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Citations for New England Biolabs :
4201 - 4250 of 9366 citations for Rat Alanine Glyoxylate Aminotransferase AGXT ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... crRNA was synthesized in vitro using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) by incubating the reaction at 37°C with T7 promoter primer (10μM ...
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bioRxiv - Molecular Biology 2021Quote: ... all ChIP-DNA libraries were produced using the NEBNext Ultra II DNA Library Prep Kit (NEB, E7645L) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... and in vitro synthesized RNA was subsequently purified using Monarch RNA Cleanup Kit (Cat. No. T2040S, NEB). RNA concentration was quantified by NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... followed by library preparation with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Library preparation included 11 cycles of PCR amplification ...
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bioRxiv - Microbiology 2021Quote: ... SERBP1 mutant and Vigilin mutant were obtained using the Q5® Site-Directed Mutagenesis Kit (E0554) (NEB) with deletion primers using the WT cDNA in pLVX as template ...
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bioRxiv - Immunology 2020Quote: ... (2) and (3) was synthesized through HiScribe™ T7 ARCA mRNA Kit (with tailing) (New England Biolabs). All the mRNA products were concentrated and purified via EZ-10 Spin Column RNA Cleanup & Concentration Kit (Bio Basic Inc.) ...
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bioRxiv - Microbiology 2021Quote: ... were Deletions and point mutations in HelR were created using the Q5 site directed mutagenesis kit (NEB). Primers for mutant construction were designed using the NEBaseChanger as recommended ...
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bioRxiv - Microbiology 2021Quote: ... The DNA samples were pooled into two libraries prepared with the NEBNext Ultra kit (New England BioLabs). They were sequenced on a MiSeq sequencer (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... DNA fragments were amplified by PCR and assembled by NEBuilder HiFi DNA Assembly Cloning Kit (NEB, E5520S).
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bioRxiv - Genetics 2020Quote: ... RNA was reverse transcribed to cDNA using the ProtoScript II First Strand synthesis kit (NEB, Cat# E6560S) and synthesis was performed with the “random primer” option included within the kit ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Genomics 2021Quote: ... we purified the genomic DNA from each timepoint with the Monarch gDNA extraction Kit (New England Biolabs). We then amplified the region of one of the mutations acquired by the Tf-null strain (I:1453108 C->A ...
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bioRxiv - Genomics 2021Quote: ... gDNA was extracted with the Monarch Genomic DNA purification kit (#T3010 New England Biolabs; Ipswich, MA, USA). For Oxford Nanopore sequencing of STK005 ...
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries were produced using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB). Libraries were sequenced on Illumina HiSeq platform.
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bioRxiv - Genomics 2021Quote: ... Standard RNA Synthesis was carried out with the HiScribe T7 High Yield RNA Synthesis kit (NEB E2040S) for 1.5 hours according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Libraries were prepared for high-throughput sequencing using the Ultra II DNA Library Prep kit (NEB E7645L). For IP samples ...
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bioRxiv - Genomics 2020Quote: ... Plasmid libraries were harvested from liquid culture using the Monarch® Plasmid DNA Miniprep Kit (NEB #T1010). Miniprep kit reagents were scaled to accommodate one spin column for every 5mL of culture.
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bioRxiv - Microbiology 2021Quote: ... Strand-specific RNA-seq was performed using the NEBNext Ultra Directional RNA Library Prep Kit (NEB, E7420) with the following adjustments ...
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bioRxiv - Biochemistry 2020Quote: ... TSEN2H377A and TSEN34H255A point mutants were generated using the Q5 site-directed mutagenesis kit (New England Biolabs) prior to assembly into biGBac vectors ...
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bioRxiv - Cancer Biology 2021Quote: Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations with index codes added to attribute sequences to each sample ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We prepared cDNA libraries with the NEBNext Ultra RNA Library Prep Kit for Illumina (NewEngland Biolabs, MA) with poly(A ...
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bioRxiv - Molecular Biology 2021Quote: ... Non-binding mutant (F212A) was generated from template MHV-S_HE_Fc_Avitag using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs)
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bioRxiv - Microbiology 2022Quote: ... DNAse treatment was performed as described above and cDNA was synthesized using LunaScript RT Supermix Kit (NEB). RT and non-RT vials were checked by PCR with primers 7,8 for PgV (Supplementary File 2) ...
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bioRxiv - Microbiology 2022Quote: ... 0.5 µL of dNTPs (HiScribe™ T7 Quick High Yield RNA Synthesis Kit, NEB, Ipswich, MA, USA), 0.5 µL of T7 polymerase (HiScribe™ T7 Quick High Yield RNA Synthesis Kit ...
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bioRxiv - Developmental Biology 2022Quote: ... end prep and ligation) was performed using the ‘NEB Ultra II RNA custom kit’ (New England Biolabs) on an Agilent Bravo WS automation system ...
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bioRxiv - Genetics 2022Quote: ... poly-A RNA libraries were generated using (NEBNext Ultra Directional RNA Library Prep kit, New England Biolabs, Ipswich ...
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bioRxiv - Cell Biology 2022Quote: ... Mutants of IR and IGF1R were generated by Q5 site-directed mutagenesis kit (New England BioLabs Inc.). Plasmid transfections into 293FT cells were performed with LipofectaminTM 2000 (Invitrogen) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The two PCR fragments were then assembled using the HiFi Gibson Assembly kit from NEB (Waltham, MA) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... Non-fluorescent RNA was transcribed using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, #E2040S). Following in vitro transcription by either kit ...
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bioRxiv - Molecular Biology 2022Quote: ... Indexed sequencing libraries were generated using the NEBNext Ultra II DNA Library Prep kit (NEB Cat # E7645), pooled ...
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bioRxiv - Molecular Biology 2022Quote: DNA libraries for deep sequencing were made using the NEBNext Ultra II DNA library preparation kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... fragmented DNA was processed using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Library preparation was modified from protocols.IO (dx.doi.org/10.17504/protocols.io.bagaibse) utilizing reagents from the NEB Next II DNA Ultra Kit (New England Biolabs #E7645S) and Unique Combinatorial Dual index kit (New England Biolabs #E6442S, ...
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bioRxiv - Biophysics 2022Quote: ... sgRNA was produced by in vitro transcription using the HiScribe T7 High Yield RNA synthesis kit (NEB) with the PCR amplified DNA sequence as the template ...
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bioRxiv - Biochemistry 2022Quote: ... products corresponding to the correct size were excised and purified using Monarch DNA Gel Extraction kit (NEB). For the second round of PCR ...
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bioRxiv - Biochemistry 2022Quote: ... PCR products corresponding to the correct size were excised and purified using DNA Gel Extraction kit (NEB). 100 ng of each gel-purified PCR products (total of 19 ...
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bioRxiv - Biochemistry 2022Quote: The amplicons described above were purified with the Monarch PCR and DNA purification kit (New England Biolabs) and sent for NGS EZ Amplicon Sequencing by Genewiz ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Biochemistry 2022Quote: ... Polyadenylated transcript enrichment and strand specific library preparation was completed using NEBNext Ultra II mRNA kit (NEB) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA was purified by standard methods and rRNA depleted using the NEBNext rRNA depletion kit (NEB, as per the manufacturer’s instructions) ...
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bioRxiv - Developmental Biology 2022Quote: ... the library was prepared using the NEBNext Ultra RNA library prep kit for Illumina (New England Biolabs). Sequences with 150 bp read length × 2 paired ends and 6 Gb of data were obtained using the next-generation sequencer ...
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bioRxiv - Developmental Biology 2022Quote: ... Strand-specific RNA-seq libraries were constructed using NEB Ultra II RNA-seq library kit (NEB #E7765) according to manufacturer’s protocol with fragmentation in first-strand buffer at 94°C for 15 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Eluted DNA was enriched with PCR reaction using Fusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Cell Biology 2022Quote: ... Both products were purified and cloned pBTM116 using the NEBuilder HiFi DNA assembly kit (New England Biolabs).
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bioRxiv - Cell Biology 2022Quote: ... that were cloned into pFA6a-3V5-KanMX6 using the NEBuilder HiFi DNA Assembly kit (New England Biolabs). Strains for pairwise competition assays were generated by inserting a Hyg-TEFp-GFP expression cassette into a gene-free region of chromosome VI42 ...
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bioRxiv - Immunology 2022Quote: ... DNA libraries were built using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NewEngland Biolabs) with the following modifications.83 NEBNext End Prep step was performed using 25μl of input material for a final volume of 30μl and the following adapted program (30min-20°C ...
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bioRxiv - Genomics 2022Quote: ... RNA was isolated and cleaned up using the Monarch Total RNA Miniprep Kit (New England BioLabs, T2010S). RNA quality and concentration were assessed using a Nanodrop photospectrometer ND-1000 (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... The PCR product was cleaned using the Monarch® PCR clean up kit (New England Biolabs T1030S) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: The TF samples were expressed by using a PURExpress In Vitro Protein Synthesis Kit (New England BioLabs) and analyzed in duplicate on two different PBM arrays (HK and ME ...
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bioRxiv - Developmental Biology 2022Quote: ... The sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) following the manufacturer’s instructions ...