Labshake search
Citations for New England Biolabs :
4101 - 4150 of 9547 citations for QuantiChrom Phosphate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... CPB containing a C-terminal His6-tag was generated using Q5® site-directed mutagenesis kit (NEB), pET-19b_His6_CPB as template and primer pair #1 and elongation time of 4 min to delete the N-terminal His6 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #7760), with cDNA generated from fresh RNA samples using 500ng of RNA and 10 cycles of PCR ...
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bioRxiv - Molecular Biology 2020Quote: Purified DNA or cDNA was subjected to NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). The DNA was resuspended in 25 μl ddH2O ...
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bioRxiv - Molecular Biology 2021Quote: ... This is then followed by second-strand synthesis and NEB ultra-directional RNA sequencing kit (NEB #E7760).
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bioRxiv - Molecular Biology 2021Quote: sgRNAs were produced by in vitro transcription using the HiScribe T7 High Yield RNA synthesis kit (NEB) with PCR amplified gBlocks (IDT ...
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bioRxiv - Molecular Biology 2020Quote: Libraries were prepared for sequencing using the NEBNext Ultra II library preparation kit (New England Biolabs, USA) according to manufacturer’s protocol and barcoded using NEXTflex barcodes (BIOO Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... pGEMHE plasmids were linearized and 5’-capped mRNA was synthesised with T7 polymerase (NEB HiScribeT7 ARCA kit) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ChIP-Seq libraries were constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB E7645) and barcoded multiplex libraries were sequenced using Illumina MiSeq platform ...
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bioRxiv - Molecular Biology 2021Quote: ... Mutations were introduced into UNC-45B wild-type using the Q5® Site-Directed Mutagenesis Kit (NEB). Recombinant protein expression was induced in BL21 DE3 when optical density (OD600 ...
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bioRxiv - Genomics 2022Quote: ... and a library was prepared using the NEBNext Ultra II DNA library prep kit for Illumina (NEB) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Library preparation was conducted using an NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, Ipswich, MA), with a 12-cycle PCR enrichment ...
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bioRxiv - Genomics 2020Quote: ... Quantification of libraries was done by qPCR using the NEBNext library quant kit for Illumina (NEB, E7630) and library sizes were analyzed using the Agilent bioanalyzer 2100 HS DNA kit ...
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bioRxiv - Genomics 2020Quote: ... DNA was end-repaired using the NEBNext Ultra II End Repair/dA Tailing kit (New England Biolabs) and purified using AMPure XP beads ...
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bioRxiv - Genomics 2019Quote: ... Sequencing library preparation was performed using the NEBNext Ultra DNA library prep kit for Illumina (NEB, E7370S), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Libraries were generated using the NEBNext Ultra II Directional Library Prep Kit (New England Biolabs, Ipswich, MA) and subjected to sequencing (single-end 92x ...
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bioRxiv - Genomics 2020Quote: ... libraries were constructed with the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Illumina DNA sequencing libraries were prepared using the NEBNext Ultra II library preparation kit (New England Biolabs). 2 × 250 bp paired end sequencing reads were obtained using an Illumina HiSeq 2500 ...
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bioRxiv - Genomics 2020Quote: ... Libraries for Illumina sequencing were prepared following the NEBNext DNA Library Prep Master Mix kit (NEB E6040). Amplified libraries were size selected using a 2% agarose gel to extract fragments between 200 and 600 bp.
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bioRxiv - Genomics 2020Quote: ... RNA-seq libraries were generated using the NEBNext Ultra Directional RNA Library Prep kit for Illumina (NEB). Agilent DNA High Sensitivity Chips (Agilent Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep kit (New England Biolabs). Samples were indexed using NEBNext Multiplex Oligos (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... and then further purified using the Monarch Total RNA Miniprep Kit (New England Biolabs, Ipswich, MA, USA). A total of 250 ng RNA was used to synthesize cDNA with Reverse Tra Ace qPCR RT Master Mix (Toyobo Co. ...
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bioRxiv - Microbiology 2019Quote: ... we performed q-RT PCR using the Luna Universal One-Step RT-qPCR Kit from NEB (E3005), using the Applied BiosystemsΤΜ 7500 Real-Time PCR system ...
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bioRxiv - Developmental Biology 2020Quote: sgRNAs were then transcribed from templates using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) and purified using Megaclear Kit (Life Technologies) ...
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bioRxiv - Microbiology 2019Quote: ... The gltA complement plasmid was constructed using a Gibson Assembly Cloning Kit (New England Biolabs, Ipswich, MA). Briefly ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and whole genome sequencing libraries were prepared using the NEBNext Ultra II Library kit (New England Biolabs) according the recommended protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Transcriptomic sequencing of RNA was performed using NEBNext® rRNA Depletion Kit (New England Biolabs, Inc.; E6310S) and SMARTer Stranded Total RNA-Seq Kit - Pico Input (Clontech ...
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bioRxiv - Bioengineering 2019Quote: ... The NsiI site was then removed using the Q5 Site-directed mutagenesis Kit (New England Biolabs, E0554S). All sgRNA sequences for activation studies (Supplementary Table 1a ...
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bioRxiv - Genetics 2020Quote: ... and the library generated with a NEB Next Ultra II DNA Library Prep Kit for Illumina (NEB). The quality of the library was checked using an Agilent 2100 Bioanalyzer (Agilent High Sensitivity DNA kit) ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted DNA fragments were purified on a Monarch PCR & DNA Cleanup Kit (#T1030S, New England BioLabs and eluted with 26 μL DNAase and RNAase free water ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: WGS was performed using a modified NEBNext UltraII for DNA Library Prep kit (E7645L, New England BioLabs). Briefly ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... We cloned the gBlock insert into our vector library using the Quick Ligation Kit (New England BioLabs) and transformed and prepared DNA from this plasmid library as detailed above ...
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bioRxiv - Genetics 2019Quote: ... and used as input into the NEB Ultra II Directional RNA library kit for Illumina (NEB E7760) in two batches ...
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bioRxiv - Genetics 2019Quote: ... Sequencing libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). The mRNA was selected by purifying polyA+ transcripts (NEBNext Poly(A ...
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bioRxiv - Biochemistry 2019Quote: ... Double stranded RNA reactions were performed using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, #E2040S) and purified with the GeneJET RNA Purification Kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and a library was prepared using the NEBNext Ultra DNA library preparation kit (catalog number E7370L; NEB). Whole-genome sequencing of 150bp paired-end reads was generated on Illumina HiSeq2500 machine to a target coverage of 20X per sample ...
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bioRxiv - Microbiology 2019Quote: KLRC2 genotypes were determined by touchdown PCR using the Phusion High Fidelity PCR kit (New England Biolabs) using previously described methods40 ...
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bioRxiv - Genomics 2019Quote: ... Purified DNA sample was end-repaired (NEBnext ultra II end repair kit, New England Biolabs, MA, USA), cleaned up with 1x AMPure XP beads (Beckmann Coulter ...
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bioRxiv - Microbiology 2019Quote: Variants of UbiV and UbiU were constructed using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) according to the manufacturer’s specifications ...
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bioRxiv - Genomics 2019Quote: ... The second experimental replicate was prepared using the NEBNext Ultra II kit (New England Biolabs, Hitchin, UK) and sequenced using an Illumina NextSeq 500 platform ...
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bioRxiv - Plant Biology 2019Quote: ... ZymoResearch) and reverse-transcribed in the presence of random hexamers (LunaScript®RT SuperMix Kit, NEB Inc.) in 20 μl of total reaction volume containing 1 μg of total RNA and incubated at 55°C for 15 minutes following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 2013) with probes synthesized using components based on the NEBlot Phototope Kit (New England BioLabs, Cat# N7550) and detected using components based on the Phototope-Star detection kit (New England BioLabs ...
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bioRxiv - Evolutionary Biology 2019Quote: ... RNA sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #E7530). NEBNext Poly(A ...
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bioRxiv - Microbiology 2019Quote: ... The in vitro transcripts were produced using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Libraries were then cloned using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB), following the recommended kit protocol and sequenced on a HiSeq2500 (Illumina).
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bioRxiv - Biochemistry 2020Quote: DNA was extracted from HLCs using the Monarch® Genomic DNA Purification Kit (New England BioLabs, T3010). A master mix was prepared using the Luna® Universal qPCR Master Mix (New England BioLabs ...
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bioRxiv - Molecular Biology 2021Quote: ... and final RNA-seq libraries generated using the NEBNext Ultra II Directional RNA Library Prep kit (NEB, E7760 and NEBNext Multiplex Oligos (NEB ...
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bioRxiv - Genomics 2019Quote: ... Fragmented DNA was used for library preparation using a NEBNext Ultra II Kit (NEB, Ipswich, MA USA) according to the manufacturer’s instructions for DNA end repair ...
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bioRxiv - Genetics 2021Quote: ... cDNA library was prepared with NEBNext Ultra II RNA library prep kit for Illumina (New England Biolabs), and the sequencing carried out using Illumina’s HiSeq-2500 sequencer with single-end mode and read length of 50 bp ...
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bioRxiv - Genetics 2020Quote: ... RNA was then reverse transcribed to cDNA using LunaScript RT SuperMix kit (Catalog # E3010L, New England BioLabs). w1118 flies might have partial or truncated transcripts of w+ ...
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bioRxiv - Genetics 2020Quote: ... Variants were generated using three-way Gibson cloning using NEB HiFi DNA Assembly Cloning Kit (NEB, E5520), using a NotI-AscI-digested and purified pENTR backbone (Thermo Scientific ...