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Citations for New England Biolabs :
3501 - 3550 of 9547 citations for QuantiChrom Phosphate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The PCR products were then individually ligated using a KLD Enzyme reaction kit (New England Biolabs) for 3 hours and subsequently transformed into ElectroMAX DH10B E ...
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bioRxiv - Systems Biology 2019Quote: ... RT-qPCR was performed with the Luna Universal One-Step RT-qPCR Kit (New England Biolabs). 1 μl of 10-fold diluted RNA was added to 4 μl of rtPCR mix and subjected to a reverse transcription step at 55°C and 45 cycles of PCR (10 seconds at 95°C and 30 seconds at 60°C) ...
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bioRxiv - Cancer Biology 2020Quote: ... ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Preparation Kit (NEB, #E7645). Libraries were analyzed for fragment size using the Bioanalyzer High-Sensitivity DNA Kit (Agilent ...
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bioRxiv - Zoology 2020Quote: ... we prepared PCR-free libraries using NEBNext Ultra II DNA library prep kit (New England Biolabs), but with TruSeq DNA Single Indexes (Set B ...
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bioRxiv - Microbiology 2019Quote: ... The Illumina sequencing library were prepared with the NEBNext® Ultra DNA Library Prep kit (NEB) by selecting 400-bp fragments ...
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bioRxiv - Developmental Biology 2019Quote: Pceh-23_L::acy-2 fragment(anti-sense) was generated with a Gibson assembly cloning kit (NEB) by assembly of the following two DNA fragments ...
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bioRxiv - Cancer Biology 2020Quote: ... NEB Next Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) was used for the construction of sequencing libraries ...
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bioRxiv - Developmental Biology 2019Quote: ... before final assembly with pJET1.2 vector using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). Small Guide encoding plasmids for CRISPR were generated by an overlap extension PCR method performed on template pML2840.
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bioRxiv - Developmental Biology 2020Quote: ... Dppa3 and mutant Dppa3 (R107E) mRNA were synthesized using HiScribe T7 ARCA mRNA kit (NEB, E2060S), and purified using RNeasy mini kit (QIAGEN ...
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bioRxiv - Molecular Biology 2019Quote: ... Associated DNA was then purified by extraction using Monarch PCR & DNA Cleanup Kit (New England Biolabs). Immunoprecipitated DNA and total input were used as a template for real-time qPCR ...
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bioRxiv - Cancer Biology 2019Quote: ... and converted into libraries using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs).
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bioRxiv - Synthetic Biology 2019Quote: ... and subsequently cloned in to the pMini-T plasmid using the PCR Cloning Kit (NEB #E1202). Plasmids were extracted from E.coli JM109 transformed cells using the QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Immunology 2019Quote: ... and subjected to in vitro transcription (IVT) using HiScribe T7 High Yield RNA Synthesis Kit (NEB), then treated with ExoSAP-IT (Affymetrix 78200) ...
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bioRxiv - Microbiology 2020Quote: ... 1µL Random Primer Mix (ProtoScript II First Strand cDNA Synthesis Kit, New England Biolabs, https://www.neb.ca) was added to 7 µL extracted RNA and denatured on SimpliAmp thermal cycler (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... The MyoXIa-CCT bait was mutagenized with the Q5 Site-Directed Mutagenesis Kit (New England BioLabs) to create the V1418R and V1422R mutants ...
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bioRxiv - Microbiology 2020Quote: ... library preparation through Next® Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs) and HiSeq 2500 paired-end 150 bp sequencing.
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bioRxiv - Cancer Biology 2019Quote: ... After determining accurate concentrations by quantitative PCR using the NEBnext library quant kit for Illumina (NEB), pooled libraries were sequenced on an Illumina HiSeq 2500 platform using a 50 bp single read on a high output standard v4 flow cell with a 15-20% PhiX spike-in ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep kit (NEB). To quantitate the consistency of spike-in cell mixing for each individual sample ...
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bioRxiv - Developmental Biology 2020Quote: ... The sequencing-ready cDNA libraries were quantified with the NEBNext library quantification kit for Illumina (NEB). mRNA-seq libraries were sequenced with single-end 50 at ≈20 million reads per sample with biological triplicates ...
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bioRxiv - Microbiology 2020Quote: ... Gibson assembly was performed using NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs Inc.) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2019Quote: ... and subsequently processed with the "NEBNext Ultra II DNA Library Prep Kit for Illumina" (NEB #E7645). Briefly ...
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bioRxiv - Developmental Biology 2019Quote: ... The R6C mutation was introduced into the construct using the Q5 site-directed mutagenesis kit (NEB). The pcDNA3-HA-human OCRL plasmid was a gift from Pietro De Camilli (Addgene plasmid # 22207 ...
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bioRxiv - Cell Biology 2019Quote: ... and detected using components based on the Phototope-Star detection kit (New England BioLabs, Cat# N7020). All T ...
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bioRxiv - Genomics 2019Quote: ... Libraries were constructed using NEBNext Ultra II RNA Library Prep kit for Illumina (New England Biolabs). The mRNA was fragmented ...
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bioRxiv - Genomics 2019Quote: ... Libraries were generated using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). The DNA was fragmented to a size of 350 bp ...
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bioRxiv - Neuroscience 2019Quote: ... DNA libraries were constructed using NEBNext UltraII DNA Library Prep Kit for Illumina (New England BioLabs) and quantified using KAPA Library Quantification Kit for Illumina (KAPA Biosystems) ...
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bioRxiv - Genetics 2021Quote: gDNA was extracted with the Monarch Genomic DNA purification kit (New England Biolabs; Ipswich, MA, USA) from 1ml of culture.
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bioRxiv - Genetics 2021Quote: ... DNA from the excised fragments was extracted using the Monarch® DNA Gel Extraction Kit (NEB). Before cloning ...
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bioRxiv - Evolutionary Biology 2021Quote: ... HYL1− knockout and transgenic ancFpHYL1 seedlings using the Monarch Total RNA Miniprep kit (New England BioLabs), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... smaller DNA fragments (<100 bp) were purified using the Monarch PCR Purification Kit (New England Biolabs) with a modified protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Circularized ssDNA was next purified and concentrated using a Monarch® PCR & DNA Cleanup Kit (NEB). 20 pmol of the purified product was then incubated with 25 pmol of a complementary primer in 1X T4 DNA ligase buffer (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... to remove DNA templates and cleaned up with Monarch RNA Cleanup Kit (50μg) (New England Biolabs) and eluted in nuclease-free water ...
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bioRxiv - Plant Biology 2019Quote: ... sequencing libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The resistance marker was assembled left of the amplified fimS regions using NEBuilder assembly kit (NEB). The assembled DNA fragments were PCR amplified using primers 130 and 131 and integrated into the chromosome of the respective strains instead of the endogenous fimS using lambda red recombination (Datsenko & Wanner ...
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bioRxiv - Biochemistry 2020Quote: RNA was transcribed from PCR-amplified inserts using the Hiscribe T7 high yield synthesis kit (NEB). 0.1µg/µl of the RNA of interest was heated to 90°Cfor 2 mins and cooled at 4°Cfor 2 mins before adding chilled 10X RNA structure buffer (500mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... All RNA synthesis was performed using the HiScribe T7 High Yield RNA synthesis kit (NEB E2040S) according to the manufacture’s protocol ...
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bioRxiv - Bioengineering 2020Quote: IVT was carried out using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB E2050S) with the addition of RNAsin (Promega N2111) ...
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bioRxiv - Bioengineering 2021Quote: ... The amplicons and the linearized plasmid were assembled using an NEBuilder HiFi DNA assembly kit (NEB). The assembled plasmid was named pLenti-NG-PE2-BSD.
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bioRxiv - Bioengineering 2021Quote: ... we performed in vitro RNA transcription using the HiScribe T7 High Yield RNA Synthesis Kit (NEB). After transcription ...
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bioRxiv - Bioengineering 2020Quote: ... cDNA was synthesized with the First Strand cDNA Synthesis Kit (E6300S; New England BioLabs, Ipswich, MA) and analyzed for expression of collagen I (primer sequences ...
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bioRxiv - Biochemistry 2020Quote: ... The samples were prepared using a Luna Universal One-Step RT-qPCR Kit (New England Biolabs). Quantitative RT-PCR was performed with an Mx3000P qPCR system (Agilent ...
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bioRxiv - Biochemistry 2020Quote: ... Mutagenesis of WDR5 N130A was carried out using a Q5 site-Directed MUtagenesis Kit (NEB, E0552S). Lentivirus packaging and infection were carried out as described above ...
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bioRxiv - Biochemistry 2020Quote: ... Sequencing libraries were generated using NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
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bioRxiv - Neuroscience 2021Quote: ... RNA-Seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB) and sequenced with the Novaseq 6000 platform (ILLUMINA ...
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bioRxiv - Microbiology 2021Quote: ... 1 µg of RNA was used for cDNA synthesis using the LunaScript RT SuperMix Kit (NEB) with and without reverse transcriptase (RT) ...
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bioRxiv - Cell Biology 2021Quote: ... were generated from pCMV6M-myc-PAK1 using Q5® Site-Directed Mutagenesis Kits (New England BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... mRNA expression was determined using SYBR green Luna One Step RT-qPCR Kit (New England BioLabs) on a C1000 Touch (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... Indexed sequencing libraries were generated using the NEBNext Ultra II DNA Library Prep kit (NEB, # E7645), pooled and sequenced on an Illumina HiSeq instrument as paired end 150 bp reads (Genewiz ...
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bioRxiv - Plant Biology 2021Quote: ... The K107E mutation in PDF2 was generated using Q5 Site-Directed Mutagenesis Kit (New England Biolabs) with described primers (Supplemental Table 5) ...
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bioRxiv - Plant Biology 2021Quote: ... Libraries for WGBS were prepared using the NEBNext UltraII DNA Library Prep kit (New England Biolabs). Adapter-ligated DNA was treated with sodium bisulfite using the EpiTect Plus Bisulfite kit (QIAGEN ...