Labshake search
Citations for New England Biolabs :
4351 - 4400 of 9451 citations for Human Zyxin ZYX ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Isolation of plasmid DNA was performed with a Monarch Plasmid Miniprep Kit/T1010 (New England Biolabs, UK). The concentration and purity of DNA preparations were determined using a Nanodrop 2000 instrument (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted from flies/tissues with the Monarch® Genomic DNA Purification Kit (New England Biolabs). Strain specific abundance was assessed with the Luna® Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted from individual flies with the Monarch® Genomic DNA Purification Kit (New England Biolabs), PCR assays were performed with the strain-specific multiplex primers from (46 ...
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bioRxiv - Immunology 2022Quote: ... Excess oligos and dNTPs were removed from amplified cDNA with EXO-CIP cleanup kit (New England BioLabs).
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bioRxiv - Microbiology 2022Quote: ... RNA was treated with DNase and synthesized using a ProtoScript II First Strand cDNA Synthesis Kit (NEB). Primers used in PCR amplification are ...
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bioRxiv - Microbiology 2022Quote: ... and ligated into plentiCRISPRv2 using NEB Golden Gate Assembly Kit (BsmBI-v2) (New England Biolabs Inc. # E1602S). The ligation reaction was dialyzed on a Type-VS Millipore membrane and electroporated into Endura electrocompetent cells ...
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bioRxiv - Microbiology 2022Quote: The translatability of hlgCB and hlgB mRNAs were assessed using the PURExpress® Δ Ribosome Kit (NEB) supplemented with purified S ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids and amplicons were purified from agarose gel using Monarch Gel purification kit (New England Biolabs®) and ligated using T4 ligase from New England Biolabs® ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using the Luna universal one-step RT-qPCR kit (#E3005, New England Biolabs) according to the provided protocol ...
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bioRxiv - Microbiology 2022Quote: ... cDNA were synthesised from 1 µg RNA by reverse transcription using LunaScript RT SuperMix Kit (NEB; E3010). qRT-PCR was performed using Luna Universal qPCR Master Mix (NEB ...
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bioRxiv - Plant Biology 2022Quote: ... using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were prepared using the NEBNext Ultra II Library Prep Kit for Illumina (New England Biolabs, E7645) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The final pool consisting of 797 sgRNAs was purified using the Monarch RNA Cleanup Kit (500ug, NEB). sgRNA quality control was performed on Qubit and Bioanalyzer RNA 6000 Pico Kit (Agilent).
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bioRxiv - Neuroscience 2023Quote: ... the cDNA libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) and were assessed using Qubit 2.0 fluorometer to calculate the concentrations and Bioanalyzer Instrument to determine insert size ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Microbiology 2023Quote: ... Primer pairs M01/M02 and M03/M04 were used with the Q5 site-directed mutagenesis kit (NEB) to introduce point mutations for the T161V and D296N substitutions ...
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bioRxiv - Physiology 2023Quote: ... individuals from each species using the Monarch total RNA Miniprep Kit (T2010S, New England Biolabs, Ipswich, MA) following manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... the RNA was reverse transcribed using the LunaScript RT Supermix Kit (New England BioLabs, NEB; Ipswich, MA). Tiled 1200 bp amplicons were generated using midnight primers (IDT ...
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bioRxiv - Microbiology 2022Quote: ... the RNA was reverse transcribed using the LunaScript RT Supermix Kit (New England BioLabs, NEB; Ipswich, MA). Tiled 1200 bp amplicons were generated using midnight primers (IDT ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Genomic libraries were generated using the NEBNext Ultra II FS kit (New England Biolabs, Ipswich, Massachusetts, USA) with sonication in an M220 Focused-ultrasonicator (Covaris ...
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bioRxiv - Genetics 2022Quote: ... end prep and ligation) was performed using the NEB Ultra II RNA custom kit (New England Biolabs) on an Agilent Bravo automated system ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, #E7530L). All libraries were prepared using manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA sequencing library preparation was performed using the NEBNext® DNA Library Prep Kit (New England Biolabs) following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2022Quote: ... We then consecutively mutated the Y549A mutation using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) with primer set ...
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bioRxiv - Synthetic Biology 2022Quote: ... The S222N mutant of AtADT212 was recreated by site-directed mutagenesis (Q5® Kit, NEB, Ipswich, MA). The empty vector was created by PCR-amplifying the pHyo182 backbone outside the start and stop codons of the AtADT2 ORF and ligating the ends ...
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bioRxiv - Molecular Biology 2022Quote: ... total RNA from U2OS cells were extracted by using Monarch Total RNA Miniprep kit (New England Biolabs) and treated with TURBO DNase (Thermo Fisher) ...
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bioRxiv - Plant Biology 2022Quote: ... the sequencing libraries were constructed with a NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) and sequenced on the HiSeq 4000 platform to obtain 150-bp paired-end data ...
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bioRxiv - Microbiology 2022Quote: ... the cDNA libraries were generated using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, USA) following manufacture’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared with the NEBNext® Ultra II DNA Library Prep Kit for Illumina (NEB, E7645L) and single-end (1 x 75 bp ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and performing directed mutagenesis for each TFBS with the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Primers used for deleting the TFBSs can be found in Table S6 ...
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bioRxiv - Immunology 2022Quote: ... Fragments were cloned into HpaI-digested pMSCVpuro using the Gibson Assembly Cloning Kit (New England Biolabs, E5510S). Plasmids were transformed into E ...
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bioRxiv - Synthetic Biology 2022Quote: ... whole genome sequencing libraries were generated using the NEBNext Ultra II FS DNA Library Prep Kit (NEB) and sequenced using an Illumina NextSeq 500/550 High Output Kit v2.5 (75 Cycles) ...
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bioRxiv - Cell Biology 2022Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... and used for in-vitro transcription using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). Finally ...
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bioRxiv - Microbiology 2024Quote: ... DNA libraries were constructed using NEBNext® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... A repair template plasmid was constructed using Gibson assemble (Gibson et al., 2008) (NEB Hifi assembly kit). The repair construct was made by joining the PfAMA1 coding region and ∼1.5 kb 5’ to the transcription start site amplified from 3D7 genomic DNA ...
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bioRxiv - Microbiology 2024Quote: ... libraries were prepared using the NEBNext® Ultra™ Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and library prep were performed in parallel using the Ultra II Directional RNA-Seq Kit (NEB E7760) and reads were sequenced 2x50bp on an Illumina NovaSeq S4.
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bioRxiv - Biochemistry 2024Quote: ... The inserts were each ligated with digested pmirGLO plasmids using a quick ligasation kit (New England Biolabs) and transformed into JM109 competent cells (Promega) ...
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bioRxiv - Genomics 2023Quote: ... DNase-seq libraries were prepared using the NEBNext Ultra Library Prep Kit for Illumina (New England Biolabs). Illumina sequencing was performed on an Illumina HiSeq instrument to a depth of 12 to 33 million mapped 40-50 bp single-end sequence reads per sample ...
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bioRxiv - Microbiology 2023Quote: ... and used to prepare the library with the Next ultra II DNA library prep kit (E7645, NEB) using 1 μg of DNA per sample as input ...
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bioRxiv - Biochemistry 2024Quote: ... Fragments were assembled into the pBAD24 vector using HiFi DNA Assembly Kit (New England Biolabs, Ipswich, MA) with 1-hour ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was isolated from ∼2×106 cells using the Monarch DNA purification Kit (New England Biolabs). For genotyping ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmids were then isolated using the Monarch® Plasmid Miniprep Kit (New England Biolabs, Ipswich, MA, USA) and used to transfect HEK293T cells (ECACC ...
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bioRxiv - Cell Biology 2024Quote: ... The ChIP libraries were constructed utilizing NEBNext® Ultra™ II DNA Library Prep Kit (NEB, E7645L) and sequenced on an Illumina NovaSeq 6000 PE150 platform using the 150-bp pair-end sequencing parameters (Novogene ...
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bioRxiv - Cell Biology 2024Quote: ... RNA libraries were constructed utilizing the NEBNext® Ultra RNA Library Prep Kit for Illumina (NEB, E7530L) and sequenced on an Illumina NovaSeq 6000 PE150 platform using the 150-bp pair-end sequencing parameters (Novogene ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 uL RNase A and 1 uL ProtK all from the Monarch gDNA extraction kit (NEB, T3010). gDNA was extracted following the extraction kit’s protocol with the exception that 600 uL of gDNA binding buffer were added to 400 uL quenched reaction and added to the extraction column on two spins of 2 minutes at 1000g ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was isolated from the cells using Monarch® Genomic DNA Purification Kit (New England Biolabs). DNA concentration was measured with Nanodrop One/OneC spectrophotometer (Thermo Fisher) ...