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Citations for New England Biolabs :
3751 - 3800 of 10000+ citations for Rat Heat Shock Protein Beta 2 HSPB2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitation was performed with the EpiMark N6-Methyladenosine Enrichment Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... the NEB Q5 Site-directed Mutagenesis Kit (New England BioLabs, USA) was used ...
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bioRxiv - Microbiology 2023Quote: ... The Luna Universal One-Step RT-qPCR Kit (New England Biolabs) was used for the detection of viral genomes in the heat-inactivated samples performed through reverse transcription quantitative polymerase chain reaction (RT-qPCR) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was purified using the Monarch RNA clean-up kit (NEB) and RNA length and quality was verified via 10% acrylamide gel.
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bioRxiv - Plant Biology 2023Quote: ... followed by NEB Ultra II RNA library prep kit for Illumina (New England Biolabs, catalog #E7770S ...
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bioRxiv - Immunology 2023Quote: sgRNAs were produced using the transcription T7 high yield kit (NEB) and purified with the EZNA micro-elute RNA clean up kit (Omega Bio-tek) ...
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bioRxiv - Plant Biology 2024Quote: ... followed by purification with the Monarch RNA Clean Up Kit (NEB). RNA (1 µg per sample ...
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bioRxiv - Systems Biology 2024Quote: ... followed by purification using a Monarch PCR&DNA Cleanup Kit (NEB). Ring-ligation was carried out using T4 DNA ligase (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid was extracted using Monarch Plasmid Miniprep Kit (New England BioLabs) from the propagated E ...
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bioRxiv - Genomics 2024Quote: ... the Monarch® HMW DNA Extraction Kit for Tissue (NEB, USA) was utilized in accordance with the manufacturer’s specifications ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA synthesis was performed using LunaScript® RT SuperMix Kit (NEB) following provider recommendation ...
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bioRxiv - Cell Biology 2024Quote: ... and purified using an NEB clean and concentrate kit (NEB #T2030).
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bioRxiv - Genomics 2024Quote: ... with the NEBNext Ultra II Directional RNA Library Prep kit (NEB). One of the three H ...
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bioRxiv - Microbiology 2024Quote: ... Fragments were purified using a gel agarose purification kit (NEB Monarch). Taq polymerase PCR with primers P7 and P8 ...
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bioRxiv - Microbiology 2024Quote: ... using Q5® Site-Directed Mutagenesis Kit (New England Biolabs; #E0554S). Guide RNA and repair template sequences are listed in Supplementary Data 3c.
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bioRxiv - Cancer Biology 2024Quote: ... using the NEB Ultra II Library Prep Kit (New England Biolabs) per manufacturer’s instructions and the libraries were sequenced at 50 bp read length and were sequenced on an NovaSeq SP with a target of ∼40M PE reads per sample.
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bioRxiv - Cancer Biology 2024Quote: ... using the NEB Ultra II Library Prep Kit (New England Biolabs) per manufacturer’s instructions and sequenced using Hiseq 4000 PE 150bp ...
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bioRxiv - Microbiology 2024Quote: ... using the NEBNext Library Quant Kit for Illumina (New England BioLabs) according to the instructions of the manufacturer ...
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bioRxiv - Genomics 2024Quote: ... Libraries were constructed using the NEBNext Enzymatic Methyl-seq Kit (NEB), following the manufacturer’s guidance ...
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bioRxiv - Cell Biology 2024Quote: ... and NEBnext Ultra II Directional RNA library prep kits (NEB #E7760L). Paired end 200 bp sequencing with additional reads for dual 8/8 indices plus the 11nt UMI after the i7 index was performed on an Illumina NovaSeq 6000 at the University of Chicago Genomics Facility.
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bioRxiv - Molecular Biology 2024Quote: ... LongAmp® Hot Start Taq DNA Polymerase kit (New England Biolabs) was used according to the manufacturer’s protocol to amplify the SEGS-1 region from DNA samples collected from 4 plants of each genotype ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid extraction and DNA purification were performed using Monarch kits (NEB). Sequences were verified with Sanger sequencing by Azenta Life Sciences or Primordium Labs for whole plasmid sequencing ...
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bioRxiv - Neuroscience 2024Quote: ... and then quantified by qPCR (NEBNext Library Quant Kit, NEB, E7630). 20 pM of DNA were loaded onto an Illumina MiSeq (v3 ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA was used with the EpiMark Bisulfite Conversion Kit (NEB) using the manufacturer instructions.
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bioRxiv - Immunology 2024Quote: ... and gRNAs were purified with the Monarch RNA Cleanup Kit (NEB), aliquoted ...
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bioRxiv - Genomics 2024Quote: Total RNA was extracted with Monarch Total RNA Miniprep Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and Biolux Cypridina luciferase assay kit (New England Biolabs, USA; #E3309), respectively ...
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bioRxiv - Synthetic Biology 2024Quote: ... Genomic DNA was extracted using Monarch Genomic DNA Purification Kit (NEB) according to manufacturer’s instructions and eluted in 20 µL water.
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bioRxiv - Cell Biology 2024Quote: ... or the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554), the DpnI (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified with the Monarch® PCR & DNA Cleanup Kit (NEB). An aliquot (1 µg ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using the Monarch Genomic DNA Purification Kit (NEB), and quality checked using a genomic Tapestation 4200 (Agilent Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... using the 2X Gibson Assembly Kit (Cat # E2611S, New England Biolabs). Assembled constructs were confirmed by whole plasmid sequencing (Plasmidsaurus ...
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bioRxiv - Microbiology 2024Quote: ... using the NEBNext Ultra II DNA Library prep Kit (E7370; NEB). Libraries were sequenced using an Illumina NextSeq 2000 sequencer 75 bp Single End.
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bioRxiv - Microbiology 2024Quote: ... Gibson Assembly® Cloning Kit was employed (New England BioLabs Inc) to make the deletion construct pEX-18Tc-femI ...
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bioRxiv - Microbiology 2024Quote: ... and cloned into pLGB13 using the NEBuilder HiFi cloning kit (NEB). The pLGB13 plasmid was linearized by double digest with BamHI and SalI restriction enzymes ...
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bioRxiv - Molecular Biology 2024Quote: ... dsRNA was purified using Monarch RNA Cleanup Kit (50 μg; NEB) and checked on 1% agarose gel and concentration measured with a NanoDrop One spectrophotometer (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... or the Monarch® DNA Gel Extraction Kit (New England Biolabs). In a few cases ...
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bioRxiv - Biochemistry 2020Quote: ... A pre-cleaning step was applied by incubating each sample with 10 μl of Protein A magnetic beads (New England Biolabs GmbH, Frankfurt, Germany) for 1 h at 4 °C in tube roller (15 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... The DNA encoding the EGFP-fusion proteins and the P2A peptide was inserted by Gibson assembly using NEBuilder HiFi DNA assembly master mix (New England BioLabs, Ipswich, MA, USA). The HeLa Flp-In T-REx K44A Dynamin2-EGFP-P2A-Caveolin1-mCherry construct was obtained by in vitro mutagenesis of the pcDNA/FRT/TO/Dyn2-EGFP-P2A-Caveolin1-mCherry construct exchanging lysine at position 44 to an alanin using these primers ...
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bioRxiv - Developmental Biology 2020Quote: ... A total of 863 Bicyclus embryos were injected with 300 ng/µl of guide and 300 ng/µl of Cas9 protein (NEB; Cat. no.: M0641) mixed together in equal parts (total volume = 10 µl ...
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bioRxiv - Developmental Biology 2019Quote: ... The bound protein (~42kDa, 360 amino acids) was released from the MBP tag by cleavage with Factor Xa (New England Biolabs, Massachusetts, United States) for 24 hours at 4°C in 20mMTris pH 7.2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The synthetic genes encoding WT human TRIM28 with an N-terminal maltose binding protein (MBP) affinity tag (UniProt: Q13263) were subcloned to the pMAL-p2X vector (New England Biolabs, Beverly, MA, USA).
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bioRxiv - Bioengineering 2023Quote: ErCas12a (1 μg) or LbCas12aU (1 μg) protein and 1 μg crRNA were assembled in 1X Nuclease Reaction Buffer (New England BioLabs, Ipswich, MA, USA) at room temperature for 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... corresponding to a portion of PfRNF1 (RP2; Fig. 1A) was expressed as a maltose-binding fusion protein using the pMAL™c5X-vector (New England Biolabs, Ipswich, USA). To this end ...
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bioRxiv - Biophysics 2021Quote: ... The Biotin-handle and Cosmid-I95 DNA were both digested for 2 hours at 37°C with SpeI-HF (NEB, R3133L) and subsequently heat inactivated for 20 minutes at 80°C ...
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bioRxiv - Cell Biology 2019Quote: ... stained with oligo-conjugated WGA at a concentration of 2-5 μg/mL in 1× HBSS with 2000× diluted murine RNase inhibitor (New England Biolabs, M0314L) at 37 °C for 20 min ...
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bioRxiv - Developmental Biology 2021Quote: ... The calibrated small RNA samples were ligated to an adenylated and fluorescently labeled 3’ adaptor using T4 RNA ligase 2 truncated KQ (NEB, M0373S) and were subsequently separated on a 12% polyacrylamide UREA gel ...
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bioRxiv - Evolutionary Biology 2021Quote: 5’ adapter ligation was performed by adding 3 uL of 10uM 5’ adaptor (which was previously denatured by heating to 70 C for 2 minutes and placed on ice, NEB E7330L), 2 uL of 10X T4 RNA ligation buffer (NEB B0216L) ...
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bioRxiv - Neuroscience 2021Quote: ... PCR products were run on 2% agarose gels and the Quick load 100pb DNA ladder (New England Biolabs Inc., Ipswich, MA) was used for fragment size visualization ...
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bioRxiv - Molecular Biology 2021Quote: ... All these reactions were performed in a single step by adding 2 µL of enzyme mix (1 µL of Thermolabile USER II (New England Biolabs, M5508L), 0.5 µL of Exonuclease I (New England Biolabs ...