 25 μl reactions with 20 U/ml Phusion Hot Start Flex DNA polymerase (NEB M0535L), 1X HF Buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... MNase dilutions were prepared fresh before each digestion at 25 μg/mL in 1X bovine serum albumin (BSA) (New England Biolabs B9001S).
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bioRxiv - Genomics 2022Quote: ... 3 ug of input DNA was dephosphorylated with Quick CIP (New England Biolabs, cat no M0508). Following enzyme inactivation with alkaline phosphatase ...