Labshake search
Citations for New England Biolabs :
3801 - 3850 of 9360 citations for Rat CD325 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, Catalog #E7530L) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Molecular Biology 2023Quote: crRNA (Supplementary Table 2) was synthesized using a HiScribe T7 High Yield RNA Synthesis Kit (NEB). The DNA sequences includes the T7 promoter at the 5’ end and the sequence from crRNA with the target sequence at the 3’ end ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentration of libraries was determined using NEBNext library Quant kit for Illumina (NEB, Cat# E7630). Libraries were then pooled and sequenced by Macrogen on Ilumina Hiseq X-ten platform ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from ear punch biopsies using the Monarch Genomic DNA purification kit (NEB). Transgenes were identified using PCR primers specific for each transgene (Suppl ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by library preparation using the NEB Next Ultra II RNA Library Prep Kit (NEB E7770L). Sequencing was done using the Illumina Nextseq with 75-bp read length and single-end.
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bioRxiv - Molecular Biology 2023Quote: ... The pJC53.2_SmUbi-EGFP-SmUbi construct was assembled using the NEBuilder Hifi DNA Assembly Cloning Kit (NEB). Competent Escherichia coli DH5α (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... and the 3,240 bp fragment recovered from the gel (Monarch Gel Extraction Kit, NEB, Ipswich, MA). Components of the transgene construct ...
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bioRxiv - Molecular Biology 2023Quote: ... see below) by site-directed mutagenesis using the Q5 site-directed mutagenesis kit (New England Biolabs) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: RNA-seq libraries were prepared with NEBNext Ultra II Directional RNA-seq kit for Illumina (NEB) after rRNA depletion (NEBNext rRNA depletion kit by NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... Guide RNA were in vitro synthesized using the EnGen sgRNA Synthesis Kit (New England Biolabs, E3322V) cording to manufacturer’s recommendation ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were prepared using the NEBNext rRNA Depletion Kit v2 (Human/ mouse) (NEB #E7405), NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760 ...
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bioRxiv - Cancer Biology 2023Quote: ... and libraries for Illumina sequencing were prepared using the NEBNext Ultra II RNA kit (NEB, #E7770L). Quantification of the library was performed using dsDNA HS assay kit and Qubit (Molecular Probes ...
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bioRxiv - Developmental Biology 2023Quote: ... Sequencing libraries were generated using a NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, E7770S) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... mCh-Rab7A was cloned into a piggyBac plasmid using the NEB HIFI DNA Assembly Kit (NEB) and primers listed in Supp ...
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bioRxiv - Microbiology 2023Quote: ... and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) following the manufacturer’s directions ...
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bioRxiv - Genomics 2023Quote: ... and stranded libraries constructed using the NEBNext Ultra II directional RNA library preparation kit (NEB, E7760) following the protocol for ribosome-depleted RNA and with an 11-min RNA fragmentation step according to the manufacturer’s instructions ...
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Gene expression analyses reveal differences in children’s response to malaria according to their agebioRxiv - Microbiology 2023Quote: ... before preparation of stranded libraries using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB). cDNA libraries were sequenced on an Illumina NovaSeq 6000 to generate ∼55-130 million paired-end reads of 75 bp per sample ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then indexed and adaptor-ligated using NEBNEXT Ultra II DNA Library Preparation Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA-sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep kit for Illumina (NEB) combined with the NEBNext rRNA Depletion kit (NEB) ...
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bioRxiv - Genomics 2023Quote: ... and the sequencing libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The proGL2:EYFP:gl2ΔNLS construct was created using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs), taking wild-type GL2 cDNA in pENTR/D-TOPO as a template ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was then performed using Luna Universal One-step RT-qPCR kit (New England Biolabs) following the provided protocol on a BioRad CFX Opus 96 quantitative Real-Time PCR machine ...
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bioRxiv - Microbiology 2023Quote: ... The sequencing libraries were prepared with NEBNext rRNA Depletion Kit v2 (New England Biolabs, Ipswich, MA) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... We quantified the libraries using the NEBNext Library Quant Kit for Illumina (New England Biolabs, #E7630) based on the mean insert size provided by the Bioanalyzer ...
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bioRxiv - Neuroscience 2023Quote: ... The K262E point mutation was introduced with the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). pSecTag2A was used for control experiments ...
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bioRxiv - Biochemistry 2023Quote: ... the Myc/DDK tag was removed via the Q5 site-directed mutagenesis kit (New England Biolabs) using the oligonucleotides mS29 ΔMyc-DDK Forward ...
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bioRxiv - Microbiology 2023Quote: ... Prepared libraries were quantified using a NEBNext Library Quant kit for Illumina E7630L (New England BioLabs) and pooled with volumes adjusted to normalize concentrations aiming for ∼700-fold genomic coverage for population samples or ∼200-fold genomic coverage for clones ...
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bioRxiv - Genomics 2024Quote: ... a sequencing library was prepared using NEBNext® Enzymatic Methyl-seq Kit (NEB; catalog number E7120S). Library sequencing was performed using NextSeq 2000 (Illumina ...
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bioRxiv - Biophysics 2024Quote: ... and the resulting RNAs were isolated from the reaction mixtures by Monarch RNA Cleanup Kit (NEB). The size and purity of the RNA constructs was confirmed by TBE-urea PAGE (Supplementary Fig ...
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bioRxiv - Systems Biology 2023Quote: ... IVT was conducted using the HiScribe® T7 Quick High Yield RNA Synthesis Kit (NEB E2050S) - 1x T7 RNA Polymerase Mix ...
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bioRxiv - Microbiology 2024Quote: ... and cDNA library preparation (NEBNext Ultra II Directional RNA Library Prep Kit - Bacteria (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega. E. coli NEB 5α (NEB) was used as a host for plasmids ...
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bioRxiv - Physiology 2024Quote: ... Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega. E. coli NEB 5α (NEB) was used as a host for plasmids ...
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bioRxiv - Developmental Biology 2024Quote: ... plasmid templates were linearized with NotI and purified using Monarch PCR purification kits (New England Biolabs). The purified templates were transcribed using mMESSAGE mMACHINE Sp6 (Thermo-Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... and reversely transcribed into cDNA using the ProtoScript II NEB cDNA synthesis kit (New England Biolabs).
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bioRxiv - Genomics 2024Quote: ... Sequencing adapters were ligated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) while DNA was bound to the beads ...
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bioRxiv - Genomics 2024Quote: ... The recovered DNA was subjected to library construction using NEBNext Ultra DNA library prep kit (NEB). The sequencing libraries were amplified by PCR using the NEBNext Ultra II Q5 Master Mix (NEB ...
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bioRxiv - Microbiology 2024Quote: ... DNA was subsequently purified with a Monarch PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Val206 of Envy was mutagenized to Lys with the Q5 Site-Directed Mutagenesis Kit (NEB #E0554S) using primers listed in Table S2 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... After the reaction we purified the PCR product with Monarch PCR and DNA Cleanup Kit (NEB).
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of purified DNA template was used in the Hi-Scribe T7 transcription kit (NEB) at 37°C overnight (∼16 hr.) ...
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bioRxiv - Genomics 2024Quote: ... and final library amplification were performed using the NEBNext Enzymatic Methyl-seq Kit (New England BioLabs) for WGEM-seq libraries ...
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bioRxiv - Cancer Biology 2024Quote: ... was used for library preparation of total RNAs and Next small RNA Kit (New England Biolabs) for library preparation of microRNAs according to manufacturerś instructions ...
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Evolutionary Adaptation of an HP1-protein Chromodomain Integrates Chromatin and DNA Sequence SignalsbioRxiv - Genetics 2024Quote: ... Barcoded libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) according to manufacturer’s instructions and sequenced on a NovaSeqSP instrument (Illumina).
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bioRxiv - Cancer Biology 2024Quote: ... All single-cell libraries were prepared using the NEBNext Ultra II FS kit (New England Biolabs) following the manufacturer’s instructions but using one-fourth volumes ...
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bioRxiv - Genetics 2024Quote: ... Up to 1µg was the used for reverse transcription using the ProtoScript Reverse Transcription kit (NEB). qPCR was done using iTaq universal SYBR Green Supermix (BioRad ...
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bioRxiv - Genetics 2024Quote: ... A NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) was used for library preparation ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantitative RT-PCR was conducted with the Luna Universal One-Step RT-qPCR Kit (NEB, E3005) on a Bio-Rad CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... The R1R DNA adapter was adenylated by using a 5’ DNA Adenylation kit (New England Biolabs) and then ligated to the 3’ end of the cDNA by using Thermostable 5’ App DNA/RNA Ligase (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB, E7760S, USA) following the manufacturer’s recommendations ...