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Citations for New England Biolabs :
401 - 450 of 5647 citations for Monoisononyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The reaction was mix using 1× CutSmart buffer (50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, and 100 μg/mL BSA; NEB) and 0.5 mM DTT (1,4-dithiothreitol ...
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bioRxiv - Genomics 2024Quote: ... Genomic DNA was harvested by discarding culture media and adding lysis buffer consisting of 20 mM Tris pH 8 and 0.1% Triton X-100 (MilliporeSigma T9284) with 60 ng/mL of Proteinase K (New England Biolabs P8107S) added immediately prior to use ...
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bioRxiv - Molecular Biology 2023Quote: ... using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer, NEB, 1 µL of 100 µM gapmer, 1 µL T4 PNK, NEB, 1 µL γ-32P-ATP, Hartmann, 6 µL ddH2O) for 40 min at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and NEBNext Multiplex Oligos for Illumina Primer sets 1 and 2 (New England Biolabs). The libraries were amplified with 7 PCR cycles using Herculase II Fusion Polymerase kit (Agilent) ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μL RT Adapter (RTA)(SQK-RNA002) and 2 μL T4 DNA Ligase (NEB) were mixed together and incubated under 25 °C for 10 min ...
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bioRxiv - Cancer Biology 2020Quote: ... and NEBNext Multiplex Oligos for Illumina (NEB Set 1 E7335 and Set 2 E7500S). Additional AMPure clean-ups at the start and the end of library preparation were included ...
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bioRxiv - Molecular Biology 2020Quote: ... Resulting PCR amplicons were denatured and re-annealed in 1 × NEB buffer 2 (NEB) in a total volume of 9 μl using a following conditions ...
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bioRxiv - Genomics 2023Quote: ... 20 of 2 U µL-1 Phusion High-Fidelity DNA Polymerase (New England Biolabs), 160 µL of 40 U µL-1 Taq DNA ligase (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... and chromatin was digested overnight by adding 25 µL 10X NEBuffer 2 and 100 U Mbo I (New England Biolabs, catalog No. R0147). Digestion efficiency was confirmed by isolating DNA from a 25-µL aliquot of the suspension and performing agarose gel electrophoresis ...
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bioRxiv - Genetics 2021Quote: Cells from each fin sample obtained as described before were washed in 2 ml PBS supplemented with 0.1% bovine serum albumin (BSA) (NEB). Each sample was divided into two replicate tubes ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 1.5 mg of protein was cleaved in a 2 ml reaction with 240 Units of TEV protease (NEB) for two hours at 30 °C ...
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bioRxiv - Physiology 2021Quote: ... with 2 mL freshly prepared TST buffer (0.03% Tween 20 [Bio-Rad], 0.01% Molecular Grade BSA [New England Biolabs] ...
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bioRxiv - Systems Biology 2020Quote: ... We discarded the supernatant and resuspended the pellet in 2 mL of 1x NEB buffer 3.1 (New England Biolabs).
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bioRxiv - Cancer Biology 2022Quote: ... The pellet was dissolved and digested in 50 ml buffer A with 2 mM CaCl2 and 4,000 units of micrococcal nuclease (M0247S, NEB) at RT for 20 min ...
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bioRxiv - Biochemistry 2021Quote: ... Heparinase digests were performed for 2 hours at 37 °C with a mixture of Heparinase I + II and III (3.5 mUnits/ml, NEB) in Heparinase digestion buffer (20 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μl of iTP_3’_linker_ApoI (10 μM) and 0.5 μl of ligase (T4 RNA ligase 2, truncated - 200 000 U/ml - New England Biolabs) per reaction ...
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bioRxiv - Microbiology 2024Quote: ... 3 ug RNA was treated with Promega RQ1 DNase (M6101) then cleaned up with the Monarch® RNA Cleanup Kit (NEB Biolabs, T2050S) and eluted in 30 µl dH2O.
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bioRxiv - Molecular Biology 2020Quote: ... Samples were ligated to barcode primers 1-20 of NEBNext Index Primers for Illumina sets 1 and 2 (New England Biolabs) and libraries analysed using DNA High Sensitivity chip on an Agilent 2100 Bioanalyzer before being pooled ...
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bioRxiv - Biophysics 2023Quote: ... and a reverse elongation primer (Supplementary Tables 1 and 2) and incubating for 1 cycle of annealing/extension with Q5 polymerase (New England Biolabs). dsDNA product was then incubated with ExoSAP-IT (Applied Biosystem ...
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bioRxiv - Bioengineering 2024Quote: ... containing 0.06 % pluronic acid (F-127, final concentration) and 1 µL of each disulfide bond enhancer 1 and 2 (NEB #E6820). We used droplet oil consisting of 3M™ Novec™ HFE7500 Engineered Fluid (3M ...
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bioRxiv - Microbiology 2023Quote: ... A 1 ml aliquot of each phage was treated with 1 µl DNase (New England Biolabs, USA) at 37 ⁰C for 40 minutes ...
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bioRxiv - Microbiology 2020Quote: ... 50µl of lysed cells were transferred to a tube containing 450µL of digestion mix (1X NEB 1 buffer, 1% triton X-100). This process was repeated 11 times (total 12 tubes with 500 µl) ...
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bioRxiv - Developmental Biology 2020Quote: ... the concentration of eluted MBP-mintbody was adjusted to 1 mg/ml and digested with 30 μg/ml Factor Xa (New England Biolabs) for 24 h ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μL of 2% SDS and 2 μL of proteinase K (New England Biolabs) were added and the solution was incubated at 37°C for 1 hour before purification with the Qiagen DNA clean up kit ...
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bioRxiv - Cancer Biology 2021Quote: ... or a polyclonal rabbit antibody against cleaved casp-3 (1:100; New England Biolabs, Frankfurt, Germany), followed by a biotinylated goat anti-rabbit secondary antibody (Abcam ...
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bioRxiv - Microbiology 2019Quote: ... 600 ng of total RNA were mixed with 0.83 μL 100 mM tris pH 7.5 and 0.17 μL 3 mg·mL−1 Random Primers (NEB) to a volume of 5.25 μL ...
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bioRxiv - Cell Biology 2022Quote: ... PV stock solution (1 mM) was prepared by adding 10 μl of 100 mM vanadate (NEB) and 50 μl of 100 mM hydrogen peroxide (diluted from a 30 % stock in 20 mM HEPES ...
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bioRxiv - Synthetic Biology 2020Quote: ... and immediately adding 1 U of β-agarase I per 100 ul of molten gel (NEB). The mixture was incubated at 42°C for 60 min to release DNA bound in the agarose matrix ...
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bioRxiv - Cell Biology 2023Quote: ... diluted 1:100 and used for the ligation using the Quick Ligation Kit (New England Biolabs). Specific base pair substitutions were introduced with site directed mutagenesis by polymerase chain reaction (PCR) ...
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bioRxiv - Microbiology 2021Quote: ... coli culture grown in LB supplemented with 100 μg/ml carbenicillin (LB+CA100) via miniprep kit (New England Biolabs, cat#T1010S). The plasmid was transformed into chemically competent E ...
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bioRxiv - Microbiology 2022Quote: ... the corresponding ORFs were amplified from the WT strain 536 and cloned into the pUC18 plasmid (ampicillin 100 μg/mL) using BamHI and SphI enzymes (New England Biolabs, USA) (ECP_3022 ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 μl 10X NEBuffer 2 (New England Biolabs) and nuclease-free water (total of 20 μl ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by adding 1 μL of proteinase K (800 units/ml) (NEB) and incubating at room temperature for 10 minutes before being run on a 1.7% agarose gel with a 100 bp ladder for 35 minutes.
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bioRxiv - Molecular Biology 2021Quote: ... and together with the addition of apyrase (25 mU mL−1, NEB). No CID2886111 was supplied in the purification ...
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bioRxiv - Microbiology 2020Quote: ... 0.8 mg ml-1 bovine serum albumin (BSA, New England Biolabs GmbH), 0.2 mM of each dNTP (Bioline ...
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bioRxiv - Genomics 2020Quote: ... The supernatant was loaded on four 1-ml chitin columns (NEB S6651S). Columns were washed with 10 ml HEGX ...
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bioRxiv - Microbiology 2021Quote: ... Immediately followed by enzymatic treatment of 1 mg/mL proteinase K (NEB) at 60°C for 60 min ...
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bioRxiv - Microbiology 2022Quote: ... 150 µl Proteinase K (800 units ml-1; New England Biolabs #P8107S), 138 µl 20% SDS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.3% Tween 20) with 1 μL proteinase K (800 U/ml, NEB) in 96 well plates ...
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bioRxiv - Synthetic Biology 2023Quote: ... Following one-hour outgrowth in 1 mL of SOC medium (NEB #B9020S) at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... The lysates were clarified and loaded on 1 mL chitin resin (NEB) and washed with 1 M KCl ...
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bioRxiv - Biochemistry 2023Quote: ... 1 M NaCl and 8 units/ml Proteinase K (New England Biolabs) in 1x PBS] ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were grown in 1 mL SOC Outgrowth Medium (B9020, NEB) for 2 h in a standard glass tube at 30 °C with 160 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... and reaction samples were loaded onto 1 ml of amylose resin (NEB). Proteins in the flow-through fractions were further purified by gel filtration chromatography (Superdex 200pg ...
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bioRxiv - Cancer Biology 2024Quote: ... prior to incubation with 1% SDS and proteinase K (20mg/mL, NEB) to inactivate and degrade pA-Tn5 ...
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Cis-regulatory divergence underpins the evolution of C3-C4 intermediate photosynthesis in MoricandiabioRxiv - Plant Biology 2021Quote: ... 17 μl total RNA (100 ng/μl) was added with 2 μl buffer and 0.5 μl RNase-free DNaseI enzyme (New England Biolabs GmbH, Frankfurt am Main, Germany) incubating on ice for 30 s ...
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bioRxiv - Genomics 2020Quote: ... Between 100 and 200 ng of genomic DNA was digested using 2 units of the MslI restriction enzyme (New England Biolabs ®, Ipswich, Massachusetts, USA.) and using 1 unit of NEB4 buffer in 20 μl of volume and for 2 hours at 37 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the resulting cell pellet was resuspended in 1 mL of RNA Protection Buffer (1× NEB DNA/RNA Protection Reagent, 1% (w/v) polyvinylpyrrolidone-40 ...
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bioRxiv - Synthetic Biology 2019Quote: ... 0.5 µl of 1 mg mL−1 purified bovine serum albumin (1:20 dilution in dH2O of BSA, Molecular Biology Grade 20 mg ml−1, NEB cat. B9000), 0.25 µl of T4 DNA ligase at 400 U µL−1 (NEB cat ...
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bioRxiv - Biochemistry 2020Quote: ... Beads were resuspended in 1 volume of buffer A / 300 mM NaCl / 2 mM MnCl2 / 1 mM DTT and incubated with λ protein phosphatase (NEB) at 50 U / mL for 1 hour at 23°C with agitation ...