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Citations for New England Biolabs :
4451 - 4500 of 9360 citations for Rat CD325 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... and resuspended in 20 µL H2O.Preparation of sequencing libraries was performed using NEBNext Ultra II kit (NEB) with 7 PCR cycles for library amplification ...
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bioRxiv - Evolutionary Biology 2023Quote: ... complemented by reagents from the NEBNext Ultra II library prep kit (New England Biolabs, Ipswich, Massachusetts, U.S.A.), and then sequenced at a MinION platform (Oxford Nanopore Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... Then first-strand cDNA was produced using ProtoScript® II First Strand cDNA Synthesis Kit (NEB, E6560s) with random primers ...
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bioRxiv - Cancer Biology 2023Quote: The RNA library was constructed using NEB Next ® Ultra™ RNA Library Prep Kit (NEB, USA), following manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2023Quote: ... and RpoD control were assessed using the Luna Universal One-Step qRT-PCR kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and the DNA library was prepared using the NEBNext® Microbiome DNA Enrichment Kit (New England Biolabs). The sequencing run was then performed on the Element AVITI sequencer (Element Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... . Libraries for Illumina short-read sequencing were prepared using the NEBNext Ultra II RNA library kit (NEB) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The SKP1-FBXO22 mutant (C326A) was generated using a Q5 Site-Directed Mutagenesis Kit (NEB, cat. E0552). Both SKP1-FBXO22 WT and C326A were expressed in BL21(DE3 ...
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bioRxiv - Bioengineering 2023Quote: Complementary DNA (cDNA) was synthesized using the First Strand cDNA Synthesis Kit (New England BioLabs, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... the HiScribe® T7 High Yield RNA Synthesis Kit from New England Biolabs (NEB E2040S, Ipswich, MA) was used with the standard RNA synthesis protocol and incubated for 4 hours ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and lysed using the Tissue Lysis buffer from the Monarch® Genomic DNA Purification Kit (NEB, #T3010S) with double the volume of lysis buffer and a 1 h incubation at 56 C ...
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bioRxiv - Genomics 2023Quote: Strand-specific RNA libraries were generated using the NEBNext Ultra II stranded library kit (New England Biolabs) combined with polyA-coupled beads (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was purified for all samples using a Monarch RNA Cleanup Kit (New England Biolabs, T2040L), according to manufacturer’s recommendations ...
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bioRxiv - Synthetic Biology 2023Quote: ... Quick Blunting Kit and the T4 DNA Ligase enzyme were purchased from New England BioLabs (NEB, Germany). The plasmid extraction kit was purchased from Qiagen GmbH (Hilden ...
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bioRxiv - Neuroscience 2023Quote: ... and Cttn-VC155 were generated by NEBuilder® HiFi DNA Assembly Cloning Kit (# E5520S, New England BioLabs), which enables the assembly of multiple inserts within a linearized vector in a one-step PCR reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA seq libraries were constructed from total RNA using the NEBNext Ultra II Directional RNAseq kit (NEB) with ribosomal RNA depletion ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting gRNAs were purified using the AMPure XP kit and treated with DNase I (NEB, UK). Concentration was evaluated on a Qubit system (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and subsequently prepared for RNA-seq with the NEBNext Ultra Directional RNA Library Prep Kit (NEB #E7420) at GenomeScan ...
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bioRxiv - Microbiology 2023Quote: We performed RT-qPCR on select genes using the Luna Universal One-Step Kit (New England Biolabs) and QuantStudio 5 (Thermo) ...
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bioRxiv - Neuroscience 2023Quote: ... After quantification of individual libraries by q-PCR using the NEBNext library quant kit (New England BioLabs), all the samples were pooled in an equimolar manner ...
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bioRxiv - Genomics 2023Quote: Libraries were built on beads using the NEBNext Ultra II DNA Library Prep kit (NEB, Cat # E7645L). Biotin pull down was initiated py pipetting 20 μL Dynabeads My-One Streptavidin T1 beads (ThermoFisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... RNA library preparation was done using a NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) on a Bravo WS automation system (Agilent) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Sequencing libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Inc.) at Novogene and sequenced on an Illumina NovaSeq 6000 (2 × 150 bp ...
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bioRxiv - Genomics 2023Quote: ... An NGS library was generated using an NEB Ultra II DNA Library Prep kit (NEB, Ipswich, MA) with an Illumina compatible y-adaptor ...
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bioRxiv - Immunology 2023Quote: ... IVT was done using the HiScribe® T7 High-Yield RNA Synthesis Kit (New England Biolabs, #E2040S), with substitution of N-1-methyl-pseudouridine-5’-triphosphate (TriLink Biotechnologies ...
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bioRxiv - Genomics 2023Quote: ... Short-read libraries were prepared with the NEBNext Ultra II FS DNA Library Prep kit (NEB E7805), and sequenced 150 bp paired-end on an Illumina HiSeq3000 ...
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bioRxiv - Microbiology 2023Quote: cDNA was synthesized from extracted viral RNA using the LunaScript® RT SuperMix Kit (NEB, Ipswich, MA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... using poly(A) selection and the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). RNA-seq (2 × 100 nt paired-end reads ...
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bioRxiv - Genetics 2023Quote: ... 2 μg of total RNA was converted into cDNA with LunaScript RT SuperMix kit (New England Biolabs). The cDNAs were used as templates for real-time PCR and ran on StepOnePlus real-time PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... Colonies were prepared for whole genome sequencing using an Epicentre Masterpure DNA Preparation Kit (Nordic Biolabs, Sweden). Genomic DNA libraries were constructed using Nextera XT Library Preparation Kit (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... The library was quantified using the NEBNext® Library Quant Kit for Illumina® (New England Biolabs) by mixing 2 ul 1:10.000 diluted library with 8 ul Quant mastermix with added primers (New England Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted using the Monarch genomic DNA extraction and purification kit (New England Biolabs Inc.). Region of Cori and ter were quantitatively amplified using the Sybr green-based Luna Universal One-step RT-qPCR kit (New England Biolabs Inc.) ...
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bioRxiv - Microbiology 2023Quote: ... ChIP-sequencing libraries were generated using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) and sequenced with an Illumina HiSeq2500 (50 bp single-end) ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA library was prepared using the NEBnext Ultra II DNA Library Prep Kit (New England Biolabs, E7645) and NEBNext Multiplex Oligos for Illumina (NEB ...
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Regulation of transcription patterns, poly-ADP-ribose, and RNA-DNA hybrids by the ATM protein kinasebioRxiv - Molecular Biology 2023Quote: Total RNA extraction was performed using NEB Monarch Total RNA Miniprep Kit (New England Biolabs Cat #T2010S). RNAseq library prep was performed using NEBNext Ultra II RNA Library Prep for Illumina (NEB Cat# E7770S) ...
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Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted from overnight cultures using the Monarch Genomic DNA Purification Kit (New England Biolabs). DNA was quantified using a Qubit 4 fluorometer (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, Cat #E7645) following manufacture’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... NGS libraries were prepared using NEBnext Ultra II DNA library preparation kit for Illumina (New England Biolabs) and quantified with using a TapeStation (Agilent Genomics) ...
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bioRxiv - Cell Biology 2023Quote: ... Exon library was constructed using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB). DNA library hybridization was performed by using Agilent SureSelect Human All Exon V6 (Agilent) ...
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HOIL-1L deficiency induces cell cycle alteration which causes immaturity of myocyte and fibrogenesisbioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were prepared using a NEBNext Ultra II RNA Library Kit for Illumina (New England Biolabs) with the NEBNext Poly (A ...
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bioRxiv - Cell Biology 2023Quote: ... We created this deletion using a Q5 Site-Directed Mutagenesis kit (New England Biolabs, Cat. No: E0554S) with subsequent sequence verification ...
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bioRxiv - Genetics 2023Quote: ... 1x106 HEK293T cells were collected and their gDNA was extracted using the NEB Monarch kit (T3010S, NEB) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... 4NBX.B WT sequence with C103A mutation was created by site-directed mutagenesis using commercially available kits (NEB). Smt3 SUMO protein with an N-terminal cysteine was created from wild type Smt3 SUMO by mutagenic PCR ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was reverse transcribed from 1 µg of total RNA using LunaScript RT Supermix kit (NEB #E3010) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Isolated gDNA was then prepared for epigenetic sequencing using the Enzymatic Methylation Sequencing Kit (EM-Seq) (NEB).49 Individual embryos were sequenced at a depth of 100 million reads using a 2 × 150 bp Illumina kit on the Illumina NovaSeq instrument ...
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bioRxiv - Biophysics 2023Quote: ... Total RNA was extracted from cell cultures using the Monarch Total RNA Miniprep Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from extracted RNA samples using M-MuLV reverse transcriptase kit (New England Biolabs Ltd) based on the manufacturer’s instructions and using customized primers splice leader (SL_F ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was performed using the Luna® SARS-CoV-2 RT-qPCR Multiplex Assay Kit (NEB) with the CDC-derived primers for N1 and N2 gene targets and the reaction was performed using the QuantStudio™ 5 System (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... which was transcribed in vitro using the HiScribe T7 high-yield RNA synthesis kit (New England Biolabs). Afterwards ...
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bioRxiv - Microbiology 2023Quote: ... The sequencing library was constructed using an NEBNext rRNA Depeletion Kit (Bacteria) (New England Biolabs, Ipswich, MA). From the kit ...