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Citations for New England Biolabs :
3951 - 4000 of 10000+ citations for Human Dachshund Homolog 1 DACH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: Point mutations in all constructs were made by using Q5 Site-Directed Mutagenesis Kit (NEB). Primers were designed with NEBase Changer ...
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bioRxiv - Developmental Biology 2020Quote: ... and T7 RNA polymerase from HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB). Cold-anesthetized female mosquitoes were microinjected with 2.0 µg gene-specific dsRNA using a Nanoject II microinjector (Drummond Scientific Company ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA synthesis was carried out by ProtoScript-II first strand cDNA synthesis kit (NEB, USA) with random hexamer primers ...
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bioRxiv - Microbiology 2021Quote: ... pLentiCRISPR-V2 backbone (Sanjana, Shalem and Zhang 2014) using Quick Ligation™ kit (M2200, NEB) and transduced into THP-1 WT cells using Lentiviral particles (Fisch et al ...
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bioRxiv - Microbiology 2020Quote: ... The resulting fragment was cloned using the NEBuilder HiFi DNA assembly kit (NEW ENGLAND BioLabs) into the pMiniMAD2 plasmid previously digested using the restriction sites BamHI and EcoRI ...
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bioRxiv - Biochemistry 2020Quote: ... The RNA was then purified using a Monarch RNA Clean-up Kit (New England BioLabs).
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bioRxiv - Biochemistry 2020Quote: DNA was purified using the Monarch® Genomic DNA Purification kit (New England BioLabs, T3010S). DNA immunoprecipitation and sequencing was performed as previously described ...
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bioRxiv - Bioengineering 2021Quote: Site directed mutagenesis was performed using Q5® Site-Directed Mutagenesis Kit from NEB (E0554S) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... We used NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, NEB) and followed the manufacturer’s manual with several exceptions:
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bioRxiv - Bioengineering 2021Quote: ... We used NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, NEB) and followed the manufacturer’s manual with several exceptions:
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bioRxiv - Bioengineering 2020Quote: RNA were transcribed using the HiScribe T7 high yield RNA synthesis kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Library construction was performed using the NEBNext UltraII DNA Library Prep Kit from NEB (E7645S). Indexed samples were run using the Illumina Next-seq 300-cycle kit ...
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bioRxiv - Biochemistry 2020Quote: ... the two DNA fragments were joined using Gibson Assembly® Cloning Kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Genes of interest were amplified with the One Taq One-Step RT-PCR kit (NEB) using 1 μg of RNA ...
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bioRxiv - Cell Biology 2020Quote: ... Library concentration and quality control were determined using NEBNext Library Quant Kit (New England Biolabs) and Agilent High Sensitivity D1000 ScreenTape System (Agilent ...
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bioRxiv - Molecular Biology 2021Quote: ... and cloned into a pcDNA3.1(+) vector using the Quick Ligation Kit (New England Biolabs M2200).
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bioRxiv - Biophysics 2020Quote: ... in-vitro transcription using the commercial HiScribe™ T7 High Yield RNA Synthesis Kit (NEB) gave rise to two complementary ssRNAs without any non-complementary nucleotides at their ends ...
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bioRxiv - Cancer Biology 2021Quote: ... The libraries were quantified using the NEBNext Library Quant Kit for Illumina (New England Biolabs) on a StepOne Plus Real-Time PCR (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2020Quote: ... Libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB E7645S) and NEBNext Multiplex Oligos for Illumina (NEB Set 1 E7335 and Set 2 E7500S) ...
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bioRxiv - Cancer Biology 2020Quote: ... The DNA fragments were ligated to Bar Code Adapters using Quick Ligation kit (NEB M2200L) and purified using Agentcourt AMPure Beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2021Quote: ... Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Biochemistry 2021Quote: ... and 2 μL T7 RNA Polymerase mix (HiScribe T7 High Yield RNA Synthesis Kit, NEB) were incubated at 37°C for 3 h ...
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bioRxiv - Bioengineering 2021Quote: ... The synthesized mRNA was purified using Monarch RNA Cleanup Kit (New England Biolabs, Ipswich, MA), thereby removing the template DNA ...
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bioRxiv - Cell Biology 2021Quote: ... Site-directed mutagenesis was performed using the Q5® site directed mutagenesis kit protocol (NEB). GST-LL5β was cloned into pGEX plasmid vector ...
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bioRxiv - Cell Biology 2021Quote: ... Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... The DNAse-treated RNA samples were then placed through a Monarch RNA Cleanup Kit (NEB) to ensure no carryover of contaminants ...
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bioRxiv - Synthetic Biology 2021Quote: ... The resulting DNA templates were column purified using a Monarch PCR&DNA Cleanup Kit (NEB) and quantified by their absorption on a NanoPhotometer (Implen) ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA libraries were constructed using NEB next ultra-directional library kit (New England Biolabs Inc) and sequencing was performed using the Illumina HiSeq X platform ...
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bioRxiv - Microbiology 2020Quote: ... DNA ends were repaired using the NEBNext Ultra II End Repair Kit (New England Biolabs) and a DNA linker (26 ...
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bioRxiv - Microbiology 2021Quote: ... Point mutations were introduced by site-directed mutagenesis using Phusion High-Fidelity PCR kit (NEB) using primers listed in Table S1.
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bioRxiv - Neuroscience 2020Quote: ... RNA was reverse-transcribed to cDNA using M-MuLV Reverse Transcriptase kit (NEB, Ipswich, MA) and cDNA was analyzed with QPCR.
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bioRxiv - Genetics 2021Quote: ... PCR amplicons were purified using the Monarch PCR & DNA cleanup kit (New England BioLabs T1030) or the QIAQuick PCR purification kit (Qiagen 28104 ...
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bioRxiv - Neuroscience 2020Quote: ... Insert and vector fragments were ligated using HiFi DNA Assembly Kit (New England Biolabs, USA) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... To convert T05C1.4b cDNA to T05C1.4a we used the Q5 Site-Directed Mutagenesis Kit (NEB) and primers gtcatactcaacatctaATTGCGGAAAATGCATGC and catcatcaatatttacaTTATTACGATTTTGTCGCATAAAATTC
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bioRxiv - Microbiology 2021Quote: ... PhyR1 and PhyR2 proteins were overexpressed and purified using the IMPACT kit (New England Biolabs) following the manufacturer’s instructions and equal procedures for the four of them ...
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bioRxiv - Molecular Biology 2021Quote: All constructs were cloned using the NEBuilder HiFi DNA Assembly kit (New England Biolabs E2621) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted using the Monarch® Total RNA Miniprep Kit (New England Biolabs) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... coli MG1655 genomic DNA into pKK223-3 by NEBuilder HiFi DNA Assembly Cloning Kit (NEB) and the first 300 bp portion of leuA was translationally fused with the nano-Luc (nLuc ...
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bioRxiv - Microbiology 2021Quote: The pCDNA3.1-hACE2-FLAG plasmid and a Q5 Site-Directed Mutagenesis Kit (New England Biolabs) were used according to the manufacturer’s specifications to generate recombinant plasmids that expressed hACE2 N-glycosylation mutants ...
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Factors required for adhesion of Salmonella enterica serovar Typhimurium to lettuce (Lactuca sativa)bioRxiv - Microbiology 2020Quote: ... Amplified products were reassembled by site-directed mutagenesis (SDM) kit according to manufacturer’s protocol (NEB). Sequence-confirmed plasmids were electroporated in STM SR11 Δ12.
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bioRxiv - Molecular Biology 2021Quote: ... followed by plasmid DNA isolation using the Monarch® Plasmid Minprep kit (New England Biolabs) or a NucleoBond® Xtra Midi kit (Machery-Nagel) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCRs were performed using the Q5® High-Fidelity DNA Polymerase kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Specific primers were designed following the instructions of the Q5 site-directed mutagenesis kit (NEB) (primers 35-38 ...
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bioRxiv - Microbiology 2019Quote: ... The reacted DNA was then purified using PCR clean-up kit (New England Biolabs®) and quantified using the nanodrop ...
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bioRxiv - Microbiology 2019Quote: ... sgRNA sequences were cloned using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs). Primers were designed using the NEBaseChanger™ tool (v.1.2.9) ...
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bioRxiv - Microbiology 2020Quote: ... we introduced the desired mutations using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) into pfixK.21 pfixK contains the AU0158 fixLJ sequence along with 670 bp upstream within the pUC18-mini-Tn7-Tp back bone allowing for stable chromosomally integration at an attTn7 site.23,50 Point mutations were verified by Sanger sequencing ...
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bioRxiv - Cancer Biology 2020Quote: ... Libraries were prepared using the NEBNext Ultra Directional RNA Library prep kit (NEB cat # E7420S). Libraries were then sequenced on the Illumina NextSEQ500 ...
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bioRxiv - Cancer Biology 2020Quote: ... Libraries were prepared using the NEBNext Ultra II DNA Library prep kit (NEB cat # E7645). 3 cycles of PCR amplification were used for native inputs ...
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bioRxiv - Cancer Biology 2020Quote: ... with 500ng input RNA or Ultra RNA Library Prep Kit for Illumina (New England Biolabs). An Illumina HiSeq 2500 was utilized to performed paired-end sequencing.
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bioRxiv - Cancer Biology 2020Quote: ... were generated using NEBNext Single Cell/Low Input RNA Library Preparation kit (New England Biolabs) and sequenced in a NextSeq500 sequencer SE75bp (Illumina) ...