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Citations for New England Biolabs :
301 - 350 of 9140 citations for Recombinant Human KIT His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... We put the mutagenized genes in a pIVEX vector via Gibson assembly (NEB Hi-Fi DNA assembly) using 125ng of gene DNA ...
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bioRxiv - Biochemistry 2021Quote: ... and C-terminal additions) were assembled with the pcDNA5 backbone using Hi-Fi DNA assembly (NEB; E2621S).
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bioRxiv - Plant Biology 2022Quote: ... His-TAD1 fusion proteins were purified according to the manufacturer’s protocol (New England Biolabs, Ipswich, MA, USA). The bait protein GST-WL1 was incubated with GST beads (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... the product was cloned into the pET1-5b N-terminal 6×His expression plasmid (New England Biolabs).
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bioRxiv - Molecular Biology 2021Quote: ... The His-GST tags were cleaved by incubating the pooled fractions with TEV protease (New England Biolabs) at 4°C for 16h ...
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bioRxiv - Genetics 2019Quote: ... 2012) by removing cbr-Pmyo-2∷gfp∷his-72 UTR by cutting using KpnI and ApaI (NEB). The digested pZZ0031 backbone carrying cbr-unc-119(+ ...
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bioRxiv - Bioengineering 2022Quote: ... The t-NLuc-His tag was double-digested with NdeI and XhoI restriction enzymes (New England BioLabs), and the RNA sensor region were digested with SgrAI and SacII restriction enzymes ...
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bioRxiv - Microbiology 2023Quote: ... All 3 fragments were amplified from pTRL2-His-GrgA 36 using Q5 DNA polymerase (New England Biolabs). Fragment 1 was generated using primers pgp3-pgp4-F and His-RBS-R (Table S1) ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were constructed in the pUC19 backbone using NEBuilder Hi-Fi DNA Assembly Mastermix (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... All 3 fragments were amplified from pTRL2-His-GrgA (28) using Q5 DNA polymerase (New England Biolabs). Fragment 1 was generated using primers pgp3-pgp4-F and His-RBS-R (sTable 5) ...
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bioRxiv - Molecular Biology 2021Quote: DNAse-treated RNA with high RIN value was used to deplete ribosomal RNA using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6350) as per manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: PME measurements were carried out using a transient expression vector we created by inserting a synthetic gene block encoding the cDNA sequence of the human β2AR (Integrated DNA Technologies, Coralville, IA) into a modified pcDNA5 FRT using the NEBuilder kit (New England Biolabs, Ipswitch, MA). The final construct was designed to generate β2AR bearing an N-terminal influenza hemagglutinin tag from a transcript that also features a downstream internal ribosome entry site (IRES)-dasher GFP cassette that facilitates the unambiguous identification of positively-transfected cells ...
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bioRxiv - Cell Biology 2021Quote: ... plasmid encoding GFP-labeled dominant negative mutant version of human Rab5B isoform was introduced to plasmid #1008 using Q5 Site-Directed Mutagenisis kit (NEB, Cat# E0554S) according to manufacturer’s instructions using forward primer #1554 (AGTGGGAAAGaacAGCCTGGTATTAC ...
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bioRxiv - Microbiology 2021Quote: ... ribosomal RNA depletion was carried on the extracted RNA using Nebnext rRNA depletion kit (Human/mouse/rat) (New England BioLabs. In, USA). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... The G4 sequence and larger 5′ UTR (three human β-globin 5′ UTR sequences in tandem) were inserted using the Q5 Site-Directed Mutagenesis Kit (NEB # E0552S). pcDNA3.1(+)/mEGFP was a kind gift from Jeremy Wilusz (Baylor College of Medicine) ...
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bioRxiv - Microbiology 2023Quote: ... RNA-seq libraries were prepared from RNA samples (150ng) using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB Cat# E7405) in conjunction with NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB Cat# E7765) ...
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bioRxiv - Biochemistry 2023Quote: ... An mRNA transcript library for Illumina sequencing was created using the NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs, Ipswich, MA). A NextSeq 500 sequencer (Illumina ...
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bioRxiv - Biochemistry 2019Quote: ... Quantification of the ratio of MS2-tagged rRNA to WT rRNA was done via semi-quantitative PCR with Q5 DNA polymerase (NEB). Primers MS2_quant_F/R bind outside the MS2 tagged region of 23S cDNA ...
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bioRxiv - Microbiology 2021Quote: The coding sequence of CTSL was tagged with a 3’V5 and cloned into CSIN using NEBuilder HiFi DNA Assembly (NEB) with primers CTSL_3’_V5_F ...
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bioRxiv - Plant Biology 2021Quote: Blunt ended Myc tagged GUX and AtMAGT1 CDSs were amplified from a synthetic construct (synthesised by Genewiz) with Q5 DNA Polymerase (NEB) using primers detailed in Table S1 ...
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bioRxiv - Plant Biology 2021Quote: ... SPL8 and SPL15 were amplified from cDNA to generate the respective tagged constructs using Q5 high fidelity DNA polymerase (NEB). The primers (listed in Table 5 ...
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bioRxiv - Biochemistry 2022Quote: 30 uL of tagged Fluc-WT proteins were incubated with Factor Xa (NEW ENGLAND BioLabs, final concentration 67 ug/mL) for 2 hours or overnight on ice.
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bioRxiv - Cell Biology 2022Quote: ... was expressed as maltose-binding protein-tagged fusion protein using the pMAL(tm)c5X-vector (New England Biolabs, Ipswich, USA). The coding DNA sequence was amplified by PCR using gene-specific primers (for primer sequences ...
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bioRxiv - Cell Biology 2019Quote: ... To visualize SNAP-tagged dCENP-A proteins cells were labelled with 3 µM SNAP-Cell TMR Star (New England Biolabs) for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... harboring the p6- GLuc / p6 genomes or pTM-ORF1 vectors and epitope-tagged inserts were restricted by the suitable restriction enzymes (Table 1) and subsequently ligated using the T4 DNA ligase (NEB). Finally ...
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bioRxiv - Genetics 2020Quote: ... and T3 (5’-AATTAACCCTCACTAAAGGG-3’) promoter-tagged PCR fragment from each gene using corresponding T7 and T3 RNA polymerase (T3:M0378S; T7:M0251S, BioLabs). Primers used for PCR are listed in Supplemental table 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids and siRNAs Bacterial expression constructs for His6-tagged p97 fragments were generated by Q5 Site-Directed mutagenesis (New England Biolabs) of pET15b-His6-p97 plasmid ...
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bioRxiv - Cell Biology 2023Quote: ... the SNAP-tagged fragment was assembled into a ApaI digested MBO2-HA plasmid using NEBuilder (New England Biolabs, Ipswich, MA). The final N-SNAP-MBO2-HA construct encodes an MBO2 polypeptide with a SNAP tag at its N-terminus and a 2-HA tag located between amino acids 885 and 886 of the original MBO2 sequence (Supplemental Figure S1).
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bioRxiv - Cell Biology 2023Quote: ... To remove RNA secondary structure and anneal the mRNA capture primer 1 μL of tagged random hexamer (100 μM) and 0.5 μL of 10 mM dNTPs (dNTP solution set NEB - N0446S) were added to the sample ...
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bioRxiv - Molecular Biology 2023Quote: Expression vectors encoding His6/SUMO-tagged SAHS proteins were obtained from Twist Bioscience and transformed into LEMO21(DE3) competent cells (New England Biolabs) according to the provider’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... myc-tagged AP3B1 immunoprecipitated and immobilised on Protein G-sepharose beads was incubated with CK2 (250 units; New England Biolabs) for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Then the ORF of the ZNRF3 short variant replaced RNF43 and was assembled into the C-terminal FLAG-tagged vector using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs) and the GeneArt Gibson Assembly HiFi Master Mix (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... The recombinant plasmids (LentiCRISPRv2.gRNA10, LentiCRISPRv2.gRNA11) were then transformed into competent Escherichia coli (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Recombinant proteins were expressed in Escherichia coli T7 Express lysY/Iq cells (New England Biolabs). For all proteins except PP1γ ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant plasmid was generated by a NEBuilder® HiFi DNA Assembly (New England Biolabs) reaction ...
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bioRxiv - Cell Biology 2020Quote: Recombinant MBP fusions to Nud1 or Nud17A were expressed from pMAL-c4X (New England Biolabs). Induction in Rosetta 2 cells (Novagen ...
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bioRxiv - Cancer Biology 2021Quote: Trastuzumab deglycosylation was achieved by adding 1.1 units of recombinant PNGaseF enzyme (New England BioLabs) per 1 μg of antibody ...
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bioRxiv - Biochemistry 2021Quote: ... recombinant SARS-CoV-2 Omicron RBD was digested with EndoH over night (New England Biolabs). Recombinant hACE2 protein was digested using EndoH (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... Ribonucleoprotein (RNP) complexes were formed from sgRNA and recombinant Cas9 2NLS protein (New England Biolabs) mixed at a sgRNA to Cas9 ratio of 4.5 ...
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bioRxiv - Cell Biology 2022Quote: ... The reaction was initiated by addition of 10 U (pmol/min) of recombinant CK1 (NEB), CDK1-cyclin B1 (NEB) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The reaction was started by adding 5000 units of recombinant Casein Kinase II (NEB, P6010S) and left to incubate for 20 min at 30°C with gentle agitation ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg of the sgRNA was complexed with 20 pmol recombinant Cas9 endonuclease (NEB # M0646T) and injected into the C ...
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bioRxiv - Genomics 2021Quote: ... The PCR reaction consisted of 25 µL NEBNext Hi-Fidelity 2x PCR Master Mix (New England Biolabs NEB.M0541S), 7.5 µL H2O ...
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bioRxiv - Genomics 2021Quote: ... The PCR reaction consisted of 25 µL NEBNext Hi-Fidelity 2x PCR Master Mix (New England Biolabs NEB.M0541S), 7.5 µL H2O ...
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bioRxiv - Biochemistry 2021Quote: ... fully assembled complexes containing His-Rpn12 and MBP-Rpn6 were purified using a HisTrap and amylose resin (NEB), and cleaved with HRV-protease ...
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bioRxiv - Cell Biology 2020Quote: ... Igκ-LaNtα31-Myc-His was excised from pGEM®-5Zf(+)-LaNtα31 using NdeI and NsiI (New England Biolabs) and inserted into phK14-mCherry ...
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bioRxiv - Microbiology 2022Quote: ... Tagmented DNA was amplified by PCR using 1x NEBNext Hi-Fidelity PCR Master Mix (New England Biolabs, NEB), using Nextera Index i5 and i7 series PCR primers ...
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bioRxiv - Microbiology 2022Quote: ... Tagmented DNA was amplified by PCR using 1x NEBNext Hi-Fidelity PCR Master Mix (New England Biolabs, NEB), using Nextera Index i5 and i7 series PCR primers ...
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bioRxiv - Microbiology 2023Quote: ... Purified PCR products were incubated together in a thermocycler with 1X Hi-Fi DNA Assembly master mix (NEB) at 50°C for one hour ...
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bioRxiv - Microbiology 2019Quote: ... falciparum and human histones (New England Biolabs) (6µg) ...