Labshake search
Citations for New England Biolabs :
4851 - 4900 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The ChIP libraries were constructed utilizing NEBNext® Ultra™ II DNA Library Prep Kit (NEB, E7645L) and sequenced on an Illumina NovaSeq 6000 PE150 platform using the 150-bp pair-end sequencing parameters (Novogene ...
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bioRxiv - Cell Biology 2024Quote: ... RNA libraries were constructed utilizing the NEBNext® Ultra RNA Library Prep Kit for Illumina (NEB, E7530L) and sequenced on an Illumina NovaSeq 6000 PE150 platform using the 150-bp pair-end sequencing parameters (Novogene ...
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bioRxiv - Microbiology 2024Quote: ... libraries were prepared using the NEBNext® Ultra™ Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... A repair template plasmid was constructed using Gibson assemble (Gibson et al., 2008) (NEB Hifi assembly kit). The repair construct was made by joining the PfAMA1 coding region and ∼1.5 kb 5’ to the transcription start site amplified from 3D7 genomic DNA ...
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bioRxiv - Neuroscience 2024Quote: ... and effectors were cloned using the Gibson Cloning Assembly Kit (New England BioLabs, catalog no. NEB-E5510S). After cloning and sequencing ...
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bioRxiv - Immunology 2024Quote: ... cDNA was used to make libraries with the NEBNext Ultra II FS Library Preparation Kit (E6177S, NEB), using a five-fold lower reaction volume than instructed by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... 1.4 μM of each purified mRNA was used in PURExpress in vitro Protein Synthesis Kit (NEB#E6800) in 10 μl reaction in 384 well Small Volume black plate (Grenier 788 096) ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was then extracted and purified utilizing the Monarch Total RNA Isolation Kit (New England Biolabs), followed by triple DNase I (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... and effectors were cloned using the Gibson Cloning Assembly Kit (New England BioLabs, catalog no. NEB-E5510S). After cloning and sequencing ...
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bioRxiv - Microbiology 2024Quote: ... DNA libraries were constructed using NEBNext® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: cDNA libraries for sequencing were constructed using TruSeq RNA Library Prep Kits for Illumina® (NEB, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... The oxidised PTC rRNA was subsequently purified from the reaction mixture with Monarch RNA Cleanup Kit (NEB) and immediately mixed with 150 nmol FTSC (300 eq. ...
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bioRxiv - Neuroscience 2024Quote: ... and cDNA was synthesized using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, #E6560L). cDNA was amplified using primer pairs designed to either target two wildtype sequences on either side of the cryptic exon or one wildtype sequence and another sequence directly in the cryptic exon ...
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bioRxiv - Neuroscience 2024Quote: RNA was extracted from samples using the Monarch® Total RNA Miniprep Kit (New England Biolabs, #T2010S) and cDNA was synthesized using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, #E7530L). All libraries were prepared using manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA sequencing library preparation was performed using the NEBNext® DNA Library Prep Kit (New England Biolabs) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... The synthesized probes were then purified using the Monarch® PCR & DNA Cleanup Kit (New England BioLabs) according to the manufacturer’s instructions and the quality was verified on denaturing 12-16% polyacrylamide gels.
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bioRxiv - Cancer Biology 2023Quote: ... First strand cDNA synthesis was performed with LunaScript RT SuperMix Kit (E3010L; New England Biolabs, Ipswich, MA), and 100 ng-1 μg total RNA was used for one 20 μl RT reaction ...
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bioRxiv - Genomics 2024Quote: ... and used for the cloning of PCR fragments with a Gibson Assembly cloning kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Indexed DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB, Cat # E7645S) with the following specifications ...
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bioRxiv - Cancer Biology 2024Quote: ... and library prep were performed in parallel using the Ultra II Directional RNA-Seq Kit (NEB E7760) and reads were sequenced 2x50bp on an Illumina NovaSeq S4.
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bioRxiv - Microbiology 2024Quote: ... SACOL2602 and SACOL2601 were individually deleted from pRab11-tspA1-328-pep86_2603_2602_2601 using the Q5 site-directed mutagenesis kit (NEB). Oligonucleotides are listed in Table S1.
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bioRxiv - Cell Biology 2024Quote: The tricistronic Gs and Gs-CFP plasmids were created using NEBuilder HiFi DNA assembly cloning kit (NEB). Gαs (or Gαs-CFP54) ...
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bioRxiv - Cell Biology 2024Quote: ... and libraries prepared using the NEBNext UltraII Directional RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Ribosomal RNA was depleted from the total RNA using the NEBNext rRNA Depletion kit (New England Biolabs), followed by the conversion of the remaining RNA into an Illumina sequencing library using the NEBNext Ultra Directional RNA Library Prep kit (New England Biolabs) ...
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bioRxiv - Developmental Biology 2024Quote: ... After PCR amplification or plasmid digestion product was purified with Monarch PCR clean-up kit (NEB, #T1030s) and used to produce the DIG-labeled single-stranded RNA probes using the DIG RNA labeling kit (Roche ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of the purified amplicons was quantified by qPCR using the NEBNext Illumina Quant kit (NEB). The forward and reverse reads were demultiplexed and fused barcodes were quantified through alignment against custom barcode and primer sequences using Bowtie2 (V2.3.4.1 ...
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bioRxiv - Immunology 2024Quote: ... Libraries were prepared using the NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs) according to manufacturer’s instructions with the following specifications and modifications ...
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bioRxiv - Immunology 2024Quote: ... DNA library construction was performed using the NEBNext Ultra II DNA Library Prep Kit from NEB (E7645L). The libraries were sequenced on Illumina NovaSeq 6000 system (50 bp paired-end ...
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bioRxiv - Genomics 2023Quote: ... DNase-seq libraries were prepared using the NEBNext Ultra Library Prep Kit for Illumina (New England Biolabs). Illumina sequencing was performed on an Illumina HiSeq instrument to a depth of 12 to 33 million mapped 40-50 bp single-end sequence reads per sample ...
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bioRxiv - Microbiology 2023Quote: ... and used to prepare the library with the Next ultra II DNA library prep kit (E7645, NEB) using 1 μg of DNA per sample as input ...
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bioRxiv - Biochemistry 2024Quote: ... Fragments were assembled into the pBAD24 vector using HiFi DNA Assembly Kit (New England Biolabs, Ipswich, MA) with 1-hour ...
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bioRxiv - Biochemistry 2024Quote: ... The inserts were each ligated with digested pmirGLO plasmids using a quick ligasation kit (New England Biolabs) and transformed into JM109 competent cells (Promega) ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmids were then isolated using the Monarch® Plasmid Miniprep Kit (New England Biolabs, Ipswich, MA, USA) and used to transfect HEK293T cells (ECACC ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was isolated from ∼2×106 cells using the Monarch DNA purification Kit (New England Biolabs). For genotyping ...
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bioRxiv - Genetics 2023Quote: ... and genomic DNA libraries were prepared using the NEBNext genomic DNA library construction kit (New England Biolabs). DNA libraries were sequenced on an Illumina Hi-Seq and deep sequencing reads were analyzed using standard methods on Galaxy ...
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bioRxiv - Cancer Biology 2023Quote: ... The RNA-seq libraries were prepared with Ultra II Directional RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2024Quote: ... All individual PLpro mutations were introduced into pDONR221-PLpro using NEB Q5 Site-Direct Mutagenesis Kit (NEB) following manufacturer’s instructions and shuttled into FU-tetO-Gateway-rtTA-2A-Puro using LR clonase.
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bioRxiv - Molecular Biology 2023Quote: ... Library preparation was done using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEW ENGLAND BioLabs) as described by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... Generation of Adgrd1 N-terminal mutations was carried out using Q5 site directed mutagenesis kit (NE Biolabs) using manufacturers protocol ...
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bioRxiv - Genetics 2023Quote: ... Whole genome sequencing libraries were prepared using the NEBNext Ultra II Library kit (New England Biolabs, E7645L) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: Library construction was carried out using NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645S, NEB). 4ng of fragmented DNA was used for end-repair/A-tailing ...
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bioRxiv - Microbiology 2023Quote: ... and cDNA was generated using an AMV first strand cDNA synthesis kit (New England Biolabs, Catalog # E6550). The segment was amplified through PCR with PstI and BamHI restriction sites incorporated onto the double-stranded cDNA using specific primers (PstI-VIGS-F and BamHI-VIGS-R ...
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bioRxiv - Molecular Biology 2022Quote: ... Ligation of the insert to the vector was performed using the Quick Ligation kit (New England Biolabs). The final vector was transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... The amplicon was inserted into miniTn7/pMQ56 using the NEBuilder HiFi DNA Assembly kit (New England BioLabs) according to the manufacturer specifications ...
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bioRxiv - Microbiology 2023Quote: ... NCPF 8977 (aggregating) and NCPF 13029 (weakly-aggregating) using Monarch RNA extraction kit (NEB cat no. T2010) as per manufacturer’s specification ...
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bioRxiv - Synthetic Biology 2023Quote: ... RNA was purified using a Monarch RNA Cleanup Kit (500 μg) (Catalog No. T2050L, New England BioLabs) and concentration was measured using a NanoDrop ND-1000 UV-Vis Spectrophotometer (Thermo Scientific).
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bioRxiv - Synthetic Biology 2023Quote: ... Quick Blunting Kit and the T4 DNA Ligase enyzme were purchased from New England BioLabs (NEB, Germany). PCR was performed using Q5 High Fidelity 2X Master Mix (NEB ...
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bioRxiv - Bioengineering 2023Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) and index codes were added to attribute sequences to each sample ...