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Citations for New England Biolabs :
3751 - 3800 of 6023 citations for 7 Oxa 1 2 diazaspiro 4.4 non 1 en 6 one 4 methyl cis 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... The template was eliminated after the PCR reactions by addition of 1 µL DpnI (10,000 U/mL, NEB) to 25 µL PCR reactions and incubation at 37 °C for at least 1 h ...
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bioRxiv - Microbiology 2021Quote: ... the cap-1 structure was added to the 5’ end using the Vaccinia capping enzyme (New England Biolabs) and Vaccinia 2’-O-methyltransferase (New England Biolabs) ...
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bioRxiv - Physiology 2020Quote: ... and analysed by Western blotting using antibodies for TCF4/TCF7L2 (C48H11) (#2569, 1:500, Cell signalling, NEB, UK), phospho-AKT (#9271 ...
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bioRxiv - Plant Biology 2020Quote: ... PCR enrichment of adaptor ligated DNA was conducted using NEBNext Multiplex Oligos for Illumina (Set 1, NEB#E7335). The PCR reaction was purified using Agencourt AMPure XP Beads ...
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bioRxiv - Biophysics 2020Quote: Each reverse transcription (RT) reaction was performed using 50 ng total RNA in 1 mM dNTPs (NEB N0447S), 10% DMSO (Fisher ...
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bioRxiv - Microbiology 2021Quote: ... 100ng of vector was digested for 1 hour at 37°C with 10 U of BamHI-HF (NEB) to create a single double-stranded cut or 10 U Nb.BtsI (NEB ...
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bioRxiv - Cell Biology 2021Quote: SNAP-GCGR was detected with an anti-SNAP-tag rabbit polyclonal antibody (P9310S, New England Biolabs, 1/500) followed by goat anti-rabbit IgG H&L HRP (ab6271 ...
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bioRxiv - Biochemistry 2022Quote: 3’-PUA-DNA was generated by incubation of 1 μM 5’-FAM-labeled AP-DNA (FAM_U_35) with 20 U EndoIII/Nth (NEB) in Buffer B pH 7.0 at 37 °C for 60 m ...
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bioRxiv - Biochemistry 2022Quote: AP-DNA was prepared by incubating 1 μM uracil-containing oligonucleotide with 0.6 U UDG (New England Biolabs) (55 ...
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bioRxiv - Biochemistry 2022Quote: ... and each RV segment was then transcribed using 1 μg of linearized DNA with HiScribe T7 from NEB at 37°C for 3 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Complexes of TtAgo programmed with ssDNA guides were prepared by combining final concentrations of 1 pmol TtAgo (NEB) and 2 pmol 5’-phosphorylated ssDNA guides and incubating at 70 °C for 20 minutes ...
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bioRxiv - Neuroscience 2021Quote: U251 MG (KO) cells and NSC-34 cells were collected into 1× SDS Blue loading buffer (NEB; #B7703S) and heat-treated at 95°C for 5 min ...
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bioRxiv - Bioengineering 2021Quote: ... fixed cells were incubated in DNAse I buffer (New Englab BioLabs, M0303S, 1:100 dilution in PBS buffer) for 30 min at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... 50 nM of each of the 27f and 534r primer mixes and 0.025U.μl−1 Taq DNA polymerase (NEB). The cycling parameters were 95 °C for 2 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... Around 500ng of RNA were ligated overnight at 16°C using 20units of T4 RNA ligase 1 (NEB) in the presence of 10% PEG 8000 to favour intramolecular ligations ...
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bioRxiv - Molecular Biology 2020Quote: ... Around 500ng of RNA were ligated overnight at 16°C using 20units of T4 RNA ligase 1 (NEB) in the presence of 10% PEG 8000 to favour intramolecular ligations ...
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bioRxiv - Immunology 2021Quote: ... 1 µL of ProtoScript II reverse transcriptase 200 U / µL (cat. n° M0314L NEB, New England Biolabs, USA), 2 µL of random primer mix 60 µM (cat ...
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bioRxiv - Immunology 2021Quote: ... 1 µL of ProtoScript II reverse transcriptase 200 U / µL (cat. n° M0314L NEB, New England Biolabs, USA), 2 µL of random primer mix 60 µM (cat ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The reaction was incubated for 1 hour at 37 °C and stopped by adding 6X loading dye (NEB) with EDTA and run 2% agarose gel ...
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bioRxiv - Genomics 2019Quote: ... RNA samples were ligated with the TIF-seq DNA/ RNA 5oligo cap using T4 RNA ligase 1 (NEB). Full-length cDNA (FlcDNA ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Up to 1 μg of DNA-free RNA was reverse transcribed using ProtoScript II Reverse Transcriptase (NEB, M0368), and anchored oligo-dT primers according to product instructions.
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bioRxiv - Synthetic Biology 2020Quote: ... One acceptor and one or more donors were mixed with a ratio of 1:1.1 in distilled H2O with 0.5 ul (7.5 U) of CRE recombinase (NEB # M0298M) and 1 ul Cre buffer (provided with CRE recombinase ...
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bioRxiv - Biochemistry 2019Quote: ... transferred at 300 mA for 90 minutes and probed overnight with α-MBP (1:10000, New England BioLabs) or α-ORC (1:1000 ...
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bioRxiv - Genomics 2019Quote: ... we transfected K562 cells as previously described with either 1 µg of pUC19 plasmid (New England BioLabs #N3041S); 0.5 µg of PB-SRT-tdTomato plasmid and 0.5 µg pUC19 ...
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bioRxiv - Molecular Biology 2019Quote: ... Annealed primers were extended at 60°C using 0.08 U μl−1 Bst 2.0 WarmStart DNA polymerase (New England Biolabs) and 0.8 mM dNTPs in isothermal amplification buffer (20 mM Tris-HCl ...
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bioRxiv - Bioengineering 2019Quote: ... at 15 units/mL was injected over the flow cell 1 in Neb2.1 buffer 1x (New England BioLabs) with a flow rate of 5 μL/min for 15 min at 12 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... A ligation mastermix was made consisting of 1 μl T4 DNA Ligase (NEB, cat#M0202S, 400 U/μl), 1 μl of T4 DNA ligase buffer (10x stock ...
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bioRxiv - Molecular Biology 2021Quote: ... Promoters (Supplemental Figure 1, Supplemental Table 3) were amplified from genomic DNA using Q5 polymerase (New England Biolabs) and inserted between the enhancer and 24xMS2 sequences using restriction enzyme-mediated ligation ...
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bioRxiv - Microbiology 2021Quote: ... The ~1 kb fragment was then digested with restriction enzymes EcoRI and HindIII (New England Biolabs, Ipswitch MA), purified using Fisher’s GeneJET Gel Extraction and DNA Clean Up Kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg of RNA was used for cDNA synthesis using the ProtoScript® II kit (New England BioLabs). qPCR reactions were carried out with FastStart Essential DNA Green Master (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... The probes were subjected to a 1:30 dilution of uracil-specific excision reagent (USER) enzyme (NEB, N5505S) for about 24h at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Up to 1 μg of DNA-free RNA was reverse transcribed using ProtoScript II Reverse Transcriptase (NEB, M0368) and anchored oligo dT primers according to the product instructions.
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bioRxiv - Biophysics 2019Quote: ... The coverslips were incubated in staining solution (1 µM benzylguanine Alexa Fluor 647 (S9136S, NEB, Ipswich, MA, USA); 1 mM DTT ...
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bioRxiv - Cancer Biology 2019Quote: ... and then resuspended in sorting buffer (PBS with 0.5% BSA, 2.5 mM MgCl2, 0.5 mM CaCl2, 1 µg/mL DAPI and 100 U/mL DNaseI, NEB) and incubated for 30 minutes at room temperature before sorting ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... was detected by addition of 1 nM Europium-labeled anti-p53 phosphoserine 15 antibody (New England Biolabs/ Cisbio) and 40 nM streptavidin-conjugated APC (Prozyme ...
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bioRxiv - Molecular Biology 2021Quote: ... mRNA was isolated from 1 µg total RNA using the NEB Poly(A) mRNA Magnetic Isolation Module (NEB) and prepared using the Stranded RNA-seq protocol (67) ...
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bioRxiv - Biochemistry 2021Quote: Each cyclization reaction contained the following components: 1 µL 10X T4 DNA ligase reaction buffer (New England BioLabs), 2 µL water ...
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bioRxiv - Biochemistry 2021Quote: ... The following day cells were treated with 1 µM SNAP-cell TMR-Star (New England BioLabs cat. # S9105S) in complete culture media for 45 minutes at 37 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 μ L of Protoscript II Reverse Transcriptase (200U/μ L, Catalog No. M0368, New England BioLabs Inc.), 2 μ L of 0.1M dithiothreitol (DTT) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 μg of DNA library was used as a template for mRNA synthesis by HiScribe T7 kit (NEB). The product was purified by NH4Ac precipitation and dissolved in RNAse-free water to a final concentration of 3 μg/ul.
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bioRxiv - Biochemistry 2021Quote: ... 50 μl of lysed cells were aliquoted to 8 tubes containing 450 μl of digestion mix (1× NEB 1 buffer ...
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bioRxiv - Biophysics 2021Quote: ... The phosphorylated 5’-ends were subjected to RNA ligation by 20 units of T4 RNA ligase 1 (NEB) in the presence of 10 mM DNA/RNA rP5_RND oligo (Supplemental Table 3 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 ug of RNA was reverse-transcribed using the WarmStart® RTx reverse transcriptase (New England BioLabs, M0380L) and random primer mix (New England BioLabs ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl of 100 μM SARS_139 and SARS_140 primers were mixed with 1 μl T4 Polynucleotide kinase (PNK; NEB) in 1x PNK reaction buffer (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... For size reference 1 kb and 100 bp DNA ladders (New England Biolabs, catalog no. N3232 and N3231) were combined and loaded onto the gel ...
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bioRxiv - Biophysics 2022Quote: ... 1 μL of 100 mM 18O4-γ-ATP followed by addition of 20 U of T4-PNK (NEB) and incubation for 1 h at 37 °C ...
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bioRxiv - Biophysics 2022Quote: ... 40 μL of pooled oligonucleotides (100 μM) were mixed with 12 μL ddUTPs (1 mM, New England Biolabs), 2.4 μL TdT (20000 U/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1, NEB, E7335L). Conditions for PCR used are as follows ...
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bioRxiv - Developmental Biology 2022Quote: ... and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1, NEB, E7335L). Conditions for PCR used are as follows ...
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bioRxiv - Microbiology 2022Quote: ... The digestion product was then treated with Antarctic phosphatase for 1 hour at 37°C (New England Biolabs). PCR products were purified using the Zymogen Clean and Concentrator PCR-cleanup kit (Zymo Research ...