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Citations for New England Biolabs :
351 - 400 of 1632 citations for 10 PROPOXY DECANOIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... with 10 U of T4 Polynucleotide kinase (NEB) for 1 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... 10 µl of microRNA Marker (New England Biolabs) were mixed with 2.5 µl Low Range ssRNA Ladder (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... coli (NEB 10-beta, New England Biolabs C3020K), and plated at limiting dilutions on LB+ampicillin plates ...
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bioRxiv - Microbiology 2022Quote: ... coli (NEB 10-beta, New England Biolabs C3020K), and plated at limiting dilutions on LB+ampicillin plates ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 ng/μl ET SSB (New England Biolabs), 3 mM MnCl2 (Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... and 10 μL 2X RNA Loading Dye (NEB), denatured at 95° for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μM (nucleotide) linear φX174 dsDNA (N3021L, NEB) was added to the mixture to initiate the three-strand exchange reaction ...
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bioRxiv - Genomics 2022Quote: ... and 10 uL 2% BSA (New England Biolabs) for sample resuspension and dilution prior to 10x Genomics chip loading ...
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bioRxiv - Genomics 2022Quote: ... 10 μL of 2% BSA (New England Biolabs), and 984 μL of nuclease-free water 1xST buffer was prepared by diluting 2x ST with ultrapure water (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... coli strain NEB 10-beta (NEB Cat# C3019) was used for construction of plasmids by ligation-less Gibson assembly (Fu et al ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µl of RNase-free DNase I (NEB) was added to stop the reaction ...
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bioRxiv - Synthetic Biology 2023Quote: ... into 100 µL 10-beta electrocompetent cells (NEB), and then plated on 10 15 cm LBSpect agar plates for 16-20 h (37 °C ...
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bioRxiv - Genetics 2023Quote: ... 10 μL of 20mg/mL Proteinase K (NEB) was added to each well and samples were incubated at 65°C for 30 minutes ...
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bioRxiv - Genomics 2023Quote: ... 0.5 μl of 10 mM dNTPs (NEB, N0447L), 0.25 μl of Q5 Hot Start DNA Polymerase (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μL of 10 mM dNTPs (NEB N0447L), 1 μL GC2 polymerase ...
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bioRxiv - Molecular Biology 2023Quote: ... coli NEB 10-beta cells (New England Biolabs) and all constructs were sequence verified using plasmidsaurus Oxford Nanopore long read sequencing ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... 10 µl 2× Gibson Assembly Master Mix (NEB)) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli (NEB 10-beta, New England Biolabs C3020K), and plated at limiting dilutions on LB+ampicillin plates ...
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bioRxiv - Genomics 2023Quote: ... 2 μL of 10 mM ATP (NEB P0756S), 1 μL of E ...
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bioRxiv - Immunology 2023Quote: ... were used along with 10% BSA (NEB #B9200) as a blocking agent ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli (NEB 10-beta, New England Biolabs C3020K), and plated at limiting dilutions on LB+ampicillin plates ...
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bioRxiv - Neuroscience 2023Quote: ... coli strain NEB 10-beta (New England Biolabs) was used for all cloning ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µl T4-PNK (10 U/µl, NEB) and 9.5 µl H2OMQ were added ...
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bioRxiv - Bioengineering 2024Quote: ... and 10 U of DNA polymerase I (NEB) was added to the samples on ice for a final volume of 50 μl ...
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bioRxiv - Molecular Biology 2024Quote: ... 1μl RNA ligase 10 000 U/μl (NEB) and 1 μl RppH 5000 U/μl (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... the corresponding single and multiple amino acid substitutions were introduced by QuikChange site-directed mutagenesis (NEB, Phusion High-Fidelity PCR Kit) followed by KLD (Kinase ...
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bioRxiv - Neuroscience 2020Quote: ... amino acid sequence identical to GenBank: BC002453.2) was amplified using Q5 High-Fidelity DNA Polymerase (New England Biolabs, Ipswich MA, USA) and inserted into the TOPO cloning site in pET151/D-TOPO E ...
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bioRxiv - Molecular Biology 2021Quote: ... or more amino acid substitutions in SecPH with Q5 PCR methodology (Q5®Site-Directed Mutagenesis Kit, New England Biolabs, E0554) or PCR site-directed mutagenesis strategy ...
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bioRxiv - Immunology 2021Quote: ... were generated by introducing the corresponding amino acid mutations (Extended Data Fig. 5) using the Q5® Site-Directed Mutagenesis Kit (NEB) and per manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... The OprCAA mutant was produced by changing the key amino acids Cys143 and Met147 to alanine residues using the KLD Quickchange site-directed mutagenesis kit (New England Biolabs, UK) and specific primers containing both mutation sites (forward ...
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bioRxiv - Cell Biology 2019Quote: ... The cDNA coding region corresponding to RDGB(USR1-LNS2)Δ (amino acids 1-472) was subcloned into pUAST-attB by using the restriction enzymes NotI and XbaI (NEB). Similarly ...
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bioRxiv - Cell Biology 2023Quote: ... The C-terminal truncation of murine STING (to include only amino acids 1-339) was accomplished using NEB Q5 High-Fidelity 2X Master Mix (NEB M0492S) per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... 948 μL of ligation master mix was then added: 150 μL of 10× NEB T4 DNA ligase buffer with 10 mM ATP (NEB, B0202), 125 μL of 10% Triton X-100 ...
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bioRxiv - Genomics 2019Quote: ... 948 µL of ligation master mix was then added: 150 µL of 10× NEB T4 DNA ligase buffer with 10 mM ATP (NEB, B0202), 125 µL of 10% Triton X-100 ...
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bioRxiv - Microbiology 2020Quote: ... After adding 10% 1M sodium dodecyl sulphate (SDS) and 10 μL of 8 U/mL proteinase K (NEB, #P8107S, Ipswich, MA), the suspension was then incubated at 56°C for 4 hours ...
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bioRxiv - Systems Biology 2020Quote: ... 10 mM DTT, 12% PEG 8000, 1 mM each dNTPs, 10 µM dN-SMRT oligo, 5 µl Klenow enzyme NEB #M0212) for 1 h at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... Purified PCR products or annealed oligonucleotides were prepared as described above and end-labeled with 10 μCi [γ-32P]ATP (Perkin-Elmer) and 10 U T4 polynucleotide kinase according to established protocols (New England BioLabs). Binding reactions included 1 μl (~30 ng ...
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bioRxiv - Genomics 2019Quote: ... Sonicated DNA was next incubated for 30 min at 20°C with 20 μl 10× end-repair buffer and 10 μl end-repair mix (NEB E6050S). The reaction was next cleared by 2× AmpureXP beads (Agencourt AMPure XP ...
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bioRxiv - Microbiology 2019Quote: ... 1 μmol of oligo DNA primer was incubated with 10 μCuries of [γ-32P]ATP and 10 U of T4 PNK (New England Biolabs) in 70 mM Tris-HCl (pH 7.6 ...
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bioRxiv - Cell Biology 2019Quote: ... were performed with 5-10 μM final protein concentration and 10 μM dye (SNAP-Surface Alexa Fluor488 (New England Biolabs S9129S) or SNAP-Surface Alexa Fluor647 (New England Biolabs S9136S) ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 µl Forward Stagger Mix (10 µM) and 2 µl Reverse Index Primer (10 µM) specific to each vector backbone and Nuclease-free water (NEB,USA) up to 50 µl ...
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bioRxiv - Plant Biology 2023Quote: ... Type-IIS cloning was performed as described previously (Cai et al., 2020) using a Master Mix containing 10% (v/v) 10× T4 DNA ligase buffer (NEB #M0202), 2.5% (v/v ...
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bioRxiv - Biophysics 2023Quote: Plasmid pRS303 CBP-TEV-halo-orc3 gal1-10 orc4 was generated by cloning the CBP-TEV-halo sequence from plasmid pRS306-CBP-TEV-halo-Pri1-Gal1-10 Pri2 into plasmid pRS303-orc3-Gal1-10 orc4 through Gibson assembly (NEB #E2611L) using primers TL-446 ...
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bioRxiv - Microbiology 2024Quote: ... 100 pmol oligonucleotide was incubated with 10 µCi [γ-32P]-ATP in the presence of 10 U T4 polynucleotide kinase (NEB) at 37°C for 2 hours ...
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bioRxiv - Biophysics 2019Quote: ... HIV-1 MAG2A-ΔHBR-EGFP was generated from HIV-1 MAG2A-EGFP by deleting amino acids 18-32 using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA). pEGFP-N1 and pEYFP-N1 were obtained from Clonetech (Mountainview ...
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bioRxiv - Microbiology 2021Quote: ... nucleic acid (5 μL) was used as template for reverse transcription using LunaScript® RT SuperMix Kit (New England Biolabs, Hitchin, UK) in 20 μL reaction volume ...
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bioRxiv - Microbiology 2021Quote: ... The first EcoRI restriction site at 829 bp within the AgeI and KasI restriction sites in RGD4C-AAVP-TNF was deleted in two steps to mutate a thymidine to cytosine nucleotide at position 833 without altering the translated amino acid using the Q5 site-directed mutagenesis kit (New England Biolabs, Ipswich, MA) by following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: The viral nucleic acids detected by PCR of the LTR-gag region were digested with the restriction endonuclease ScaI (New England BioLabs, Beverly, MA), which cuts within the amplicon of HIV-2287 but not SIVmne ...
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bioRxiv - Biochemistry 2023Quote: Amino acid sequencing and glycan localization were carried out by digesting the antibody samples with trypsin or chymotrypsin (New England Biolabs, Ipswich, MA) followed by LC-MS/MS analysis of proteolytic fragments with an Orbitrap Fusion (Thermo ...
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bioRxiv - Cell Biology 2019Quote: ... we combined 10 μg of λ-DNA (NEB, N3011S) and 5 μl of a 10X polymerase buffer (50 mM Tris-HCl ...