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Citations for New England Biolabs :
351 - 400 of 8425 citations for NADH dehydrogenase ubiquinone 1 alpha subcomplex assembly factor 3 NDUFAF3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Gibson assembly (New England Biolabs), and QuikChange Lightning site-directed mutagenesis (Agilent Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... through Gibson assembly (NEB E2621). A vector for expression of LDHAI141T was generated through a similar method using overlap extension PCR to introduce the LDHAI141T mutation ...
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bioRxiv - Biochemistry 2022Quote: ... or by Gibson assembly (NEB) following the protocol supplied by the manufacturer.
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bioRxiv - Cell Biology 2022Quote: ... using Gibson Assembly kit (NEB). To test the interaction of KRP4 and KRP5 ...
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bioRxiv - Biochemistry 2023Quote: ... using Gibson assembly cloning (NEB). Baculovirus was generated using the Bac-to- Bac system in suspension culture Sf9 cells according to the manufacturers protocols (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... All cloning was performed by NEB HiFi assembly (New England Biolabs) and validated by whole plasmid sequencing (Plasmidsaurus).
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bioRxiv - Cell Biology 2022Quote: ... NEBuilder HiFi assembly (NEB #E2621S), and transformation into chemically competent E ...
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bioRxiv - Biophysics 2024Quote: ... using Gibson Assembly (NEB E5520S) to contain an additional N-terminal cysteine for fluorophore labeling ...
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bioRxiv - Microbiology 2024Quote: ... through Gibson Assembly (NEB, E2611) using the following primer ...
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bioRxiv - Microbiology 2024Quote: ... Gibson assembly cloning kit (NEB), Ficoll-paque plus solution (GE Healthcare) ...
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bioRxiv - Genomics 2024Quote: ... or Golden Gate Assembly (NEB).
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bioRxiv - Neuroscience 2024Quote: ... using Golden Gate Assembly (NEB). Cells were then co-transfected with the plasmid with gRNAs ...
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bioRxiv - Neuroscience 2024Quote: ... using HiFi DNA Assembly (NEB). pGL4.10 Firefly plasmid is a promoter-less vector for measuring the activity of promoter or enhancer sequences and contains Luc2 ...
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bioRxiv - Plant Biology 2024Quote: ... and Golden Gate Assembly (NEB, NEBridge Golden Gate Assembly Kit ...
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bioRxiv - Plant Biology 2024Quote: ... NEBuilder HiFi DNA Assembly (NEB, NEBuilder HiFi DNA Assembly Master Mix ...
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bioRxiv - Neuroscience 2024Quote: ... Subsequently HiFi DNA Assembly (NEB) was conducted using two distinct fragments ...
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bioRxiv - Neuroscience 2024Quote: Utilizing HiFi DNA Assembly (NEB), four distinct fragments were combined ...
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bioRxiv - Genomics 2024Quote: ... by Gibson Assembly (NEB, E2611L). Primers and sequences used for cloning are listed in Supplementary Table 1 ...
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bioRxiv - Cell Biology 2024Quote: ... using HiFi Assembly (E5520 - NEB).
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bioRxiv - Biochemistry 2024Quote: ... either a Gibson assembly (NEB) was performed according to manufacturer’s protocol followed by transformation ...
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bioRxiv - Genetics 2024Quote: ... using Gibson assembly (NEB, E2621L). The resulting plasmid contains two sgRNAs with the pattern of hU6-sgRNA1-mU6-sgRNA2 ...
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bioRxiv - Developmental Biology 2021Quote: Plasmid pJM1 (Pceh-23_L::mKate2::rgl-1) was generated using Gibson Assembly (NEB) of PCR products ...
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bioRxiv - Microbiology 2022Quote: ... pcDNA3.1.mNG2(1-10) was generated through gibson assembly (NEBuilder, New England Biolabs) of a pcDNA3.1 vector (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... sgCD81i-1 g[9nt] and g[8nt] guides required golden gate assembly (NEB) due to the short length of the oligonucleotides ...
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bioRxiv - Bioengineering 2022Quote: ... Then CaCl2 was added to 2mM and 1 µL (about 1 µg) of Factor Xa (NEB) was added per 50 µg of protein and the samples were left at RT for 20-24h ...
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bioRxiv - Molecular Biology 2020Quote: ... NEB 5-alpha F’Iq cells (New England Biolabs) were used as the recipient strain for all plasmid constructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Escherichia coli NEB- 5-alpha (New England Biolabs) was used for cloning and BL21(DE3 ...
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bioRxiv - Synthetic Biology 2023Quote: Plasmids were transformed into BL21-alpha cells (NEB) and plated overnight at 37 □C on kanamycin LB plates (50 μg/mL) ...
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bioRxiv - Microbiology 2023Quote: ... coli strain NEB 5-alpha (New England Biolabs) (Table S5).
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bioRxiv - Developmental Biology 2024Quote: ... and NEB 5-alpha Competent E.coli (NEB C2987H) were used ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli NEB 5-alpha cells (New England Biolabs). Part plasmids and the pET21b(+ ...
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bioRxiv - Genetics 2024Quote: ... coli (NEB® 5-alpha Competent E. coli), and successful genetic manipulation was confirmed with sequencing ...
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bioRxiv - Biophysics 2022Quote: ... 2 µL factor mix (NEB), 250 nM pre-incubated 50S subunit ...
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bioRxiv - Microbiology 2020Quote: ... Gibson assembly protocol using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich, MA) was followed to ligate the VP1456 or the VPA0356 fragment with the expression vector pBAD33 ...
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bioRxiv - Molecular Biology 2020Quote: ... The vector fragments and homology arms were cloned by Gibson Assembly (NEB HiFi Assembly Kit).
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bioRxiv - Molecular Biology 2021Quote: ... Gibson assembly was performed in 1x Gibson Assembly® Master Mix (New England Biolabs E2611) for 1 hour at 50°C with 1:5 ratio of vector to inserts.
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bioRxiv - Cell Biology 2021Quote: ... Fragment assembly was carried out using NEBuilder HiFi DNA assembly Master Mix (New England Biolabs) according to manufactureŕs protocol ...
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bioRxiv - Biochemistry 2021Quote: ... All constructs were generated by standard cloning or by Gibson Assembly (NEBuilder HiFi Assembly, NEB) using XL10-Gold bacteria (Agilent) ...
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bioRxiv - Plant Biology 2020Quote: ... and Gibson DNA assembly with the NEBuilder HiFi DNA assembly master mix (New England Biolabs). Primers used for vector construction are listed in Tables S2 and S3 ...
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bioRxiv - Genomics 2021Quote: ... with the following modifications: assembly was performed using NEB Gibson Assembly mix (New England Biolabs) using a ratio of 200 ng vector ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plasmids were constructed by Gibson Assembly using NEBuilder® HiFi DNA Assembly Master Mix (NEB) or ligation using T4 DNA ligase (NEB) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Gibson assembly was performed with the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... B1-B2 fragments for Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (NEB E2621S) to generate pLKO.1-WT-Paxillin-T2A-GFP ...
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bioRxiv - Microbiology 2020Quote: Virus-encoding plasmids were generated using Gibson Assembly (NEB Gibson Assembly kit, NEB, Ipswich, MA). Insert amplicons containing 25 bp overlaps to target regions were generated using Kapa HiFi Hotstart™ high-fidelity polymerase (Kapa Biosystems/Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: Virus-encoding plasmids were generated using Gibson Assembly (NEB Gibson Assembly kit, NEB, Ipswich, MA). Insert amplicons containing 25 bp overlaps to target regions were generated using Kapa HiFi Hotstart™ high-fidelity polymerase (Kapa Biosystems/Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... These fragments were used for Gibson assembly using Gibson Assembly Master Mix (New England Biolabs). PCR products were produced with the Q5 High-Fidelity 2X Master Mix (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2022Quote: ... and assembled with Gibson assembly protocol using NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621S). Assembled DNA was transformed into competent cells prepared by the CCMB80 buffer (TekNova ...
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bioRxiv - Molecular Biology 2022Quote: ... Gibson assembly was performed with these products using the Gibson assembly master mix (NEB E2611) with an incubation time of 20 minutes room temperature ...
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bioRxiv - Genetics 2020Quote: ... with HiFi assembly and an NEBuilder HiFi DNA Assembly Cloning Kit (E5520S, New England BioLabs) to create pENTR-BLRR ...
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bioRxiv - Synthetic Biology 2021Quote: ... and was made suitable for GGA cloning using Gibson assembly (NEB Gibson Assembly Master Mix) of PCR products of the vector (NEB Phusion High-Fidelity DNA Polymerase ...