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Citations for New England Biolabs :
351 - 400 of 4860 citations for NK 1 Receptor Rabbit Polyclonal affinity purified biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 4SU-labeled RNA (100 µg) was biotinylated with HPDP-biotin (cat. 21341, ThermoScientific) and captured on 150 µL of streptavidin magnetic beads (NEB) per RNA sample ...
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bioRxiv - Molecular Biology 2021Quote: ... purified DNA was digested with I-SceI (NEB) and AvrII (NEB ...
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bioRxiv - Bioengineering 2020Quote: ... The purified amplicons were cloned into BsmBI (NEB)-digested CROPseq-Guide-Puro plasmids (Addgene #86708 ...
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bioRxiv - Microbiology 2019Quote: ... Purified RNA was treated DNAse (New England Biolabs) followed by EDTA ...
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bioRxiv - Genetics 2020Quote: ... and purified using a PCR purification kit (NEB).
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bioRxiv - Microbiology 2021Quote: ... purified using Monarch Gel Extraction Kit (NEB, USA) and ligated with SmaI-digested and dephosphorylated pUC19 vector using Blunt/TA Ligase Mastermix (NEB ...
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bioRxiv - Bioengineering 2021Quote: ... and purified using Monarch RNA cleanup kits (NEB).
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified with amylose resin (NEB) and eluted with 20 and 50 mM maltose (Sigma).
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bioRxiv - Plant Biology 2021Quote: ... and purified on amylose columns (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... All proteins were purified using Amylose Resin (NEB) or HisPur Cobalt Resin (Thermo ...
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bioRxiv - Cell Biology 2021Quote: ... purified with RNA clean up kit (NEB T2050), and cDNA was generated with SuperScript III Reverse Transcriptase (Thermo Scientific 18080044 ...
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bioRxiv - Biophysics 2021Quote: ... and 5 mg/mL purified BSA (NEB, G9001S), to prevent non-specific interaction of Cy5-eIF4E with the chip surface ...
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bioRxiv - Genomics 2022Quote: ... RNA was purified using streptavidin beads (NEB, S1421S) and removed from beads using Trizol (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... The purified DNA was phosphorylated with PNK (NEB), self-ligated with T4 ligase (NEB) ...
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bioRxiv - Molecular Biology 2019Quote: ... The protein was purified using amylose resin (NEB) using the provided protocol ...
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bioRxiv - Biochemistry 2020Quote: ... and purified according to the manufacturer’s protocol (NEB), followed by additional purification through a HiLoad 16/600 Superdex 75 gel filtration column (GE Healthcare ...
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bioRxiv - Synthetic Biology 2021Quote: ... Duplicate reactions were purified (T1030L, New England Biolabs) and eluted in nuclease-free water (12 μL) ...
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bioRxiv - Genetics 2021Quote: ... purified using Monarch DNA Gel Extraction Kit (NEB), and quantified using Qubit fluorometric quantification (Thermo Fisher Scientific).
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bioRxiv - Genetics 2019Quote: ... column-purified and transformed in 14 electroporations (NEB 10-beta Electrocompetent E ...
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bioRxiv - Biophysics 2022Quote: ... and purified using amylose resin (New England Biolabs). The protein was eluted with buffer A (10 mM Tris pH 7.5 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The assembled DNA constructs were purified from NEB XL10 Gold or DH5α competent cells (NEBC2987H ...
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bioRxiv - Genomics 2022Quote: ... and purified using RNA Cleanup kit (NEB, T2040S) then poly-A selected using NEBNext polyA magnetic isolation (NEB ...
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bioRxiv - Genomics 2022Quote: ... and purified using RNA Cleanup kit (NEB, T2040S) following up with the above protocol.
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bioRxiv - Molecular Biology 2022Quote: ... RNA was purified by Streptavidin Magnetic Beads (NEB) in Binding buffer (10 mM Tris-HCl pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... and purified with Monarch RNA Cleanup kit (NEB). RNA concentration was measured using Nanodrop ...
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bioRxiv - Biochemistry 2023Quote: ... Purified DCAF16 was dephosphorylated with lambda-phosphatase (NEB) at 4 ºC overnight ...
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bioRxiv - Molecular Biology 2023Quote: Purified RNA was treated with polynucleotide kinase (NEB) to prepare 5’ and 3’ ends for library construction as described for LORAX-seq.
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were purified by Amylose resin (NEB) and eluted with 10 mM maltose ...
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bioRxiv - Biochemistry 2023Quote: ... Purified plasmid DNA was linearized with AscI (NEB). Barcodes were amplified with 25 cycles of PCR using the entire sample of extracted DNA from each cell population to ensure complete sequencing coverage ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA products were column-purified (New England Biolabs) and verified by glyoxal agarose gels (ThermoFisher).
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bioRxiv - Molecular Biology 2021Quote: ... The 5’-end of dephosphorylated mRNAs were then labeled using 10 units of T4 PNK (NEB) and 0.5 µl [γ-32P] ATP (6000 Ci/mmol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Sense and anti-sense digoxigenin labeled probes were then produced using Taq polymerase (New England Biolabs) and 40 cycles of PCR with either the sense or anti-sense primer in a Taq polymerase PCR mixture to which 0.067 mM of Digoxigenin-5-aminoallyl-dUTP (Jena Bioscience GmbH ...
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bioRxiv - Genomics 2021Quote: ... DNA samples were labeled with 10 μL of a cocktail of repair enzymes (PreCR mix, NEB), fragmented ...
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bioRxiv - Systems Biology 2022Quote: ... Snap-EGFR was labeled with 0.5 μM Snap-Surface Alexa647 (New England Biolabs GmbH, Frankfurt, Germany) for at least 60’ ...
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bioRxiv - Cell Biology 2019Quote: ... SNAP-FOR1(3P,FH2) protein was labeled with SNAP-549 dye (New England Biolabs, Ipswich, MA) as per manufacturer’s instructions prior to each TIRF experiment.
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bioRxiv - Molecular Biology 2020Quote: ... 5uL of genomic DNA was labeled using a 50uL reaction with 5U Klenow exo-(NEB M0212L) and α-dCTP (Perkin Elmer BLU513H500UC ...
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bioRxiv - Cell Biology 2023Quote: ... Stable transformants were labeled with 100 nM SNAP-Cell 647-SiR ligand (New England Biolabs, S9102S). The fluorescence signals from SNAP-Cell 647-SiR were detected ...
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bioRxiv - Molecular Biology 2023Quote: ... Ribosomes with a spytag labeled L17 subunit was used during in vitro translation system (NEB, E3313S) to generate the stalled RNC ...
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bioRxiv - Genetics 2023Quote: ... All probes were 5’ end-labeled with [alpha-32P]-ATP and T4 polynucleotide kinase (NEB Inc.). The gels were exposed to a PhosphorImager ...
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bioRxiv - Cell Biology 2023Quote: ... cells were labeled with 5 μM cell-impermeable SNAP-Surface Alexa Fluor 488 (New England Biolabs) in complete media (DMEM + 10% FBS ...
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bioRxiv - Genetics 2024Quote: ... All probes were 5’ end-labeled with [alpha-32P]-ATP and T4 polynucleotide kinase (NEB Inc.). The gels were exposed to a Phosphor Imager ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with primary antibody rabbit anti-c-Fos (1:3000, New England Biolabs cat. No. 2250S) for 24 hours at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Enzyme restriction with DpnII was performed in 30 µl reaction mix (1 µl DpnII, 3 µl DpnII buffer, 10 µl purified DNA and 16 µl H2O; New England Biolabs, Ipswich, MA) and incubation at 37°C for 2h ...
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bioRxiv - Neuroscience 2022Quote: ... the 181-bp and 376-bp amplicon products (Figure 1-figure supplement 1A) were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA) and sent for Sanger DNA sequencing at the OHSU Vollum Sequence Core (Portland ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was purified from 1 μg of total RNA using an NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, USA) and following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... at 37° C for 2 hrs from 1 µg purified dsDNAs using a T7 high yield RNA synthesis kit (NEB, Cat # E2040S) in a total volume of 20 µL ...
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bioRxiv - Biophysics 2021Quote: ... The Biotin-handle and Cosmid-I95 DNA were both digested for 2 hours at 37°C with SpeI-HF (NEB, R3133L) and subsequently heat inactivated for 20 minutes at 80°C ...
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bioRxiv - Developmental Biology 2022Quote: ... dTTP (250 uM final concentration of each) and 250 uM final concentration of biotin-14-dATP and 50U DNA polymerase I Large (Klenow) Fragment (NEB, M0210) within 1x NEBuffer 3.1 ...
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bioRxiv - Genomics 2023Quote: Non-informative biotin at restriction fragment ends was removed by incubating the samples with dATP and T4 DNA polymerase (New England Biolabs #M0203S). After purifying the DNA again with a phenol:chloroform:isoamyl alcohol extraction ...
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bioRxiv - Cancer Biology 2020Quote: ... The synthetic genes encoding WT human TRIM28 with an N-terminal maltose binding protein (MBP) affinity tag (UniProt: Q13263) were subcloned to the pMAL-p2X vector (New England Biolabs, Beverly, MA, USA).