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Citations for New England Biolabs :
401 - 450 of 1837 citations for Benzyl carbamic acid prop 2 ynyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... 2 μL of 10x Standard Taq Reaction Buffer (NEB), 0.4 μL of dNTPs (2.5 μM) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 units of DNase I (New England Biolabs, M0303S) were added ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µl PNGaseF (P0705L, Glycerol-free, New England Biolabs) was added and incubated for one hour at 57⁰C ...
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bioRxiv - Bioengineering 2023Quote: ... and mRNA Cap 2’-O-methyltransferase (NEB, catalog #M0366S). We then added a 3′-poly(A ...
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bioRxiv - Genomics 2022Quote: ... and 2 mg/mL proteinase K (New England Biolabs). The tissue sections were lysed separately in 75 μL lysis buffer for 2 hours at 55 °C ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 mM of rNTPs (New England Biolabs GmbH (Germany)) ...
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bioRxiv - Cell Biology 2022Quote: ... pellets were resuspended with 1.11× NEBuffer 2 (B7002, NEB) containing 0.1% sodium dodecyl sulfate (SDS) ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 µl of T4 DNA Ligase Buffer (NEB M0202), 1 µl of Restriction Enzyme (20 U ...
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bioRxiv - Biochemistry 2023Quote: ... 2 uL dNTP Solution Mix (10 mM stock, NEB), 2 μl Taq DNA ligase (80 U ...
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bioRxiv - Plant Biology 2023Quote: ... 2 and T4 DNA ligase (both New England Biolabs).
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bioRxiv - Genomics 2023Quote: ... and digested with 2 units of β-agarase (NEB) per block (1 hr. ...
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bioRxiv - Biochemistry 2023Quote: ... SNAP-Cell TMR Star (2 mM in DMSO, NEB), and SNAP-Cell Block (2 mM in DMSO ...
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bioRxiv - Biochemistry 2023Quote: ... and SNAP-Cell Block (2 mM in DMSO, NEB) were prepared and stored as aliquots in the dark at –20 °C ...
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bioRxiv - Genomics 2023Quote: ... and digested with 2 units of β-agarase (NEB) (42°C ...
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bioRxiv - Microbiology 2024Quote: ... 200 U T4 RNA ligase 2 truncated KQ (NEB), 1x T4 RNA ligase buffer (NEB) ...
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bioRxiv - Genomics 2024Quote: ... 2 mg/mL temperature labile proteinase K (NEB, P8111S), and 12.5 mM MgCl2 for RNA fragmentation) ...
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bioRxiv - Biochemistry 2024Quote: ... using Q5® High-Fidelity 2× Master Mix (NEB) and 200 nM of each primer ...
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bioRxiv - Cancer Biology 2024Quote: ... 25 µl NEBNext HiFi 2× PCR Master mix (NEB) was immediately added and mixed before proceeding to PCR cycles (58 °C for 5 min (gap filling) ...
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bioRxiv - Biochemistry 2024Quote: ... and 2 U of Phusion polymerase (New England Biolabs). Reactions were initially denatured at 98 °C for 30 s ...
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bioRxiv - Genomics 2024Quote: ... and 25 μL 10xNEB buffer 2 (NEB B7002 RT) were added to the reaction and incubated at 37°C for 2 hours with rotation ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µL 10× T4 RNA Ligase Reaction Buffer (NEB), 6 µL PEG8000 (50%) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 2 µl of DNase I (2500U/mL, NEB) for each mL of B-PER reagent used ...
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bioRxiv - Synthetic Biology 2024Quote: ... T4 RNA Ligase 2 (10U/µl New England Biolabs) and were incubated at 37°C for 2 h ...
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bioRxiv - Molecular Biology 2024Quote: ... Residual RNA was digested with 2 units RNaseH (NEB) for 20 min at 37°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 2 units of polymerase (New England Biolabs M0254). Vent exo– (New England Biolabs ...
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bioRxiv - Synthetic Biology 2024Quote: ... 25 µL of 2× Q5 Master Mix (NEB, UK) enzyme mix ...
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bioRxiv - Genetics 2024Quote: ... and 2 mM Ribonucleoside Vanadyl complex (New England Biolabs). Coverslips were preserved for later use in 70% EtOH at -20°C ...
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bioRxiv - Bioengineering 2024Quote: ... 2 μL of BsaI-HFv2 (NEB, 20000 U/mL), 2 μL of T4 DNA ligase (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... The digestion experiments used 2% AluI or MNase (NEB), 10% NEB CutSmart buffer or MNase buffer ...
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bioRxiv - Microbiology 2020Quote: ... amplified with the primers Dhm1275-1 and −2 (Table 2) using a NEBuilder HiFi DNA Assembly Kit (New England BioLabs, Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... 5 μl of 100 ng/μl cDNA diluted from the previous step was combined with 2 μl block mix and 2 μl nuclease free water (NEB, catalog no. AM9937), then the cDNA-block oligo mix was incubated on a thermocycler under the following condition to allow block oligo mix to bind to 5’ and 3’ end of the cDNA molecule ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µL ligation mix (2 µL 10x T4 ligase buffer, NEB, 1 µL T4 RNA ligase 2, truncated, NEB, 1 µL Ribolock inhibitor) was added and incubated (1 h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was prepared by combining 2 µL of 100 µM guide RNA (gRNA) with 2 µL of 20 µM EnGen SpyCas9 NLS (NEB, Cat. No. M0646T), and incubated at room temperature for 2-4 hours ...
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bioRxiv - Biochemistry 2022Quote: ... the corresponding single and multiple amino acid substitutions were introduced by QuikChange site-directed mutagenesis (NEB, Phusion High-Fidelity PCR Kit) followed by KLD (Kinase ...
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bioRxiv - Neuroscience 2020Quote: ... amino acid sequence identical to GenBank: BC002453.2) was amplified using Q5 High-Fidelity DNA Polymerase (New England Biolabs, Ipswich MA, USA) and inserted into the TOPO cloning site in pET151/D-TOPO E ...
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bioRxiv - Molecular Biology 2021Quote: ... or more amino acid substitutions in SecPH with Q5 PCR methodology (Q5®Site-Directed Mutagenesis Kit, New England Biolabs, E0554) or PCR site-directed mutagenesis strategy ...
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bioRxiv - Immunology 2021Quote: ... were generated by introducing the corresponding amino acid mutations (Extended Data Fig. 5) using the Q5® Site-Directed Mutagenesis Kit (NEB) and per manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... The OprCAA mutant was produced by changing the key amino acids Cys143 and Met147 to alanine residues using the KLD Quickchange site-directed mutagenesis kit (New England Biolabs, UK) and specific primers containing both mutation sites (forward ...
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bioRxiv - Cell Biology 2022Quote: ... 6 μg of digested nucleic acids were treated overnight at 37 °C with or without 10 μl of RNase H (New England BioLabs, M029L) in 1x RNase H buffer and 1/10th of the samples was saved as input ...
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bioRxiv - Molecular Biology 2024Quote: ... STK19 was generated by deleting the first 110 amino acids of the misannotated STK19 using Q5 site directed mutagenesis (NEB, E0554S) and addition of GFP was done using In-Fusion system (Takara 639649).
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bioRxiv - Biochemistry 2024Quote: ... A truncated version of this wild-type VgrS lacking the first 81 amino acids was created by Q5-mutagenesis (NewEngland Biolabs, NEB) to generate VgrS (residues 81-729 ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 µL of linker ligation mixture was added (38% PEG-8000, 1x NEB T4 ligase buffer, 2 µM oBZ407_preA preadenylated linker, 100 units NEB T4 RNA ligase 2, truncated) and they were further incubated at 37 °C for 3 hrs ...
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bioRxiv - Microbiology 2020Quote: ... then quenched with 2 μL 6X purple loading dye (NEB) at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 µL of 10x uracil DNA glycosylase buffer (NEB M0280S), 2.5 µL RNase A (M0280S ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 units of 2’-O-Me-transferase (New England Biolabs) and 25 units RNasin Plus RNase inhibitor (Promega) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 200 U/uL of T4 RNA Ligase 2 (NEB M0351S), 1X T4 RNA Ligase Reaction Buffer (NEB B0216L) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 2 uL of 10X T4 RNA ligation buffer (NEB B0216L), 2 uL of 10mM ATP ...
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bioRxiv - Developmental Biology 2020Quote: ... a mix containing 2 μl of RNAse H (NEB, #M0297S), 1 μl of E ...
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bioRxiv - Genomics 2020Quote: ... 2 μL of NEB buffer 3.1(1X, New England Biolabs) and 1 μL of each DNA barcode B and ligation linker mix (B1-B50 ...
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bioRxiv - Microbiology 2022Quote: ... PEG 8000 (17.5% final) and T4 RNA ligase 2 (NEB) in 1x T4 RNA ligase buffer at 25°C for 1 h ...