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Citations for New England Biolabs :
3901 - 3950 of 10000+ citations for Human Carboxyl Terminal PDZ Ligand Of Neuronal Nitric Oxide Synthase Protein NOS1AP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... PCR amplifications were performed using Phusion High-Fidelity PCR kit (New England Biolabs #M0530L) according to manufacturer’s protocol using 50 ng template DNA per 100µL reaction and 18 cycles ...
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bioRxiv - Synthetic Biology 2024Quote: ... Recovery products were then purified with the Monarch® PCR & DNA Cleanup Kit (NEB) and eluted in 10 µl of the Monarch® DNA Elution Buffer ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was used as a template for PCR (Phusion HF kit (New England Biolabs)) with primers designed to amplify the spike and HE genes (Table 2) ...
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bioRxiv - Immunology 2024Quote: ... transcribed in vitro using HiScribe™ T7 High Yield RNA Synthesis Kit (#E2040S, NEB) and purified with RNA Clean & Concentrator™ (#R1017 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... were transcribed and purified using HiScribe T7 High Yield Transcription Kit (New England BioLabs) and illustra Microspin G-50 Columns (GE Healthcare Life Sciences) ...
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bioRxiv - Developmental Biology 2024Quote: ... and RNA was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs). cDNA was made using iSCRIPT reverse transcriptase (BioRad) ...
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bioRxiv - Cell Biology 2024Quote: Prior to rRNA depletion using the NEBNext rRNA Depletion Kit v2 (New England Biolabs), 1 μg total RNA was mixed with 2 μl 1:100 diluted ERCC RNA Spike-In Mix (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were prepared using NEBNext UltraTMII DNA Library Prep Kit for Illumina (NEB, E7645) following manufacturers instructions ...
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bioRxiv - Biochemistry 2022Quote: ... purified KRAS protein was mixed with GMP-PNP (molar ratio of 10:1 GMP-PNP:KRAS) and calf intestinal alkaline phosphatase (NEB cat# M0290, 3 units per mg of KRAS). The reaction mixture was incubated for 3 hours at room temperature and then purified by size-exclusion chromatography on a 10/300 Superdex 75 GL column (GE Healthcare ...
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bioRxiv - Plant Biology 2020Quote: ... Sequencing libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA), and the quality was assessed using the Agilent 2100 Bioanalyzer ...
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bioRxiv - Plant Biology 2020Quote: ... Stranded libraries were prepared using the NEBNext Ultra Directional RNA Library Kit (New England Biolabs), barcoded ...
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bioRxiv - Biophysics 2021Quote: ... sgRNA was in vitro transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). Samples were incubated at 37°C for 16 hours before purifying the sgRNA with Invitrogen TRIzol Reagent (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... The cDNA was purified using the Monarch DNA and PCR Cleanup Kit (New England Biolabs). A region around an A-to-I editing site in BPNT1 was amplified by Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs ...
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Chromatin accessibility changes at intergenic regions associates with ovarian cancer drug resistancebioRxiv - Cancer Biology 2021Quote: Libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized with the First Strand cDNA Synthesis Kit (New England BioLabs, Ipswich, MA) and analyzed for expression of collagen I (COL1A1 ...
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bioRxiv - Physiology 2021Quote: ... Libraries were quantified with quantitative PCR (NEBnext Library quantification kit, New England Biolabs, Ipswich, USA) and a Agilent Bioanalyzer High Sensitivity DNA chip ...
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bioRxiv - Cell Biology 2019Quote: ... A sequencing library was constructed with NEBNext Ultr II RNA Library Prep Kit (NEB, E7770S) and amplified with NEBNext Multiplex Oligos (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... DNA concentration were measured using the NEBNext® Library Quant Kit for Illumina (E7630L, NEB). Each 50 μl PCR reaction was composed of 0.5 μM primers ...
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bioRxiv - Genetics 2021Quote: ... sgRNAs or crRNAs were transcribed using the HiScribe T7 High Yield RNA Synthesis kit (NEB) or the MEGAscript T7 Transcription kit (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2021Quote: The DNA template oligo was amplified using the Phusion DNA polymerase kit (NEB, cat# M0530S). Refer Table S1 for primer sequences ...
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bioRxiv - Microbiology 2021Quote: ... libraries were prepared using a NEB ultra II DNA Library Prep Kit for Illumina (NEB), quantified using a Kapa qPCR Library Kit (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... The capped mRNAs were in vitro synthesized with the HiScribe T7 ARCA mRNA Kit (NEB) and purified with an RNA Clean & Concentrator-25 kit (Zymo Research ...
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bioRxiv - Developmental Biology 2021Quote: mRNA was synthesized using the HiScribe™ T7 ARCA mRNA Kit (with tailing) (NEB, E2060S) and a PCR product amplified from the Kaede-NLS plasmid (addgene ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and constructed sequencing libraries with the NEBNext Ultra II RNA Library Prep kit (NEB #E7770) using dual indices (NEB #E7600) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Final concentration was measured by qPCR using the NEBNext Library Quant Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The repair template was cloned using the Gibson assembly kit (New England Biolabs, MA, USA). Suitable target sites without off-targets were identified using the CRISPR Optimal Target Finder (134 ...
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bioRxiv - Developmental Biology 2020Quote: ... First-strand complementary cDNA was synthesized with LunaScript™ RT SuperMix Kits (New England Biolabs). Quantitative PCR was performed with a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... 300ng of DNA were used for the library preparation using the Ultra II kit (NEB) for adaptation ...
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) and NEBNext Multiplex Oligos for Illumina (New England Biolabs ...
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bioRxiv - Genomics 2020Quote: ... Alternate allele plasmids were generated via site directed mutagenesis (Q5 SDM kit, New England Biolabs) using the following primers ...
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bioRxiv - Genomics 2020Quote: ... DNA-libraries were multiplexed with NEBNext Multiplex Oligos for Illumina kit (New England Biolabs, UK). According to standard Illumina cluster generation and sequencing protocols ...
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bioRxiv - Evolutionary Biology 2022Quote: ... This was performed as described by the protocol of Illumina sequence library preparation (kit: NEB - M6630 ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was extracted using the Monarch total RNA Miniprep Kit (New England Biolabs, #T2010) and used for Illumina stranded mRNA library prep ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and cDNA was synthesized using the ProtoScript II First Stand cDNA Synthesis Kit (NEB #E6560S). The experiment was replicated as follows ...
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bioRxiv - Molecular Biology 2019Quote: ... The resulting dsDNA was used to create libraries using the Ultra II library kit (NEB) as per the manufacturer’s instructions with 13 cycles at the amplification step.
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bioRxiv - Neuroscience 2021Quote: ... Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Molecular Biology 2021Quote: ... Single step PCR was performed using a One-Taq™ PCR reaction kit (NEB, USA) with 30 amplification cycles and 2 ng of original circular-DNA extract as the DNA template ...
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bioRxiv - Molecular Biology 2021Quote: ... In vitro transcribed RNAs were purified with an RNA Cleanup Kit (New England Biolabs, USA) and quantitated on a Nanodrop (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... HSV-SREBP-1c point mutants were prepared using the Q5 Site-Directed mutagenesis kit (NEB). Two RHBDL4 mutants were produced by PCR using primers containing mutation according to references (Fleig et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... The vector fragments and homology arms were cloned by Gibson Assembly (NEB HiFi Assembly Kit).
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bioRxiv - Neuroscience 2021Quote: The Safb1-ΔRE-HA mutant was generated using the Q5 Site-Directed Mutagenesis Kit (NEB). 5’-overlapping primers were designed and the Safb1-HA vector was amplified excluding the RE region (amino acids 552-708) ...
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bioRxiv - Molecular Biology 2021Quote: ... The abundant rRNAs were depleted from the sample using NEBNext rRNA Depletion Kit (NEB, #E6310). The rRNA-depleted RNAs were then subjected to RNA-seq library construction using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... psgaDC3XFLAG was obtained by ligating both amplicons using the Gibson Assembly® Cloning Kit (NEB), to replace acaDC with sgaDC ...
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bioRxiv - Molecular Biology 2020Quote: ... Mutations in the pRS315-Rpc160 plasmid were generated using the Gibson Assembly Cloning Kit (NEB) and clones were confirmed by sequencing the entire coding region ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep kit (NEB), indexed using NEBNext Multiplex Oligos (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were generated with the NEBNext Ultra Directional RNA Library Prep kit for Illumina (NEB) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified digestion products were ligated to Esp3I-treated LentiCRISPR-v2 using Gibson assembly kit (NEB) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA extracted by TRIzol-Chloroform or the RNA-Easy kit was treated with DNaseI (NEB) to remove DNA contamination ...
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bioRxiv - Cell Biology 2022Quote: ... we used the NEBNext Ultra II RNA library prep kit for Illumina (New England Biolabs) (9 cycles of amplification) ...
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bioRxiv - Cell Biology 2022Quote: 5’ Phosphorylated RA3 oligonucleotides were adenylated using 5’ DNA Adenylation Kit (New England BioLabs E2610S), by mixing 1 μL of 100 μM of 5’ Phosphorylated RA3 ...