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Citations for New England Biolabs :
4901 - 4950 of 10000+ citations for Rat Insulin Like Growth Factor 1 IGF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Sik3 and Lkb1 were cloned into pXPR-RFP-Blast using the Gibson Assembly Kit (E2611L, NEB). The target sequences are as follows ...
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bioRxiv - Cell Biology 2023Quote: ... total RNA isolation was performed by using the Monarch Total RNA Miniprep Kit (New England BioLabs) and RNA concentration was detected in a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... 2021.9 All three fragments were cloned via Gibson assembly using a HiFi DNA assembly kit (NEB) into a vector backbone containing PiggyBac transposition sites.35 The Cer1:H2B-Venus reporter construct was co-transfected with CAG-pBASE35 using Lipofectamine 2000 (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was derived from total RNA using ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560S). Numerous primers were designed against cryptic exon targets and screened to identify primer pairs that minimized background bands.
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bioRxiv - Molecular Biology 2023Quote: ... The samples were prepared using a Luna Universal One-Step RT-qPCR Kit (New England Biolabs). Quantitative RT-PCR was performed with an Mx3000P qPCR system (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... va mtSSB ΔMTS by PCR amplification and ligation (Q5 Site-Directed Mutagenesis Kit, NEB, Cat #E0554). The plasmid insert was sequence-verified and then transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... libraries were prepared using the Ultra Directional RNA library prep kit for Illumina (New England Biolabs), and normalized libraries of biological triplicates of all samples were sequenced on Illumina’s NextSeq 1000 platform at 2 x 100 bp read length.
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bioRxiv - Cancer Biology 2023Quote: ... All single-cell libraries were prepared using the NEBNext Ultra II FS kit (New England Biolabs) following the manufacturer’s instructions but using one-fourth volumes ...
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bioRxiv - Genomics 2023Quote: ... and the sequencing libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: The mRNAs used in this study were produced using HiScribe T7 mRNA synthesis kit (NEB, Australia) using linearized DNA produced by PCR amplification ...
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bioRxiv - Plant Biology 2023Quote: ... and cDNA was synthesized using the Proto-Script first strand cDNA synthesis kit (New England Biolabs). Quantitative RT-PCR was performed in a CFX Opus Real-Time PCR (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... PCR products were purified using the Monarch® PCR & DNA clean-up kit (New England Biolabs). In vitro expression was performed for 2 h at 37 °C ...
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bioRxiv - Physiology 2023Quote: ... sequencing libraries were generated using NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB, USA). Two-3μg RNA per sample was used to purify mRNA using poly-T oligo attached magnetic beads and then the purified mRNA will be fragmented into short fragments (about 200 bp ...
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bioRxiv - Plant Biology 2023Quote: ... The proGL2:EYFP:gl2ΔNLS construct was created using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs), taking wild-type GL2 cDNA in pENTR/D-TOPO as a template ...
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bioRxiv - Microbiology 2023Quote: ... and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) following the manufacturer’s directions ...
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bioRxiv - Genomics 2023Quote: ... and stranded libraries constructed using the NEBNext Ultra II directional RNA library preparation kit (NEB, E7760) following the protocol for ribosome-depleted RNA and with an 11-min RNA fragmentation step according to the manufacturer’s instructions ...
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Gene expression analyses reveal differences in children’s response to malaria according to their agebioRxiv - Microbiology 2023Quote: ... before preparation of stranded libraries using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB). cDNA libraries were sequenced on an Illumina NovaSeq 6000 to generate ∼55-130 million paired-end reads of 75 bp per sample ...
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bioRxiv - Cell Biology 2023Quote: ... libraries were constructed using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) and NeBNext Multiplex Adapters (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... see below) by site-directed mutagenesis using the Q5 site-directed mutagenesis kit (New England Biolabs) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: RNA-seq libraries were prepared with NEBNext Ultra II Directional RNA-seq kit for Illumina (NEB) after rRNA depletion (NEBNext rRNA depletion kit by NEB ...
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bioRxiv - Neuroscience 2023Quote: ... Sequencing libraries were generated using NEBNext Ultra TM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... The libraries were quantified using the NEBNext Library Quant Kit for Illumina (#E7630; New England Biolabs) based on the mean insert size provided by the Bioanalyzer ...
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bioRxiv - Neuroscience 2023Quote: ... We quantified the libraries using the NEBNext Library Quant Kit for Illumina (New England Biolabs, #E7630) based on the mean insert size provided by the Bioanalyzer ...
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bioRxiv - Bioengineering 2023Quote: ... NEBNext Ultra II Directional RNA Library Prep Kit (Catalog # E7760, NEW ENGLAND BIOLABS,MASSACHUSETTS, UNITED STATES), NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then indexed and adaptor-ligated using NEBNEXT Ultra II DNA Library Preparation Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA-sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep kit for Illumina (NEB) combined with the NEBNext rRNA Depletion kit (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... FlucWT-HA-GFP11 and FlucDM-HA-GFP11 plasmids were constructed using site-directed mutagenesis kit (NEB) based on FlucSM-HA-GFP11 ...
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bioRxiv - Physiology 2023Quote: ... Samples were reversed transcribed using the LunaScript RT SuperMix Kit (New England BioLabs, Ipswich, Massachusetts, USA) and relative gene expression was quantified via CFX-96 Real-Time PCR Detection System (RRID:SCR_018064 ...
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bioRxiv - Cancer Biology 2023Quote: ... the NEBNext Ultra II DNA Library Prep Kit for Illumina was followed (NEB, cat. no. E7645S) using total DNA yield as input material ...
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bioRxiv - Developmental Biology 2023Quote: ... ChIP libraries were prepared by using NEBNext® Ultra™ DNA Library Prep Kit (NEB, E7770L) for Illumina ...
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bioRxiv - Genetics 2023Quote: ... Libraries were prepared using the NEBNext Ultra II kit (New England Biolabs, cat. no. E7645S/L). Index primers set 1 and 2 from the NEBNext Multiplex Oligos for Illumina kit (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... Library preparation was done using the NEBNext® UltraTM II DNA Library preparation kit (NEB, #E7103L). Libraries were sequenced on single-end 50 bp (1×50 bp ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared with the NEBNext Ultra II DNA library prep for Illumina kit (NEB E7645S) and sequenced on an Illumina NextSeq2000.
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bioRxiv - Genomics 2023Quote: RNA was extracted from the cell lysates using the Monarch Total RNA Miniprep kit (NEB T2010) and its quality was spot-checked for random samples using the Bioanalyzer High Sensitivity RNA Kit (Agilent 5067-1513) ...
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bioRxiv - Genomics 2023Quote: ... Library prep was performed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7103) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, Catalog #E7530L) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from ear punch biopsies using the Monarch Genomic DNA purification kit (NEB). Transgenes were identified using PCR primers specific for each transgene (Suppl ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA-seq libraries were then prepared using the NEBNext Ultra II directional RNA library kit (NEB).
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bioRxiv - Molecular Biology 2023Quote: ... followed by library preparation using the NEB Next Ultra II RNA Library Prep Kit (NEB E7770L). Sequencing was done using the Illumina Nextseq with 75-bp read length and single-end.
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bioRxiv - Molecular Biology 2023Quote: ... The pJC53.2_SmUbi-EGFP-SmUbi construct was assembled using the NEBuilder Hifi DNA Assembly Cloning Kit (NEB). Competent Escherichia coli DH5α (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... and the 3,240 bp fragment recovered from the gel (Monarch Gel Extraction Kit, NEB, Ipswich, MA). Components of the transgene construct ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA library preparation was done using NEBNext Ultra II RNA library prep kit (NEB E7770S) as per manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations ...
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: ... Libraries were constructed using NEBNext® Ultra II DNA Library Prep Kit for Illumina (NEB, E7645S). Prepared CUT&RUN libraries of PRC1ctrl and PRC1cKO Aundiff were sequenced on the NovaSeq 6000 system (Illumina ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA was extracted using the Monarch® Genomic DNA Purification Kit (New England Biolabs, Massachusetts; #T3010) with the Monarch® gDNA Tissue Lysis Buffer (#T3011 ...
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bioRxiv - Cell Biology 2023Quote: ... and FLAG-βTrCP2 (WD40Mut) were generated using the Q5 site-directed mutagenesis kit (NEB; Cat. #E0552S). The βTrCP1 WD40Mut changes amino acid 510 from R>A based on isoform 1 in uniprot (R474A when referring to isoform 2 in uniprot) ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were prepared using the NEBNext rRNA Depletion Kit v2 (Human/ mouse) (NEB #E7405), NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760 ...
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bioRxiv - Cancer Biology 2023Quote: ... and libraries for Illumina sequencing were prepared using the NEBNext Ultra II RNA kit (NEB, #E7770L). Quantification of the library was performed using dsDNA HS assay kit and Qubit (Molecular Probes ...
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bioRxiv - Cell Biology 2023Quote: ... mCh-Rab7A was cloned into a piggyBac plasmid using the NEB HIFI DNA Assembly Kit (NEB) and primers listed in Supp ...
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bioRxiv - Developmental Biology 2023Quote: ... Sequencing libraries were generated using a NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, E7770S) following the manufacturer’s recommendations ...