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Citations for New England Biolabs :
3551 - 3600 of 5258 citations for 6 Chlorotetrazolo 1 5 b pyridazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... either with or without 1 µL of PNGase F (500 Units, NEB).
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bioRxiv - Biochemistry 2024Quote: ... coli exonuclease III (Xth) was performed in NEBuffer 1 (New England Biolabs) with 1 nM enzyme ...
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bioRxiv - Genomics 2024Quote: ... 10 µg of RNA was treated with 1 µL Xrn1 (NEB, M0338S) for 1 h at 37 °C to remove decapped ...
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bioRxiv - Neuroscience 2024Quote: ... with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (NEB). All libraries were sequenced 150bp paired end using an Illumina Novaseq 6000 with 15 million reads on average per sample.
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bioRxiv - Cancer Biology 2021Quote: ... and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) (NEB: E7335L). Samples with total RNA concentration of >10 ng/µl (0.5 µg total amount ...
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bioRxiv - Genetics 2021Quote: ... then a final 1 min extension (72 °C)—using Phusion Flash polymerase (NEB) from cDNA clones using primers containing an EagI restriction site (forward primers ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 1 μL of 10 mM dNTPs (New England Biolabs, Ipswich, MA, USA) to 10 μL RNA and incubating at 65°C for 5 min on a C1000 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) by NEB following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1 µl of T4 or T7 DNA Ligase (New England Biolabs, Ipswich, USA), 1 µl of BsaI-HFV2 (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL Bst 2.0 WarmStart® DNA polymerase (0.32 u; Biolabs, Ipswich, MA), 1 μL Hydroxy Naphthol Blue (HNB ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μL of 1× PNK mix (2 μL of 10× PNK buffer (NEB), 2 μL ATP (SCP801 ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was phosphorylated for 1 h at 37oC with T4 Polynucleotide kinase (NEB or made in-house by the Molecular Biology Service ...
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bioRxiv - Cell Biology 2022Quote: ... 1/10 T7 RNA polymerase mix (HighScribe T7 High Yield RNA synthesis NEB)) at 37°C ...
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bioRxiv - Genomics 2022Quote: ... 1 or 2 μl of 1U/μl USER® II enzyme (M5505S, NEB) and nuclease-free water to made up to 10 μl ...
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bioRxiv - Genomics 2019Quote: ... Bead-nuclei were subsequently washed with 200 µL 1× RNA ligase buffer (NEB) supplemented with 0.1% Triton X-100 and with 200 µL 1× RNA ligase buffer (NEB ...
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bioRxiv - Genomics 2019Quote: ... and resuspended in 95 µL PNK solution (1× T4 DNA ligase buffer (NEB), 0.1% Triton X-100) ...
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bioRxiv - Microbiology 2021Quote: ... 10 μM dNTPs (0.5 μl per reaction) in 1× Klenow reaction buffer (NEB). Next ...
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bioRxiv - Immunology 2021Quote: ... The parental caspase-1 plasmid (methylated) was digested using DpnI (NEB; Cat,# R0176S). Plasmids were transformed in E.coli DH5α competent cells (NEB ...
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bioRxiv - Microbiology 2019Quote: ... and 1 µL used in PCR with Taq DNA Polymerase (New England Biolabs). Primers amplified the repB region of plasmids pAM714 and pAM771 (pAD1 repB-For ...
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bioRxiv - Neuroscience 2019Quote: ... 1 µM NGFR121W-SNAP was coupled with 3 µM BG549 surface (NEB # S9112) for 1 hour at 37°C in calcium imaging (CIB ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 mM EGTA and 2 mM Vanadyl Ribonucleoside complex (VRC, New England Biolabs), washed three times with 70% ice-cold ethanol and kept at −20°C.
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bioRxiv - Microbiology 2019Quote: ... 1 µl of 200 mM dNTPs (New England Biolabs Inc., Ipswich, MA, USA), 2 µl of 5 µM of each Primer ...
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bioRxiv - Microbiology 2019Quote: ... The Quick-Load 1 kb Extend DNA Ladder (New England BioLabs, Ipswich, MA) was used as a molecular marker.
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bioRxiv - Genetics 2019Quote: ... Each PCR reaction was treated with 1 µl of Exonuclease I (M0293, NEB) at 37 °C for 15 min followed by 80 °C for 20 min to inactivate the Exonuclease I enzyme.
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bioRxiv - Biochemistry 2021Quote: ... The parental plasmid molecules were inactivated by DpnI treatment (1 h, NEB, USA) and the crude reaction mixture was transformed to electrocompetent E ...
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bioRxiv - Genomics 2021Quote: ... 1 µL of 10 U/µL polynucleotide T4 kinase and PNK buffer (NEB) in 20 µL ...
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bioRxiv - Developmental Biology 2021Quote: Plasmid pJM1 (Pceh-23_L::mKate2::rgl-1) was generated using Gibson Assembly (NEB) of PCR products ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by the addition of 1 µl RNase H (5U final, NEB M0297L) and incubation for 20 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... in 1 x Cutsmart buffer (New England Biolabs GmbH, Frankfurt am Main, Germany) for 60 min and then the enzymes heat inactivated at 80°C for 20 min according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ~1 μg of tsetse saliva was treated with PNGase F (New England Biolabs). Samples were run on a 12.5% polyacrylamide gel ...
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bioRxiv - Bioengineering 2020Quote: ... a 10x Cas12-gRNA complex was prepared using 1 μM of LbCas12a (NEB) and 0.5 μM of Cy5-labeled gRNA ...
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bioRxiv - Biochemistry 2020Quote: ... The template plasmid molecules were inactivated by DpnI treatment (1 h, NEB, USA) and directly transformed to electrocompetent E ...
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bioRxiv - Systems Biology 2020Quote: ... MnCl2 to a concentration of 1 mM and NEBuffer for Protein MetalloPhosphatases (NEB) diluted to a 1X concentration were added to both tubes ...
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bioRxiv - Cell Biology 2021Quote: ... containing 1% Triton X-100 and 30 Units of T4 DNA ligase (NEB), followed by ligation reaction at 16°C for 4.5 h and then at room temperature for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM PMSF) and proteins were purified by amylose beads (New England Biolabs) and eluted in wash buffer (20mM Tris pH:7.6 ...
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bioRxiv - Systems Biology 2021Quote: ... 25 μL of amplicons were digested with 1 μL of DpnI (NEB R0176S). 100-200 ng of purified digestion was ligated by T4 DNA ligase (Promega M1801) ...
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bioRxiv - Genomics 2021Quote: ... for 1 hour at 37°C in the presence of CutSmart buffer (NEB). To achieve combined DNA purification and selection of high molecular weight fragments ...
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bioRxiv - Genetics 2021Quote: ... Worms were grown on OP50-1 bacteria or dam-dcm- bacteria (NEB C2925) on standard nematode growth medium (NGM ...
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bioRxiv - Microbiology 2021Quote: ... the cDNA was treated with 1 ul of RNase H (New England Biolabs) and incubated at 37 C of 20 minutes.
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bioRxiv - Microbiology 2020Quote: ... but with NEBNext Multiplex Oligos for Illumina Dual Index Primers Set 1 (NEB). Barcoded amplicons were then purified with AMPure XP magnetic beads (Beckman Coulter) ...
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bioRxiv - Microbiology 2021Quote: ... 1 μl BSA (20 mg/ml) (New England Biolabs Inc., Ipswich, MA, USA), 10 μl Brilliant III Ultra-Fast SYBR® Green Low ROX qPCR Master Mix (Agilent Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR round 1 and 2 were performed using Q5-High Fidelity polymerase (NEB) for 6 (FS231 and FS232 ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μL 400 U μl-1 T4 DNA Ligase (NEB, high concentration formula) and 664 μL H2O and incubated for 120 mins at 23°C with 300 rpm slow rotation ...
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bioRxiv - Genomics 2022Quote: ... We ligated a 3’ adapter ligation using T4 RNA Ligase 1 (NEB, M0204L). We performed a second bead binding followed by a 5’ decapping with RppH (NEB ...
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bioRxiv - Immunology 2022Quote: ... Restriction digestion was quenched by adding 1 μl proteinase K (New England Biolabs) and incubation for 2 mins at 37°C ...
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bioRxiv - Genomics 2022Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (NEB) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... pcDNA3.1.mNG2(1-10) was generated through gibson assembly (NEBuilder, New England Biolabs) of a pcDNA3.1 vector (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were stopped by the addition of 1 μL of Proteinase K (NEB) and incubated at room temperature for 15 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) oligos (NEB, E7600S), and an Illumina NextSeq 550 75 cycle high-output kit (Illumina ...
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bioRxiv - Bioengineering 2022Quote: ... cas12a (1 μM) and ATP are purchased from NEB (New England Biolabs Inc). SYBR Green II dye ...