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Citations for New England Biolabs :
4601 - 4650 of 10000+ citations for Human Oxidized low density lipoprotein receptor 1 OLR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The PCR product was purified using the Monarch PCR and DNA cleanup kit (T1030S, NEB) and dsRNA was synthesised using the T7 RiboMAX express system (P1700 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and purified the rest of the digestion product with Monarch Nucleic Acid Purification Kit (NEB). We quantified the amount of purified DNA using the Qubit dsDNA HS Assay (Thermofisher ...
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bioRxiv - Cell Biology 2024Quote: ... The DNAse-digested RNA was purified using the Monarch RNA Cleanup kit (New England Biolabs) as recommended by the manufacturer and used as template for cDNA synthesis and SYBR Green-based qPCR (see quantitative real-time PCR (RT-qPCR ...
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bioRxiv - Microbiology 2024Quote: ... Additional mutations/deletions were introduced in gp140-T/F07 using site directed mutagenesis kit (NEB). The sequences of the recombinant clones were verified through sequencing (Retrogen ...
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bioRxiv - Synthetic Biology 2024Quote: ... Amplification products were gel extracted using the Monarch® DNA Gel Extraction Kit (NEB T1020).
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bioRxiv - Bioengineering 2024Quote: ... PCR reactions were purified using the Monarch DNA PCR Cleanup Kit (New England Biolabs; T1030). For Gibson assembly of constructs ...
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bioRxiv - Cell Biology 2024Quote: ... a NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, E7645S) was used according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (NEB; E7645) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... following the manufacturer’s guidelines provided with the library kit (E7560S, New England BioLabs Inc., USA). Monarch PCR purification kit (Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were extracted from agarose using a Monarch DNA Gel Extraction Kit (NEB, # T1020), quantitated and next generation sequencing was performed using an Illumina NextSeq 75 cycle High Output kit on an Illumina NextSeq platform.
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bioRxiv - Cancer Biology 2024Quote: ... Fragmented DNA samples was purified using the Monarch DNA Clean up Kit (New England Biolabs). The libraries were prepared from 5-10ng purified CUT&RUN-enriched DNA ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs #T2010S). Reverse transcription was performed using iScript Reverse Transcription Supermix (BioRad #1708841 ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA was extracted using Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB, T3050), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... First strand cDNA was synthesized using a Lunascript® RT SuperMix Kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was further processed using the Monarch RNA cleanup Kit (New England Biolabs, Ipswich, MA). Protocol steps were executed according to manufacturer’s instructions with slight modifications and the RNA was finally eluted in a total volume of 40ul of RNase/DNase free water ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were then PCR purified using a Monarch® PCR & DNA cleanup kit (NEB-T1030S) once a band of the correct size was visualized on a 1% Agarose gel ...
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bioRxiv - Biochemistry 2024Quote: All protein mutagenesis was performed with the Q5 site-directed mutagenesis kit from NEB (E0554). Primers were designed with the NEBaseChanger tool (https://nebasechanger.neb.com/ ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA was depleted with the NEBNext rRNA depletion kit for bacteria (New England Biolabs) and a set of 20 pooled sequence-specific probes for Bc ...
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bioRxiv - Biochemistry 2024Quote: ... Libraries were then prepared on beads using NEBNext Ultra II DNA Library Prep kit (NEB) following manufacturers recommendations with 0.6 μM of KAPA Unique Dual-Indexed adapters (Roche) ...
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bioRxiv - Biochemistry 2024Quote: ... The crude RNA was preliminarily purified using Monarch RNA Clean-up kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: In Vitro Translation (IVT) was performed using the PURExpress in vitro protein synthesis kit (NEB). Each IVT reaction contained 4 μL of PURE Solution A ...
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bioRxiv - Plant Biology 2024Quote: ... The concentration was estimated using the NEBNext Library Quant Kit for Illumina (New England Biolabs). Each 10 nM library pool (mim-tRNAseq or QuantM-tRNAseq ...
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bioRxiv - Synthetic Biology 2024Quote: RT-qPCR was conducted using Luna®Universal One-Step RT-qPCR Kit (NEB E3005L) following the manufacturer’s documentation ...
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bioRxiv - Genetics 2024Quote: ... PCR fragments were purified using the Monarch DNA Gel Extraction Kit (New England Biolabs, T1020) and assemblies were performed using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using the Monarch Genomic DNA Purification Kit (New England Biolabs, Whitby, Canada) according to the manufacturer’s recommendations for DNA purification from gram-positive bacteria and Archaea ...
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bioRxiv - Synthetic Biology 2024Quote: mRNA was isolated from total RNA extracts using the magnetic mRNA Isolation Kit (NEB S1550S) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Golden Gate Assembly Kit (BsaI-HF v2, E1601L) and the Gibson Assembly Protocol (NEB E5510) were employed ...
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bioRxiv - Synthetic Biology 2024Quote: RNA was extracted 48h after transfection using the Monarch total RNA miniprep kit (NEB T2010S) and stored at −80C until further processing ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA was depleted using the NEBNext rRNA Depletion Kit for bacteria (New England Biolabs), cDNA libraries were constructed using the NEB Ultra Directional RNA library prep kit for Illumina and sequenced on Illumina’s NextSeq 1000 platform at 2 × 100 bp read length ...
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bioRxiv - Microbiology 2024Quote: ... The in vitro translation assay was performed using PURExpress In Vitro Protein Synthesis Kit (NEB). 24 μM of antibiotics were added individually to the translation mixture and incubated at 37 °C for 4 hours ...
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bioRxiv - Biochemistry 2024Quote: ... The W75K substitution was introduced using Q5 site-directed mutagenesis kit (New England Biolabs, NEB). Substitutions L80E ...
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bioRxiv - Biochemistry 2024Quote: ... The W75K substitution was introduced using Q5 site-directed mutagenesis kit (New England Biolabs, NEB). Substitutions L80E ...
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bioRxiv - Neuroscience 2024Quote: ... The NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs, MA, USA) was performed on the RNA ...
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bioRxiv - Genomics 2024Quote: ... The pooled DNA was PCR-amplified using Phusion® High-Fidelity PCR Kit (NEB®), followed by purification with a Monarch® PCR & DNA Cleanup Kit (NEB®) ...
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bioRxiv - Molecular Biology 2024Quote: ... Y511A mutation in pBAD-His-BrxX was introduced using Q5 site-directed mutagenesis kit (NEB), while introduction of Y511A to the pBREX AL was carried by joining 2 plasmid fragments ...
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bioRxiv - Systems Biology 2022Quote: ... 5’-AAAC(N)19-20-3’) by combining 1 μl of each 100 μM oligonucleotide with 1 μl of 10× T4 ligation buffer (NEB cat. no. B0202S), 6.5 μl of water ...
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bioRxiv - Genomics 2020Quote: ... 50-100 fmol of PCR products were diluted in 9 µl then mixed with 1 µl of Rapid 1D Adapter and 1 µl of Ligase T4 Blunt (New England Biolabs, Ipswich, MA, USA). Pre-sequencing mix was incubated 10 min at 25°C and left on ice until ready to load ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragment release step was performed in 5 μl 1% SDS supplemented with 1:10 Thermolabile Proteinase K (New England Biolabs cat. no. P8111S) at 37°C 1 hr followed by 58°C 1 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragment release step was performed in 5 μl 1% SDS supplemented with 1:10 Thermolabile Proteinase K (New England Biolabs cat. no. P8111S) at 37°C 1 hr followed by 58°C 1 hr ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1 µM of the 6-kb fragment was incubated with 1 unit/µL terminal deoxynucleotidyl transferase (TdT, New England Biolabs, MA, USA, #M0315S) and 0.5 mM deoxyadenosine triphosphate (dATP ...
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bioRxiv - Biochemistry 2023Quote: ... In vitro methylation experiments were performed for 10 min (DNA) or 1 h (RNA) at 37 °C in 8 μL reaction mixtures containing 1× CutSmart buffer (New England Biolabs Inc., Massachusetts, USA) with 20 nM DNA or 50 nM RNA with 1 unit RNase Inhibitor (New England Biolabs Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µL ligation mix (2 µL 10x T4 ligase buffer, NEB, 1 µL T4 RNA ligase 2, truncated, NEB, 1 µL Ribolock inhibitor) was added and incubated (1 h ...
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bioRxiv - Bioengineering 2023Quote: ErCas12a (1 μg) or LbCas12aU (1 μg) protein and 1 μg crRNA were assembled in 1X Nuclease Reaction Buffer (New England BioLabs, Ipswich, MA, USA) at room temperature for 10 minutes ...
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bioRxiv - Plant Biology 2020Quote: ... The amplified NbPRS4 PCR product was gel purified (Monarch DNA Gel Extraction Kit, New England Biolabs), digested with XbaI and XhoI (New England Biolabs) ...
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bioRxiv - Biophysics 2020Quote: ... The insert was further cloned into pcDNA3.4 using the HiFi DNA Assembly Kit (New England Biolabs) and PCR products (Phusion polymerase ...
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bioRxiv - Cancer Biology 2021Quote: ... GSE1 coding sequence was modified using the Q5 Site-directed Mutagenesis Kit (E0554S, New England Biolabs) to substitute the cytosine (C ...
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bioRxiv - Cancer Biology 2021Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNALibrary Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was synthesized from 700ng of amplified oligos using T7 RNA polymerase (HiScribe T7 kit, NEB) following manufactural instruction ...
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bioRxiv - Cell Biology 2019Quote: ... A point mutation (D697A) was introduced with the Q5® Site-Directed Mutagenesis Kit (E0554S NEB). Yeasts were cultured to an OD600 of 4 ...
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bioRxiv - Cell Biology 2019Quote: ... 1μg total RNA was used to synthesise cDNA with the ProtoScript II kit (New England Biolabs) using random primers in a 20μl reaction ...